##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921510_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1977235
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.291069093962022	31.0	31.0	34.0	28.0	34.0
2	31.344609517836776	33.0	31.0	34.0	28.0	34.0
3	31.42878312390788	33.0	31.0	34.0	28.0	34.0
4	34.96010894000966	37.0	35.0	37.0	32.0	37.0
5	34.63700672909391	37.0	35.0	37.0	31.0	37.0
6	34.618039838461286	37.0	35.0	37.0	31.0	37.0
7	34.45844019552557	37.0	35.0	37.0	30.0	37.0
8	34.4242722792182	37.0	35.0	37.0	30.0	37.0
9	35.99914678831803	39.0	37.0	39.0	30.0	39.0
10-11	35.86819472647409	39.0	36.5	39.0	30.0	39.0
12-13	35.796764168143895	39.0	36.0	39.0	30.0	39.0
14-15	36.97682976479781	40.0	37.0	41.0	30.0	41.0
16-17	36.9522090191606	40.0	37.0	41.0	30.0	41.0
18-19	36.97588450538252	40.0	37.0	41.0	30.0	41.0
20-21	36.95973948468442	40.0	37.0	41.0	30.0	41.0
22-23	36.897138933915286	40.0	37.0	41.0	30.0	41.0
24-25	36.84023371020642	40.0	37.0	41.0	30.0	41.0
26-27	36.72252387804181	40.0	36.5	41.0	30.0	41.0
28-29	36.52961762258912	40.0	36.0	41.0	29.5	41.0
30-31	36.4911578542763	40.0	36.0	41.0	30.0	41.0
32-33	36.378984035787354	40.0	36.0	41.0	29.5	41.0
34-35	36.26900874200588	40.0	35.0	41.0	29.0	41.0
36-37	36.13726415929315	40.0	35.0	41.0	28.5	41.0
38-39	35.97015630413178	40.0	35.0	41.0	28.0	41.0
40-41	35.774618849049304	40.0	35.0	41.0	27.0	41.0
42-43	35.610836344693475	39.0	35.0	41.0	27.0	41.0
44-45	35.41013941185544	39.0	35.0	41.0	26.0	41.0
46-47	35.15061158638199	39.0	35.0	41.0	24.5	41.0
48-49	34.908750350868765	39.0	34.5	41.0	24.0	41.0
50-51	34.69738296155995	39.0	34.0	41.0	23.5	41.0
52-53	34.51097011736086	38.5	34.0	41.0	23.0	41.0
54-55	34.294320857156585	38.0	34.0	40.0	23.0	41.0
56-57	34.041396445035616	38.0	33.5	40.0	22.0	41.0
58-59	33.89713185332042	37.0	33.5	40.0	22.0	41.0
60-61	33.78409951270335	37.0	33.0	40.0	22.0	41.0
62-63	33.511191891707355	36.5	33.0	40.0	22.0	41.0
64-65	33.22202368458984	36.0	33.0	39.5	21.5	41.0
66-67	32.91934443806629	35.5	33.0	39.0	20.5	41.0
68-69	32.59886685194223	35.0	33.0	39.0	20.0	41.0
70-71	32.29588187544728	35.0	32.0	37.5	20.0	40.0
72-73	31.981472106249385	35.0	32.0	37.0	19.5	39.5
74-75	31.68281792503167	35.0	32.0	37.0	18.0	39.0
76-77	31.39319908862629	35.0	32.0	36.0	18.0	39.0
78-79	31.114128568430154	35.0	32.0	36.0	17.0	37.5
80-81	30.887412472467865	35.0	32.0	35.0	15.5	37.0
82-83	30.694080622687743	35.0	31.0	35.0	14.5	37.0
84-85	30.493651740941264	35.0	31.0	35.0	12.5	36.0
86-87	30.353145933589076	35.0	31.0	35.0	11.0	36.0
88-89	30.24411488770935	35.0	31.0	35.0	10.0	36.0
90-91	30.142067837156432	35.0	31.0	35.0	8.0	35.5
92-93	30.201410049892907	35.0	31.0	35.0	8.5	35.0
94-95	30.1674970350009	35.0	31.0	35.0	7.0	35.0
96-97	30.140333597169786	35.0	31.0	35.0	7.0	35.0
98-99	30.101265150576438	35.0	31.0	35.0	7.0	35.0
100-101	30.05361451724251	35.0	31.0	35.0	6.0	35.0
102-103	29.164720177419476	33.5	29.5	35.0	2.0	35.0
104-105	29.872564717901515	34.5	31.0	35.0	2.0	35.0
106-107	29.98281994805878	35.0	31.0	35.0	2.0	35.0
108-109	29.99138873224478	35.0	32.0	35.0	2.0	35.0
110-111	29.95671303613379	35.0	32.0	35.0	2.0	35.0
112-113	29.909318062850396	35.0	31.5	35.0	2.0	35.0
114-115	29.847161313652652	35.0	31.0	35.0	2.0	35.0
116-117	29.77031258297572	35.0	31.0	35.0	2.0	35.0
118-119	29.678509635930986	35.0	31.0	35.0	2.0	35.0
120-121	29.560609639218406	35.0	31.0	35.0	2.0	35.0
122-123	29.442730631412047	35.0	31.0	35.0	2.0	35.0
124-125	28.53060131951943	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	29226.0
3	11037.0
4	6245.0
5	5335.0
6	5761.0
7	6648.0
8	7706.0
9	8773.0
10	10323.0
11	10851.0
12	11887.0
13	13443.0
14	16703.0
15	13559.0
16	7742.0
17	6671.0
18	5976.0
19	5578.0
20	5290.0
21	5706.0
22	6942.0
23	9374.0
24	12981.0
25	17841.0
26	22520.0
27	25705.0
28	29891.0
29	35981.0
30	41929.0
31	50064.0
32	62184.0
33	80746.0
34	110681.0
35	173290.0
36	344048.0
37	568527.0
38	189786.0
39	285.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.062155186426565	18.50350339452268	12.671314903440805	24.763026515609944
2	16.804884080965042	19.982662342030316	37.953589901674036	25.258863675330606
3	18.666153385430366	25.05610008781763	29.313535246435695	26.96421128031631
4	13.159609944689873	15.992672546846615	36.053498504653035	34.794219003810475
5	14.02283278503419	37.60423206333323	33.73231096579736	14.640624185835216
6	32.90259917643845	35.950232195793156	16.827922176468803	14.31924645129959
7	29.204484810776492	31.70481993226752	20.99919317920274	18.091502077753244
8	27.662888966779608	33.78398678083221	19.316089137329936	19.237035115058248
9	27.74798168265102	14.255829879695034	19.254256902134497	38.741931535519456
10-11	26.103323500611005	25.056472399588888	27.181989325629015	21.658214774171096
12-13	26.438327445594794	22.95704661020271	27.407281710902893	23.197344233299606
14-15	24.120850691924353	23.97865228379798	24.793960247161724	27.10653677711594
16-17	23.832315672150816	26.890332096527036	25.353483724460364	23.923868506861783
18-19	23.902011819235693	25.734200724691924	27.334334454832188	23.029453001240192
20-21	24.913805923718243	24.802370741764772	27.129206134347427	23.154617200169554
22-23	24.73268117994578	24.434103508274994	26.92864079634201	23.90457451543722
24-25	24.185445625262876	25.052701810311927	27.01167899937504	23.750173565050154
26-27	23.79422921436518	25.033073357731705	27.171998404689425	24.00069902321369
28-29	23.969882291050173	24.864257856958588	27.319073705935022	23.84678614605622
30-31	24.264993971224264	24.93791005982235	26.955343048342257	23.84175292061113
32-33	23.85513409449072	25.100742534379428	26.966803080813307	24.07732029031655
34-35	24.074186959738462	25.100435687139957	27.078619712787955	23.746757640333627
36-37	24.397222027614315	24.787730546087214	26.71336244780047	24.101684978498003
38-39	24.245866493454987	25.18221625963945	26.681579868593847	23.890337378311713
40-41	24.267094922262757	24.97627101497841	26.91169125131986	23.844942811438976
42-43	24.209234912174303	25.05724463984308	26.64964965319384	24.08387079478878
44-45	24.239870440379786	25.087304275158896	26.507012037582	24.165813246879317
46-47	24.37377236924705	25.22835259856835	26.20271772093054	24.195157311254064
48-49	24.48503394322973	25.463353731225872	25.79316167671638	24.258450648828017
50-51	24.386082366929397	25.564862900939282	25.619722253346005	24.429332478785316
52-53	24.553844581723684	25.502826736518713	25.74850784077598	24.19482084098162
54-55	24.481609800687057	25.507741897299784	25.814266296581263	24.1963820054319
56-57	24.4245381331067	25.54548857726785	25.640087820473905	24.38988546915154
58-59	24.341855498947957	25.297510274477585	25.896185600985806	24.464448625588652
60-61	24.487317142882436	25.610784190977004	25.85360301398864	24.048295652151918
62-63	24.363557169445077	25.666340707299785	25.936787490849785	24.033314632405354
64-65	24.388290197088157	25.208810047669118	26.03835938521587	24.36454037002686
66-67	24.383365343420095	25.49989099541169	25.762336613250202	24.354407047918013
68-69	24.45877585338591	25.46401210705016	25.730289302951714	24.346922736612218
70-71	24.320356881716158	25.245339739992108	26.117158593890487	24.317144784401247
72-73	24.66017649424459	25.524386203198546	25.626519158149264	24.1889181444076
74-75	24.356384406171426	25.76318891877133	25.55904797628313	24.32137869877412
76-77	24.37437181735547	25.39443673609576	25.80353001340966	24.42766143313911
78-79	24.355530484033558	25.5339875820077	25.697123928224052	24.41335800573469
80-81	24.315360673630106	25.725602977611008	25.456575873813236	24.50246047494565
82-83	24.611441617040196	25.597749541671188	25.472561095123243	24.318247746165376
84-85	24.228628890809357	25.63549264384718	25.433641358243413	24.70223710710005
86-87	24.005040051311	25.742451557033917	25.710002812393146	24.542505579261938
88-89	24.330579423811272	25.420744139858776	25.652410465480095	24.596265970849856
90-91	24.21029283746264	25.486970698044026	25.759307059753656	24.543429404739683
92-93	24.301128568267316	25.677033477697343	25.615578667846865	24.406259286188476
94-95	24.179457330719014	25.275301388096118	25.756394404723576	24.788846876461292
96-97	24.346994425659314	25.34672409175775	25.68144247539873	24.624839007184203
98-99	24.169459559177753	25.404140165482204	25.58270152385341	24.84369875148664
100-101	24.08901824391448	25.43568802643213	25.73692910629705	24.738364623356336
102-103	24.3505097700446	25.149405765626152	25.853772520870784	24.646311943458464
104-105	24.099669402301714	25.47094303417019	25.677753098926676	24.751634464601416
106-107	24.099435017961987	25.414304145544957	25.660633481874402	24.825627354618657
108-109	24.151332150278932	25.373936920402834	25.5049058986121	24.96982503070613
110-111	24.23250136492249	25.43669301091407	25.531219741267847	24.79958588289559
112-113	24.135033091362203	25.35082357267341	25.763235849393013	24.750907486571375
114-115	23.847138738358726	25.581478859900606	25.74964779356037	24.821734608180293
116-117	23.983968166978702	25.71013671012078	25.48159103398899	24.824304088911525
118-119	23.942574119203773	25.174024718034936	25.6802416568792	25.203159505882088
120-121	23.638850695460395	25.408423819515374	25.847795063939465	25.104930421084763
122-123	23.763555009783936	25.48933578997998	25.738144597897755	25.008964602338335
124-125	23.703061982023456	25.52861589205473	25.65017952515312	25.11814260076869
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	34.0
1	32.0
2	60.5
3	84.0
4	59.0
5	53.0
6	90.5
7	225.0
8	399.0
9	461.0
10	392.0
11	333.5
12	353.0
13	392.5
14	486.5
15	636.5
16	791.5
17	920.5
18	1079.0
19	1280.0
20	1525.5
21	1774.5
22	2015.0
23	2328.5
24	2721.5
25	3503.5
26	5097.5
27	6836.5
28	7764.5
29	9608.0
30	13322.5
31	16480.0
32	20462.5
33	25507.0
34	30269.5
35	34551.0
36	39736.0
37	44664.5
38	45295.0
39	46067.0
40	47265.0
41	47369.5
42	48028.0
43	52151.0
44	57154.0
45	59956.0
46	62941.5
47	65682.5
48	70551.5
49	75942.0
50	76939.5
51	76490.5
52	76235.5
53	76881.5
54	78710.5
55	78682.0
56	75504.0
57	69668.0
58	65885.0
59	61614.0
60	54797.5
61	48641.5
62	41849.0
63	32692.0
64	24161.5
65	18795.0
66	14379.5
67	10485.5
68	8121.0
69	6453.0
70	5456.5
71	4616.5
72	3802.5
73	3568.5
74	2900.0
75	2100.0
76	1378.5
77	732.0
78	421.5
79	279.5
80	150.5
81	53.5
82	34.0
83	14.0
84	7.0
85	7.5
86	4.5
87	1.5
88	0.0
89	0.5
90	0.5
91	1.5
92	1.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12320235075749722
2	0.0018207243954309932
3	0.02078660351450384
4	0.05588612380420132
5	0.026097049667844235
6	0.02301193333114172
7	0.017195730401292714
8	0.005361021830991258
9	0.004703538021530066
10-11	0.00824383545709033
12-13	0.0052345826368641055
14-15	0.003439146080258543
16-17	0.011859996409126886
18-19	0.00601850564045245
20-21	0.01522327897290914
22-23	0.00986225714191788
24-25	0.015703747910592317
26-27	0.009634666592489006
28-29	0.0164370952365298
30-31	0.02354297794647576
32-33	0.014641658679924239
34-35	0.023897007690031787
36-37	0.016462383075355233
38-39	0.023416538752348608
40-41	0.011253088277316557
42-43	0.00981168146426702
44-45	0.019446348056756026
46-47	0.018106092599008212
48-49	0.024200461755936955
50-51	0.018283107470786222
52-53	0.02455449149949298
54-55	0.019926816994439205
56-57	0.024023446884158937
58-59	0.018738288569643973
60-61	0.02288549413701457
62-63	0.02559129289133563
64-65	0.01914289399085086
66-67	0.012846222123318676
68-69	0.027083275382036025
70-71	0.01686698849656212
72-73	0.02023027106034437
74-75	0.020963618386281854
76-77	0.01527385465056
78-79	0.033759264831949666
80-81	0.024402764466540397
82-83	0.010899058533760528
84-85	0.014388780291669933
86-87	0.013706008643383309
88-89	0.023745280657079204
90-91	0.013554281610430728
92-93	0.00872430439477351
94-95	0.00882545575007523
96-97	0.002554071721368477
98-99	0.00220004197781245
100-101	0.012492192379762648
102-103	0.003489721757909404
104-105	0.002958677142575364
106-107	2.0230271060344367E-4
108-109	0.021798117067521057
110-111	8.344986812392053E-4
112-113	0.010545028790204504
114-115	0.002857525787273642
116-117	0.008522001684170066
118-119	0.01097492205023682
120-121	0.004627674505053775
122-123	7.08059487112053E-4
124-125	0.024908521243049004
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1977235.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.136918056640354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.64737893638073	38.153528961098424
2	11.214902881320853	11.021315277852851
3	3.882464721803676	5.723170526791927
4	1.8939664300246604	3.7225469309659767
5	1.1509585140280012	2.8277277095193223
6	0.7509639985946305	2.2140033877458807
7	0.544250475283292	1.8719953704397878
8	0.40662060995871774	1.5984066873346108
9	0.3258936065833532	1.4412066697682298
>10	1.941740033988758	17.881366496930763
>50	0.16029629643099	5.443147557851491
>100	0.07735809360920859	6.844807509809482
>500	0.002791801735957146	0.8673874302212154
>1k	4.136002571788364E-4	0.3893894836700677
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2610	0.13200251866874702	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2367	0.11971262899958782	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.0575677650860924E-5
7	0.0	0.0	0.0	0.0	5.0575677650860924E-5
8	0.0	0.0	0.0	0.0	5.0575677650860924E-5
9	0.0	0.0	0.0	0.0	5.0575677650860924E-5
10-11	0.0	0.0	0.0	0.0	5.0575677650860924E-5
12-13	0.0	0.0	0.0	0.0	5.0575677650860924E-5
14-15	0.0	0.0	0.0	0.0	5.0575677650860924E-5
16-17	0.0	0.0	0.0	0.0	5.0575677650860924E-5
18-19	0.0	0.0	0.0	0.0	1.0115135530172185E-4
20-21	0.0	0.0	0.0	5.0575677650860924E-5	1.0115135530172185E-4
22-23	0.0	0.0	0.0	1.2643919412715231E-4	1.5172703295258277E-4
24-25	0.0	0.0	0.0	1.5172703295258277E-4	2.5287838825430463E-4
26-27	0.0	0.0	0.0	2.023027106034437E-4	3.7931758238145697E-4
28-29	0.0	0.0	0.0	0.0010115135530172185	4.551810988577483E-4
30-31	0.0	0.0	0.0	0.0034138582414331123	4.551810988577483E-4
32-33	0.0	0.0	0.0	0.009053046299504106	4.551810988577483E-4
34-35	0.0	0.0	0.0	0.016588822269482384	4.551810988577483E-4
36-37	0.0	0.0	0.0	0.025818883440764502	4.551810988577483E-4
38-39	0.0	0.0	0.0	0.04162378270665854	4.551810988577483E-4
40-41	0.0	0.0	0.0	0.06010919288804821	4.551810988577483E-4
42-43	0.0	0.0	0.0	0.07773481654937324	4.551810988577483E-4
44-45	0.0	0.0	0.0	0.09933063090629085	4.551810988577483E-4
46-47	0.0	0.0	0.0	0.12487134811997562	4.551810988577483E-4
48-49	0.0	0.0	0.0	0.152915561377378	5.563324541594702E-4
50-51	0.0	0.0	0.0	0.1829575139019894	5.563324541594702E-4
52-53	0.0	0.0	0.0	0.21274658803834648	6.069081318103311E-4
54-55	0.0	0.0	0.0	0.2452414609290246	6.57483809461192E-4
56-57	0.0	0.0	0.0	0.28426059623666383	6.57483809461192E-4
58-59	0.0	0.0	0.0	0.3286913290529452	6.57483809461192E-4
60-61	0.0	0.0	0.0	0.37547383087999153	6.57483809461192E-4
62-63	0.0	0.0	0.0	0.4228632408388482	6.57483809461192E-4
64-65	0.0	0.0	0.0	0.4735906455226617	6.57483809461192E-4
66-67	0.0	0.0	0.0	0.5297296477151173	6.57483809461192E-4
68-69	0.0	0.0	0.0	0.5979056611884779	6.57483809461192E-4
70-71	0.0	0.0	0.0	0.6717461505587348	6.827716482866225E-4
72-73	0.0	0.0	0.0	0.7480395602950585	7.08059487112053E-4
74-75	0.0	0.0	0.0	0.8285054634375782	7.08059487112053E-4
76-77	0.0	0.0	0.0	0.9188841993996667	7.586351647629138E-4
78-79	0.0	0.0	0.0	1.023879306202854	7.586351647629138E-4
80-81	0.0	0.0	0.0	1.1379527471443707	7.586351647629138E-4
82-83	0.0	0.0	0.0	1.2608010681583122	7.586351647629138E-4
84-85	0.0	0.0	0.0	1.3862034608936216	7.586351647629138E-4
86-87	0.0	0.0	0.0	1.5234152743604072	8.092108424137748E-4
88-89	0.0	0.0	0.0	1.673195143723432	8.597865200646357E-4
90-91	0.0	0.0	0.0	1.844140934183342	8.597865200646357E-4
92-93	0.0	0.0	0.0	2.031093926619749	9.609378753663576E-4
94-95	0.0	0.0	0.0	2.22616431531912	9.609378753663576E-4
96-97	0.0	0.0	0.0	2.426848604237736	0.001036801391842649
98-99	0.0	0.0	0.0	2.6562345902232156	0.0010620892306680795
100-101	0.0	0.0	0.0	2.908733660895139	0.0010620892306680795
102-103	0.0	0.0	0.0	3.1914011232858006	0.0010620892306680795
104-105	0.0	0.0	0.0	3.4876481551257186	0.0010620892306680795
106-107	0.0	0.0	0.0	3.789104481763675	0.0010620892306680795
108-109	0.0	0.0	0.0	4.106466859022827	0.0010620892306680795
110-111	0.0	0.0	0.0	4.4555401861690695	0.001137952747144371
112-113	0.0	0.0	0.0	4.83715896188364	0.0011632405859698012
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2750	0.0	76.76211	1
GTATCAA	5675	0.0	58.423187	1
TCAACGC	7385	0.0	43.70277	4
CAACGCA	7615	0.0	42.279972	5
AACGCAG	7885	0.0	40.74746	6
ATCAACG	8110	0.0	40.01721	3
TATCAAC	8640	0.0	37.97502	2
GTACATG	10445	0.0	36.709534	1
ACATGGG	10915	0.0	34.53434	3
TACATGG	11080	0.0	34.18043	2
ACGCAGA	9455	0.0	33.91585	7
CATGGGG	8095	0.0	32.734318	4
CGCAGAG	9925	0.0	32.24737	8
GCAGAGT	10730	0.0	29.43997	9
AGAGTAC	9830	0.0	27.294245	10-11
ATGGGGG	4285	0.0	25.971565	5
CAGAGTA	10265	0.0	25.52907	10-11
TACCGTA	245	1.6239937E-8	24.283398	7
GAGTACT	5295	0.0	23.032518	12-13
AGTACTT	5800	0.0	22.052814	12-13
>>END_MODULE