FastQCFastQC Report
Mon 15 Aug 2016
SRR2921510_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921510_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1977235
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26100.13200251866874702No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23670.11971262899958782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27500.076.762111
GTATCAA56750.058.4231871
TCAACGC73850.043.702774
CAACGCA76150.042.2799725
AACGCAG78850.040.747466
ATCAACG81100.040.017213
TATCAAC86400.037.975022
GTACATG104450.036.7095341
ACATGGG109150.034.534343
TACATGG110800.034.180432
ACGCAGA94550.033.915857
CATGGGG80950.032.7343184
CGCAGAG99250.032.247378
GCAGAGT107300.029.439979
AGAGTAC98300.027.29424510-11
ATGGGGG42850.025.9715655
CAGAGTA102650.025.5290710-11
TACCGTA2451.6239937E-824.2833987
GAGTACT52950.023.03251812-13
AGTACTT58000.022.05281412-13
Produced by FastQC (version 0.11.5)