FastQCFastQC Report
Mon 15 Aug 2016
SRR2921513_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921513_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1691896
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21150.070.079031
GTATCAA39000.054.33561
TCAACGC49500.041.467464
CAACGCA50450.040.4495435
AACGCAG53300.038.3948826
ATCAACG54100.038.0526773
TATCAAC54750.037.925812
GTACATG91400.036.7309381
TACATGG93850.035.755132
ACATGGG95050.034.803993
ACGCAGA62250.032.874667
CATGGGG79900.032.7641874
CGCAGAG68000.030.0939258
ATGGGGG42700.028.5631895
GCAGAGT74350.027.2836679
CAGAGTA70900.024.87682310-11
AGAGTAC67500.024.05887610-11
ATGGGGA31350.023.1527715
TACCGTA1802.180738E-523.1349057
GTATAGG4950.022.8479631
Produced by FastQC (version 0.11.5)