FastQCFastQC Report
Mon 15 Aug 2016
SRR2921511_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921511_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2176154
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30200.13877694317589656No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26790.12310709628086984No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27200.076.936571
GTATCAA58050.062.136641
TCAACGC76450.046.5084234
CAACGCA79400.044.5955665
ATCAACG81850.043.5128253
TATCAAC82000.043.433232
AACGCAG83500.042.6801386
ACGCAGA96650.036.6842737
CGCAGAG102850.034.471298
GTACATG110800.034.383951
TACATGG113600.033.1339762
GCAGAGT110550.031.3707839
ACATGGG122000.030.7549863
CATGGGG102500.028.2969674
AGAGTAC102850.026.72133810-11
CAGAGTA107650.025.58512510-11
ATGGGGG51000.025.0878525
GTATAGA6150.024.2359181
GTATAGG6500.023.8481451
GAGTACT54800.023.177312-13
Produced by FastQC (version 0.11.5)