FastQCFastQC Report
Mon 15 Aug 2016
SRR2921513_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921513_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1691896
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27760.16407627892021734No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24970.14758590362528193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24900.076.387071
GTATCAA48700.059.747451
TCAACGC64850.044.3480224
CAACGCA66850.042.817755
TATCAAC68600.041.7501532
AACGCAG69450.041.3763666
ATCAACG70250.041.108583
GTACATG86500.035.9818081
ACGCAGA81350.035.1764647
TACATGG89850.033.93042
ACATGGG90250.033.6480643
CGCAGAG86500.033.0104378
CATGGGG77400.030.69514
GCAGAGT94000.029.8070649
GTGGTAT10200.026.8897151
AGAGTAC86150.026.48111210-11
ATGGGGG42200.026.3699535
GAGTACT47000.026.32796512-13
CAGAGTA90000.026.14147610-11
AGTACTT49950.024.53485512-13
Produced by FastQC (version 0.11.5)