FastQCFastQC Report
Mon 15 Aug 2016
SRR2921515_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921515_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1653485
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17600.067.300191
GTATCAA34650.059.7794531
TCAACGC44400.045.5590554
CAACGCA45900.044.0701985
AACGCAG48200.041.964726
ATCAACG48700.041.658553
TATCAAC51300.039.7791522
GTACATG83750.039.017861
TACATGG88150.036.7834932
ACATGGG88900.036.0047073
ACGCAGA56900.035.440557
CGCAGAG60550.033.5006568
CATGGGG70800.032.9406054
GCAGAGT67900.028.9982139
GTATAGG5350.027.8139171
ATGGGGG39300.027.249515
AGAGTAC60600.026.11091410-11
CAGAGTA64200.024.83206610-11
GTATATA5100.023.3418751
TTAGCGC2352.912675E-722.7852294
Produced by FastQC (version 0.11.5)