##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921516_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1503623
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.215674407747155	31.0	31.0	34.0	28.0	34.0
2	31.258246249226037	33.0	31.0	34.0	28.0	34.0
3	31.340861372830823	33.0	31.0	34.0	28.0	34.0
4	34.86879224380047	37.0	35.0	37.0	32.0	37.0
5	34.52784973361009	37.0	35.0	37.0	30.0	37.0
6	34.50323651606819	37.0	35.0	37.0	30.0	37.0
7	34.34507519504557	37.0	35.0	37.0	30.0	37.0
8	34.315604376895	37.0	35.0	37.0	30.0	37.0
9	35.8837301637445	39.0	37.0	39.0	30.0	39.0
10-11	35.749311828829434	39.0	35.0	39.0	30.0	39.0
12-13	35.65523937848783	39.0	35.0	39.0	30.0	39.0
14-15	36.812101171636776	40.0	37.0	41.0	30.0	41.0
16-17	36.786207380440445	40.0	37.0	41.0	30.0	41.0
18-19	36.804096173043376	40.0	37.0	41.0	30.0	41.0
20-21	36.784528768181914	40.0	37.0	41.0	30.0	41.0
22-23	36.726118847610074	40.0	37.0	41.0	30.0	41.0
24-25	36.65936674286041	40.0	36.5	41.0	30.0	41.0
26-27	36.52727478895973	40.0	36.0	41.0	29.5	41.0
28-29	36.34084773909417	40.0	36.0	41.0	28.5	41.0
30-31	36.291858065485826	40.0	36.0	41.0	29.0	41.0
32-33	36.16748812701056	40.0	35.0	41.0	29.0	41.0
34-35	36.043394853630204	40.0	35.0	41.0	28.0	41.0
36-37	35.91089688040154	40.0	35.0	41.0	27.5	41.0
38-39	35.73197969171794	40.0	35.0	41.0	27.0	41.0
40-41	35.5261681285801	39.0	35.0	41.0	26.0	41.0
42-43	35.35312175990923	39.0	35.0	41.0	26.0	41.0
44-45	35.13803327030779	39.0	35.0	41.0	24.5	41.0
46-47	34.85758331709478	39.0	34.5	41.0	23.5	41.0
48-49	34.589710984734864	39.0	34.0	41.0	23.0	41.0
50-51	34.3623481417882	39.0	34.0	41.0	22.0	41.0
52-53	34.158967706665834	38.0	34.0	40.5	22.0	41.0
54-55	33.938402445293804	38.0	33.0	40.0	21.0	41.0
56-57	33.67461790621718	37.5	33.0	40.0	20.0	41.0
58-59	33.520520768836334	37.0	33.0	40.0	20.0	41.0
60-61	33.38723203888209	37.0	33.0	40.0	20.0	41.0
62-63	33.11043925239239	36.0	33.0	40.0	20.0	41.0
64-65	32.81374054533616	36.0	33.0	39.0	18.0	41.0
66-67	32.50173015443366	35.0	32.5	39.0	18.0	41.0
68-69	32.18288793134981	35.0	32.0	38.5	17.0	40.5
70-71	31.875565550673272	35.0	32.0	37.0	15.5	40.0
72-73	31.564213236961656	35.0	32.0	37.0	13.0	39.0
74-75	31.279538820568717	35.0	32.0	36.5	12.0	39.0
76-77	30.990956177180053	35.0	31.5	36.0	10.5	38.5
78-79	30.723266403879165	35.0	31.0	35.5	7.5	37.0
80-81	30.505565557323877	35.0	31.0	35.0	7.0	37.0
82-83	30.326118315561814	35.0	31.0	35.0	7.0	36.5
84-85	30.142851964887477	35.0	31.0	35.0	6.5	36.0
86-87	30.006379591160815	35.0	31.0	35.0	3.5	36.0
88-89	29.90408067713782	35.0	31.0	35.0	2.0	35.5
90-91	29.805966322675296	35.0	31.0	35.0	2.0	35.0
92-93	29.86937483664456	35.0	31.0	35.0	2.0	35.0
94-95	29.840754963178938	35.0	31.0	35.0	2.0	35.0
96-97	29.822376353647158	35.0	31.0	35.0	2.0	35.0
98-99	29.78533382370448	35.0	31.0	35.0	2.0	35.0
100-101	29.730904954233875	35.0	31.0	35.0	2.0	35.0
102-103	28.84721768688029	33.5	29.5	35.0	2.0	35.0
104-105	29.555071650274037	34.5	31.0	35.0	2.0	35.0
106-107	29.660362670696045	35.0	31.0	35.0	2.0	35.0
108-109	29.66830249337766	35.0	31.0	35.0	2.0	35.0
110-111	29.63854370410668	35.0	31.0	35.0	2.0	35.0
112-113	29.594472151596513	35.0	31.0	35.0	2.0	35.0
114-115	29.527692779373552	35.0	31.0	35.0	2.0	35.0
116-117	29.449086306873465	35.0	31.0	35.0	2.0	35.0
118-119	29.357922165330006	35.0	31.0	35.0	2.0	35.0
120-121	29.234542501677616	35.0	31.0	35.0	2.0	35.0
122-123	29.112942871983204	35.0	31.0	35.0	2.0	35.0
124-125	28.197029109025337	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	25030.0
3	9125.0
4	5309.0
5	4498.0
6	4753.0
7	5678.0
8	6484.0
9	7129.0
10	8029.0
11	8910.0
12	9412.0
13	10851.0
14	13552.0
15	11265.0
16	6715.0
17	5594.0
18	4975.0
19	4623.0
20	4449.0
21	4701.0
22	5523.0
23	7148.0
24	10058.0
25	14303.0
26	17766.0
27	19665.0
28	23032.0
29	27526.0
30	31863.0
31	38400.0
32	48058.0
33	61410.0
34	85246.0
35	135550.0
36	271174.0
37	421951.0
38	123557.0
39	311.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.926342704185586	16.95036688817568	12.04864764086242	25.07464276677631
2	17.272802849171487	19.464815991008216	37.148633774387385	26.113747385432912
3	19.473878524411862	24.04240104040206	28.748889916282227	27.734830518903852
4	13.587194484924705	15.25662268181183	34.70944044078015	36.446742392483316
5	14.427594263369306	36.99239346932042	33.137371256441334	15.442641010868943
6	34.33434598695812	34.43758477121501	16.36580853861909	14.862260703207786
7	29.905533745644853	31.293326965519352	20.021631056130328	18.779508232705467
8	28.478209974912406	33.428354035697275	18.61822694827912	19.475209041111196
9	28.001588236937287	14.000228791250596	18.184248786541755	39.81393418527037
10-11	26.87646967469371	24.515113352220105	26.37481406152157	22.233602911564617
12-13	27.194790744199825	22.114795306721504	26.93237923866411	23.758034710414556
14-15	24.412188502721367	23.094952028995095	24.402511498509043	28.09034796977449
16-17	24.46129057468436	26.157624573696648	24.804727706267638	24.57635714535136
18-19	24.53370800528753	24.95870571901266	26.916821163422927	23.59076511227688
20-21	25.49028150301224	23.944600659484287	26.806556264851285	23.758561572652187
22-23	25.29010854086728	23.583644443025523	26.729892935698235	24.39635408040897
24-25	24.665248129772845	24.145269441083606	26.741271774419378	24.44821065472417
26-27	24.434707709498692	24.154755332663772	26.82787152387477	24.582665433962767
28-29	24.510557432572877	24.32897138822531	26.769507778767004	24.39096340043481
30-31	24.797653428342123	24.170033981917776	26.64146432563837	24.39084826410173
32-33	24.476623839639434	24.204975083343754	26.624737596879633	24.693663480137182
34-35	24.699785571813486	24.359135116365625	26.67304374607738	24.26803556574351
36-37	24.863615063056518	24.212812052360906	26.527705856196082	24.39586702838649
38-39	24.945104067786914	24.294069243710002	26.346255801407438	24.414570887095646
40-41	24.901028294733482	24.298285288600958	26.325509158873533	24.475177257792026
42-43	24.81341608363003	24.389998131001498	26.21912244866739	24.57746333670108
44-45	24.740838250940477	24.421685741206428	26.13568371839946	24.701792289453632
46-47	24.9233971266564	24.477033439195463	25.83418392206046	24.765385512087672
48-49	25.065888028268326	24.767545971949858	25.35132521080178	24.815240788980038
50-51	24.96214435397964	25.03600984030621	25.263492922914832	24.73835288279932
52-53	25.161470572094252	24.93287234520228	25.299467668467713	24.60618941423575
54-55	24.913802192203068	24.82463606016735	25.48631461177146	24.775247135858123
56-57	24.87643899512142	24.875108593992174	25.344706932587247	24.90374547829916
58-59	24.97477342645714	24.746919431279622	25.398553255175855	24.879753887087386
60-61	25.046106054735244	24.782124986072564	25.55983310356526	24.611935855626935
62-63	24.857479451763197	24.875040910215713	25.55830803330291	24.70917160471818
64-65	24.889837732451614	24.644053399390227	25.55079470822007	24.915314159938085
66-67	24.926817879783776	24.847000979752448	25.471867535872704	24.75431360459107
68-69	24.8808925112289	24.86279855035443	25.391348425825406	24.86496051259127
70-71	24.806322464864664	24.68300207329914	25.543850243547794	24.966825218288403
72-73	25.01741103410567	24.987078917382895	25.238482941842822	24.75702710666861
74-75	24.881696283061515	25.06206205673523	25.201718314093874	24.85452334610938
76-77	24.920340302635346	24.834702594373585	25.181776428711345	25.06318067427972
78-79	24.848845296816048	24.8974423197245	25.234960452135258	25.01875193132419
80-81	24.900993441470305	25.043246251021493	25.06350156105579	24.99225874645241
82-83	24.96055843404346	24.95354142694664	25.135152212518875	24.950747926491033
84-85	24.834211552045932	24.991452832271975	25.063189177755813	25.11114643792628
86-87	24.69776882204087	25.089236808699493	25.130408957835794	25.082585411423842
88-89	24.802687113813864	24.914206727363315	25.10225680043477	25.180849358388052
90-91	24.727014891668986	24.90431012664753	25.13564864747116	25.233026334212326
92-93	24.74734894727758	25.07016098699939	25.12193994505511	25.06055012066792
94-95	24.733400531220248	24.815474624705566	25.140944297696173	25.310180546378014
96-97	24.60012975685056	24.88757676254995	25.199465409756638	25.31282807084285
98-99	24.68646114013587	24.87304648206323	25.059831344940797	25.380661032860104
100-101	24.60383630854491	24.94927501770095	25.17279418244897	25.274094491305167
102-103	24.582336679916214	24.79093971794528	25.286226446528886	25.34049715560962
104-105	24.608512351925373	24.951324877800918	25.012711231740802	25.427451538532907
106-107	24.600247802136046	24.866239386440313	25.062366197820058	25.47114661360358
108-109	24.587862472485916	24.766766379236373	25.1362483461616	25.509122802116114
110-111	24.61089697951974	24.943694161783725	25.081162542227297	25.364246316469234
112-113	24.569565275228136	24.927268736531246	25.10894724239764	25.394218745842977
114-115	24.380598687540278	25.05797794477081	25.125483094116806	25.4359402735721
116-117	24.40646547739165	24.97620540966935	25.114982244679556	25.502346868259444
118-119	24.426339798660642	24.783577317047133	25.181786407082495	25.60829647720973
120-121	24.210128314867248	24.891774028028017	25.288064981474058	25.610032675630684
122-123	24.215198919137134	24.946969417340295	25.19307654526911	25.64475511825346
124-125	24.247044505622615	24.917155022136896	25.130424919982215	25.705375552258275
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	31.0
1	36.0
2	86.5
3	126.0
4	90.0
5	77.5
6	149.5
7	318.5
8	516.5
9	605.0
10	547.0
11	484.5
12	529.5
13	623.5
14	748.5
15	885.5
16	1044.0
17	1162.5
18	1299.5
19	1472.5
20	1627.0
21	1843.0
22	2127.5
23	2479.0
24	2777.5
25	3188.5
26	3954.0
27	4776.0
28	5474.5
29	6402.5
30	7645.0
31	8787.5
32	10077.5
33	11471.5
34	13016.0
35	14821.0
36	17028.5
37	19566.5
38	22156.5
39	25076.5
40	27964.0
41	30663.0
42	33566.5
43	37934.0
44	42899.0
45	46727.0
46	50479.5
47	54598.0
48	59420.0
49	63359.0
50	64450.0
51	65493.0
52	66059.5
53	66553.0
54	67793.5
55	67220.0
56	63849.5
57	58576.5
58	54755.5
59	50977.5
60	45169.5
61	39436.0
62	33691.0
63	26639.5
64	20243.0
65	15834.0
66	12102.0
67	9204.0
68	7266.0
69	5663.0
70	4624.5
71	3834.0
72	3068.0
73	2605.5
74	2054.0
75	1483.0
76	1014.0
77	564.0
78	312.0
79	189.0
80	88.0
81	37.0
82	20.0
83	9.5
84	5.0
85	2.5
86	3.0
87	3.5
88	1.5
89	1.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11991037647069779
2	0.0018621689080308029
3	0.023809159609822408
4	0.05539952501391639
5	0.02932916030148515
6	0.02074988211805752
7	0.015229881426394781
8	0.006052048951100109
9	0.004588916237647336
10-11	0.00675036229161166
12-13	0.0045556632214325005
14-15	0.003890602897135785
16-17	0.00967662771851721
18-19	0.005686265772736916
20-21	0.011671808691407354
22-23	0.008911808345575985
24-25	0.01303518235621562
26-27	0.010175422961739745
28-29	0.013533977599438158
30-31	0.021514701490998742
32-33	0.014032772842660694
34-35	0.021448195458569068
36-37	0.016593255091203046
38-39	0.021547954507213574
40-41	0.01084048328603646
42-43	0.009576868669872701
44-45	0.017723857642507463
46-47	0.016792773188492063
48-49	0.020949400215346532
50-51	0.014265543956164544
52-53	0.021980243718006442
54-55	0.016593255091203046
56-57	0.021082412280205874
58-59	0.01649349604255854
60-61	0.019652532582967938
62-63	0.022013496734221278
64-65	0.018122893837085492
66-67	0.012104097902200218
68-69	0.02387566564225208
70-71	0.014764339199387081
72-73	0.017690604626292628
74-75	0.019220243372175073
76-77	0.013068435372430456
78-79	0.029561931414988998
80-81	0.020849641166702026
82-83	0.008845302313146314
84-85	0.013367712518363979
86-87	0.01193783282112604
88-89	0.01995180972890146
90-91	0.0132014474372898
92-93	0.007747952778056733
94-95	0.006883374356471003
96-97	0.0027600003458313686
98-99	0.001429879697237938
100-101	0.01147229059411834
102-103	0.0032920486052687406
104-105	0.0020949400215346534
106-107	2.660241297186861E-4
108-109	0.020084821793760803
110-111	2.992771459335219E-4
112-113	0.00951036263744303
114-115	0.002261205102608832
116-117	0.0086457842158573
118-119	0.009410603588798522
120-121	0.0037243378160616058
122-123	6.318073080818795E-4
124-125	0.0241749427881856
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1503623.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.07885406946845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.21301159861736	48.19105818305912
2	10.413470218848147	12.512587152698668
3	3.5195769230207903	6.343564450333044
4	1.7124496393157194	4.1152804792705195
5	0.9940225749088686	2.985986860985359
6	0.6833611829878812	2.463333407368094
7	0.456661190245565	1.9204976705567145
8	0.3361300127659704	1.615544478826824
9	0.26642475148847056	1.44058443886532
>10	1.3317593286633411	13.92531557227229
>50	0.05516847166594937	2.211592007439436
>100	0.017520572929966345	1.7956080104958183
>500	1.1088363549126717E-4	0.05216159454456253
>1k	3.326509064738015E-4	0.4268856932842359
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2700	0.17956628756011314	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2380	0.15828435718261827	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	6.650603242967153E-5	9.975904864450729E-5
14-15	0.0	0.0	0.0	6.650603242967153E-5	1.3301206485934305E-4
16-17	0.0	0.0	0.0	6.650603242967153E-5	2.3277111350385036E-4
18-19	0.0	0.0	0.0	6.650603242967153E-5	2.660241297186861E-4
20-21	0.0	0.0	0.0	6.650603242967153E-5	2.660241297186861E-4
22-23	0.0	0.0	0.0	1.3301206485934305E-4	3.3253016214835766E-4
24-25	0.0	0.0	0.0	1.995180972890146E-4	3.3253016214835766E-4
26-27	0.0	0.0	0.0	2.660241297186861E-4	3.990361945780292E-4
28-29	0.0	0.0	0.0	8.978314378005657E-4	3.990361945780292E-4
30-31	0.0	0.0	0.0	0.0036910847998467703	4.3228921079286497E-4
32-33	0.0	0.0	0.0	0.008679037232072136	4.655422270077007E-4
34-35	0.0	0.0	0.0	0.014764339199387081	5.320482594373722E-4
36-37	0.0	0.0	0.0	0.02457397898276363	5.320482594373722E-4
38-39	0.0	0.0	0.0	0.04173253534961889	5.320482594373722E-4
40-41	0.0	0.0	0.0	0.06148482698123133	5.320482594373722E-4
42-43	0.0	0.0	0.0	0.0794082027210278	5.985542918670438E-4
44-45	0.0	0.0	0.0	0.09945977149857377	5.985542918670438E-4
46-47	0.0	0.0	0.0	0.1225373647516698	5.985542918670438E-4
48-49	0.0	0.0	0.0	0.14864098248031588	7.315663567263868E-4
50-51	0.0	0.0	0.0	0.1772718294412895	7.315663567263868E-4
52-53	0.0	0.0	0.0	0.20653448371034494	8.978314378005657E-4
54-55	0.0	0.0	0.0	0.2377258129198609	9.97590486445073E-4
56-57	0.0	0.0	0.0	0.27300726312380164	9.97590486445073E-4
58-59	0.0	0.0	0.0	0.3161364251544436	9.97590486445073E-4
60-61	0.0	0.0	0.0	0.36202558753091696	9.97590486445073E-4
62-63	0.0	0.0	0.0	0.41100728041537005	9.97590486445073E-4
64-65	0.0	0.0	0.0	0.45952343107281546	9.97590486445073E-4
66-67	0.0	0.0	0.0	0.5145239198921538	9.97590486445073E-4
68-69	0.0	0.0	0.0	0.5779706748300604	9.97590486445073E-4
70-71	0.0	0.0	0.0	0.6502294790648986	0.0010973495350895804
72-73	0.0	0.0	0.0	0.7263456331806577	0.001562891762097281
74-75	0.0	0.0	0.0	0.80482275144767	0.0015961447783121169
76-77	0.0	0.0	0.0	0.8889528824712045	0.0016959038269566242
78-79	0.0	0.0	0.0	0.9856194006077321	0.0017291568431714599
80-81	0.0	0.0	0.0	1.097183270008506	0.0018289158918159672
82-83	0.0	0.0	0.0	1.2157302728143957	0.001995180972890146
84-85	0.0	0.0	0.0	1.3320493235338913	0.001995180972890146
86-87	0.0	0.0	0.0	1.4524917482640263	0.001995180972890146
88-89	0.0	0.0	0.0	1.5941163443230117	0.0020616870053198175
90-91	0.0	0.0	0.0	1.7612792568349913	0.0020949400215346534
92-93	0.0	0.0	0.0	1.9400807250221632	0.002128193037749489
94-95	0.0	0.0	0.0	2.1187824341606905	0.002261205102608832
96-97	0.0	0.0	0.0	2.303403180185459	0.0023942171674681753
98-99	0.0	0.0	0.0	2.5062798321121718	0.002527229232327518
100-101	0.0	0.0	0.0	2.740347813248401	0.002527229232327518
102-103	0.0	0.0	0.0	3.0037782077023296	0.002527229232327518
104-105	0.0	0.0	0.0	3.2665102888157467	0.002560482248542354
106-107	3.3253016214835764E-5	0.0	0.0	3.5450375526312117	0.0026269882809720254
108-109	6.650603242967153E-5	0.0	0.0	3.837065541029899	0.0027600003458313686
110-111	6.650603242967153E-5	0.0	0.0	4.161382208173192	0.0029262654269055473
112-113	6.650603242967153E-5	0.0	0.0	4.5098405650884565	0.002992771459335219
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2360	0.0	80.07552	1
GTATCAA	4620	0.0	58.711334	1
TCAACGC	6265	0.0	43.246502	4
CAACGCA	6295	0.0	42.918663	5
TATCAAC	6580	0.0	41.449066	2
ATCAACG	6535	0.0	41.36861	3
AACGCAG	6580	0.0	40.959747	6
GTACATG	7655	0.0	37.30292	1
TACATGG	7860	0.0	35.83552	2
ACGCAGA	7830	0.0	34.190605	7
ACATGGG	8050	0.0	34.174862	3
CGCAGAG	8160	0.0	32.73499	8
CATGGGG	7110	0.0	29.982933	4
GCAGAGT	8815	0.0	29.693233	9
AGAGTAC	8315	0.0	26.542755	10-11
CAGAGTA	8655	0.0	26.187393	10-11
GTATAGA	510	0.0	25.716095	1
GAGTACT	5005	0.0	25.434454	12-13
ATGGGGG	3720	0.0	24.955584	5
GTATAGG	435	0.0	24.668104	1
>>END_MODULE