FastQCFastQC Report
Mon 15 Aug 2016
SRR2921509_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921509_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1551266
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16600.072.058091
GTATCAA32050.055.3328481
TCAACGC40450.042.944254
CAACGCA42000.041.6426855
AACGCAG44200.039.3033266
ATCAACG44900.038.8205763
GTACATG80850.037.760011
TATCAAC47150.037.0942272
TACATGG83050.036.245292
ACATGGG84850.035.6166083
CATGGGG69050.033.169414
ACGCAGA52400.033.0392657
CGCAGAG56700.030.3208568
ATGGGGG38500.028.585895
ATGGGTA9400.028.4790425
GCAGAGT61250.027.1952239
GGTACCT8250.026.6793028
TACCGTA1401.0569507E-425.4971667
CATGGGT14850.025.2379934
AGAGTAC53350.025.14506510-11
Produced by FastQC (version 0.11.5)