FastQCFastQC Report
Mon 15 Aug 2016
SRR2921515_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921515_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1653485
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29530.17859248798749308No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27730.16770638983722258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26850.081.716191
GTATCAA51300.056.948631
TCAACGC65800.043.6121444
CAACGCA67300.042.2670565
ATCAACG69100.041.5293663
AACGCAG69700.040.801796
TATCAAC71950.040.0498472
GTACATG81900.035.743921
ACGCAGA82550.034.5214777
TACATGG85450.033.8618352
ACATGGG84950.033.150043
CGCAGAG87750.032.4747778
GTGGTAT8600.030.504141
CATGGGG69800.030.365534
GCAGAGT93850.029.6033179
CAGAGTA88600.027.76554110-11
GAGTACT49950.026.85932712-13
AGAGTAC85300.026.25912910-11
GTACTTT54600.024.95399514-15
ATGGGGG37500.024.1213445
Produced by FastQC (version 0.11.5)