##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921512_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1851270
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.204438034430417	31.0	31.0	34.0	28.0	34.0
2	31.277377151901128	33.0	31.0	34.0	28.0	34.0
3	31.359632036385833	33.0	31.0	34.0	28.0	34.0
4	34.87532612746925	37.0	35.0	37.0	32.0	37.0
5	34.533691465858574	37.0	35.0	37.0	31.0	37.0
6	34.50995208694572	37.0	35.0	37.0	30.0	37.0
7	34.35739033204233	37.0	35.0	37.0	30.0	37.0
8	34.331840844393305	37.0	35.0	37.0	30.0	37.0
9	35.904816153235345	39.0	37.0	39.0	30.0	39.0
10-11	35.76918547807721	39.0	36.0	39.0	30.0	39.0
12-13	35.67834702663577	39.0	35.0	39.0	30.0	39.0
14-15	36.841430207371154	40.0	37.0	41.0	30.0	41.0
16-17	36.812387172049455	40.0	37.0	41.0	30.0	41.0
18-19	36.82309009490782	40.0	37.0	41.0	30.0	41.0
20-21	36.79870197215965	40.0	37.0	41.0	30.0	41.0
22-23	36.73219249488189	40.0	37.0	41.0	30.0	41.0
24-25	36.65577819550903	40.0	36.5	41.0	30.0	41.0
26-27	36.52124244437602	40.0	36.0	41.0	29.5	41.0
28-29	36.32758106597093	40.0	36.0	41.0	28.5	41.0
30-31	36.28606038017145	40.0	36.0	41.0	29.0	41.0
32-33	36.16176786746396	40.0	35.5	41.0	28.5	41.0
34-35	36.036184619207354	40.0	35.0	41.0	28.0	41.0
36-37	35.89811696835146	40.0	35.0	41.0	27.0	41.0
38-39	35.72375531391964	40.0	35.0	41.0	27.0	41.0
40-41	35.51876171493083	39.0	35.0	41.0	26.0	41.0
42-43	35.348125340982136	39.0	35.0	41.0	25.5	41.0
44-45	35.1283084045007	39.0	35.0	41.0	24.5	41.0
46-47	34.85192219395334	39.0	34.5	41.0	23.5	41.0
48-49	34.59289568782512	39.0	34.0	41.0	23.0	41.0
50-51	34.371671069049896	39.0	34.0	41.0	22.0	41.0
52-53	34.16875253204557	38.0	34.0	40.5	22.0	41.0
54-55	33.9387693313239	38.0	33.0	40.0	21.0	41.0
56-57	33.67504604946875	37.5	33.0	40.0	20.0	41.0
58-59	33.52706250303845	37.0	33.0	40.0	20.0	41.0
60-61	33.40401481145376	37.0	33.0	40.0	20.0	41.0
62-63	33.13386999195147	36.0	33.0	40.0	19.5	41.0
64-65	32.835908592479754	36.0	33.0	39.0	18.5	41.0
66-67	32.536692108660546	35.0	33.0	39.0	18.0	41.0
68-69	32.22053509212594	35.0	32.0	38.5	17.0	40.5
70-71	31.9198728440476	35.0	32.0	37.5	15.5	40.0
72-73	31.60812820388166	35.0	32.0	37.0	13.0	39.0
74-75	31.32083947776391	35.0	32.0	36.5	12.5	39.0
76-77	31.036519524434578	35.0	32.0	36.0	10.5	39.0
78-79	30.76965650607421	35.0	31.0	35.5	8.0	37.0
80-81	30.548079696640684	35.0	31.0	35.0	7.0	37.0
82-83	30.36830959287408	35.0	31.0	35.0	7.0	36.5
84-85	30.181958331307698	35.0	31.0	35.0	7.0	36.0
86-87	30.045457712813366	35.0	31.0	35.0	5.5	36.0
88-89	29.944771967352143	35.0	31.0	35.0	2.0	35.5
90-91	29.844806538214307	35.0	31.0	35.0	2.0	35.0
92-93	29.909721704559573	35.0	31.0	35.0	2.0	35.0
94-95	29.883932921724007	35.0	31.0	35.0	2.0	35.0
96-97	29.866095167101506	35.0	31.0	35.0	2.0	35.0
98-99	29.830449907360894	35.0	31.0	35.0	2.0	35.0
100-101	29.77983033269053	35.0	31.0	35.0	2.0	35.0
102-103	28.887544766565654	33.5	29.5	35.0	2.0	35.0
104-105	29.598683876473988	34.5	31.0	35.0	2.0	35.0
106-107	29.70991616565925	35.0	31.0	35.0	2.0	35.0
108-109	29.71300242536205	35.0	31.0	35.0	2.0	35.0
110-111	29.681340107061637	35.0	31.0	35.0	2.0	35.0
112-113	29.63523067947949	35.0	31.0	35.0	2.0	35.0
114-115	29.568307162110337	35.0	31.0	35.0	2.0	35.0
116-117	29.48388160560048	35.0	31.0	35.0	2.0	35.0
118-119	29.385948565039136	35.0	31.0	35.0	2.0	35.0
120-121	29.261653081398173	35.0	31.0	35.0	2.0	35.0
122-123	29.137337341392666	35.0	31.0	35.0	2.0	35.0
124-125	28.205804663825376	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	31410.0
3	11655.0
4	6722.0
5	5591.0
6	6002.0
7	7153.0
8	8212.0
9	8988.0
10	10138.0
11	10867.0
12	11408.0
13	12922.0
14	16144.0
15	13389.0
16	7714.0
17	6358.0
18	5846.0
19	5484.0
20	5255.0
21	5646.0
22	6598.0
23	8975.0
24	12613.0
25	17299.0
26	21052.0
27	23764.0
28	27972.0
29	33351.0
30	39126.0
31	46982.0
32	58805.0
33	75382.0
34	104577.0
35	168038.0
36	332579.0
37	518875.0
38	158150.0
39	228.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.6379280507063	17.650669522140742	12.707887165509336	25.003515261643628
2	17.029578140395152	19.890483742718363	37.07082634699487	26.009111769891618
3	19.222490326108215	24.21212216172175	29.061440693991653	27.50394681817838
4	13.680297437954039	15.65597467518394	35.05446165038285	35.60926623647917
5	14.47411646725223	37.02435550522166	33.18580771491804	15.315720312608065
6	33.72723520220608	34.702769055219015	16.630377700601443	14.939618041973457
7	29.643134023960343	31.523818528073043	20.282607814800308	18.550439633166306
8	28.127218215703753	32.9783648002593	19.165653782783675	19.728763201253273
9	28.01962195169383	14.231857095320205	18.635614242066733	39.11290671091924
10-11	26.520273874699274	24.67249209067825	26.546203863190136	22.261030171432335
12-13	26.935642794171034	22.42827231239952	26.920598238517517	23.715486654911928
14-15	24.35257245122247	23.320653345296673	24.52659367300648	27.80018053047438
16-17	24.19426767678814	26.371852520536155	25.000965660569907	24.4329141421058
18-19	24.314656961821203	25.187482629243156	27.030288657749974	23.467571751185666
20-21	25.4013376887498	24.158244997308252	26.818225651306825	23.622191662635124
22-23	25.174809308144912	23.9031521183347	26.69297010920872	24.229068464311673
24-25	24.59508015739477	24.4217968038608	26.679207537272127	24.3039155014723
26-27	24.280020690651796	24.430446591242127	26.840745457713144	24.448787260392933
28-29	24.417694701222352	24.48536143082853	26.8548044863717	24.242139381577417
30-31	24.660048086014534	24.37925817867466	26.68276737715104	24.277926358159764
32-33	24.288134215127673	24.518820517682755	26.650669895194408	24.54237537199517
34-35	24.475071838672914	24.505733451441326	26.762185019465395	24.257009690420368
36-37	24.71508166803442	24.379716786180644	26.49125944302687	24.413942102758067
38-39	24.714345301258458	24.59548009656177	26.3584942155869	24.33168038659287
40-41	24.667344299898893	24.467971025101694	26.47561479278928	24.38906988221013
42-43	24.666996745937013	24.53175068156507	26.296054094103926	24.50519847839399
44-45	24.6347836633983	24.58078359262282	26.14975713474922	24.634675609229664
46-47	24.788214573214546	24.725570388939612	25.873830993068346	24.612384044777492
48-49	24.94584876555717	24.89225133520816	25.461534928829423	24.70036497040525
50-51	24.804838761524703	25.10133203167313	25.331103510889836	24.762725695912334
52-53	25.03482916621529	25.002681244713493	25.364656034799722	24.5978335542715
54-55	24.844206569837787	24.92654362391632	25.46824472150059	24.761005084745307
56-57	24.72756979124625	25.06179623209125	25.292394106246835	24.918239870415665
58-59	24.797810390326774	24.80272685499968	25.52247567227927	24.876987082394276
60-61	24.849118293257145	24.91903134227921	25.63966522779417	24.592185136669475
62-63	24.72807168971199	24.98952485385449	25.547037661088297	24.735365795345228
64-65	24.74893105247973	24.720188061285246	25.686455027484133	24.844425858750892
66-67	24.71314930552648	24.975358001173955	25.55099077177741	24.760501921522156
68-69	24.829990047407193	24.892099197416865	25.422579958439012	24.855330796736933
70-71	24.65583316292585	24.73857421207587	25.706479707372758	24.89911291762552
72-73	24.9704737916406	24.958020377135856	25.327516697300446	24.7439891339231
74-75	24.674748254068078	25.170946266466544	25.2365909294744	24.917714549990976
76-77	24.721751079351627	24.858137148361518	25.35902977613938	25.06108199614748
78-79	24.65838472399258	24.922637087836826	25.36792974307462	25.051048445095976
80-81	24.694863583132637	25.056804904766857	25.152949789353823	25.09538172274668
82-83	24.760504548968466	25.062868737638965	25.18947057364921	24.987156139743362
84-85	24.61447657739912	25.01150735552326	25.15961728588485	25.214398781192767
86-87	24.464366317064453	25.067415951898653	25.283703451388796	25.184514279648095
88-89	24.566084618935996	24.922218191042443	25.263466714356454	25.248230475665107
90-91	24.492247433819557	24.879011345218803	25.324041058887087	25.304700162074557
92-93	24.517589197952454	25.044710059216353	25.253046375021032	25.18465436781016
94-95	24.46293724766836	24.831715880818567	25.316451935203222	25.388894936309853
96-97	24.47200664215293	24.82985425184758	25.32663824196862	25.37150086403087
98-99	24.367299380790403	24.791773690047325	25.280259459786485	25.56066746937578
100-101	24.334150900584543	24.852486737042277	25.37703536428564	25.436326998087537
102-103	24.474210826691	24.66457450288152	25.432889646598262	25.428325023829224
104-105	24.279380235401646	24.8010094969855	25.31802018021639	25.601590087396463
106-107	24.302563414190807	24.794471229483246	25.291591706520123	25.611373649805824
108-109	24.290070153514336	24.72124587930159	25.291167205467485	25.69751676171659
110-111	24.243342540623217	24.820709028842934	25.306353732247473	25.629594698286372
112-113	24.28083914210129	24.778278206086064	25.31834101262992	25.622541639182728
114-115	24.006900299477536	25.00877797608501	25.259801082959164	25.72452064147829
116-117	24.034107315196895	24.90070085411446	25.278854690876795	25.78633713981185
118-119	23.98453744976508	24.6696069750569	25.34730231927449	25.998553255903534
120-121	23.748611699323245	24.76631172265966	25.449090960163872	26.035985617853225
122-123	23.73692441073758	24.771303791211906	25.413840880915917	26.077930917134594
124-125	23.768262607111858	24.84606068530517	25.340627795228087	26.045048912354886
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	36.0
1	39.5
2	64.0
3	91.0
4	65.0
5	58.0
6	137.5
7	272.0
8	448.0
9	558.5
10	493.5
11	426.0
12	464.5
13	545.5
14	651.5
15	776.0
16	914.0
17	1052.0
18	1220.5
19	1394.5
20	1591.0
21	1868.5
22	2103.0
23	2395.0
24	2802.5
25	3402.5
26	4435.5
27	5639.5
28	6562.5
29	7881.5
30	9959.5
31	11978.0
32	14487.5
33	17034.0
34	19681.5
35	22627.5
36	26212.0
37	30099.5
38	32774.5
39	35449.5
40	38053.0
41	40365.5
42	43230.0
43	48762.5
44	54241.5
45	58553.0
46	63089.0
47	66606.5
48	71610.0
49	76129.5
50	76366.0
51	76111.5
52	76203.5
53	76577.0
54	78090.0
55	77609.5
56	73631.5
57	67827.0
58	64224.5
59	60551.0
60	54221.5
61	47881.5
62	41248.0
63	32752.5
64	25154.0
65	19914.0
66	15464.0
67	11947.0
68	9611.0
69	7761.5
70	6449.0
71	5280.0
72	4268.0
73	3781.0
74	3061.5
75	2341.0
76	1602.0
77	885.0
78	491.5
79	307.5
80	180.5
81	70.5
82	40.5
83	18.0
84	9.0
85	7.5
86	4.5
87	5.0
88	3.5
89	0.5
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11829716897049053
2	0.002052644941040475
3	0.02225499251864936
4	0.05774414320979652
5	0.02798079156470963
6	0.020850551243200614
7	0.01593500677913
8	0.006482036655917288
9	0.004213323826346238
10-11	0.007103231835442696
12-13	0.005509731157529696
14-15	0.0031870013558260002
16-17	0.011100487773258358
18-19	0.0053206717550654415
20-21	0.013045098770033545
22-23	0.009804080442074899
24-25	0.01380133637989056
26-27	0.010398267135533984
28-29	0.015475862516002527
30-31	0.022687128295710512
32-33	0.014665607934012868
34-35	0.02276815375390948
36-37	0.016259108611925865
38-39	0.02344336590556753
40-41	0.011856725383115375
42-43	0.010776385940462494
44-45	0.018716880843961173
46-47	0.01836577052509899
48-49	0.023497382877700174
50-51	0.016232100125859548
52-53	0.024334645945756156
54-55	0.018446795983297952
56-57	0.02317328104490431
58-59	0.018716880843961173
60-61	0.02157978036699131
62-63	0.024874815667082596
64-65	0.020742517298935323
66-67	0.014233472156951714
68-69	0.02679241817779146
70-71	0.016988337735716563
72-73	0.0200943136333436
74-75	0.021390720964527054
76-77	0.0148276588504108
78-79	0.031167792920535634
80-81	0.0225520858653789
82-83	0.01031724167733502
84-85	0.01512475219714034
86-87	0.014125438212686425
88-89	0.02266011980964419
90-91	0.014584582475813901
92-93	0.00953399558141168
94-95	0.00861570705515673
96-97	0.003403069244356577
98-99	0.002106661913173119
100-101	0.01350424303316102
102-103	0.004132298368147272
104-105	0.002754865578764848
106-107	4.591442631274747E-4
108-109	0.021633797339123954
110-111	0.0010533309565865594
112-113	0.01020920773306973
114-115	0.0024847807181016274
116-117	0.00926391072074846
118-119	0.011721682952783766
120-121	0.004645459603407391
122-123	8.10254581989661E-4
124-125	0.024820798694949953
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1851270.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.14295297536701
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.45790646581287	43.26400646790062
2	10.936723587045966	12.061664689301258
3	3.8276611502984905	6.332056164496465
4	1.8796190658589678	4.145909830410574
5	1.1377949773782587	3.1370687466589047
6	0.7316871076547335	2.420843266205236
7	0.523328842088848	2.0200528410971024
8	0.38925640143410667	1.7171797951713221
9	0.2974950310371508	1.4764279056198264
>10	1.6953003365696446	16.650271741827684
>50	0.09235087040006813	3.439703647477065
>100	0.030089723490861774	2.803410507254972
>500	4.915255813341005E-4	0.1699973668949908
>1k	2.9491534880046017E-4	0.3614070296839615
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2863	0.15465059121576	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2521	0.13617678674639572	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.0803394426528815E-4
7	0.0	0.0	0.0	0.0	1.0803394426528815E-4
8	0.0	0.0	0.0	0.0	1.0803394426528815E-4
9	0.0	0.0	0.0	0.0	1.0803394426528815E-4
10-11	0.0	0.0	0.0	0.0	2.160678885305763E-4
12-13	0.0	0.0	0.0	5.401697213264408E-5	3.7811880492850854E-4
14-15	0.0	0.0	0.0	5.401697213264408E-5	4.5914426312747465E-4
16-17	0.0	0.0	0.0	5.401697213264408E-5	5.401697213264408E-4
18-19	0.0	0.0	0.0	5.401697213264408E-5	5.941866934590849E-4
20-21	0.0	0.0	0.0	5.401697213264408E-5	7.29229123790695E-4
22-23	0.0	0.0	0.0	1.0803394426528815E-4	7.832460959233392E-4
24-25	0.0	0.0	0.0	1.350424303316102E-4	8.102545819896612E-4
26-27	0.0	0.0	0.0	4.5914426312747465E-4	8.102545819896612E-4
28-29	0.0	0.0	0.0	0.0016745261361119663	8.102545819896612E-4
30-31	0.0	0.0	0.0	0.005509731157529696	8.372630680559831E-4
32-33	0.0	0.0	0.0	0.013504243033161018	8.642715541223052E-4
34-35	0.0	0.0	0.0	0.023551399849832816	8.642715541223052E-4
36-37	0.0	0.0	0.0	0.03862213507484051	8.642715541223052E-4
38-39	0.0	0.0	0.0	0.06328088285339253	8.642715541223052E-4
40-41	0.0	0.0	0.0	0.09050543680824516	8.642715541223052E-4
42-43	0.0	0.0	0.0	0.11678469375077649	8.642715541223052E-4
44-45	0.0	0.0	0.0	0.14881675822543444	8.642715541223052E-4
46-47	0.0	0.0	0.0	0.18560231624776505	8.642715541223052E-4
48-49	0.0	0.0	0.0	0.2263311132357787	0.0010533309565865594
50-51	0.0	0.0	0.0	0.2695716994279602	0.0010803394426528816
52-53	0.0	0.0	0.0	0.31200203103815216	0.0011883733869181698
54-55	0.0	0.0	0.0	0.3602661956386697	0.001296407331183458
56-57	0.0	0.0	0.0	0.4136889810778547	0.001296407331183458
58-59	0.0	0.0	0.0	0.4728375655630999	0.001377432789382424
60-61	0.0	0.0	0.0	0.5404668146731704	0.001404441275448746
62-63	0.0	0.0	0.0	0.6090953777676946	0.001404441275448746
64-65	0.0	0.0	0.0	0.6813970949672388	0.001404441275448746
66-67	0.0	0.0	0.0	0.7625575955965365	0.001431449761515068
68-69	0.0	0.0	0.0	0.8517396165875317	0.001485466733647712
70-71	0.0	0.0	0.0	0.9582880941191723	0.0016475176500456443
72-73	0.0	0.0	0.0	1.0671052844803839	0.0018905940246425426
74-75	0.0	0.0	0.0	1.1778670858383704	0.0019446109967751867
76-77	0.0	0.0	0.0	1.3012688586753958	0.001998627968907831
78-79	0.0	0.0	0.0	1.4434145208424487	0.001998627968907831
80-81	0.0	0.0	0.0	1.6025215122591518	0.002025636454974153
82-83	0.0	0.0	0.0	1.7739983902942305	0.002106661913173119
84-85	0.0	0.0	0.0	1.9499046600441858	0.002106661913173119
86-87	0.0	0.0	0.0	2.1384509012731803	0.002160678885305763
88-89	0.0	0.0	0.0	2.348711965299497	0.002214695857438407
90-91	0.0	0.0	0.0	2.5807148606092034	0.002214695857438407
92-93	0.0	0.0	0.0	2.829922161543157	0.002268712829571051
94-95	0.0	0.0	0.0	3.0943082316463832	0.0023497382877700175
96-97	0.0	0.0	0.0	3.3722525617549035	0.0025387976902342714
98-99	0.0	0.0	0.0	3.6750176905583736	0.002754865578764848
100-101	0.0	0.0	0.0	4.017080166588342	0.002754865578764848
102-103	0.0	0.0	0.0	4.3865022390034945	0.00278187406483117
104-105	0.0	0.0	0.0	4.766781722817309	0.002808882550897492
106-107	0.0	0.0	0.0	5.164184586797171	0.002862899523030136
108-109	0.0	0.0	0.0	5.581924840784975	0.003024950439428068
110-111	0.0	5.401697213264408E-5	0.0	6.041312180287046	0.0031870013558260007
112-113	0.0	5.401697213264408E-5	0.0	6.52352169051516	0.0032410183279586446
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2730	0.0	83.43528	1
GTATCAA	5585	0.0	58.186516	1
TCAACGC	7285	0.0	44.46508	4
CAACGCA	7415	0.0	43.57694	5
TATCAAC	7630	0.0	42.534878	2
ATCAACG	7710	0.0	42.014027	3
AACGCAG	7735	0.0	41.765118	6
ACGCAGA	8885	0.0	36.35742	7
GTGGTAT	1075	0.0	34.390003	1
CGCAGAG	9470	0.0	33.79738	8
GTACATG	9290	0.0	33.69711	1
TACATGG	9765	0.0	31.710592	2
ACATGGG	9825	0.0	31.394022	3
GCAGAGT	10110	0.0	31.363663	9
CATGGGG	8145	0.0	29.24278	4
CAGAGTA	9765	0.0	27.598312	10-11
GAGTACT	4940	0.0	27.217598	12-13
AGAGTAC	9165	0.0	27.003338	10-11
GTATAGG	620	0.0	26.9287	1
TGGTATC	1285	0.0	24.560995	2
>>END_MODULE