##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921510_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1977235
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.131792629606498	31.0	30.0	34.0	23.0	34.0
2	29.26641446262078	31.0	30.0	34.0	22.0	34.0
3	29.345325163675536	31.0	30.0	34.0	22.0	34.0
4	32.695253725530854	37.0	33.0	37.0	24.0	37.0
5	32.71700986478593	37.0	33.0	37.0	25.0	37.0
6	32.71145867840697	37.0	35.0	37.0	24.0	37.0
7	32.52356902442047	37.0	33.0	37.0	22.0	37.0
8	32.46295508626946	37.0	33.0	37.0	21.0	37.0
9	33.86104585443814	39.0	34.0	39.0	19.0	39.0
10-11	33.72972079697153	39.0	34.0	39.0	18.5	39.0
12-13	33.58148146274975	39.0	34.0	39.0	17.0	39.0
14-15	34.64607343082638	40.0	34.0	41.0	17.0	41.0
16-17	34.54461634555326	39.0	34.0	41.0	17.0	41.0
18-19	34.56182471987397	39.5	34.0	41.0	17.5	41.0
20-21	34.57619605155685	40.0	34.0	41.0	18.0	41.0
22-23	34.526841523642865	39.5	34.0	41.0	18.0	41.0
24-25	34.47705507944175	39.0	34.0	41.0	18.0	41.0
26-27	34.38419333058539	39.0	34.0	41.0	17.5	41.0
28-29	34.32097803245441	39.0	34.0	41.0	17.0	41.0
30-31	34.21473775246746	39.0	34.0	41.0	16.0	41.0
32-33	34.103200176003355	39.0	33.0	41.0	16.0	41.0
34-35	33.93581289022296	39.0	33.0	41.0	15.0	41.0
36-37	33.78428689558904	39.0	33.0	41.0	10.5	41.0
38-39	33.6553758152167	39.0	33.0	41.0	9.0	41.0
40-41	33.50287168697702	39.0	33.0	41.0	8.5	41.0
42-43	33.30514860398486	38.5	33.0	41.0	8.0	41.0
44-45	33.112866705272765	38.0	32.0	41.0	4.5	41.0
46-47	32.85574375327161	38.0	32.0	40.0	2.0	41.0
48-49	32.71589694699922	38.0	32.0	40.0	2.0	41.0
50-51	32.524017883559615	38.0	32.0	40.0	2.0	41.0
52-53	32.33813052065131	38.0	31.0	40.0	2.0	41.0
54-55	32.127250175118284	37.0	31.0	40.0	2.0	41.0
56-57	31.89519328759606	37.0	31.0	40.0	2.0	41.0
58-59	31.645900714887205	36.5	31.0	40.0	2.0	41.0
60-61	31.345530753805185	36.0	30.5	39.5	2.0	41.0
62-63	31.036688860959877	35.5	30.0	39.0	2.0	41.0
64-65	30.74109880717264	35.0	30.0	39.0	2.0	40.5
66-67	30.703318017332286	35.0	30.0	39.0	2.0	40.5
68-69	30.46093736960958	35.0	30.0	38.0	2.0	40.0
70-71	30.1701171079816	35.0	30.0	37.0	2.0	40.0
72-73	29.85083664814754	35.0	30.0	37.0	2.0	39.0
74-75	29.555645383578586	35.0	29.0	36.0	2.0	39.0
76-77	29.28823028117548	35.0	29.0	36.0	2.0	38.5
78-79	29.0153676725326	35.0	29.0	35.5	2.0	37.0
80-81	28.774518962085942	34.0	29.0	35.0	2.0	37.0
82-83	28.573509218681643	34.0	29.0	35.0	2.0	36.5
84-85	28.360299357436016	34.0	29.0	35.0	2.0	36.0
86-87	28.20935043128409	34.0	29.0	35.0	2.0	36.0
88-89	28.083267795684378	34.0	29.0	35.0	2.0	35.5
90-91	27.96826452090925	34.0	28.0	35.0	2.0	35.0
92-93	27.869698088492264	34.0	28.0	35.0	2.0	35.0
94-95	27.799068396017674	34.0	28.0	35.0	2.0	35.0
96-97	27.695400142117656	34.0	27.0	35.0	2.0	35.0
98-99	27.633653814544047	34.0	27.0	35.0	2.0	35.0
100-101	27.570081957885634	34.0	27.0	35.0	2.0	35.0
102-103	26.609741381272332	32.5	25.0	34.5	2.0	35.0
104-105	27.411934848412052	34.0	27.0	35.0	2.0	35.0
106-107	27.735609323120418	34.0	28.5	35.0	2.0	35.0
108-109	27.777393430725233	34.0	29.0	35.0	2.0	35.0
110-111	27.7144914489173	34.0	29.0	35.0	2.0	35.0
112-113	27.640679534804917	34.0	28.0	35.0	2.0	35.0
114-115	27.51825503796969	34.0	27.0	35.0	2.0	35.0
116-117	27.454054272759684	34.0	27.0	35.0	2.0	35.0
118-119	27.296716121250128	34.0	27.0	35.0	2.0	35.0
120-121	27.117893421874484	34.0	27.0	35.0	2.0	35.0
122-123	26.994956846303047	34.0	27.0	35.0	2.0	35.0
124-125	26.15635571897119	33.0	23.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	105471.0
3	17603.0
4	11358.0
5	9528.0
6	9886.0
7	11166.0
8	12345.0
9	12636.0
10	13126.0
11	13679.0
12	13446.0
13	13701.0
14	14846.0
15	12830.0
16	9578.0
17	8439.0
18	7771.0
19	7219.0
20	6958.0
21	7332.0
22	8789.0
23	11673.0
24	16762.0
25	22551.0
26	26857.0
27	28669.0
28	33127.0
29	39041.0
30	44279.0
31	52724.0
32	65189.0
33	83852.0
34	115438.0
35	177340.0
36	327612.0
37	472120.0
38	132195.0
39	99.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.81817248153494	17.88921318788953	12.531955593803612	24.760658736771916
2	16.460997159428647	19.598222303164594	38.81029890500148	25.130481632405278
3	18.663992189041387	24.884024464893688	28.95199599334247	27.499987352722457
4	12.968868179874987	15.488352444481546	36.4700912427685	35.072688132874966
5	13.993683963419201	37.62225810956026	33.79605738466302	14.588000542357516
6	32.88234975633427	35.5071895431451	17.45082789675313	14.159632803767497
7	29.33931653294867	31.470844045024776	21.17234013164186	18.017499290384702
8	27.4860535313683	33.72965960801139	19.64435872553613	19.139928135084187
9	27.66757225755207	14.510399117681283	19.352021896074593	38.470006728692056
10-11	25.99498752700206	25.158369506840355	27.314638551214678	21.532004414942907
12-13	26.238933855477008	23.02853449398991	27.327492103968314	23.40503954656477
14-15	23.95798470897186	24.199950569650625	24.487147476737505	27.35491724464001
16-17	23.829205806138404	27.082528534467908	25.11706107819394	23.971204581199746
18-19	24.010204608317316	26.213688526166322	26.619018847606934	23.157088017909427
20-21	25.10537403131425	25.27435710896726	26.25738415309189	23.362884706626602
22-23	24.86459419755204	24.88964357518887	26.01132434288398	24.234437884375108
24-25	24.37964453653453	25.470697823663112	26.09185041524048	24.05780722456187
26-27	23.98788590281397	25.40509358823202	26.264769432398378	24.34225107655563
28-29	24.069645467660557	25.245507939696658	26.52080321463975	24.164043378003036
30-31	24.449858264624652	25.312161917359322	26.100713585242115	24.137266232773914
32-33	24.085384136764816	25.458184054984873	26.084615425489787	24.371816382760525
34-35	24.33806897455494	25.443262747764805	26.24866834598642	23.969999931693835
36-37	24.54765625316271	25.121144527344054	25.96999117476793	24.361208044725306
38-39	24.378128714191618	25.527068857712347	25.950662250566374	24.14414017752966
40-41	24.363938500898684	25.32243308450864	26.26686043687355	24.046767977719124
42-43	24.30046351519519	25.341386355274604	26.044933493995657	24.313216635534545
44-45	24.290523828019733	25.380563846086694	25.9729526956843	24.355959630209277
46-47	24.368915799177625	25.518867554497003	25.852038005623356	24.260178640702012
48-49	24.457281593191812	25.671905147684278	25.55372432774064	24.317088931383267
50-51	24.372207481160974	25.720282944036903	25.509722058112477	24.397787516689647
52-53	24.57239534016313	25.585731210673963	25.669893301902025	24.171980147260886
54-55	24.4862977307435	25.582494451590442	25.70606231423075	24.225145503435307
56-57	24.406278549469977	25.679071004200054	25.4851464958613	24.42950395046867
58-59	24.472652622960858	25.39619047908194	25.63457016514536	24.496586732811842
60-61	24.47830749292887	25.747859815479046	25.658561610828308	24.115271080763776
62-63	24.269332666782706	25.888306893941543	25.687582728512847	24.154777710762904
64-65	24.398753055504816	25.405127935270606	25.81598612797018	24.380132881254394
66-67	24.30726754033018	25.69727339168157	25.59641157591085	24.399047492077404
68-69	24.42252113807256	25.646018706482764	25.57369709157829	24.357763063866386
70-71	24.279968454857276	25.391512211092365	25.94911840333203	24.379400930718333
72-73	24.508542078997536	25.66519340594404	25.54753997838678	24.278724536671646
74-75	24.25744327637232	25.89343856270628	25.496399732642644	24.35271842827876
76-77	24.262984227078267	25.57026073687917	25.770230178614074	24.396524857428492
78-79	24.329572378356694	25.614218200822954	25.594360237121794	24.461849183698558
80-81	24.265963347934093	25.83181208702178	25.43552499881749	24.466699566226637
82-83	24.557416646116685	25.64604713217115	25.493054106518453	24.30348211519371
84-85	24.160201816632977	25.707010770703203	25.43243169415493	24.700355718508895
86-87	23.94838917238951	25.805540267655164	25.731736804406154	24.51433375554917
88-89	24.25728099103734	25.534499182246478	25.668708900487008	24.539510926229173
90-91	24.15599761049561	25.60252283913992	25.699539549990163	24.541940000374307
92-93	24.350054304415323	25.82803483778737	25.541286278612258	24.280624579185044
94-95	24.18637730665641	25.395761931029387	25.7696779501592	24.648182812155003
96-97	24.367579461175147	25.421986734868074	25.686075405130282	24.5243583988265
98-99	24.13201904412648	25.483924405187185	25.57145657218369	24.812599978502647
100-101	24.121979403532492	25.580004344990197	25.68417788764179	24.61383836383552
102-103	24.264709601387885	25.297277339146536	25.832553575673582	24.605459483792
104-105	24.00871877328205	25.587131337460228	25.721139864034075	24.68301002522365
106-107	23.961538607443675	25.546123702136647	25.756361443010345	24.73597624740933
108-109	24.025970248683013	25.539788599080048	25.581038916171313	24.853202236065624
110-111	24.048392064947617	25.66280124282978	25.677519762520983	24.611286929701617
112-113	24.002346913227434	25.593493385397043	25.914701843389064	24.48945785798646
114-115	23.76017423922082	25.813913847486052	25.849573000608988	24.576338912684136
116-117	23.82596816072539	25.885893206999288	25.64235302805416	24.64578560422116
118-119	23.912659385240218	25.334486883520917	25.808133644180124	24.944720087058734
120-121	23.552152163888636	25.55452096915452	26.01719172983946	24.87613513711738
122-123	23.633880610757153	25.672815316887693	25.91121842834551	24.782085644009644
124-125	23.629627662735547	25.676085419962092	25.91744018288743	24.776846734414928
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	105.0
1	124.5
2	157.5
3	174.0
4	171.0
5	144.5
6	139.5
7	182.0
8	234.0
9	271.5
10	308.0
11	370.5
12	443.0
13	520.5
14	592.0
15	672.0
16	765.0
17	897.0
18	1018.5
19	1084.0
20	1171.0
21	1302.0
22	1564.5
23	1897.5
24	2250.0
25	2988.5
26	4615.5
27	6404.5
28	7334.0
29	9182.0
30	12928.0
31	16131.5
32	20210.5
33	25234.5
34	29877.5
35	34398.0
36	39927.0
37	44932.5
38	45652.0
39	46450.5
40	47603.0
41	48186.5
42	49287.5
43	53051.5
44	57688.5
45	60477.5
46	63267.0
47	65788.0
48	70301.0
49	75313.0
50	76321.0
51	76005.0
52	75950.0
53	76495.0
54	78109.0
55	78346.0
56	75390.5
57	69505.0
58	65427.5
59	60851.0
60	54413.5
61	48618.5
62	41977.5
63	33146.0
64	24725.0
65	19291.5
66	14968.5
67	11252.5
68	8751.5
69	6967.5
70	5841.0
71	4771.5
72	3919.5
73	3617.0
74	2881.0
75	2134.5
76	1481.0
77	848.5
78	514.0
79	336.0
80	192.5
81	91.5
82	61.5
83	44.5
84	28.0
85	21.0
86	19.5
87	15.0
88	13.0
89	11.5
90	9.0
91	12.0
92	13.0
93	8.0
94	6.5
95	8.5
96	9.0
97	9.0
98	9.0
99	10.0
100	28.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03358224996017165
2	0.026299352378447684
3	0.026552230766701986
4	0.03236843369655099
5	0.034340885124934564
6	0.04865380190012821
7	0.041067450252499076
8	0.03788118256049484
9	0.03160979853178808
10-11	0.03871568124173404
12-13	0.03881683259703576
14-15	0.036237473036841855
16-17	0.04094101105837192
18-19	0.05406539940877033
20-21	0.06562194175199206
22-23	0.05755512116667973
24-25	0.0704013432899984
26-27	0.05184006959213245
28-29	0.051890645269783306
30-31	0.05143546417092556
32-33	0.06071610101985854
34-35	0.04245828138789775
36-37	0.05477345889588238
38-39	0.06491388226488
40-41	0.051132010105020394
42-43	0.06311844570827443
44-45	0.0633460362577033
46-47	0.06926339054285403
48-49	0.051890645269783306
50-51	0.054849322412358674
52-53	0.06504032145900715
54-55	0.0502216479073049
56-57	0.048072181607143306
58-59	0.04936186138724026
60-61	0.035984594648587546
62-63	0.04506292878691708
64-65	0.044708899043361054
66-67	0.03886740827468662
68-69	0.032747751278932445
70-71	0.051561903365052716
72-73	0.03373397699312424
74-75	0.04319162871383523
76-77	0.03454318783553801
78-79	0.03530182300030092
80-81	0.025869459118415362
82-83	0.01757504798367417
84-85	0.019775089961486622
86-87	0.018358970987262517
88-89	0.024175173917111523
90-91	0.012947373478620395
92-93	0.021039481902758145
94-95	0.016209504687100924
96-97	0.01251748021858808
98-99	0.01251748021858808
100-101	0.012416328863286356
102-103	0.006321959706357616
104-105	0.006170232673405033
106-107	0.009482939559536423
108-109	0.014186477581066491
110-111	0.006777140805215364
112-113	0.008597865200646357
114-115	0.00925534901010755
116-117	0.0075104881311528465
118-119	0.2414229972663846
120-121	0.35622978553383894
122-123	0.02736144160911576
124-125	0.003160979853178808
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1977235.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.39187348640456
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.88038391642397	41.850829681947104
2	10.069295317653317	10.550984927594769
3	3.4834680166886844	5.475162468728705
4	1.7514817157895517	3.6705363386958796
5	1.0573595875090904	2.769852486920659
6	0.6974199937209319	2.1923484046749677
7	0.4910883419722077	1.8010326798279261
8	0.35802818744853493	1.5006214001096305
9	0.28634393012669185	1.3501885464717756
>10	1.7194591910914832	16.73561669377916
>50	0.13636577208458073	4.923507316219392
>100	0.0668696644945089	6.246433026255085
>500	0.0021440011969857523	0.7122120965125721
>1k	2.9236379958896613E-4	0.22067393226233678
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.0115135530172185E-4
7	0.0	0.0	0.0	0.0	1.0115135530172185E-4
8	0.0	0.0	0.0	0.0	1.0115135530172185E-4
9	0.0	0.0	0.0	0.0	1.0115135530172185E-4
10-11	0.0	0.0	0.0	5.0575677650860924E-5	1.0115135530172185E-4
12-13	0.0	0.0	0.0	5.0575677650860924E-5	1.5172703295258277E-4
14-15	0.0	0.0	0.0	5.0575677650860924E-5	1.5172703295258277E-4
16-17	0.0	0.0	0.0	5.0575677650860924E-5	1.5172703295258277E-4
18-19	0.0	0.0	0.0	5.0575677650860924E-5	1.5172703295258277E-4
20-21	0.0	0.0	0.0	5.0575677650860924E-5	1.5172703295258277E-4
22-23	0.0	0.0	0.0	1.5172703295258277E-4	1.7701487177801325E-4
24-25	0.0	0.0	0.0	2.023027106034437E-4	2.023027106034437E-4
26-27	0.0	0.0	0.0	2.2759054942887415E-4	2.5287838825430463E-4
28-29	0.0	0.0	0.0	8.850743588900661E-4	2.5287838825430463E-4
30-31	0.0	0.0	0.0	0.0032368433696550987	2.5287838825430463E-4
32-33	0.0	0.0	0.0	0.00869901655594808	2.5287838825430463E-4
34-35	0.0	0.0	0.0	0.015956626298846623	3.0345406590516553E-4
36-37	0.0	0.0	0.0	0.02463035501596927	3.0345406590516553E-4
38-39	0.0	0.0	0.0	0.03952489208414781	3.0345406590516553E-4
40-41	0.0	0.0	0.0	0.05765627252198145	3.0345406590516553E-4
42-43	0.0	0.0	0.0	0.0748014272456233	3.0345406590516553E-4
44-45	0.0	0.0	0.0	0.09581562130955602	3.540297435560265E-4
46-47	0.0	0.0	0.0	0.12049655200317616	4.046054212068874E-4
48-49	0.0	0.0	0.0	0.14750396386873588	4.046054212068874E-4
50-51	0.0	0.0	0.0	0.17701487177801323	4.046054212068874E-4
52-53	0.0	0.0	0.0	0.206374052654338	5.310446153340396E-4
54-55	0.0	0.0	0.0	0.23836316876850755	6.069081318103311E-4
56-57	0.0	0.0	0.0	0.2762443513290024	6.069081318103311E-4
58-59	0.0	0.0	0.0	0.31875320839455096	6.069081318103311E-4
60-61	0.0	0.0	0.0	0.3639931520532461	6.57483809461192E-4
62-63	0.0	0.0	0.0	0.4101434579096567	7.08059487112053E-4
64-65	0.0	0.0	0.0	0.45872139629330855	7.08059487112053E-4
66-67	0.0	0.0	0.0	0.5132166889621113	7.08059487112053E-4
68-69	0.0	0.0	0.0	0.580052446977724	7.08059487112053E-4
70-71	0.0	0.0	0.0	0.6518951970807719	7.08059487112053E-4
72-73	0.0	0.0	0.0	0.7259632770004577	8.344986812392053E-4
74-75	0.0	0.0	0.0	0.8044314408757685	8.597865200646357E-4
76-77	0.0	0.0	0.0	0.8921296659223612	8.597865200646357E-4
78-79	0.0	0.0	0.0	0.9932304455464323	8.597865200646357E-4
80-81	0.0	0.0	0.0	1.1038647404076904	8.850743588900661E-4
82-83	0.0	0.0	0.0	1.2239061113120089	9.103621977154966E-4
84-85	0.0	0.0	0.0	1.3460716606776635	9.103621977154966E-4
86-87	0.0	0.0	0.0	1.479161556415904	9.356500365409271E-4
88-89	0.0	0.0	0.0	1.624086160724446	9.609378753663576E-4
90-91	0.0	0.0	0.0	1.7893674752874595	9.609378753663576E-4
92-93	0.0	0.0	0.0	1.9685823890432852	9.609378753663576E-4
94-95	0.0	0.0	0.0	2.156749197743313	9.609378753663576E-4
96-97	0.0	0.0	0.0	2.351111526955572	9.86225714191788E-4
98-99	0.0	0.0	0.0	2.57364450861936	0.0010115135530172185
100-101	0.0	0.0	0.0	2.8189365452260353	0.0010115135530172185
102-103	0.0	5.0575677650860924E-5	0.0	3.09472571545618	0.0010115135530172185
104-105	0.0	5.0575677650860924E-5	0.0	3.3832599564543413	0.001036801391842649
106-107	0.0	5.0575677650860924E-5	0.0	3.676497735474033	0.0010620892306680795
108-109	0.0	5.0575677650860924E-5	0.0	3.984933505627809	0.0010620892306680795
110-111	0.0	5.0575677650860924E-5	0.0	4.322070972848447	0.0011126649083189402
112-113	0.0	5.0575677650860924E-5	0.0	4.69023661830789	0.0011632405859698012
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2340	0.0	71.48122	1
GTATCAA	4480	0.0	55.672085	1
TCAACGC	5535	0.0	43.643845	4
CAACGCA	5700	0.0	42.27501	5
AACGCAG	5890	0.0	40.60414	6
ATCAACG	6220	0.0	38.4538	3
TATCAAC	6575	0.0	37.191067	2
GTACATG	10440	0.0	36.83269	1
TACATGG	11050	0.0	34.513447	2
ACATGGG	10960	0.0	34.20059	3
ACGCAGA	7110	0.0	33.384197	7
CATGGGG	8280	0.0	31.258884	4
CGCAGAG	7540	0.0	31.163155	8
GCAGAGT	7865	0.0	28.439093	9
CAGAGTA	7630	0.0	25.650951	10-11
AGAGTAC	7285	0.0	24.701761	10-11
ATGGGGG	4440	0.0	23.718864	5
CATGGGT	2145	0.0	22.745794	4
ATGGGGA	3480	0.0	22.56827	5
GTATTAC	450	0.0	22.487328	1
>>END_MODULE