FastQCFastQC Report
Mon 15 Aug 2016
SRR2921512_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921512_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1851270
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20150.066.463571
GTATCAA39450.062.162211
TCAACGC50650.047.57084
CAACGCA52750.045.56425
ATCAACG55250.043.5024763
AACGCAG55150.043.4687846
TATCAAC58000.041.4398572
ACGCAGA66250.036.0949447
GTACATG100150.035.303011
TACATGG102750.033.6981472
CGCAGAG71950.033.1500788
ACATGGG104850.032.8529973
CATGGGG86750.031.203754
GCAGAGT76700.030.4767179
AGAGTAC68300.025.86570710-11
ATGGGGG45000.025.119215
CAGAGTA73700.024.65655110-11
GTATAGG6650.024.1667541
ATGGGGA35250.024.1346935
GTACGTA1751.746537E-523.808731
Produced by FastQC (version 0.11.5)