##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921515_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1653485
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.522204918702013	31.0	30.0	34.0	25.0	34.0
2	29.620758579606104	31.0	30.0	34.0	25.0	34.0
3	29.710186061560886	31.0	30.0	34.0	25.0	34.0
4	33.09368817981415	37.0	35.0	37.0	26.0	37.0
5	33.105042380184884	37.0	35.0	37.0	26.0	37.0
6	33.191490699945874	37.0	35.0	37.0	26.0	37.0
7	33.0763750502726	37.0	35.0	37.0	26.0	37.0
8	33.0449057596531	37.0	35.0	37.0	25.0	37.0
9	34.497384010136166	39.0	35.0	39.0	25.0	39.0
10-11	34.362147524773434	39.0	35.0	39.0	25.0	39.0
12-13	34.208139172717026	39.0	35.0	39.0	23.5	39.0
14-15	35.33654372431561	40.0	36.0	41.0	24.0	41.0
16-17	35.25634523445934	40.0	35.0	41.0	23.5	41.0
18-19	35.26884610383523	40.0	35.0	41.0	23.0	41.0
20-21	35.288475250758246	40.0	35.0	41.0	23.5	41.0
22-23	35.25473227758341	40.0	35.0	41.0	24.0	41.0
24-25	35.21532188075489	40.0	35.0	41.0	24.0	41.0
26-27	35.13178075398325	40.0	35.0	41.0	24.0	41.0
28-29	35.0872339331775	40.0	35.0	41.0	24.0	41.0
30-31	34.98740236530722	40.0	35.0	41.0	24.0	41.0
32-33	34.871545553784884	39.5	34.5	41.0	23.0	41.0
34-35	34.710241096834864	39.0	34.0	41.0	22.0	41.0
36-37	34.57051863185937	39.0	34.0	41.0	21.5	41.0
38-39	34.43881135903864	39.0	34.0	41.0	20.0	41.0
40-41	34.27776726126938	39.0	33.5	41.0	18.5	41.0
42-43	34.07863179889748	39.0	33.0	41.0	17.0	41.0
44-45	33.88483717723475	39.0	33.0	41.0	15.0	41.0
46-47	33.62701536451797	38.0	33.0	41.0	13.5	41.0
48-49	33.469447560758034	38.0	33.0	40.0	10.5	41.0
50-51	33.266751437116156	38.0	33.0	40.0	8.5	41.0
52-53	33.0703054457706	38.0	32.5	40.0	8.0	41.0
54-55	32.86264012071473	37.5	32.0	40.0	7.0	41.0
56-57	32.6161074941714	37.0	32.0	40.0	4.0	41.0
58-59	32.36646325790679	36.5	32.0	40.0	2.0	41.0
60-61	32.060556642485416	36.0	31.0	40.0	2.0	41.0
62-63	31.745724333755675	35.5	31.0	39.0	2.0	41.0
64-65	31.43448957807298	35.0	31.0	39.0	2.0	41.0
66-67	31.39642361436602	35.0	31.0	39.0	2.0	41.0
68-69	31.1584810264381	35.0	31.0	38.0	2.0	40.0
70-71	30.863641944136173	35.0	31.0	37.0	2.0	40.0
72-73	30.54662183206984	35.0	30.5	37.0	2.0	39.0
74-75	30.2511552871662	35.0	30.0	36.0	2.0	39.0
76-77	29.97962606252854	35.0	30.0	36.0	2.0	38.5
78-79	29.711136478407724	35.0	30.0	35.5	2.0	37.0
80-81	29.4774452141991	35.0	30.0	35.0	2.0	37.0
82-83	29.281859224607423	35.0	30.0	35.0	2.0	36.5
84-85	29.085036453309222	34.0	30.0	35.0	2.0	36.0
86-87	28.92866400360451	34.0	29.0	35.0	2.0	36.0
88-89	28.801905369567912	34.0	29.0	35.0	2.0	35.5
90-91	28.69115625481937	34.0	29.0	35.0	2.0	35.0
92-93	28.59167697318089	34.0	29.0	35.0	2.0	35.0
94-95	28.51934127010526	34.0	29.0	35.0	2.0	35.0
96-97	28.41799260350109	34.0	29.0	35.0	2.0	35.0
98-99	28.352813602784423	34.0	29.0	35.0	2.0	35.0
100-101	28.286475535006367	34.0	29.0	35.0	2.0	35.0
102-103	27.31056858695422	32.5	26.0	34.5	2.0	35.0
104-105	28.126165039295792	34.0	29.0	35.0	2.0	35.0
106-107	28.45415682633952	34.0	29.0	35.0	2.0	35.0
108-109	28.491452900993963	34.0	29.0	35.0	2.0	35.0
110-111	28.42901780179439	34.0	29.0	35.0	2.0	35.0
112-113	28.35099592678494	34.0	29.0	35.0	2.0	35.0
114-115	28.22785389646716	34.0	29.0	35.0	2.0	35.0
116-117	28.16328149333075	34.0	29.0	35.0	2.0	35.0
118-119	27.999208943534413	34.0	29.0	35.0	2.0	35.0
120-121	27.819760082492433	34.0	29.0	35.0	2.0	35.0
122-123	27.686247229336825	34.0	29.0	35.0	2.0	35.0
124-125	26.835610543790843	33.5	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	69073.0
3	11981.0
4	7947.0
5	6802.0
6	7058.0
7	8122.0
8	8984.0
9	9127.0
10	9919.0
11	10385.0
12	10434.0
13	10632.0
14	12246.0
15	10411.0
16	7517.0
17	6710.0
18	6017.0
19	5689.0
20	5711.0
21	5888.0
22	6951.0
23	9521.0
24	13576.0
25	18539.0
26	21779.0
27	23450.0
28	27337.0
29	32416.0
30	36682.0
31	43612.0
32	54587.0
33	71171.0
34	98681.0
35	154326.0
36	289995.0
37	409306.0
38	110759.0
39	144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.24610235691702	16.95736398934735	11.994576876585828	24.8019567771498
2	16.985937407367526	19.158059282363745	38.17571041924021	25.680292891028518
3	19.438682329938977	23.917040069835014	28.63181086471644	28.012466735509566
4	13.358947879782235	15.095643167709943	35.324177675658376	36.221231276849444
5	14.517960585023518	37.01178214377543	33.316756658650576	15.153500612550477
6	33.88982504757266	34.4273582879288	17.088643974986915	14.594172689511629
7	30.089657421406823	30.97485732194208	20.401767374717682	18.533717881933416
8	28.001749614020145	33.463364582778766	19.19088216361674	19.34400363958435
9	27.72956989182263	14.588276288455232	18.694866263446553	38.98728755627558
10-11	26.55671434063845	24.77303162235995	26.70396813897274	21.96628589802886
12-13	26.721424092359825	22.507201620882718	26.887160439378277	23.88421384737918
14-15	24.16833036891841	23.718828269628663	24.17589265188089	27.936948709572036
16-17	24.396521865390927	26.678030549383735	24.642695629946807	24.282751955278535
18-19	24.46927080213399	25.75941849566673	26.246778715043877	23.5245319871554
20-21	25.511480435483787	24.726370296767275	25.949511862884695	23.812637404864244
22-23	25.28657107389233	24.35906318556723	25.80024633902749	24.55411940151295
24-25	24.733924578427725	24.887152068601377	25.872328579771185	24.506594773199712
26-27	24.418124116983268	24.882299731048608	25.996194141155616	24.70338201081251
28-29	24.45949858459014	24.899263418106656	26.04823073011067	24.593007267192533
30-31	24.79264159003421	24.93604381694373	25.812219321907143	24.45909527111492
32-33	24.398927365404056	24.950100343919594	25.8133636377114	24.83760865296495
34-35	24.707772476207214	25.004348511326853	25.912529429968394	24.375349582497538
36-37	24.770816233359767	24.818043171048345	25.772928890027796	24.638211705564096
38-39	24.80937314392029	24.979750482372708	25.739353231768554	24.47152314193845
40-41	24.83456621450771	24.937851716024774	25.836048334226312	24.3915337352412
42-43	24.744887539329238	24.933201940872788	25.746033945607273	24.5758765741907
44-45	24.622839317537494	24.970031073283934	25.738875492044087	24.66825411713448
46-47	24.71918985797461	25.072362206201575	25.540121915869214	24.668326019954602
48-49	24.903256996793434	25.181498072703302	25.256072852753082	24.65917207775018
50-51	24.779213533966065	25.403929335019086	25.18240920836355	24.634447922651294
52-53	24.904979089650965	25.22370230876153	25.316469101033505	24.554849500554
54-55	24.778035799857694	25.17951963522481	25.493694123227794	24.548750441689698
56-57	24.707068946948713	25.24546551321472	25.291115927244917	24.75634961259165
58-59	24.760945906372598	25.07693173375025	25.336254903906426	24.825867455970727
60-61	24.775235321555204	25.25474136861891	25.477040759223957	24.492982550601923
62-63	24.60856683291944	25.398527170031297	25.36037988763946	24.632526109409795
64-65	24.69865795586861	25.03650591158294	25.46489639339442	24.79993973915403
66-67	24.627768182051927	25.257889753680573	25.413370761705696	24.700971302561804
68-69	24.654281560816138	25.29145369620334	25.349711162747646	24.704553580232872
70-71	24.519071064272907	25.028090618489767	25.624059486157996	24.828778831079326
72-73	24.788691409753394	25.285916561880757	25.312565506948538	24.612826521417315
74-75	24.517222154494355	25.489236361414314	25.28803922179572	24.70550226229561
76-77	24.628451904360755	25.156104193480573	25.374890538768557	24.840553363390114
78-79	24.615627067283448	25.22304353126118	25.344460277758202	24.816869123697167
80-81	24.571704763399794	25.422805532291104	25.20176553729989	24.803724167009207
82-83	24.720790397494305	25.305991101465136	25.19841143822547	24.77480706281509
84-85	24.556923129036083	25.378868529867916	25.17278485759842	24.89142348349758
86-87	24.34561750939671	25.48931248858301	25.32983756724682	24.83523243477346
88-89	24.507434680010235	25.257889423161263	25.29515204784134	24.939523848987164
90-91	24.464692799807416	25.298054863506568	25.294123339762887	24.94312899692313
92-93	24.56043356138717	25.438583491386208	25.228384007539358	24.772598939687267
94-95	24.470839504238757	25.172092274908508	25.38712255909628	24.96994566175646
96-97	24.485125027671696	25.212814988562364	25.337352735173113	24.964707248592827
98-99	24.462716791325082	25.249226101693683	25.278016691283828	25.010040415697404
100-101	24.398767080119832	25.310694721077887	25.32608807417358	24.9644501246287
102-103	24.501170168402037	25.08632997146174	25.49083113558198	24.921668724554245
104-105	24.29224199977017	25.349675515153713	25.318073775697204	25.040008709378913
106-107	24.29864995067581	25.32683701606499	25.33197810260036	25.04253493065884
108-109	24.326022013196788	25.25335983417217	25.294066767839222	25.126551384791817
110-111	24.363462396833672	25.361104827754723	25.298234302932503	24.977198472479103
112-113	24.352277861411626	25.28963553854519	25.403705520418583	24.954381079624596
114-115	24.110098423994334	25.508193330297086	25.413960808222303	24.967747437486278
116-117	24.139561974218765	25.576956923815743	25.287639268332068	24.995841833633428
118-119	24.311161429465642	25.189307652785164	25.309041086755208	25.190489830993982
120-121	23.934341359141513	25.351365556737715	25.52704074851166	25.187252335609113
122-123	24.021624813181543	25.415628534275537	25.41075862586308	25.151988026679838
124-125	23.950427703489154	25.465072922938376	25.396064589622686	25.188434783949788
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	160.0
1	174.0
2	181.0
3	180.0
4	171.0
5	149.5
6	160.5
7	196.5
8	272.0
9	340.0
10	344.5
11	380.0
12	447.0
13	507.5
14	560.5
15	630.0
16	728.0
17	817.0
18	904.5
19	972.5
20	1097.0
21	1277.0
22	1533.5
23	1923.5
24	2285.0
25	2848.0
26	3898.5
27	5010.0
28	6032.0
29	7433.5
30	9311.0
31	11217.5
32	13364.0
33	15668.5
34	18220.0
35	20817.5
36	23928.5
37	27252.5
38	29637.5
39	32216.0
40	34804.5
41	37018.0
42	39407.5
43	43632.0
44	48306.5
45	51861.5
46	55559.0
47	59325.5
48	63618.5
49	67999.5
50	68804.0
51	68642.5
52	68906.5
53	69087.0
54	69841.0
55	68967.5
56	65441.5
57	60452.0
58	57169.5
59	53170.0
60	47191.0
61	41893.0
62	36505.5
63	29043.0
64	22115.0
65	17504.5
66	13683.5
67	10428.0
68	8197.0
69	6548.0
70	5598.5
71	4647.5
72	3610.0
73	3207.0
74	2648.5
75	1996.5
76	1355.0
77	767.0
78	470.0
79	307.0
80	180.0
81	99.0
82	54.0
83	32.0
84	27.0
85	17.5
86	10.5
87	13.0
88	17.0
89	12.5
90	12.5
91	12.0
92	8.0
93	7.0
94	8.0
95	9.5
96	9.0
97	6.5
98	6.5
99	7.5
100	21.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03404929588112381
2	0.027033810406505047
3	0.026973332083447987
4	0.03132777134355619
5	0.03386786091195263
6	0.045358742292793706
7	0.03858517011040318
8	0.033202599358324994
9	0.02775955028318975
10-11	0.03365618678125293
12-13	0.03377714342736705
14-15	0.03296068606609676
16-17	0.035531014796021734
18-19	0.04874552838398897
20-21	0.05914779994980299
22-23	0.05349307674396805
24-25	0.06289745597934061
26-27	0.04656830875393487
28-29	0.04738476611520516
30-31	0.047838353538133094
32-33	0.05554933972790803
34-35	0.03752679945690466
36-37	0.049985334006658666
38-39	0.0590570824652174
40-41	0.047445244438262216
42-43	0.056214601281535666
44-45	0.05684962367363478
46-47	0.06253458604099825
48-49	0.04726380946909104
50-51	0.04919911580691691
52-53	0.05769632019643359
54-55	0.04481443738528018
56-57	0.0427581744013402
58-59	0.04360487092413901
60-61	0.03229542451246912
62-63	0.04115549884032815
64-65	0.04055071560975757
66-67	0.03326307768138205
68-69	0.028908638421273853
70-71	0.0482617017995325
72-73	0.03093466224368531
74-75	0.03801062604136113
76-77	0.03039035733617178
78-79	0.03026940069005767
80-81	0.023616785153781256
82-83	0.017629431171132488
84-85	0.01651058219457691
86-87	0.014938145795093392
88-89	0.02140932636219863
90-91	0.011007054796384606
92-93	0.017750387817246603
94-95	0.013879775141594875
96-97	0.010341793242756964
98-99	0.010099879950528731
100-101	0.010523228211928139
102-103	0.005473288236663774
104-105	0.005745440690420536
106-107	0.008346008581874042
108-109	0.012337577903639887
110-111	0.0052616141059640695
112-113	0.006894528828504643
114-115	0.007741225351303459
116-117	0.006773572182390527
118-119	0.24115731319001987
120-121	0.3533143632993344
122-123	0.028817920936688265
124-125	0.003719416868009084
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1653485.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.11915849474112
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.24800922170988	48.84561943782164
2	9.761930755521204	11.737581246117344
3	3.313024920356052	5.9752881085173755
4	1.651466089680737	3.971390063768263
5	0.9490150226192144	2.852699227936744
6	0.6337003461947668	2.2858518930633296
7	0.432602404434439	1.8205384762180092
8	0.3316525253091241	1.5950936587392297
9	0.2403872152494328	1.300668938432109
>10	1.3527119612236067	14.386228116153847
>50	0.06402361149018616	2.6372608266560804
>100	0.020766895794103325	2.187499884278506
>500	4.0516023846608054E-4	0.15765645980242812
>1k	3.0387017884956046E-4	0.2466236624951077
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	6.047832305705828E-5	0.0
3	0.0	0.0	0.0	6.047832305705828E-5	0.0
4	0.0	0.0	0.0	1.2095664611411655E-4	0.0
5	0.0	0.0	0.0	1.2095664611411655E-4	0.0
6	0.0	0.0	0.0	1.2095664611411655E-4	0.0
7	0.0	0.0	0.0	1.2095664611411655E-4	0.0
8	0.0	0.0	0.0	1.2095664611411655E-4	0.0
9	0.0	0.0	0.0	1.8143496917117483E-4	0.0
10-11	0.0	0.0	0.0	1.8143496917117483E-4	0.0
12-13	0.0	0.0	0.0	1.8143496917117483E-4	1.511958076426457E-4
14-15	0.0	0.0	0.0	1.8143496917117483E-4	2.419132922282331E-4
16-17	0.0	0.0	0.0	1.8143496917117483E-4	3.0239161528529136E-4
18-19	0.0	0.0	0.0	3.0239161528529136E-4	3.931090998708788E-4
20-21	0.0	0.0	0.0	3.0239161528529136E-4	5.140657459849953E-4
22-23	0.0	0.0	0.0	3.326307768138205E-4	6.65261553627641E-4
24-25	0.0	0.0	0.0	3.931090998708788E-4	7.257398766846993E-4
26-27	0.0	0.0	0.0	5.140657459849953E-4	7.862181997417575E-4
28-29	0.0	0.0	0.0	9.978923304414615E-4	7.862181997417575E-4
30-31	0.0	0.0	0.0	0.003265829445081147	8.466965227988158E-4
32-33	0.0	0.0	0.0	0.008557682712573745	9.071748458558741E-4
34-35	0.0	0.0	0.0	0.015240537410378684	9.071748458558741E-4
36-37	0.0	0.0	0.0	0.026338309691348878	9.071748458558741E-4
38-39	0.0	0.0	0.0	0.04154860794019903	9.071748458558741E-4
40-41	0.0	0.0	0.0	0.05878493001146064	9.071748458558741E-4
42-43	0.0	0.0	0.0	0.07668651363634989	9.071748458558741E-4
44-45	0.0	0.0	0.0	0.09782368754479176	9.071748458558741E-4
46-47	0.0	0.0	0.0	0.12125903772940183	9.374140073844033E-4
48-49	0.0	0.0	0.0	0.1456015627598678	9.978923304414615E-4
50-51	0.0	0.0	0.0	0.17094198012077522	0.0010281314919699906
52-53	0.0	0.0	0.0	0.19876200872702202	0.001088609815027049
54-55	0.0	0.0	0.0	0.22773112547135294	0.001088609815027049
56-57	0.0	0.0	0.0	0.2615385080602485	0.0011188489765555781
58-59	0.0	0.0	0.0	0.30239161528529135	0.0012700447841982238
60-61	0.0	0.0	0.0	0.3459057687248448	0.0013910014303123402
62-63	0.0	0.0	0.0	0.38960135713356936	0.0014514797533693986
64-65	0.0	0.0	0.0	0.43598823091833305	0.0014514797533693986
66-67	0.0	0.0	0.0	0.49250522381515405	0.0014514797533693986
68-69	0.0	0.0	0.0	0.5540116783641824	0.0014514797533693986
70-71	0.0	0.0	0.0	0.6217474001880876	0.0014514797533693986
72-73	0.0	0.0	0.0	0.692295364034146	0.0014514797533693986
74-75	0.0	0.0	0.0	0.764415764279688	0.0014817189148979277
76-77	0.0	0.0	0.0	0.8498413955977828	0.0015119580764264568
78-79	0.0	0.0	0.0	0.9443085362129079	0.0015119580764264568
80-81	0.0	0.0	0.0	1.0482405343864625	0.0015119580764264568
82-83	0.0	0.0	0.0	1.1565572109816538	0.0015119580764264568
84-85	0.0	0.0	0.0	1.2701052625212808	0.0015119580764264568
86-87	0.0	0.0	0.0	1.3903059295971842	0.0015119580764264568
88-89	0.0	0.0	0.0	1.530766834897202	0.0015119580764264568
90-91	6.047832305705828E-5	0.0	0.0	1.688615258076124	0.0015119580764264568
92-93	6.047832305705828E-5	0.0	0.0	1.8626718718343378	0.0015119580764264568
94-95	6.047832305705828E-5	0.0	0.0	2.0334324169859417	0.001572436399483515
96-97	6.047832305705828E-5	0.0	0.0	2.2129320798192906	0.0016631538840691023
98-99	6.047832305705828E-5	0.0	0.0	2.4120871976461835	0.0017538713686546898
100-101	6.047832305705828E-5	0.0	0.0	2.646077829553942	0.0017538713686546898
102-103	6.047832305705828E-5	0.0	0.0	2.898151480055761	0.0018748280147688067
104-105	6.047832305705828E-5	0.0	0.0	3.159599270631424	0.0019353063378258649
106-107	9.071748458558742E-5	0.0	0.0	3.4230730850294986	0.0019353063378258649
108-109	1.2095664611411655E-4	0.0	0.0	3.7069583334593297	0.0020260238224114524
110-111	1.2095664611411655E-4	0.0	0.0	4.020447721025591	0.0021167413069970394
112-113	1.2095664611411655E-4	0.0	0.0	4.360759244867658	0.0021469804685255688
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1760	0.0	67.30019	1
GTATCAA	3465	0.0	59.779453	1
TCAACGC	4440	0.0	45.559055	4
CAACGCA	4590	0.0	44.070198	5
AACGCAG	4820	0.0	41.96472	6
ATCAACG	4870	0.0	41.65855	3
TATCAAC	5130	0.0	39.779152	2
GTACATG	8375	0.0	39.01786	1
TACATGG	8815	0.0	36.783493	2
ACATGGG	8890	0.0	36.004707	3
ACGCAGA	5690	0.0	35.44055	7
CGCAGAG	6055	0.0	33.500656	8
CATGGGG	7080	0.0	32.940605	4
GCAGAGT	6790	0.0	28.998213	9
GTATAGG	535	0.0	27.813917	1
ATGGGGG	3930	0.0	27.24951	5
AGAGTAC	6060	0.0	26.110914	10-11
CAGAGTA	6420	0.0	24.832066	10-11
GTATATA	510	0.0	23.341875	1
TTAGCGC	235	2.912675E-7	22.785229	4
>>END_MODULE