##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921511_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2176154
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.831629562981295	31.0	30.0	34.0	25.0	34.0
2	29.937605518727075	31.0	30.0	34.0	25.0	34.0
3	30.030088863196262	31.0	30.0	34.0	25.0	34.0
4	33.4157550430714	37.0	35.0	37.0	27.0	37.0
5	33.43346059148387	37.0	35.0	37.0	27.0	37.0
6	33.4546254538971	37.0	35.0	37.0	27.0	37.0
7	33.304128751917375	37.0	35.0	37.0	26.0	37.0
8	33.24566965389398	37.0	35.0	37.0	26.0	37.0
9	34.71938061368819	39.0	35.0	39.0	27.0	39.0
10-11	34.59648903524291	39.0	35.0	39.0	26.0	39.0
12-13	34.440306614329685	39.0	35.0	39.0	25.0	39.0
14-15	35.59126008545351	40.0	36.0	41.0	25.0	41.0
16-17	35.50893411036168	40.0	36.0	41.0	25.0	41.0
18-19	35.51827926700041	40.0	36.0	41.0	25.0	41.0
20-21	35.534946745496875	40.0	36.0	41.0	25.0	41.0
22-23	35.4985786851482	40.0	36.0	41.0	25.0	41.0
24-25	35.4519149380053	40.0	35.5	41.0	25.0	41.0
26-27	35.36692784609913	40.0	35.0	41.0	25.0	41.0
28-29	35.31126588467544	40.0	35.0	41.0	24.5	41.0
30-31	35.20971815413799	40.0	35.0	41.0	24.0	41.0
32-33	35.10467434749563	40.0	35.0	41.0	24.0	41.0
34-35	34.93624026608411	40.0	35.0	41.0	23.5	41.0
36-37	34.797205528652846	39.0	34.5	41.0	23.0	41.0
38-39	34.67192165627984	39.0	34.0	41.0	22.0	41.0
40-41	34.52041376667276	39.0	34.0	41.0	21.0	41.0
42-43	34.32890043627427	39.0	34.0	41.0	19.5	41.0
44-45	34.13953240441623	39.0	33.0	41.0	18.0	41.0
46-47	33.892850873605454	39.0	33.0	41.0	15.0	41.0
48-49	33.74716541200669	38.0	33.0	41.0	14.5	41.0
50-51	33.54978576883805	38.0	33.0	40.0	13.0	41.0
52-53	33.35610416358401	38.0	33.0	40.0	10.5	41.0
54-55	33.14490380735923	38.0	33.0	40.0	8.5	41.0
56-57	32.900497391269184	37.0	32.0	40.0	8.0	41.0
58-59	32.64699671990125	37.0	32.0	40.0	7.0	41.0
60-61	32.33740994433298	36.0	32.0	40.0	6.0	41.0
62-63	32.019377994388265	36.0	31.0	39.0	2.0	41.0
64-65	31.70421233975169	35.0	31.0	39.0	2.0	41.0
66-67	31.651389561584338	35.0	31.0	39.0	2.0	41.0
68-69	31.407006351572548	35.0	31.0	38.5	2.0	40.0
70-71	31.105392587105506	35.0	31.0	37.0	2.0	40.0
72-73	30.777141691258983	35.0	31.0	37.0	2.0	39.0
74-75	30.477809704644066	35.0	31.0	36.0	2.0	39.0
76-77	30.20708621724382	35.0	31.0	36.0	2.0	38.5
78-79	29.93651736044416	35.0	30.0	35.5	2.0	37.0
80-81	29.707827892695093	35.0	30.0	35.0	2.0	37.0
82-83	29.51598554146444	35.0	30.0	35.0	2.0	36.5
84-85	29.32230048976313	35.0	30.0	35.0	2.0	36.0
86-87	29.17653438129838	34.5	30.0	35.0	2.0	36.0
88-89	29.056047733754134	34.0	30.0	35.0	2.0	35.5
90-91	28.950257196871178	34.0	30.0	35.0	2.0	35.0
92-93	28.850853156532118	34.0	30.0	35.0	2.0	35.0
94-95	28.78994317497751	34.0	29.0	35.0	2.0	35.0
96-97	28.691299650668107	34.0	29.0	35.0	2.0	35.0
98-99	28.630930991097138	34.0	29.0	35.0	2.0	35.0
100-101	28.565196902425104	34.0	29.0	35.0	2.0	35.0
102-103	27.59083088788753	32.5	27.5	34.5	2.0	35.0
104-105	28.40824339637728	34.0	29.0	35.0	2.0	35.0
106-107	28.728009598585395	34.5	30.0	35.0	2.0	35.0
108-109	28.764960568048032	35.0	30.0	35.0	2.0	35.0
110-111	28.70224625646898	35.0	30.0	35.0	2.0	35.0
112-113	28.62885921676499	35.0	30.0	35.0	2.0	35.0
114-115	28.507396765118642	35.0	30.0	35.0	2.0	35.0
116-117	28.44631055522725	35.0	30.0	35.0	2.0	35.0
118-119	28.286364613901405	34.5	29.5	35.0	2.0	35.0
120-121	28.10972132486947	34.0	29.0	35.0	2.0	35.0
122-123	27.97824464628882	34.0	29.0	35.0	2.0	35.0
124-125	27.129575618269662	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	86021.0
3	15372.0
4	9988.0
5	8309.0
6	9067.0
7	10240.0
8	11266.0
9	12135.0
10	12442.0
11	13060.0
12	13270.0
13	13853.0
14	15123.0
15	12685.0
16	9372.0
17	8312.0
18	7548.0
19	7236.0
20	7043.0
21	7614.0
22	8781.0
23	12072.0
24	16592.0
25	22430.0
26	27112.0
27	29063.0
28	34263.0
29	41262.0
30	46627.0
31	56088.0
32	70931.0
33	90927.0
34	127346.0
35	199272.0
36	382378.0
37	566541.0
38	154396.0
39	117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.884302611557786	16.882312108967376	12.259427305282417	24.97395797419242
2	16.635839213072558	19.11622348371676	38.60311185677185	25.64482544643883
3	19.43217561215076	24.00050193811544	28.582112157428384	27.985210292305407
4	13.48612521438379	14.990569641462503	35.314911458517685	36.20839368563602
5	14.538944496082978	37.024599862644564	33.21055007451579	15.225905566756673
6	33.866086284094145	34.25103109395955	17.225188346190112	14.6576942757562
7	29.99771981490046	30.849203452273795	20.63990452644325	18.513172206382496
8	27.787027902687665	33.50211874340909	19.419669330763934	19.29118402313931
9	27.870136532420357	14.608812689213377	18.725148865478193	38.79590191288807
10-11	26.542560624894268	24.703127413411398	26.778385395597205	21.975926566097133
12-13	26.70392486760812	22.40688897469844	26.87725250073551	24.01193365695793
14-15	24.188433251769453	23.51696775430256	24.21810607840154	28.07649291552645
16-17	24.450138623219285	26.487079378169938	24.71929430185246	24.343487696758316
18-19	24.529031000392177	25.65078203267347	26.2508407902946	23.569346176639755
20-21	25.569503786116165	24.679692917194686	25.894041166311382	23.856762130377763
22-23	25.405258771803783	24.287525503606425	25.67733555561941	24.629880168970374
24-25	24.82969017061102	24.863717649179453	25.711622637102	24.594969543107528
26-27	24.460495987152637	24.81427664728114	25.967822281193325	24.757405084372895
28-29	24.51644878701862	24.89885308594637	25.952551063100042	24.632147063934966
30-31	24.83405595857209	24.86230811121831	25.719849447519845	24.583786482689753
32-33	24.439613533233743	24.9501007640411	25.727062099432473	24.883223603292684
34-35	24.73686097764146	24.99560975250957	25.75480077010917	24.512728499739804
36-37	24.93667222453768	24.69221172385113	25.61212251898684	24.758993532624352
38-39	24.81124701121942	24.953535957329407	25.628471583593893	24.606745447857275
40-41	24.865498581654844	24.854901221569882	25.77592905055929	24.503671146215982
42-43	24.824989459135242	24.834760162566113	25.590506830526344	24.7497435477723
44-45	24.653225682231845	24.89496476908572	25.630527496987742	24.82128205169469
46-47	24.797066482367672	24.92046128088721	25.528606579124645	24.753865657620473
48-49	24.88028576479299	25.157402582117268	25.166735591477167	24.79557606161257
50-51	24.839990933470897	25.26607642274853	25.168146874093406	24.72578576968717
52-53	24.93085032886758	25.05918329013802	25.266511603419424	24.743454777574975
54-55	24.81561400278467	25.094379655049632	25.363191854075477	24.72681448809022
56-57	24.728629532591352	25.159355873559818	25.259762141963616	24.852252451885214
58-59	24.878806790819453	24.93450429109817	25.219129321412275	24.967559596670103
60-61	24.81445276811348	25.1704120112743	25.423952813967336	24.59118240664488
62-63	24.608396007978158	25.21038981219329	25.38920190135246	24.792012278476093
64-65	24.722474178272098	24.91310006468362	25.440453143913466	24.92397261313082
66-67	24.625712838997163	25.121077187612123	25.333983644610097	24.91922632878062
68-69	24.697123199011525	25.172491303036765	25.337857715475074	24.792527782476633
70-71	24.641051423127834	24.886105176335256	25.51844040986964	24.95440299066727
72-73	24.84425611018244	25.177727882528945	25.241645795472333	24.736370211816286
74-75	24.60479216578835	25.303805786796595	25.265810669387545	24.825591378027514
76-77	24.710235247906333	24.95425613850329	25.295223446783012	25.04028516680737
78-79	24.61801256440389	25.150343736403556	25.381401344974545	24.850242354218004
80-81	24.650961936011278	25.27739071739965	25.217406892348336	24.85424045424073
82-83	24.833305641904555	25.170773385986116	25.176265710864254	24.819655261245074
84-85	24.555066093468238	25.21173786719902	25.15780230629102	25.07539373304172
86-87	24.53647508264879	25.246889266481382	25.28393357211437	24.932702078755458
88-89	24.586960808331888	25.11728727303815	25.215534180501287	25.080217738128667
90-91	24.460573828391954	25.140299750399492	25.251427355498496	25.147699065710054
92-93	24.58824710895684	25.26280278076975	25.293137447985814	24.855812662287597
94-95	24.572511251518108	24.994835274557722	25.37977105157027	25.052882422353896
96-97	24.563746575819362	24.969535391453274	25.38936446793731	25.077353564790062
98-99	24.49100818077347	25.108386458994218	25.177967038383525	25.222638321848784
100-101	24.452516250273167	25.089934608261967	25.330158535383163	25.127390606081708
102-103	24.5267723461338	24.84607176240594	25.520561908149485	25.10659398331077
104-105	24.31168280895316	25.081324173770064	25.315973453289697	25.29101956398708
106-107	24.32021055635712	25.116368739256128	25.31761421852851	25.245806485858242
108-109	24.372066687868934	25.034469002715237	25.289815374829722	25.303648934586104
110-111	24.404855594344777	25.10062589455771	25.318525159977767	25.175993351119747
112-113	24.386843124166322	25.08825364291139	25.416267898606254	25.108635334316038
114-115	24.137839913343957	25.32122618119183	25.3735479390926	25.167385966371608
116-117	24.04722081609309	25.381946925754928	25.30389048292742	25.26694177522456
118-119	24.29141834255691	25.065434113076414	25.316161235550833	25.326986308815847
120-121	23.979872888928035	25.150334218233244	25.53181039545641	25.33798249738231
122-123	23.97595619824967	25.211448523221584	25.44313924495163	25.369456033577116
124-125	23.96609484069906	25.292086192080216	25.421470816655717	25.32034815056501
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	150.0
1	184.5
2	233.5
3	234.5
4	205.5
5	192.5
6	195.0
7	231.5
8	292.5
9	331.0
10	353.0
11	391.0
12	459.5
13	529.5
14	613.0
15	701.5
16	795.5
17	918.0
18	1022.5
19	1128.5
20	1285.0
21	1451.5
22	1647.5
23	1912.5
24	2270.0
25	2988.5
26	4154.0
27	5376.0
28	6511.5
29	8131.5
30	10605.5
31	13340.0
32	16549.5
33	19821.5
34	23009.0
35	26319.5
36	30835.0
37	35486.0
38	38533.0
39	42055.5
40	45750.5
41	48631.5
42	51530.5
43	57139.0
44	63640.0
45	69013.0
46	74721.5
47	80434.5
48	86331.5
49	91617.0
50	92529.5
51	93200.5
52	93140.5
53	92626.0
54	93636.0
55	92308.0
56	87691.0
57	80647.0
58	75697.5
59	70202.0
60	62484.5
61	55513.0
62	48193.5
63	37813.0
64	28433.0
65	22732.5
66	17542.0
67	13333.5
68	10749.0
69	8405.0
70	6869.0
71	5567.5
72	4291.0
73	3676.5
74	2938.5
75	2166.5
76	1465.5
77	811.0
78	445.0
79	267.0
80	152.5
81	81.0
82	51.5
83	35.0
84	33.0
85	26.5
86	17.5
87	15.5
88	13.0
89	12.5
90	14.0
91	10.0
92	8.5
93	13.0
94	12.0
95	8.0
96	8.5
97	8.5
98	8.5
99	10.0
100	33.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03786496727713204
2	0.02775538863517931
3	0.026974193921937513
4	0.03368327792977886
5	0.03565924102797872
6	0.047652877507749915
7	0.040989746130099244
8	0.037129725194080934
9	0.03216684113348595
10-11	0.03722163045446232
12-13	0.036486388371411216
14-15	0.03506185683549969
16-17	0.03827854094884829
18-19	0.051329087923005445
20-21	0.06277129284048831
22-23	0.05590137462697952
24-25	0.06621774010479038
26-27	0.04951395903047302
28-29	0.05054789320976365
30-31	0.05047896426447761
32-33	0.05840579297237236
34-35	0.04034640930742953
36-37	0.053328027336300654
38-39	0.06221986127819998
40-41	0.04981265112671254
42-43	0.05904912979504208
44-45	0.059209964000709506
46-47	0.06557440328212066
48-49	0.04955991166066372
50-51	0.05151289844376823
52-53	0.06169140603100699
54-55	0.0473082327813197
56-57	0.04553905651897797
58-59	0.04648108543788721
60-61	0.03513078578078573
62-63	0.044367264449115276
64-65	0.043700951311350204
66-67	0.03609579101479032
68-69	0.03133969379005346
70-71	0.05151289844376823
72-73	0.032511485859916166
74-75	0.0401855751017621
76-77	0.033338633203348664
78-79	0.03320077531277658
80-81	0.02607811763321897
82-83	0.017944502089466095
84-85	0.018817602063089287
86-87	0.0175079521026545
88-89	0.023573699287826138
90-91	0.012728878562822298
92-93	0.019552844146140395
94-95	0.015463060059168606
96-97	0.0123382812062014
98-99	0.01192470753448515
100-101	0.012292328576010706
102-103	0.0064333682266971915
104-105	0.006456344541792539
106-107	0.009397312873996968
108-109	0.013142452234538547
110-111	0.006823965583318093
112-113	0.008340402379611003
114-115	0.008960762887185373
116-117	0.0075592076663692
118-119	0.24207845584457718
120-121	0.3554665708401152
122-123	0.028697417554088545
124-125	0.003860020936018315
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2176154.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.72553541925726
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.38885072078949	42.11482975874646
2	10.875435460636183	11.685771860805113
3	3.781407945061302	6.0947449946115535
4	1.9109642926861288	4.106703191665781
5	1.1243649984332766	3.0203555773750064
6	0.7745350368179017	2.4967385732409544
7	0.53369905480262	2.00712872304156
8	0.4014722616900302	1.725544977222163
9	0.305495007049547	1.4771594539482016
>10	1.7551992972268522	17.13643165531759
>50	0.10719057103174345	3.9000427323516518
>100	0.040351506958440725	3.7647853436102796
>500	7.753851123843235E-4	0.26522247469743254
>1k	2.5846170412809777E-4	0.2045406833661286
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.595263019069423E-5	4.595263019069423E-5
5	0.0	0.0	0.0	4.595263019069423E-5	4.595263019069423E-5
6	0.0	0.0	0.0	4.595263019069423E-5	4.595263019069423E-5
7	0.0	0.0	0.0	4.595263019069423E-5	4.595263019069423E-5
8	0.0	0.0	0.0	4.595263019069423E-5	4.595263019069423E-5
9	0.0	0.0	0.0	4.595263019069423E-5	4.595263019069423E-5
10-11	0.0	0.0	0.0	4.595263019069423E-5	4.595263019069423E-5
12-13	0.0	0.0	0.0	4.595263019069423E-5	4.595263019069423E-5
14-15	0.0	0.0	0.0	9.190526038138846E-5	9.190526038138846E-5
16-17	0.0	0.0	0.0	9.190526038138846E-5	1.3785789057208268E-4
18-19	0.0	0.0	0.0	9.190526038138846E-5	1.3785789057208268E-4
20-21	0.0	0.0	0.0	9.190526038138846E-5	1.3785789057208268E-4
22-23	0.0	0.0	0.0	1.3785789057208268E-4	2.0678683585812402E-4
24-25	0.0	0.0	0.0	2.2976315095347112E-4	2.7571578114416536E-4
26-27	0.0	0.0	0.0	2.527394660488182E-4	2.7571578114416536E-4
28-29	0.0	0.0	0.0	7.811947132418018E-4	2.7571578114416536E-4
30-31	0.0	0.0	0.0	0.003469423579397414	2.986920962395125E-4
32-33	0.0	0.0	0.0	0.008547189215469125	3.676210415255538E-4
34-35	0.0	0.0	0.0	0.01583068110069416	4.1357367171624803E-4
36-37	0.0	0.0	0.0	0.026353833414363138	4.1357367171624803E-4
38-39	0.0	0.0	0.0	0.043103567118871186	5.744078773836778E-4
40-41	0.0	0.0	0.0	0.06201307444234186	5.97384192479025E-4
42-43	0.0	0.0	0.0	0.08147401332810086	6.20360507574372E-4
44-45	0.0	0.0	0.0	0.10226757848939	6.433368226697191E-4
46-47	0.0	0.0	0.0	0.1271968803678416	6.433368226697191E-4
48-49	0.0	0.0	0.0	0.15463060059168604	7.122657679557605E-4
50-51	0.0	0.0	0.0	0.18433897600996987	7.352420830511076E-4
52-53	0.0	0.0	0.0	0.21232412779610266	7.811947132418018E-4
54-55	0.0	0.0	0.0	0.24370977421634682	8.271473434324961E-4
56-57	0.0	0.0	0.0	0.28293034408410433	8.271473434324961E-4
58-59	0.0	0.0	0.0	0.32784904009550797	8.271473434324961E-4
60-61	0.0	0.0	0.0	0.37609930179573686	8.271473434324961E-4
62-63	0.0	0.0	0.0	0.42508480557901696	8.271473434324961E-4
64-65	0.0	0.0	0.0	0.4766436566529758	8.271473434324961E-4
66-67	0.0	0.0	0.0	0.537553867970741	8.501236585278431E-4
68-69	0.0	0.0	0.0	0.6066206711473545	8.960762887185373E-4
70-71	0.0	0.0	0.0	0.6876581344886437	9.190526038138845E-4
72-73	0.0	0.0	0.0	0.7670183268279727	0.0012866736453394383
74-75	0.0	0.0	0.0	0.8481476954296433	0.0013326262755301325
76-77	0.0	0.0	0.0	0.9416383215526107	0.0013326262755301325
78-79	0.0	0.0	0.0	1.0474212762515889	0.0013326262755301325
80-81	0.0	0.0	0.0	1.1670589489530614	0.001424531535911521
82-83	0.0	0.0	0.0	1.29604798189834	0.0015164367962929093
84-85	0.0	0.0	0.0	1.4312635962344578	0.0015164367962929093
86-87	0.0	0.0	0.0	1.5735788919350377	0.0015394131113882566
88-89	0.0	0.0	4.595263019069423E-5	1.7303692661456864	0.0015853657415789506
90-91	0.0	0.0	4.595263019069423E-5	1.9084127318195312	0.001631318371769645
92-93	0.0	0.0	4.595263019069423E-5	2.1018503286072585	0.0016542946868649921
94-95	4.595263019069423E-5	0.0	4.595263019069423E-5	2.2988033016045737	0.0017461999472463807
96-97	4.595263019069423E-5	0.0	4.595263019069423E-5	2.508186461068472	0.0018610815227231162
98-99	4.595263019069423E-5	0.0	4.595263019069423E-5	2.737926635706848	0.0019300104680091575
100-101	4.595263019069423E-5	0.0	4.595263019069423E-5	3.000913538288191	0.0019300104680091575
102-103	4.595263019069423E-5	0.0	4.595263019069423E-5	3.2821895876854303	0.0020448920434858932
104-105	4.595263019069423E-5	0.0	4.595263019069423E-5	3.569646265843318	0.0020908446736765873
106-107	4.595263019069423E-5	0.0	4.595263019069423E-5	3.8753231618718162	0.0021367973038672813
108-109	4.595263019069423E-5	0.0	4.595263019069423E-5	4.198393128427492	0.0021827499340579754
110-111	4.595263019069423E-5	0.0	4.595263019069423E-5	4.55202159405998	0.0022976315095347114
112-113	4.595263019069423E-5	0.0	4.595263019069423E-5	4.9306712668312995	0.0023895367699161
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2290	0.0	67.57802	1
GTATCAA	4715	0.0	61.603546	1
TCAACGC	5930	0.0	47.36146	4
CAACGCA	6035	0.0	46.53744	5
ATCAACG	6400	0.0	44.065243	3
AACGCAG	6415	0.0	44.053947	6
TATCAAC	6865	0.0	41.94717	2
GTACATG	11615	0.0	38.740944	1
ACGCAGA	7630	0.0	37.037098	7
TACATGG	12090	0.0	36.416924	2
CGCAGAG	8160	0.0	34.62991	8
ACATGGG	12745	0.0	34.265263	3
CATGGGG	10760	0.0	31.908129	4
GCAGAGT	9105	0.0	29.990978	9
ATGGGGG	5710	0.0	28.761448	5
AGAGTAC	8170	0.0	25.559242	10-11
CAGAGTA	8645	0.0	24.774242	10-11
ATGGGGA	4165	0.0	23.143965	5
GTATATA	680	0.0	21.88259	1
GTGGTAT	1135	0.0	21.500849	1
>>END_MODULE