##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921513_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1691896
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1786752850057	31.0	31.0	34.0	28.0	34.0
2	31.2284307073248	33.0	31.0	34.0	28.0	34.0
3	31.315922491689797	33.0	31.0	34.0	28.0	34.0
4	34.83413637717685	37.0	35.0	37.0	32.0	37.0
5	34.47785088445153	37.0	35.0	37.0	30.0	37.0
6	34.456994992600016	37.0	35.0	37.0	30.0	37.0
7	34.296277667185215	37.0	35.0	37.0	30.0	37.0
8	34.25847628932275	37.0	35.0	37.0	30.0	37.0
9	35.82785821350721	39.0	37.0	39.0	30.0	39.0
10-11	35.693352901123944	39.0	35.0	39.0	30.0	39.0
12-13	35.59549286717387	39.0	35.0	39.0	30.0	39.0
14-15	36.75467315957955	40.0	37.0	41.0	30.0	41.0
16-17	36.72990124688515	40.0	37.0	41.0	30.0	41.0
18-19	36.743910086671995	40.0	37.0	41.0	30.0	41.0
20-21	36.72870200059578	40.0	37.0	41.0	30.0	41.0
22-23	36.66373967430622	40.0	37.0	41.0	30.0	41.0
24-25	36.59799065663611	40.0	36.0	41.0	29.5	41.0
26-27	36.46854357478237	40.0	36.0	41.0	29.0	41.0
28-29	36.284216937684114	40.0	36.0	41.0	28.5	41.0
30-31	36.233346198584314	40.0	36.0	41.0	29.0	41.0
32-33	36.11255863244549	40.0	35.0	41.0	28.5	41.0
34-35	35.987751906736584	40.0	35.0	41.0	28.0	41.0
36-37	35.85414233498986	40.0	35.0	41.0	27.0	41.0
38-39	35.687910781750176	40.0	35.0	41.0	27.0	41.0
40-41	35.478550100006146	39.0	35.0	41.0	26.0	41.0
42-43	35.3028829786228	39.0	35.0	41.0	25.5	41.0
44-45	35.09417895662617	39.0	35.0	41.0	24.5	41.0
46-47	34.820007553655785	39.0	34.5	41.0	23.5	41.0
48-49	34.557775714346505	39.0	34.0	41.0	23.0	41.0
50-51	34.33658836004104	38.5	34.0	41.0	22.0	41.0
52-53	34.12607394307925	38.0	34.0	40.5	22.0	41.0
54-55	33.90341279842259	38.0	33.0	40.0	21.0	41.0
56-57	33.636440123979256	37.5	33.0	40.0	20.0	41.0
58-59	33.478386673885396	37.0	33.0	40.0	20.0	41.0
60-61	33.3548060873718	37.0	33.0	40.0	20.0	41.0
62-63	33.0790583463759	36.0	33.0	40.0	19.5	41.0
64-65	32.77989013509105	36.0	33.0	39.0	18.0	41.0
66-67	32.47843129837767	35.0	32.5	39.0	18.0	41.0
68-69	32.15639347808612	35.0	32.0	38.5	17.0	40.5
70-71	31.853091442972854	35.0	32.0	37.0	15.0	40.0
72-73	31.539296741643696	35.0	32.0	37.0	13.0	39.0
74-75	31.261710826197355	35.0	32.0	36.0	12.5	39.0
76-77	30.978662695579395	35.0	31.5	36.0	10.5	38.5
78-79	30.71096745899275	35.0	31.0	35.5	8.0	37.0
80-81	30.49643122272291	35.0	31.0	35.0	7.0	37.0
82-83	30.318948091372047	35.0	31.0	35.0	7.0	36.5
84-85	30.13803064727383	35.0	31.0	35.0	7.0	36.0
86-87	30.000278385905517	35.0	31.0	35.0	3.5	36.0
88-89	29.904009761829332	35.0	31.0	35.0	2.0	35.5
90-91	29.807155404351093	35.0	31.0	35.0	2.0	35.0
92-93	29.87625066788975	35.0	31.0	35.0	2.0	35.0
94-95	29.841390073621547	35.0	31.0	35.0	2.0	35.0
96-97	29.826842784662887	35.0	31.0	35.0	2.0	35.0
98-99	29.786446980192636	35.0	31.0	35.0	2.0	35.0
100-101	29.737343193671478	35.0	31.0	35.0	2.0	35.0
102-103	28.84864022374898	33.5	29.5	35.0	2.0	35.0
104-105	29.557733454065733	34.5	31.0	35.0	2.0	35.0
106-107	29.666545993370754	35.0	31.0	35.0	2.0	35.0
108-109	29.673620896319868	35.0	31.0	35.0	2.0	35.0
110-111	29.64330668078889	35.0	31.0	35.0	2.0	35.0
112-113	29.594905065086742	35.0	31.0	35.0	2.0	35.0
114-115	29.52621585487524	35.0	31.0	35.0	2.0	35.0
116-117	29.45030131875718	35.0	31.0	35.0	2.0	35.0
118-119	29.35536670102654	35.0	31.0	35.0	2.0	35.0
120-121	29.234575293044017	35.0	31.0	35.0	2.0	35.0
122-123	29.11106267761139	35.0	31.0	35.0	2.0	35.0
124-125	28.18730022412725	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	29577.0
3	10897.0
4	6302.0
5	5086.0
6	5592.0
7	6558.0
8	7443.0
9	8119.0
10	9243.0
11	9738.0
12	10404.0
13	11892.0
14	14461.0
15	11819.0
16	7017.0
17	6067.0
18	5562.0
19	5021.0
20	4896.0
21	5119.0
22	6219.0
23	8134.0
24	11637.0
25	16070.0
26	19680.0
27	22276.0
28	26068.0
29	31138.0
30	35768.0
31	43098.0
32	54138.0
33	69723.0
34	96957.0
35	154531.0
36	305999.0
37	472375.0
38	137012.0
39	260.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.44828884017971	17.14858377794926	12.330517300964347	25.07261008090669
2	17.333945673163026	19.302771901640256	37.18344956559927	26.17983285959745
3	19.628935751602263	23.740343704127607	28.61698372995064	28.01373681431949
4	13.828363535722755	15.283850025517731	34.413514995141874	36.47427144361764
5	14.68846054476024	36.85494403233254	32.87533632923284	15.58125909367438
6	34.50721710918361	34.08269170741858	16.39988743958154	15.010203743816271
7	30.21060988607901	31.184710905757886	19.809873546577787	18.79480566158532
8	28.435504349203573	33.228612770746494	18.61687287652707	19.719010003522865
9	27.940088189050844	14.340118925181166	18.27132910120462	39.44846378456337
10-11	26.92230167263579	24.52205128265754	26.154167166638786	22.401479878067885
12-13	27.197251713836827	22.07772940115308	26.699030273941997	24.025988611068094
14-15	24.542825604780617	23.114699593488126	24.218827809490598	28.123646992240666
16-17	24.627818515530098	26.189931519197973	24.683146983977796	24.49910298129413
18-19	24.67135478669389	24.991310998856836	26.648752744128423	23.688581470320855
20-21	25.580642682335693	23.963089509712354	26.604725535864183	23.851542272087773
22-23	25.356873586699614	23.582458420780256	26.543166638145742	24.517501354374385
24-25	24.803223150120267	24.104196112995872	26.5285637521525	24.564016984731357
26-27	24.54989596387109	24.18186815785118	26.58885631191923	24.6793795663585
28-29	24.652165392906134	24.343678592044604	26.565362876183503	24.438793138865762
30-31	24.93502808158439	24.210168489506355	26.464085131540056	24.390718297369197
32-33	24.595289904548416	24.248467318459035	26.384704101279056	24.771538675713494
34-35	24.793460007845106	24.320122873153156	26.47424579537517	24.412171323626566
36-37	24.940360124059218	24.183300952967038	26.30476915059989	24.57156977237386
38-39	25.030261199599767	24.303046515786814	26.224688113814626	24.442004170798793
40-41	25.005482608456635	24.23785831284621	26.232079554865393	24.52457952383176
42-43	24.93024113616877	24.29668494293236	26.09512032277199	24.677953598126876
44-45	24.86299790728195	24.362725972168032	25.998149658898782	24.776126461651238
46-47	24.968291994449064	24.470777056734498	25.734220260905676	24.82671068791076
48-49	25.180371384147893	24.692168535805287	25.26630052438354	24.861159555663285
50-51	25.08580552560392	24.94378156744793	25.144299888390748	24.826113018557404
52-53	25.258009268647108	24.746868033217677	25.24160379873829	24.75351889939693
54-55	24.974978821412336	24.796682602580923	25.417615737360684	24.81072283864606
56-57	24.97355911201182	24.798951705252527	25.178671824979943	25.04881735775571
58-59	25.00764813664789	24.68221438015215	25.33834321999706	24.971794263202902
60-61	25.056450089666892	24.783798890773102	25.486886641953465	24.67286437760654
62-63	24.9402745155304	24.893510983518073	25.324551327262547	24.84166317368898
64-65	24.914303212776026	24.60976445317661	25.48152623487762	24.994406099169737
66-67	24.943672774509327	24.76160497494351	25.430556356043038	24.864165894504122
68-69	24.993518945224224	24.81178337048547	25.256012748685535	24.938684935604773
70-71	24.868506030470837	24.629119762594083	25.45554772281559	25.04682648411949
72-73	25.09043336484239	24.913202295845828	25.16964938634186	24.826714952969922
74-75	24.93235530064278	25.036401331552728	25.122032397332873	24.90921097047162
76-77	24.955538931403304	24.77754687217823	25.12974769876839	25.13716649765007
78-79	24.87859690032249	24.812940046512605	25.220361369883992	25.088101683280918
80-81	24.96593278545961	25.034244570971836	24.98015085938059	25.019671784187963
82-83	24.998234062003192	24.954285111354583	25.02838061860143	25.019100208040797
84-85	24.825401254208504	24.961568916295164	24.99949014713024	25.213539682366093
86-87	24.69908504830127	25.0784725662273	25.07058097482139	25.151861410650046
88-89	24.728409308572903	24.860214151092865	25.107802704792626	25.30357383554161
90-91	24.741593723352846	24.79538680030207	25.138066434450035	25.324953041895053
92-93	24.758062323334663	24.986256899627488	25.064223133568746	25.1914576434691
94-95	24.74651421018861	24.812421845514276	25.096740882210643	25.34432306208647
96-97	24.675871990788764	24.81329576492437	25.15650055826561	25.354331686021254
98-99	24.686617466436388	24.796289531888537	24.972988340656627	25.54410466101845
100-101	24.56970315325407	24.90827171316578	25.122171378646446	25.399853754933705
102-103	24.63861660022981	24.666810889024443	25.27700740971917	25.41756510102658
104-105	24.571622779797263	24.938055974229396	25.019357508053314	25.470963737920027
106-107	24.625899474050197	24.76299455372877	25.10149143724642	25.509614534974613
108-109	24.637475532665185	24.71589515122601	25.06374365149884	25.58288566460996
110-111	24.610773452742833	24.894924643045226	25.083708605983517	25.410593298228424
112-113	24.562867798856384	24.91499119395958	25.04385286952194	25.4782881376621
114-115	24.329669498277628	25.01462893917435	25.150042675127608	25.505658887420413
116-117	24.330805681320783	25.0100709056781	25.05830485451642	25.600818558484693
118-119	24.334593680609103	24.722480751207566	25.178138703616625	25.76478686456671
120-121	24.13810428159447	24.76993751710433	25.294342256843656	25.797615944457547
122-123	24.196758693512155	24.812730457458702	25.158351998472707	25.832158850556436
124-125	24.130372964603627	24.91319839598085	25.088693414901915	25.86773522451361
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	64.0
1	59.0
2	85.0
3	128.5
4	97.5
5	88.0
6	183.5
7	360.0
8	559.5
9	648.5
10	535.5
11	401.5
12	456.0
13	605.0
14	681.0
15	781.5
16	916.0
17	1050.0
18	1217.0
19	1342.0
20	1518.5
21	1739.0
22	1959.0
23	2244.0
24	2547.0
25	3056.0
26	3738.5
27	4493.5
28	5352.0
29	6505.5
30	8014.0
31	9536.0
32	11159.5
33	12930.5
34	14890.5
35	17025.0
36	19816.5
37	22844.0
38	25671.5
39	28993.0
40	32350.5
41	35347.0
42	38502.0
43	43281.5
44	48091.5
45	51861.0
46	56603.0
47	61376.0
48	66574.5
49	71376.0
50	72747.5
51	73430.5
52	73987.5
53	74508.0
54	75357.0
55	74768.0
56	71606.0
57	66190.5
58	61917.0
59	57109.0
60	50826.0
61	44997.0
62	38902.5
63	30669.5
64	23011.0
65	18239.0
66	13934.5
67	10560.0
68	8457.0
69	6668.0
70	5627.0
71	4626.0
72	3608.5
73	3271.5
74	2592.5
75	1861.0
76	1288.0
77	682.5
78	384.0
79	232.5
80	118.5
81	43.5
82	17.5
83	7.5
84	5.5
85	5.0
86	4.0
87	3.5
88	3.5
89	2.0
90	0.0
91	0.0
92	0.5
93	0.5
94	0.5
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12104762940511711
2	0.0014776321948866834
3	0.022637325225663987
4	0.054081338332852606
5	0.027956801127256046
6	0.02127790360636824
7	0.01684500702170819
8	0.005437686477182994
9	0.004492001872455517
10-11	0.00797921385238809
12-13	0.004610212448046451
14-15	0.0037531857750121756
16-17	0.010698057090979587
18-19	0.006028739355137668
20-21	0.01394884791973029
22-23	0.00919087225219517
24-25	0.01530826953902604
26-27	0.010402530652002251
28-29	0.014864979880560035
30-31	0.023405693967005065
32-33	0.014953637812253236
34-35	0.02337614132310733
36-37	0.016815454377810454
38-39	0.02207582499160705
40-41	0.010816267666570522
42-43	0.010372978008104517
44-45	0.01867727094336768
46-47	0.017051875528992327
48-49	0.022814641089050392
50-51	0.01654948058273085
52-53	0.022873746376845857
54-55	0.019445639684708752
56-57	0.022873746376845857
58-59	0.018174875997106203
60-61	0.021130140386879572
62-63	0.024055852132755205
64-65	0.01900235002624275
66-67	0.012914505383309613
68-69	0.025267510532562286
70-71	0.01646082265103765
72-73	0.01885458680675408
74-75	0.01991848198707249
76-77	0.014628558729378165
78-79	0.03111893402431355
80-81	0.022873746376845857
82-83	0.009486398691172508
84-85	0.013682874124650688
86-87	0.013121373890593747
88-89	0.022460009362277586
90-91	0.013505558261264287
92-93	0.008156529715774493
94-95	0.007949661208490357
96-97	0.0028961591019778994
98-99	0.0020095797850458895
100-101	0.012707636876025476
102-103	0.0037827384189099097
104-105	0.002718843238591497
106-107	2.65973795079603E-4
108-109	0.020982377167390903
110-111	8.8657931693201E-4
112-113	0.00942729340337704
114-115	0.0026006326630005627
116-117	0.008363398223058626
118-119	0.009841030417945311
120-121	0.004314686009069116
122-123	9.752372486252109E-4
124-125	0.023346588679209596
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1691896.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.05238819771608
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.64666458511842	47.033257557336974
2	10.681595441587016	12.615474411751018
3	3.600894342255914	6.379242316735673
4	1.7999181573136136	4.251578629992052
5	1.0401634183885435	3.0712066985871815
6	0.6997787829673926	2.4794165006589464
7	0.467929972049431	1.9342667651166605
8	0.34688569267848784	1.638754286742696
9	0.27594242379073086	1.4665553216918028
>10	1.3586451889674285	13.942260661212845
>50	0.059029104781137945	2.341403584324702
>100	0.021751137144627748	2.2527532492967355
>500	4.008764786474547E-4	0.1329222859455148
>1k	4.008764786474547E-4	0.4609077306071546
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2776	0.16407627892021734	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2497	0.14758590362528193	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.910528779546733E-5
2	0.0	0.0	0.0	0.0	5.910528779546733E-5
3	0.0	0.0	0.0	0.0	5.910528779546733E-5
4	0.0	0.0	0.0	0.0	5.910528779546733E-5
5	0.0	0.0	0.0	0.0	5.910528779546733E-5
6	0.0	0.0	0.0	0.0	5.910528779546733E-5
7	0.0	0.0	0.0	0.0	5.910528779546733E-5
8	0.0	0.0	0.0	0.0	5.910528779546733E-5
9	0.0	0.0	0.0	0.0	1.1821057559093466E-4
10-11	0.0	0.0	0.0	0.0	1.77315863386402E-4
12-13	0.0	0.0	0.0	0.0	2.3642115118186933E-4
14-15	0.0	0.0	0.0	0.0	3.2507908287507034E-4
16-17	0.0	0.0	0.0	0.0	3.54631726772804E-4
18-19	0.0	0.0	0.0	5.910528779546733E-5	3.8418437067053765E-4
20-21	0.0	0.0	0.0	5.910528779546733E-5	5.615002340569397E-4
22-23	0.0	0.0	0.0	1.1821057559093466E-4	6.501581657501407E-4
24-25	0.0	0.0	0.0	2.3642115118186933E-4	7.388160974433416E-4
26-27	0.0	0.0	0.0	2.955264389773367E-4	7.683687413410753E-4
28-29	0.0	0.0	0.0	0.001152553112011613	8.570266730342764E-4
30-31	0.0	0.0	0.0	0.004403343940762317	8.8657931693201E-4
32-33	0.0	0.0	0.0	0.009959240993536246	9.456846047274773E-4
34-35	0.0	0.0	0.0	0.017761138982537936	0.0010047898925229447
36-37	0.0	0.0	0.0	0.0295821965416314	0.0010047898925229447
38-39	0.0	0.0	0.0	0.046308992987748654	0.0010047898925229447
40-41	0.0	0.0	0.0	0.06631613290651435	0.001063895180318412
42-43	0.0	0.0	0.0	0.08561400937173444	0.0011230004681138793
44-45	0.0	0.0	0.0	0.10990628265567151	0.0011821057559093465
46-47	0.0	0.0	0.0	0.13541021433941566	0.0013003163315002814
48-49	0.0	0.0	0.0	0.16156430418890996	0.0013889742631934825
50-51	0.0	0.0	0.0	0.19226950119865524	0.001418526907091216
52-53	0.0	0.0	0.0	0.22324067200348013	0.0015367374826821506
54-55	0.0	0.0	0.0	0.25769905478823757	0.001595842770477618
56-57	0.0	0.0	0.0	0.2956446495529276	0.0017140533460685526
58-59	0.0	0.0	0.0	0.337875377682789	0.0017140533460685526
60-61	0.0	0.0	0.0	0.3845094497534127	0.0017140533460685526
62-63	0.0	0.0	0.0	0.4333895227602642	0.001950474497250422
64-65	0.0	0.0	0.0	0.48318572772794544	0.0020095797850458895
66-67	0.0	0.0	0.0	0.5391879879141508	0.002039132428943623
68-69	0.0	0.0	0.0	0.6044106729964489	0.002068685072841357
70-71	0.0	2.9552643897733666E-5	0.0	0.6769624137653851	0.002127790360636824
72-73	0.0	5.910528779546733E-5	0.0	0.7517897081144467	0.002393764155716427
74-75	0.0	5.910528779546733E-5	0.0	0.8280059767267018	0.002482422087409628
76-77	0.0	5.910528779546733E-5	0.0	0.9153340394445049	0.002482422087409628
78-79	0.0	5.910528779546733E-5	0.0	1.0134192645410829	0.002482422087409628
80-81	0.0	5.910528779546733E-5	0.0	1.1226458363871066	0.0025119747313073616
82-83	0.0	5.910528779546733E-5	0.0	1.2429546496947803	0.0025415273752050953
84-85	0.0	5.910528779546733E-5	0.0	1.3677554648749095	0.0025415273752050953
86-87	0.0	5.910528779546733E-5	0.0	1.4977279927371423	0.0026006326630005627
88-89	0.0	5.910528779546733E-5	0.0	1.6474712393669586	0.00265973795079603
90-91	0.0	5.910528779546733E-5	0.0	1.8135866507161196	0.00265973795079603
92-93	2.9552643897733666E-5	5.910528779546733E-5	0.0	1.9902523559367715	0.00265973795079603
94-95	5.910528779546733E-5	5.910528779546733E-5	0.0	2.17726148652163	0.0027779485263869645
96-97	5.910528779546733E-5	5.910528779546733E-5	0.0	2.3697674088714673	0.002925711745875633
98-99	5.910528779546733E-5	5.910528779546733E-5	0.0	2.5868906835881162	0.003014369677568834
100-101	5.910528779546733E-5	5.910528779546733E-5	0.0	2.8362854454410913	0.003014369677568834
102-103	5.910528779546733E-5	5.910528779546733E-5	0.0	3.1097360594268206	0.003103027609262035
104-105	5.910528779546733E-5	5.910528779546733E-5	0.0	3.389629149782256	0.0031325802531597686
106-107	5.910528779546733E-5	5.910528779546733E-5	0.0	3.6748121633953863	0.0031621328970575023
108-109	5.910528779546733E-5	5.910528779546733E-5	0.0	3.9842283450046576	0.0032212381848529697
110-111	5.910528779546733E-5	5.910528779546733E-5	0.0	4.323197170511662	0.0033394487604439045
112-113	5.910528779546733E-5	5.910528779546733E-5	0.0	4.692752982452822	0.0033690014043416382
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2490	0.0	76.38707	1
GTATCAA	4870	0.0	59.74745	1
TCAACGC	6485	0.0	44.348022	4
CAACGCA	6685	0.0	42.81775	5
TATCAAC	6860	0.0	41.750153	2
AACGCAG	6945	0.0	41.376366	6
ATCAACG	7025	0.0	41.10858	3
GTACATG	8650	0.0	35.981808	1
ACGCAGA	8135	0.0	35.176464	7
TACATGG	8985	0.0	33.9304	2
ACATGGG	9025	0.0	33.648064	3
CGCAGAG	8650	0.0	33.010437	8
CATGGGG	7740	0.0	30.6951	4
GCAGAGT	9400	0.0	29.807064	9
GTGGTAT	1020	0.0	26.889715	1
AGAGTAC	8615	0.0	26.481112	10-11
ATGGGGG	4220	0.0	26.369953	5
GAGTACT	4700	0.0	26.327965	12-13
CAGAGTA	9000	0.0	26.141476	10-11
AGTACTT	4995	0.0	24.534855	12-13
>>END_MODULE