FastQCFastQC Report
Mon 15 Aug 2016
SRR2921514_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921514_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1591517
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35590.22362312183910066No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30670.1927092201968311No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16900.10618799547852772No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25750.084.021571
GTATCAA54950.063.1272281
GTATACG602.1716434E-549.6683961
TCAACGC73450.047.0986484
CAACGCA75150.046.0057225
ATCAACG77300.044.8327033
AACGCAG79050.043.729126
TATCAAC80100.043.4885332
ACGCAGA91450.037.601027
CGCAGAG96000.036.00488
GTACATG91700.033.863341
GCAGAGT101800.033.544379
TACATGG95950.032.5190432
GTGGTAT9850.032.0701561
ACATGGG98550.030.81523
TGGTATC9550.030.5523172
CATGGGG77550.028.1011034
AGAGTAC95600.027.94156810-11
CAGAGTA100600.027.52859310-11
GAGTACT53900.026.15781212-13
Produced by FastQC (version 0.11.5)