FastQCFastQC Report
Mon 15 Aug 2016
SRR2921511_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921511_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2176154
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22900.067.578021
GTATCAA47150.061.6035461
TCAACGC59300.047.361464
CAACGCA60350.046.537445
ATCAACG64000.044.0652433
AACGCAG64150.044.0539476
TATCAAC68650.041.947172
GTACATG116150.038.7409441
ACGCAGA76300.037.0370987
TACATGG120900.036.4169242
CGCAGAG81600.034.629918
ACATGGG127450.034.2652633
CATGGGG107600.031.9081294
GCAGAGT91050.029.9909789
ATGGGGG57100.028.7614485
AGAGTAC81700.025.55924210-11
CAGAGTA86450.024.77424210-11
ATGGGGA41650.023.1439655
GTATATA6800.021.882591
GTGGTAT11350.021.5008491
Produced by FastQC (version 0.11.5)