FastQCFastQC Report
Mon 15 Aug 2016
SRR2921514_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921514_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1591517
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21570.13553106878531615No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16250.10210384180627666No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20400.075.5733261
GTATCAA40000.058.0368081
TCAACGC51900.043.5663534
CAACGCA52900.042.741455
AACGCAG55400.041.0210386
ATCAACG56000.040.5904663
GTACATG93550.040.5316731
TATCAAC59500.039.102852
TACATGG97800.038.1484872
ACATGGG96350.037.6727033
CATGGGG75900.034.4941834
ACGCAGA66150.034.1727147
CGCAGAG69900.032.3333178
GCAGAGT75650.029.5631039
ATGGGGG39900.028.482745
CATGGGT17400.028.0413934
CAGAGTA73150.025.53289610-11
AGAGTAC70050.025.09193210-11
ATGGGGA29800.024.3593045
TATAGGG3002.237357E-1023.8017372
Produced by FastQC (version 0.11.5)