FastQCFastQC Report
Mon 15 Aug 2016
SRR2921510_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921510_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1977235
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23400.071.481221
GTATCAA44800.055.6720851
TCAACGC55350.043.6438454
CAACGCA57000.042.275015
AACGCAG58900.040.604146
ATCAACG62200.038.45383
TATCAAC65750.037.1910672
GTACATG104400.036.832691
TACATGG110500.034.5134472
ACATGGG109600.034.200593
ACGCAGA71100.033.3841977
CATGGGG82800.031.2588844
CGCAGAG75400.031.1631558
GCAGAGT78650.028.4390939
CAGAGTA76300.025.65095110-11
AGAGTAC72850.024.70176110-11
ATGGGGG44400.023.7188645
CATGGGT21450.022.7457944
ATGGGGA34800.022.568275
GTATTAC4500.022.4873281
Produced by FastQC (version 0.11.5)