FastQCFastQC Report
Mon 15 Aug 2016
SRR2921512_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921512_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1851270
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28630.15465059121576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25210.13617678674639572No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27300.083.435281
GTATCAA55850.058.1865161
TCAACGC72850.044.465084
CAACGCA74150.043.576945
TATCAAC76300.042.5348782
ATCAACG77100.042.0140273
AACGCAG77350.041.7651186
ACGCAGA88850.036.357427
GTGGTAT10750.034.3900031
CGCAGAG94700.033.797388
GTACATG92900.033.697111
TACATGG97650.031.7105922
ACATGGG98250.031.3940223
GCAGAGT101100.031.3636639
CATGGGG81450.029.242784
CAGAGTA97650.027.59831210-11
GAGTACT49400.027.21759812-13
AGAGTAC91650.027.00333810-11
GTATAGG6200.026.92871
TGGTATC12850.024.5609952
Produced by FastQC (version 0.11.5)