##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921511_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2176154
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.179260291321295	31.0	31.0	34.0	28.0	34.0
2	31.232530877869856	33.0	31.0	34.0	28.0	34.0
3	31.318672299846426	33.0	31.0	34.0	28.0	34.0
4	34.83502959808911	37.0	35.0	37.0	32.0	37.0
5	34.480414529486424	37.0	35.0	37.0	30.0	37.0
6	34.45711562692714	37.0	35.0	37.0	30.0	37.0
7	34.29185572344604	37.0	35.0	37.0	30.0	37.0
8	34.25584678290231	37.0	35.0	37.0	30.0	37.0
9	35.8305933311705	39.0	37.0	39.0	30.0	39.0
10-11	35.69647575493279	39.0	35.0	39.0	30.0	39.0
12-13	35.600482318806485	39.0	35.0	39.0	30.0	39.0
14-15	36.76355143064323	40.0	37.0	41.0	30.0	41.0
16-17	36.739850672332935	40.0	37.0	41.0	30.0	41.0
18-19	36.749421226622744	40.0	37.0	41.0	30.0	41.0
20-21	36.73187743146855	40.0	37.0	41.0	30.0	41.0
22-23	36.671806774704365	40.0	37.0	41.0	30.0	41.0
24-25	36.605796510724886	40.0	36.0	41.0	29.5	41.0
26-27	36.48365143275706	40.0	36.0	41.0	29.0	41.0
28-29	36.29562292006908	40.0	36.0	41.0	28.5	41.0
30-31	36.25584609361286	40.0	36.0	41.0	29.0	41.0
32-33	36.14474894699548	40.0	35.5	41.0	28.5	41.0
34-35	36.02232838300966	40.0	35.0	41.0	28.0	41.0
36-37	35.89703669868952	40.0	35.0	41.0	27.0	41.0
38-39	35.7299995772358	40.0	35.0	41.0	27.0	41.0
40-41	35.52897635001935	39.5	35.0	41.0	26.0	41.0
42-43	35.3630285356643	39.0	35.0	41.0	26.0	41.0
44-45	35.15480016579709	39.0	35.0	41.0	24.5	41.0
46-47	34.90089258388882	39.0	34.5	41.0	24.0	41.0
48-49	34.657787776048934	39.0	34.0	41.0	23.0	41.0
50-51	34.440135670545374	39.0	34.0	41.0	23.0	41.0
52-53	34.237559933717925	38.0	34.0	40.5	22.0	41.0
54-55	34.02349098455348	38.0	33.5	40.0	22.0	41.0
56-57	33.75792567989214	37.5	33.0	40.0	21.0	41.0
58-59	33.61076077336439	37.0	33.0	40.0	20.5	41.0
60-61	33.48673738163751	37.0	33.0	40.0	21.0	41.0
62-63	33.21403149777084	36.0	33.0	40.0	20.0	41.0
64-65	32.916369429737045	36.0	33.0	39.0	20.0	41.0
66-67	32.60929281659294	35.0	33.0	39.0	19.0	41.0
68-69	32.291485345246706	35.0	32.0	38.5	18.0	40.5
70-71	31.99070768888599	35.0	32.0	37.5	18.0	40.0
72-73	31.67182079025657	35.0	32.0	37.0	16.0	39.0
74-75	31.382997021350512	35.0	32.0	36.5	14.5	39.0
76-77	31.096169204936782	35.0	32.0	36.0	12.5	38.5
78-79	30.820662278496833	35.0	31.0	35.5	10.5	37.0
80-81	30.605208546821594	35.0	31.0	35.0	9.0	37.0
82-83	30.4197421230299	35.0	31.0	35.0	7.5	36.5
84-85	30.233778951305837	35.0	31.0	35.0	7.0	36.0
86-87	30.10041453867695	35.0	31.0	35.0	7.0	36.0
88-89	29.999963697422146	35.0	31.0	35.0	6.5	35.5
90-91	29.90772895668229	35.0	31.0	35.0	3.5	35.0
92-93	29.971896749954276	35.0	31.0	35.0	2.0	35.0
94-95	29.943207603873624	35.0	31.0	35.0	2.0	35.0
96-97	29.925330652150535	35.0	31.0	35.0	2.0	35.0
98-99	29.888009534251715	35.0	31.0	35.0	2.0	35.0
100-101	29.837428325385062	35.0	31.0	35.0	2.0	35.0
102-103	28.942761633597623	33.5	29.5	35.0	2.0	35.0
104-105	29.654829115954108	34.5	31.0	35.0	2.0	35.0
106-107	29.76789671135407	35.0	31.0	35.0	2.0	35.0
108-109	29.775495897808703	35.0	31.0	35.0	2.0	35.0
110-111	29.7407226234908	35.0	31.0	35.0	2.0	35.0
112-113	29.692391255398284	35.0	31.0	35.0	2.0	35.0
114-115	29.629323338329918	35.0	31.0	35.0	2.0	35.0
116-117	29.552381862680676	35.0	31.0	35.0	2.0	35.0
118-119	29.459081020920394	35.0	31.0	35.0	2.0	35.0
120-121	29.338231577360794	35.0	31.0	35.0	2.0	35.0
122-123	29.217838673182136	35.0	31.0	35.0	2.0	35.0
124-125	28.303921275792064	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	39301.0
3	14057.0
4	7836.0
5	6659.0
6	6945.0
7	8097.0
8	9214.0
9	10268.0
10	11613.0
11	12255.0
12	12714.0
13	14438.0
14	17529.0
15	14576.0
16	8815.0
17	7342.0
18	6713.0
19	6284.0
20	6028.0
21	6544.0
22	7818.0
23	10501.0
24	14503.0
25	20109.0
26	25342.0
27	28074.0
28	32884.0
29	39032.0
30	46297.0
31	55819.0
32	69160.0
33	89351.0
34	123135.0
35	195759.0
36	390281.0
37	618368.0
38	182279.0
39	214.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.98763984822755	17.459306736501258	12.479405498093643	25.07364791717755
2	16.91899696523531	19.538756206011076	37.73512073804889	25.807126090704724
3	19.35118389634386	24.132456662476358	28.867766258896747	27.648593182283037
4	13.56475144477309	15.485981252548958	35.0198100242355	35.929457278442456
5	14.506538576451932	37.04722943623452	33.182459607915234	15.263772379398313
6	33.973014265044114	34.628169371429834	16.643532009479387	14.755284354046665
7	29.84980099090901	31.222458152938255	20.27360302971808	18.654137826434656
8	27.95397445506889	33.55234284417023	19.051621569579904	19.44206113118098
9	27.891327465186794	14.316497567602097	18.63461275104065	39.15756221617046
10-11	26.659813035578754	24.68736435681376	26.532261047975954	22.12056155963153
12-13	26.928206247630428	22.30328236118611	26.958904424351744	23.809606966831723
14-15	24.375872556038892	23.33882333261951	24.450042691484406	27.835261419857193
16-17	24.32990325605721	26.368417460649013	24.920647723227898	24.38103156006588
18-19	24.415087437597915	25.199757905413072	26.950646803390978	23.43450785359803
20-21	25.41765440689168	24.2523813769566	26.741027151787677	23.58893706436405
22-23	25.227857432389577	23.883392878972685	26.53721043309457	24.351539255543166
24-25	24.68534182732114	24.491575220026903	26.531203156888218	24.291879795763744
26-27	24.320865725583335	24.468756376577943	26.72896028000875	24.48141761782997
28-29	24.451185687097365	24.528788364148436	26.687990359527664	24.332035589226532
30-31	24.736756226314498	24.492575764888112	26.513631589712478	24.257036419084915
32-33	24.314368559099677	24.619859631287397	26.45547170036781	24.610300109245113
34-35	24.534800051754637	24.6669904012616	26.524457857546512	24.27375168943725
36-37	24.836846579138285	24.406657905096058	26.269269154943526	24.487226360822127
38-39	24.74001704761036	24.60723045735535	26.25525039051162	24.397502104522673
40-41	24.73038767370957	24.56806418671429	26.361619227336526	24.339928912239614
42-43	24.789849734115275	24.580767970484523	26.072121374502917	24.557260920897285
44-45	24.686772084061822	24.626470539570132	26.034870103487012	24.651887272881034
46-47	24.817156768795982	24.70997637159425	25.783733674178027	24.68913318543174
48-49	24.945855361192542	24.998253398748147	25.35414638540871	24.701744854650602
50-51	24.87171285004065	25.15919556316048	25.288367452085065	24.680724134713802
52-53	25.004303344290296	24.992927347368017	25.355833140094596	24.646936168247088
54-55	24.857399386738656	24.994249094964964	25.39693884093468	24.751412677361703
56-57	24.747461863897485	25.03307614718558	25.385015086216306	24.834446902700627
58-59	24.797529680556277	24.827496344940204	25.445283031008604	24.92969094349491
60-61	24.869903580190964	25.033782324168747	25.571220033699593	24.525094061940695
62-63	24.703022870841348	25.038741642939826	25.55626978018556	24.701965706033263
64-65	24.73380926281488	24.80288912865378	25.652805881210668	24.81049572732067
66-67	24.717968939758997	24.98574707065248	25.538997416433943	24.757286573154584
68-69	24.799142123882294	24.97261107310126	25.458177045424822	24.770069757591624
70-71	24.654368031576475	24.7704864010751	25.734064345157197	24.841081222191224
72-73	24.980742078053876	25.062369928594013	25.292913452416848	24.663974540935264
74-75	24.735022853496094	25.23230959690565	25.285395801317872	24.747271748280387
76-77	24.803426537928676	24.892518546281313	25.33632405500311	24.9677308607869
78-79	24.730706565020803	25.109430803532952	25.345702509559466	24.81416012188678
80-81	24.773574559639577	25.24255846843787	25.130845292534087	24.85302167938847
82-83	24.880171458391306	25.092380400671622	25.15701968108684	24.87042845985023
84-85	24.632943732324506	25.119211566377132	25.095496788968585	25.152347912329777
86-87	24.62612415527498	25.173334080931824	25.236435683243748	24.96410608054945
88-89	24.62440854385675	25.059332338543307	25.175733343567735	25.140525774032213
90-91	24.48773853983883	25.11754070378272	25.260841550075785	25.133879206302662
92-93	24.641071309165703	25.18391354230982	25.260615513789464	24.91439963473501
94-95	24.5857765929164	24.91753675775327	25.35251528111952	25.144171368210817
96-97	24.56932611643193	24.935578424752038	25.367774530115984	25.127320928700048
98-99	24.495715506113452	25.015199188641347	25.174107107981563	25.31497819726364
100-101	24.441456640821222	25.057964973300496	25.317124128457273	25.183454257421012
102-103	24.530784282548694	24.78720891438634	25.451822039467338	25.23018476359763
104-105	24.369329676907185	25.03045043202735	25.248687144644432	25.351532746421025
106-107	24.376024889845525	25.025199337269495	25.225162712930665	25.37361305995432
108-109	24.4704110912503	24.990290467836285	25.131555542890393	25.407742898023024
110-111	24.497279234017583	25.04037551005028	25.187655077227568	25.274690178704567
112-113	24.412515298063493	25.01639529598622	25.301951488306834	25.26913791764345
114-115	24.146024438657847	25.27536054729065	25.226419815435996	25.352195198615508
116-117	24.127450550112123	25.2523534954943	25.17312429815638	25.447071656237195
118-119	24.20071587731507	24.96666370241894	25.228528563514065	25.604091856751925
120-121	23.975212880037887	25.0702936674924	25.406361390216535	25.54813206225318
122-123	24.017692799565467	25.090234334701428	25.28158236179252	25.610490503940582
124-125	23.9687252441315	25.242568729242993	25.195317841507748	25.59338818511776
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	45.0
1	47.0
2	87.5
3	131.0
4	96.0
5	82.5
6	176.5
7	379.5
8	621.0
9	709.0
10	559.5
11	434.5
12	478.0
13	552.0
14	658.5
15	778.0
16	940.0
17	1107.5
18	1238.5
19	1425.0
20	1671.0
21	1894.0
22	2131.0
23	2472.0
24	2853.0
25	3545.0
26	4679.0
27	5875.5
28	6938.5
29	8541.0
30	10991.5
31	13521.5
32	16610.0
33	19690.0
34	22764.0
35	26147.0
36	30369.5
37	34692.0
38	37771.0
39	41230.5
40	44813.0
41	47664.0
42	50702.5
43	56549.0
44	62921.0
45	68259.0
46	74290.0
47	79959.0
48	86114.5
49	92138.5
50	93249.0
51	93473.5
52	93326.0
53	93163.0
54	94593.0
55	93257.5
56	88432.0
57	81099.5
58	75837.0
59	70482.0
60	62501.5
61	55386.0
62	48137.5
63	37791.0
64	28240.0
65	22218.0
66	17255.0
67	13208.0
68	10510.5
69	8123.0
70	6570.0
71	5349.5
72	4150.0
73	3641.0
74	2899.0
75	2069.0
76	1342.0
77	713.5
78	393.0
79	227.0
80	115.5
81	32.5
82	15.5
83	8.5
84	5.5
85	6.0
86	4.0
87	2.0
88	2.0
89	2.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12117708581286066
2	0.0017461999472463804
3	0.02293036246515642
4	0.05721102458741431
5	0.02775538863517931
6	0.022332978272677394
7	0.01718628369131964
8	0.006663131377650663
9	0.005146694581357754
10-11	0.00820254448903892
12-13	0.005927889294599555
14-15	0.003492399894492761
16-17	0.011740897013722374
18-19	0.006663131377650663
20-21	0.014337220619496598
22-23	0.01066101020424106
24-25	0.015646870579931384
26-27	0.010201483902334118
28-29	0.015026510072357013
30-31	0.023389888767063362
32-33	0.01438317324968729
34-35	0.023137149301014542
36-37	0.016749733704508045
38-39	0.022172144067009965
40-41	0.011580062808054944
42-43	0.00969600497023648
44-45	0.01939200994047296
46-47	0.01759985736303589
48-49	0.022976315095347113
50-51	0.017048425800747558
52-53	0.023918344014256344
54-55	0.018817602063089287
56-57	0.022631670368916907
58-59	0.018518909966849774
60-61	0.021138209887719344
62-63	0.024056201904828427
64-65	0.018909507323470673
66-67	0.013119475919443202
68-69	0.025205017659595784
70-71	0.01640508897807784
72-73	0.019575820461235738
74-75	0.020563802010335665
76-77	0.01440614956478264
78-79	0.031822196407055754
80-81	0.02265464668401225
82-83	0.010683986519336407
84-85	0.013579002221350143
86-87	0.01369388379682688
88-89	0.022310001957582044
90-91	0.014337220619496598
92-93	0.009167549723043497
94-95	0.008478260270183084
96-97	0.002688228866155612
98-99	0.0023435841397254054
100-101	0.012453162781678136
102-103	0.003492399894492761
104-105	0.0035153762095881083
106-107	2.527394660488182E-4
108-109	0.02100035199714726
110-111	9.420289189092316E-4
112-113	0.010339341792906201
114-115	0.002412513085011447
116-117	0.008662070790945862
118-119	0.010224460217429465
120-121	0.004802049854927546
122-123	6.663131377650663E-4
124-125	0.024515728206735372
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2176154.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.340075966991186
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.60820097388358	40.620191346345855
2	11.087536062886427	11.606449596364598
3	4.063350032314891	6.380281481155124
4	1.962776693050866	4.109275248820882
5	1.2017655870755948	3.1450251061026195
6	0.7649371835094873	2.4022122176917704
7	0.5579137669662708	2.044087426223138
8	0.43568357356129445	1.8242969070214676
9	0.31599536851018195	1.4885299433736268
>10	1.8424815610514726	17.587403181859067
>50	0.11413655399497781	4.088912334918184
>100	0.044077434901780184	4.065616642352852
>500	7.928365107306799E-4	0.24681566721689668
>1k	3.5237178254693657E-4	0.3909029005538365
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3020	0.13877694317589656	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2679	0.12310709628086984	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.595263019069423E-5	0.0
5	0.0	0.0	0.0	4.595263019069423E-5	0.0
6	0.0	0.0	0.0	4.595263019069423E-5	4.595263019069423E-5
7	0.0	0.0	0.0	4.595263019069423E-5	4.595263019069423E-5
8	0.0	0.0	0.0	4.595263019069423E-5	9.190526038138846E-5
9	0.0	0.0	0.0	4.595263019069423E-5	9.190526038138846E-5
10-11	0.0	0.0	0.0	4.595263019069423E-5	9.190526038138846E-5
12-13	0.0	0.0	0.0	4.595263019069423E-5	1.3785789057208268E-4
14-15	0.0	0.0	0.0	4.595263019069423E-5	2.0678683585812402E-4
16-17	0.0	0.0	0.0	4.595263019069423E-5	2.7571578114416536E-4
18-19	0.0	0.0	0.0	9.190526038138846E-5	3.2166841133485956E-4
20-21	0.0	0.0	0.0	9.190526038138846E-5	3.446447264302067E-4
22-23	0.0	0.0	0.0	1.608342056674298E-4	3.905973566209009E-4
24-25	0.0	0.0	0.0	2.7571578114416536E-4	4.3654998681159516E-4
26-27	0.0	0.0	0.0	2.986920962395125E-4	5.054789320976364E-4
28-29	0.0	0.0	0.0	9.650052340045786E-4	5.514315622883307E-4
30-31	0.0	0.0	0.0	0.003653234100160191	6.20360507574372E-4
32-33	0.0	0.0	0.0	0.008730999736231902	6.433368226697191E-4
34-35	0.0	0.0	0.0	0.016037467936552283	6.892894528604134E-4
36-37	0.0	0.0	0.0	0.026928241291746816	6.892894528604134E-4
38-39	0.0	0.0	0.0	0.04399964340758972	7.352420830511075E-4
40-41	0.0	0.0	0.0	0.06373629807449288	7.811947132418018E-4
42-43	0.0	0.0	0.0	0.08365676326215883	7.811947132418018E-4
44-45	0.0	0.0	0.0	0.10490985472535491	8.041710283371489E-4
46-47	0.0	0.0	0.0	0.13029868290571348	8.730999736231903E-4
48-49	0.0	0.0	0.0	0.15853657415789507	0.001010957864195273
50-51	0.0	0.0	0.0	0.1888882863988486	0.001010957864195273
52-53	0.0	0.0	0.0	0.2171721302812209	0.0011028631245766614
54-55	0.0	0.0	0.0	0.24899432668827665	0.00119476838495805
56-57	0.0	0.0	0.0	0.2886973991730365	0.00119476838495805
58-59	0.0	0.0	0.0	0.3345581241033493	0.00119476838495805
60-61	0.0	0.0	0.0	0.38393422524325027	0.00119476838495805
62-63	0.0	0.0	0.0	0.4339077105756302	0.00119476838495805
64-65	0.0	0.0	0.0	0.4869600221307867	0.00119476838495805
66-67	0.0	0.0	0.0	0.5488582149976517	0.00119476838495805
68-69	0.0	0.0	0.0	0.6192576444497954	0.00119476838495805
70-71	0.0	0.0	0.0	0.7021332129987123	0.00119476838495805
72-73	0.0	0.0	0.0	0.7830787710796203	0.0012866736453394383
74-75	0.0	0.0	0.0	0.8662070790945862	0.0013096499604347855
76-77	0.0	0.0	0.0	0.9608694972874162	0.0013326262755301325
78-79	0.0	0.0	0.0	1.0689271071808337	0.0013326262755301325
80-81	0.0	0.0	0.0	1.1903799087748386	0.001401555220816174
82-83	0.0	0.0	0.0	1.3218274074353193	0.0015164367962929093
84-85	0.0	0.0	0.0	1.4594555348564486	0.0015164367962929093
86-87	0.0	0.0	0.0	1.6042982252175169	0.0015164367962929093
88-89	0.0	0.0	0.0	1.764695880898135	0.0015164367962929093
90-91	0.0	0.0	0.0	1.9469440122344284	0.0015164367962929093
92-93	0.0	0.0	0.0	2.145114729931797	0.0015164367962929093
94-95	0.0	0.0	0.0	2.346594037002896	0.0016542946868649921
96-97	0.0	0.0	0.0	2.559676383197145	0.0016772710019603392
98-99	0.0	0.0	0.0	2.7945632524168786	0.0017232236321510334
100-101	0.0	0.0	0.0	3.0626508969493886	0.0017461999472463804
102-103	0.0	0.0	0.0	3.3491885225034626	0.0017691762623417277
104-105	0.0	0.0	0.0	3.641791895242708	0.0017921525774370747
106-107	4.595263019069423E-5	0.0	0.0	3.952960130578994	0.001838105207627769
108-109	4.595263019069423E-5	0.0	0.0	4.283313589019895	0.0019529867831045045
110-111	4.595263019069423E-5	0.0	0.0	4.646132580690521	0.0021367973038672813
112-113	4.595263019069423E-5	0.0	0.0	5.034455282116983	0.0021597736189626284
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2720	0.0	76.93657	1
GTATCAA	5805	0.0	62.13664	1
TCAACGC	7645	0.0	46.508423	4
CAACGCA	7940	0.0	44.595566	5
ATCAACG	8185	0.0	43.512825	3
TATCAAC	8200	0.0	43.43323	2
AACGCAG	8350	0.0	42.680138	6
ACGCAGA	9665	0.0	36.684273	7
CGCAGAG	10285	0.0	34.47129	8
GTACATG	11080	0.0	34.38395	1
TACATGG	11360	0.0	33.133976	2
GCAGAGT	11055	0.0	31.370783	9
ACATGGG	12200	0.0	30.754986	3
CATGGGG	10250	0.0	28.296967	4
AGAGTAC	10285	0.0	26.721338	10-11
CAGAGTA	10765	0.0	25.585125	10-11
ATGGGGG	5100	0.0	25.087852	5
GTATAGA	615	0.0	24.235918	1
GTATAGG	650	0.0	23.848145	1
GAGTACT	5480	0.0	23.1773	12-13
>>END_MODULE