FastQCFastQC Report
Mon 15 Aug 2016
SRR2921516_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921516_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1503623
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27000.17956628756011314No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23800.15828435718261827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23600.080.075521
GTATCAA46200.058.7113341
TCAACGC62650.043.2465024
CAACGCA62950.042.9186635
TATCAAC65800.041.4490662
ATCAACG65350.041.368613
AACGCAG65800.040.9597476
GTACATG76550.037.302921
TACATGG78600.035.835522
ACGCAGA78300.034.1906057
ACATGGG80500.034.1748623
CGCAGAG81600.032.734998
CATGGGG71100.029.9829334
GCAGAGT88150.029.6932339
AGAGTAC83150.026.54275510-11
CAGAGTA86550.026.18739310-11
GTATAGA5100.025.7160951
GAGTACT50050.025.43445412-13
ATGGGGG37200.024.9555845
GTATAGG4350.024.6681041
Produced by FastQC (version 0.11.5)