##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921513_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1691896
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.74071219507582	31.0	30.0	34.0	25.0	34.0
2	29.835446741407274	31.0	30.0	34.0	25.0	34.0
3	29.909239693219913	31.0	30.0	34.0	25.0	34.0
4	33.28330346546123	37.0	35.0	37.0	26.0	37.0
5	33.30521734196428	37.0	35.0	37.0	27.0	37.0
6	33.25372717944838	37.0	35.0	37.0	26.0	37.0
7	33.15539430319594	37.0	35.0	37.0	26.0	37.0
8	33.10164040815747	37.0	35.0	37.0	26.0	37.0
9	34.552156279109354	39.0	35.0	39.0	26.0	39.0
10-11	34.436987852681256	39.0	35.0	39.0	25.0	39.0
12-13	34.296359823535255	39.0	35.0	39.0	24.5	39.0
14-15	35.4347947509776	40.0	36.0	41.0	25.0	41.0
16-17	35.35241971137705	40.0	36.0	41.0	24.0	41.0
18-19	35.358983649113185	40.0	36.0	41.0	24.0	41.0
20-21	35.38493057492896	40.0	36.0	41.0	24.0	41.0
22-23	35.34146838812788	40.0	35.0	41.0	24.0	41.0
24-25	35.288753564048854	40.0	35.0	41.0	24.0	41.0
26-27	35.210570566985204	40.0	35.0	41.0	24.0	41.0
28-29	35.16233355950957	40.0	35.0	41.0	24.0	41.0
30-31	35.05950188427657	40.0	35.0	41.0	24.0	41.0
32-33	34.951756195416266	40.0	35.0	41.0	23.5	41.0
34-35	34.78576342753928	39.5	34.5	41.0	22.5	41.0
36-37	34.635959302463036	39.0	34.0	41.0	21.5	41.0
38-39	34.50645547953302	39.0	34.0	41.0	20.5	41.0
40-41	34.35176246057678	39.0	34.0	41.0	19.0	41.0
42-43	34.1508819099992	39.0	33.0	41.0	17.0	41.0
44-45	33.94603716776918	39.0	33.0	41.0	15.5	41.0
46-47	33.692470163650725	38.0	33.0	41.0	14.5	41.0
48-49	33.53663109316412	38.0	33.0	40.0	11.5	41.0
50-51	33.33393601025122	38.0	33.0	40.0	9.0	41.0
52-53	33.13108873122225	38.0	33.0	40.0	8.0	41.0
54-55	32.92386204589407	37.5	32.0	40.0	7.5	41.0
56-57	32.674943968187165	37.0	32.0	40.0	6.5	41.0
58-59	32.42358572867363	36.5	32.0	40.0	2.0	41.0
60-61	32.10824571959506	36.0	31.5	40.0	2.0	41.0
62-63	31.79274553518656	35.5	31.0	39.0	2.0	41.0
64-65	31.480845453857683	35.0	31.0	39.0	2.0	41.0
66-67	31.434866268375835	35.0	31.0	39.0	2.0	40.5
68-69	31.192416377838825	35.0	31.0	38.0	2.0	40.0
70-71	30.895283752665648	35.0	31.0	37.0	2.0	40.0
72-73	30.57392505213086	35.0	31.0	37.0	2.0	39.0
74-75	30.280238265236164	35.0	30.5	36.0	2.0	39.0
76-77	30.01171437251462	35.0	30.0	36.0	2.0	38.5
78-79	29.749100713046193	35.0	30.0	35.0	2.0	37.0
80-81	29.518460650063595	35.0	30.0	35.0	2.0	37.0
82-83	29.335119298112886	35.0	30.0	35.0	2.0	36.0
84-85	29.14502280282003	34.5	30.0	35.0	2.0	36.0
86-87	29.00339559878385	34.0	30.0	35.0	2.0	36.0
88-89	28.883260850548734	34.0	29.5	35.0	2.0	35.0
90-91	28.774211890092538	34.0	29.0	35.0	2.0	35.0
92-93	28.67428523975469	34.0	29.0	35.0	2.0	35.0
94-95	28.61409950729832	34.0	29.0	35.0	2.0	35.0
96-97	28.515278421368688	34.0	29.0	35.0	2.0	35.0
98-99	28.45202926184588	34.0	29.0	35.0	2.0	35.0
100-101	28.386201043090118	34.0	29.0	35.0	2.0	35.0
102-103	27.404682380004445	32.5	26.5	34.5	2.0	35.0
104-105	28.23255891615087	34.0	29.0	35.0	2.0	35.0
106-107	28.558075378155632	34.0	29.5	35.0	2.0	35.0
108-109	28.59787067290188	35.0	30.0	35.0	2.0	35.0
110-111	28.534328055625167	35.0	30.0	35.0	2.0	35.0
112-113	28.464265829578178	35.0	30.0	35.0	2.0	35.0
114-115	28.340821480752957	34.0	29.0	35.0	2.0	35.0
116-117	28.2750426149125	34.0	29.0	35.0	2.0	35.0
118-119	28.115953344650023	34.0	29.0	35.0	2.0	35.0
120-121	27.941789566261757	34.0	29.0	35.0	2.0	35.0
122-123	27.811692621768714	34.0	29.0	35.0	2.0	35.0
124-125	26.953843498654763	33.5	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	71167.0
3	12154.0
4	7934.0
5	6739.0
6	7351.0
7	8164.0
8	8949.0
9	9295.0
10	9840.0
11	10254.0
12	10547.0
13	10971.0
14	11982.0
15	10302.0
16	7484.0
17	6574.0
18	6096.0
19	5666.0
20	5445.0
21	5833.0
22	6952.0
23	9221.0
24	13381.0
25	18225.0
26	21782.0
27	23193.0
28	27426.0
29	32000.0
30	36972.0
31	44152.0
32	55386.0
33	71654.0
34	100601.0
35	157299.0
36	299959.0
37	428620.0
38	112215.0
39	111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.40469122210995	16.595765814930154	11.990511603596934	25.00903135936296
2	16.93895769901564	18.876939903632977	38.2921753525082	25.891927044843182
3	19.562100672859025	23.700588670357014	28.44087194394832	28.296438712835638
4	13.711015330767365	14.758923239740565	34.865877956922496	36.664183472569576
5	14.748581287300702	36.82297043377902	33.020382872096505	15.408065406823773
6	34.28200242917948	33.86996439016833	17.065518069239893	14.782515111412295
7	30.390603889542252	30.85582109832178	20.117214869685377	18.6363601424506
8	28.350635544366305	33.24484156030901	18.996207664372974	19.408315230951704
9	28.027709687985176	14.658264130060767	18.32405696129938	38.98996922065468
10-11	26.845044931586386	24.536870744196676	26.446858573038917	22.17122575117802
12-13	27.021956548088887	22.117046405269335	26.657354275865536	24.20364277077624
14-15	24.4083556999263	23.340523950312313	23.97422848187914	28.27689186788224
16-17	24.683763786692698	26.38099002548101	24.39202854440729	24.543217643419002
18-19	24.763634288572987	25.478356537980396	25.943119663717162	23.814889509729454
20-21	25.819754540852298	24.47592129977905	25.6414441779153	24.062879981453356
22-23	25.566998238012857	24.02535558343537	25.651741570093467	24.755904608458305
24-25	25.033948386623628	24.462060016489218	25.614649142832807	24.889342454054347
26-27	24.758158795900158	24.558494555874944	25.709794103089784	24.973552545135117
28-29	24.728906124570575	24.73266115357448	25.705509337160503	24.832923384694443
30-31	25.09882013095201	24.59961178597669	25.560833255720095	24.7407348273512
32-33	24.76576540880094	24.683059588996134	25.488472931807472	25.062702070395453
34-35	25.008440637337596	24.746943484094057	25.578176266524284	24.666439612044062
36-37	25.132596623006908	24.542158008930645	25.51085698606353	24.81438838199891
38-39	25.141384525990357	24.66455828147386	25.42957978670712	24.764477405828664
40-41	25.126302832902986	24.611068288239224	25.53923555703785	24.723393321819945
42-43	24.99969691064719	24.645718116256795	25.432478936768465	24.922106036327552
44-45	24.903188109583727	24.662784728678318	25.44053254227906	24.993494619458893
46-47	24.93016739126192	24.715538945688774	25.37441692802761	24.979876735021698
48-49	25.170689534865843	24.87614438566551	25.00567683885229	24.947489240616363
50-51	25.072810180238093	25.103235226112197	24.973256118568386	24.850698475081327
52-53	25.198159881646692	24.894913534131405	25.043594434697653	24.863332149524254
54-55	24.960100477611622	24.863773953757356	25.247276513890476	24.92884905474055
56-57	24.948570894703824	24.882580803861014	25.032477649995887	25.13637065143928
58-59	25.06794305792249	24.81269831508312	25.064040323524857	25.05531830346953
60-61	25.094710491128776	24.879969326069265	25.272646486791377	24.752673696010586
62-63	24.88581950375536	25.067970212549568	25.103359662058843	24.94285062163623
64-65	24.94510527045577	24.765586865828027	25.21003682906401	25.079271034652194
66-67	24.935234788120272	24.91010621110429	25.20162726752149	24.953031733253948
68-69	24.904760145147105	24.93044911352366	25.16623186584978	24.99855887547945
70-71	24.84124625789999	24.700417636840744	25.265003511106183	25.193332594153084
72-73	25.03118026197037	25.012467670524774	25.059736918110094	24.89661514939477
74-75	24.854507510324535	25.119224757478026	25.09128665176437	24.934981080433065
76-77	24.90896352332893	24.833048059100545	25.09065216941446	25.167336248156065
78-79	24.83037198731426	24.856357177825025	25.204481868899837	25.108788965960876
80-81	24.862473728861158	25.028658080573695	24.998714155229546	25.110154035335608
82-83	24.90397515516935	25.02462876551139	25.04452094575642	25.026875133562836
84-85	24.793543793189983	25.052354852544795	24.952152763552924	25.201948590712302
86-87	24.663817893711034	25.194644968936313	24.9982930417287	25.143244095623952
88-89	24.676739682036704	25.00824703993341	25.05968019220628	25.255333085823604
90-91	24.780381950088877	24.90856760145346	25.058388460749537	25.25266198770813
92-93	24.763420693650673	25.108073476662778	24.987119858473626	25.14138597121292
94-95	24.732597402290317	24.887239868435675	25.0607397301087	25.319422999165308
96-97	24.640084649454018	24.964081616970088	25.081154412840473	25.314679320735422
98-99	24.669252236068278	24.95260676113985	24.99306908979563	25.38507191299624
100-101	24.57919672756184	25.016329679228942	25.0821508478133	25.32232274539592
102-103	24.634453709488746	24.788728814511078	25.25046060871873	25.32635686728144
104-105	24.535402231724344	25.028815701276642	25.04725775009369	25.388524316905333
106-107	24.569593948971637	24.959664441253132	25.10300751501925	25.367734094755978
108-109	24.594759710973964	24.887870441333078	25.12958260760078	25.38778724009218
110-111	24.564409780202567	25.085575294733076	25.146605508383157	25.2034094166812
112-113	24.49908290069378	25.067873624129376	25.116196452542844	25.316847022633997
114-115	24.316581675930234	25.22741203946439	25.20016219861883	25.255844085986546
116-117	24.340047506471773	25.236357553437394	25.0812241265728	25.342370813518034
118-119	24.529579010168238	24.933689909905958	25.10893694720609	25.42779413271971
120-121	24.207792192385636	24.967650915190678	25.337007323364208	25.487549569059475
122-123	24.21669737050587	25.100211182503777	25.203082838879336	25.480008608111014
124-125	24.200782881233458	25.14115653043824	25.17777373875292	25.480286849575386
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	179.0
1	192.5
2	210.5
3	203.0
4	189.0
5	184.5
6	176.5
7	199.5
8	281.5
9	364.5
10	394.5
11	401.0
12	438.0
13	503.0
14	574.5
15	648.5
16	709.0
17	792.5
18	912.0
19	972.5
20	1064.0
21	1248.0
22	1400.5
23	1642.5
24	2037.5
25	2540.5
26	3244.0
27	4052.0
28	4976.0
29	6217.5
30	7827.0
31	9445.5
32	11174.5
33	13090.5
34	15142.5
35	17509.0
36	20376.5
37	23527.5
38	26479.0
39	29694.5
40	32983.0
41	35871.5
42	38989.0
43	43870.0
44	48779.0
45	52404.0
46	56979.5
47	61787.5
48	66513.0
49	71181.0
50	72652.0
51	72872.0
52	73349.0
53	73737.0
54	74804.0
55	74398.5
56	70855.0
57	65532.0
58	61556.5
59	56896.5
60	50532.5
61	45153.5
62	39242.0
63	30821.5
64	23496.5
65	18639.0
66	14277.0
67	10969.5
68	8852.0
69	7147.5
70	5944.0
71	4790.0
72	3779.0
73	3272.5
74	2594.0
75	1912.5
76	1253.0
77	738.5
78	474.0
79	296.5
80	170.5
81	79.5
82	53.5
83	38.0
84	25.5
85	21.5
86	16.5
87	16.0
88	16.0
89	12.0
90	11.0
91	10.5
92	8.0
93	7.5
94	7.0
95	6.0
96	7.0
97	10.5
98	9.5
99	6.5
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03351269818002998
2	0.02636095835677843
3	0.027011116522528574
4	0.03108938138041582
5	0.033926435194598245
6	0.04669317735841919
7	0.0380047000524855
8	0.03487211979932573
9	0.029670854473324598
10-11	0.03561093589676907
12-13	0.03552227796507587
14-15	0.03377867197510958
16-17	0.03762051568181496
18-19	0.04843678334838548
20-21	0.060523814702558555
22-23	0.05363804867438661
24-25	0.06466118484824127
26-27	0.04769796725094214
28-29	0.04908694151413562
30-31	0.04837767806059001
32-33	0.05691839214703504
34-35	0.03977785868634951
36-37	0.05112607394307925
38-39	0.06078978849763815
40-41	0.048554993923976414
42-43	0.05765720824447839
44-45	0.05839602434192173
46-47	0.06306534207776365
48-49	0.04811170426551041
50-51	0.05053502106512457
52-53	0.06067157792204722
54-55	0.04568838746589625
56-57	0.04332417595407755
58-59	0.045747492753691714
60-61	0.03295119794597304
62-63	0.04220117548596368
64-65	0.04163967525190674
66-67	0.03513809359440533
68-69	0.03005503884399514
70-71	0.04926425737752202
72-73	0.030823407585336218
74-75	0.038861726725519775
76-77	0.03197596069734783
78-79	0.0323896977119161
80-81	0.023701220405982402
82-83	0.016342612075446718
84-85	0.018588613011674478
86-87	0.01717008610458326
88-89	0.022460009362277586
90-91	0.012353005149252671
92-93	0.020007139918765692
94-95	0.014894532524457767
96-97	0.012618978944332275
98-99	0.011761952271297999
100-101	0.011289109968934261
102-103	0.006678897520887808
104-105	0.006501581657501407
106-107	0.008895345813217834
108-109	0.012678084232127743
110-111	0.006560686945296873
112-113	0.008806687881524633
114-115	0.008452056154751829
116-117	0.007476818906126618
118-119	0.2360074141673011
120-121	0.35407022653874703
122-123	0.027661274688278715
124-125	0.004048712213989512
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1691896.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.315931074318684
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.17651043190736	48.35920876990317
2	10.405565084212174	12.552426928173574
3	3.5890110107432656	6.49423622246885
4	1.7050415552358946	4.1136467569782935
5	1.0213130032969562	3.080072235608231
6	0.6592969139064461	2.3859664330015335
7	0.46452975662060825	1.961298134760905
8	0.35557621628995834	1.7157528442729726
9	0.2518760926134504	1.3672926937207424
>10	1.2969738545370995	13.438959603355622
>50	0.05539917974202056	2.255941681674323
>100	0.0182177034698128	1.9034468414699683
>500	3.9382709998692095E-4	0.14050519520952878
>1k	2.953703249901907E-4	0.23124565940232678
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1821057559093466E-4
2	0.0	0.0	0.0	0.0	1.1821057559093466E-4
3	0.0	0.0	0.0	0.0	1.1821057559093466E-4
4	0.0	0.0	0.0	0.0	1.77315863386402E-4
5	0.0	0.0	0.0	0.0	1.77315863386402E-4
6	0.0	0.0	0.0	0.0	1.77315863386402E-4
7	0.0	0.0	0.0	0.0	1.77315863386402E-4
8	0.0	0.0	0.0	0.0	1.77315863386402E-4
9	0.0	0.0	0.0	0.0	1.77315863386402E-4
10-11	0.0	0.0	0.0	0.0	1.77315863386402E-4
12-13	0.0	0.0	0.0	0.0	1.77315863386402E-4
14-15	0.0	0.0	0.0	0.0	2.6597379507960304E-4
16-17	0.0	0.0	0.0	5.910528779546733E-5	3.54631726772804E-4
18-19	0.0	0.0	0.0	1.1821057559093466E-4	3.54631726772804E-4
20-21	0.0	5.910528779546733E-5	0.0	1.1821057559093466E-4	5.319475901592061E-4
22-23	0.0	5.910528779546733E-5	0.0	1.77315863386402E-4	6.501581657501407E-4
24-25	0.0	5.910528779546733E-5	0.0	2.955264389773367E-4	6.501581657501407E-4
26-27	0.0	5.910528779546733E-5	0.0	3.54631726772804E-4	7.683687413410753E-4
28-29	0.0	8.8657931693201E-5	0.0	0.0011821057559093468	7.683687413410753E-4
30-31	0.0	1.1821057559093466E-4	0.0	0.004344238652966849	7.683687413410753E-4
32-33	0.0	1.4776321948866832E-4	0.0	0.009722819842354376	8.274740291365427E-4
34-35	0.0	1.77315863386402E-4	0.0	0.01749516518745833	8.274740291365427E-4
36-37	0.0	1.77315863386402E-4	0.0	0.028872933088085792	8.570266730342764E-4
38-39	0.0	1.77315863386402E-4	0.0	0.04542241367081665	8.8657931693201E-4
40-41	0.0	1.77315863386402E-4	0.0	0.0649567112872186	9.456846047274773E-4
42-43	0.0	1.77315863386402E-4	0.0	0.08357487694279081	9.75237248625211E-4
44-45	0.0	1.77315863386402E-4	0.0	0.10763072907554602	0.0010343425364206784
46-47	0.0	1.77315863386402E-4	0.0	0.13283913432031283	0.0010934478242161456
48-49	0.0	1.77315863386402E-4	0.0	0.15887501359421619	0.0011230004681138793
50-51	0.0	1.77315863386402E-4	0.0	0.18922557887718866	0.0011821057559093468
52-53	0.0	1.77315863386402E-4	0.0	0.2197239073796498	0.001241211043704814
54-55	0.0	2.955264389773367E-4	0.0	0.25320705291578205	0.0013003163315002814
56-57	0.0	2.955264389773367E-4	0.0	0.2904729368708242	0.0013003163315002814
58-59	0.0	2.955264389773367E-4	0.0	0.3319057436154468	0.0013003163315002814
60-61	0.0	2.955264389773367E-4	0.0	0.3776236837252408	0.0013889742631934825
62-63	0.0	2.955264389773367E-4	0.0	0.42629688822480816	0.0015367374826821506
64-65	0.0	2.955264389773367E-4	0.0	0.47529517180725056	0.0016549480582730854
66-67	0.0	2.955264389773367E-4	0.0	0.5304108526765239	0.0016549480582730854
68-69	0.0	2.955264389773367E-4	0.0	0.5949538269491742	0.0016549480582730854
70-71	0.0	2.955264389773367E-4	0.0	0.6667371989767692	0.0017140533460685526
72-73	0.0	2.955264389773367E-4	0.0	0.739998203199251	0.001950474497250422
74-75	0.0	3.2507908287507034E-4	0.0	0.8150028134116991	0.0021277903606368243
76-77	0.0	3.54631726772804E-4	0.0	0.8999666646176834	0.0021868956484322913
78-79	0.0	3.54631726772804E-4	0.0	0.9977859159191819	0.0021868956484322913
80-81	0.0	3.54631726772804E-4	0.0	1.10538709235083	0.002216448292330025
82-83	0.0	3.54631726772804E-4	0.0	1.2247502210537764	0.0022460009362277587
84-85	0.0	3.54631726772804E-4	0.0	1.3483098251902008	0.0022460009362277587
86-87	0.0	3.54631726772804E-4	0.0	1.478134589832945	0.00233465886792096
88-89	0.0	3.54631726772804E-4	0.0	1.6246270456340106	0.0023642115118186935
90-91	0.0	3.54631726772804E-4	0.0	1.7875507714422163	0.0023642115118186935
92-93	0.0	3.54631726772804E-4	0.0	1.959783580078208	0.002482422087409628
94-95	0.0	3.54631726772804E-4	0.0	2.1447831308780207	0.002571080019102829
96-97	0.0	3.54631726772804E-4	0.0	2.3340087097552096	0.0027188432385914976
98-99	0.0	3.54631726772804E-4	0.0	2.548206272725983	0.002748395882489231
100-101	5.910528779546733E-5	3.54631726772804E-4	0.0	2.7928135062675246	0.0027779485263869645
102-103	5.910528779546733E-5	3.54631726772804E-4	0.0	3.0606491179126847	0.002925711745875633
104-105	5.910528779546733E-5	3.54631726772804E-4	0.0	3.3351340744348352	0.003014369677568834
106-107	5.910528779546733E-5	3.54631726772804E-4	0.0	3.6158546388194073	0.003014369677568834
108-109	5.910528779546733E-5	3.54631726772804E-4	0.0	3.9194193969369273	0.003014369677568834
110-111	5.910528779546733E-5	3.54631726772804E-4	0.0	4.249670192494101	0.003073474965364301
112-113	5.910528779546733E-5	3.54631726772804E-4	0.0	4.609710053100191	0.0031325802531597686
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2115	0.0	70.07903	1
GTATCAA	3900	0.0	54.3356	1
TCAACGC	4950	0.0	41.46746	4
CAACGCA	5045	0.0	40.449543	5
AACGCAG	5330	0.0	38.394882	6
ATCAACG	5410	0.0	38.052677	3
TATCAAC	5475	0.0	37.92581	2
GTACATG	9140	0.0	36.730938	1
TACATGG	9385	0.0	35.75513	2
ACATGGG	9505	0.0	34.80399	3
ACGCAGA	6225	0.0	32.87466	7
CATGGGG	7990	0.0	32.764187	4
CGCAGAG	6800	0.0	30.093925	8
ATGGGGG	4270	0.0	28.563189	5
GCAGAGT	7435	0.0	27.283667	9
CAGAGTA	7090	0.0	24.876823	10-11
AGAGTAC	6750	0.0	24.058876	10-11
ATGGGGA	3135	0.0	23.152771	5
TACCGTA	180	2.180738E-5	23.134905	7
GTATAGG	495	0.0	22.847963	1
>>END_MODULE