##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921509_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1551266
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.85255849093579	31.0	30.0	34.0	20.0	34.0
2	28.861848967230635	31.0	30.0	34.0	16.0	34.0
3	28.965316070873726	31.0	30.0	34.0	20.0	34.0
4	32.32565208030086	35.0	33.0	37.0	20.0	37.0
5	32.32796889766165	35.0	33.0	37.0	22.0	37.0
6	32.3900807469512	37.0	33.0	37.0	21.0	37.0
7	32.17617932707866	37.0	33.0	37.0	18.0	37.0
8	32.12039972512773	36.0	33.0	37.0	18.0	37.0
9	33.4939294743777	39.0	34.0	39.0	17.0	39.0
10-11	33.35275155904919	38.0	33.0	39.0	17.0	39.0
12-13	33.18975469068489	38.0	33.0	39.0	16.5	39.0
14-15	34.21773087271944	39.0	33.0	41.0	17.0	41.0
16-17	34.11920940702626	39.0	33.0	41.0	16.0	41.0
18-19	34.12237746459988	39.0	33.5	41.0	16.0	41.0
20-21	34.1548425608503	39.0	34.0	41.0	16.0	41.0
22-23	34.1016685726368	39.0	33.0	41.0	16.0	41.0
24-25	34.0509245351861	39.0	33.0	41.0	16.0	41.0
26-27	33.96774602163652	39.0	33.0	41.0	15.0	41.0
28-29	33.910243310947315	39.0	33.0	41.0	15.0	41.0
30-31	33.80651996498344	39.0	33.0	41.0	11.0	41.0
32-33	33.69505133226668	39.0	33.0	41.0	9.5	41.0
34-35	33.52143056058729	39.0	33.0	41.0	9.0	41.0
36-37	33.36013778423559	38.5	33.0	41.0	8.0	41.0
38-39	33.22352646161264	38.0	32.0	41.0	7.5	41.0
40-41	33.064908919553446	38.0	32.0	41.0	4.0	41.0
42-43	32.855703341657716	38.0	32.0	40.5	2.0	41.0
44-45	32.64459125643184	38.0	31.5	40.0	2.0	41.0
46-47	32.38901677726451	38.0	31.0	40.0	2.0	41.0
48-49	32.24348596565643	38.0	31.0	40.0	2.0	41.0
50-51	32.04511089651936	37.5	31.0	40.0	2.0	41.0
52-53	31.84521642323109	37.0	31.0	40.0	2.0	41.0
54-55	31.63565597389487	37.0	30.5	40.0	2.0	41.0
56-57	31.395170783089426	36.5	30.0	40.0	2.0	41.0
58-59	31.147068265532795	36.0	30.0	40.0	2.0	41.0
60-61	30.846290384756706	35.5	30.0	39.0	2.0	41.0
62-63	30.538648432957338	35.0	29.0	39.0	2.0	41.0
64-65	30.237410605273368	35.0	29.0	39.0	2.0	40.0
66-67	30.212199584081645	35.0	29.0	38.5	2.0	40.0
68-69	29.982040797645276	35.0	29.0	37.5	2.0	40.0
70-71	29.685040798934548	35.0	29.0	37.0	2.0	39.5
72-73	29.375118451638855	35.0	29.0	36.5	2.0	39.0
74-75	29.088762017603685	35.0	29.0	36.0	2.0	39.0
76-77	28.822395707763853	34.5	29.0	36.0	2.0	38.5
78-79	28.551760626481855	34.0	29.0	35.0	2.0	37.0
80-81	28.320042790855986	34.0	28.0	35.0	2.0	37.0
82-83	28.125758896282132	34.0	28.0	35.0	2.0	36.0
84-85	27.926949020993177	34.0	28.0	35.0	2.0	36.0
86-87	27.77749915230528	34.0	27.0	35.0	2.0	36.0
88-89	27.648379774970895	34.0	27.0	35.0	2.0	35.0
90-91	27.537338212788782	34.0	27.0	35.0	2.0	35.0
92-93	27.43326225160611	34.0	27.0	35.0	2.0	35.0
94-95	27.35961434080293	34.0	27.0	35.0	2.0	35.0
96-97	27.2598706475872	34.0	27.0	35.0	2.0	35.0
98-99	27.196471140346013	34.0	26.5	35.0	2.0	35.0
100-101	27.13118962189592	34.0	26.0	35.0	2.0	35.0
102-103	26.15987038973329	32.5	24.5	34.0	2.0	35.0
104-105	26.973408171132483	33.5	26.0	35.0	2.0	35.0
106-107	27.300134857593733	34.0	27.0	35.0	2.0	35.0
108-109	27.34042904311704	34.0	27.0	35.0	2.0	35.0
110-111	27.27968027404713	34.0	27.0	35.0	2.0	35.0
112-113	27.204484272845534	34.0	27.0	35.0	2.0	35.0
114-115	27.080768224147246	34.0	26.5	35.0	2.0	35.0
116-117	27.008458252807706	34.0	27.0	35.0	2.0	35.0
118-119	26.846301021230403	34.0	25.5	35.0	2.0	35.0
120-121	26.663384293860627	34.0	25.0	35.0	2.0	35.0
122-123	26.532094753575464	34.0	25.0	35.0	2.0	35.0
124-125	25.679326111704892	33.0	22.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	91339.0
3	14950.0
4	9411.0
5	7981.0
6	8229.0
7	9208.0
8	10056.0
9	10472.0
10	10700.0
11	11202.0
12	11036.0
13	11261.0
14	11873.0
15	10255.0
16	7794.0
17	6899.0
18	6393.0
19	5915.0
20	5819.0
21	5950.0
22	7450.0
23	9797.0
24	13582.0
25	18504.0
26	21949.0
27	23505.0
28	27459.0
29	32159.0
30	36705.0
31	43267.0
32	53518.0
33	68562.0
34	93686.0
35	144985.0
36	259571.0
37	343680.0
38	86068.0
39	76.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.795350192946614	17.159544666625397	12.047620153119132	24.99748498730886
2	16.86769517260768	19.614303824076735	37.86929557702897	25.64870542628661
3	19.040053235803143	24.51084447356136	28.89950968380925	27.549592606826256
4	13.331299946671962	15.239824630073015	35.61519726537437	35.81367815788065
5	14.271564304387471	37.2655787517395	33.38045080587644	15.082406137996587
6	33.304139937404024	34.79242270145539	17.431810955778452	14.471626405362127
7	29.645350828146732	31.219613176829302	20.82223301352804	18.31280298149592
8	28.206854435934492	33.1286559323881	19.487816406821807	19.1766732248556
9	27.925973690998195	14.369744647923651	18.82583182873356	38.8784498323446
10-11	26.439981079799203	24.852399541246392	26.967831467053927	21.73978791190048
12-13	26.578366488621835	22.635069995930955	26.988753060649383	23.797810454797826
14-15	24.137217254204007	23.75285546855478	24.314743971072108	27.7951833061691
16-17	24.34759253455496	26.67109687254549	24.77626493018073	24.20504566271882
18-19	24.384364648580597	25.876968382057026	26.272547390797364	23.466119578565017
20-21	25.58115234239572	24.85857193859566	25.871539039821094	23.688736679187524
22-23	25.30020549031951	24.44431831521157	25.751497318822164	24.503978875646755
24-25	24.740693676666947	24.983777228776553	25.772242612672468	24.503286481884036
26-27	24.38752335090906	24.95839321882829	25.968113452929114	24.685969977333535
28-29	24.444420079850655	25.027901938672812	25.98822523676742	24.539452744709113
30-31	24.856208602330845	24.94979071402313	25.80947559190202	24.38452509174401
32-33	24.49671052631579	25.06591847265222	25.7218459752322	24.715525025799796
34-35	24.736944777185382	25.113200885422295	25.822667262107245	24.327187075285078
36-37	24.888243204458018	24.891048814387943	25.666493600307007	24.554214380847036
38-39	24.796916563249024	25.15664225064308	25.64385544406804	24.40258574203985
40-41	24.83465277889747	24.991148159494365	25.76914270972735	24.405056351880813
42-43	24.736872657847382	25.01364172885532	25.647385496552484	24.60210011674482
44-45	24.621529491061896	25.079093257481205	25.65517597241975	24.64420127903714
46-47	24.757633352050316	25.101828018129563	25.510365671355373	24.630172958464748
48-49	24.893043636132628	25.26659681892893	25.238090170656147	24.602269374282297
50-51	24.776103904814974	25.454306234908053	25.22576646440881	24.543823395868163
52-53	24.924000983004472	25.25650676073581	25.326749009416588	24.49274324684313
54-55	24.745431504000155	25.26075978756131	25.484323456288514	24.509485252150025
56-57	24.682533463734764	25.29848996821546	25.274950639963397	24.744025928086387
58-59	24.755557475997524	25.185786637034536	25.35378947986191	24.704866407106035
60-61	24.813630561460403	25.299717781809516	25.49046413333415	24.39618752339593
62-63	24.67079816832962	25.40099631982711	25.39293495803094	24.53527055381233
64-65	24.68763351492707	25.144192090595872	25.438106308917867	24.730068085559186
66-67	24.64284562754316	25.299439618760193	25.40780793561742	24.649906818079227
68-69	24.673024654937276	25.342870315739248	25.341774110865	24.642330918458477
70-71	24.530588421191805	25.067445767669245	25.585244807336966	24.816721003801987
72-73	24.79006511890641	25.422489414187776	25.229231640753337	24.55821382615248
74-75	24.601956241707253	25.528132058406726	25.30829266562519	24.561619034260833
76-77	24.66925696186166	25.166745819949014	25.306448945165638	24.857548273023692
78-79	24.560704935580805	25.278085866467197	25.382775119617225	24.778434078334772
80-81	24.602334586695235	25.47753862204127	25.14144247629837	24.778684314965126
82-83	24.76227963960897	25.38578784279895	25.265154592380828	24.586777925211255
84-85	24.554972454858948	25.391304123566773	25.260743153044213	24.79298026853007
86-87	24.34566088001282	25.536142059552752	25.35380943339234	24.764387627042094
88-89	24.49406658819714	25.309743471699548	25.332246236992805	24.863943703110507
90-91	24.475980055264504	25.28444586567089	25.386438920859323	24.853135158205284
92-93	24.549303121173807	25.496087330196836	25.250276114499993	24.704333434129364
94-95	24.528860241674604	25.21955252965325	25.364583081488217	24.887004147183927
96-97	24.534014229316288	25.216855076757138	25.397985356782165	24.85114533714441
98-99	24.40889777716746	25.289366010651104	25.259065060974212	25.04267115120722
100-101	24.391049775902708	25.371443196880687	25.3599353238193	24.877571703397305
102-103	24.43896327188181	25.10101255924163	25.549351988517046	24.910672180359516
104-105	24.289755007905313	25.36568305588192	25.34898600897063	24.995575927242133
106-107	24.249612541034548	25.31151442946184	25.458020336115815	24.9808526933878
108-109	24.21897892573639	25.36861022686062	25.351944231776606	25.060466615626385
110-111	24.243244741683974	25.434512965119055	25.425423005463	24.89681928773397
112-113	24.201303881828657	25.447905166369843	25.462636291108943	24.888154660692557
114-115	24.078967726962105	25.607488685740808	25.442448779607258	24.87109480768983
116-117	24.030044727989612	25.69796422534448	25.374205269109325	24.89778577755658
118-119	24.203412524653046	25.237128071776233	25.48970064802297	25.069758755547745
120-121	23.829966596815215	25.416666397094833	25.70877443372124	25.044592572368714
122-123	23.917435298244527	25.507864287519794	25.55616169119582	25.018538723039864
124-125	23.894011259007126	25.541668479776174	25.52539078553574	25.038929475680966
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	120.0
1	128.5
2	152.5
3	159.0
4	144.5
5	140.5
6	137.5
7	175.5
8	257.5
9	307.5
10	340.0
11	388.0
12	441.0
13	508.0
14	591.0
15	669.0
16	703.5
17	750.0
18	856.5
19	964.0
20	1035.0
21	1177.0
22	1440.0
23	1783.0
24	2107.0
25	2628.0
26	3641.5
27	4756.5
28	5658.5
29	6876.0
30	8731.0
31	10659.0
32	12842.0
33	15261.0
34	17801.0
35	20170.0
36	23412.5
37	26688.0
38	28774.0
39	31220.0
40	33536.0
41	35510.0
42	37486.0
43	41466.0
44	45466.0
45	48342.0
46	52009.0
47	55240.5
48	59165.0
49	63076.5
50	63802.0
51	63552.0
52	63518.5
53	63775.0
54	64854.5
55	64299.0
56	61271.5
57	56809.0
58	53170.0
59	49182.0
60	44030.0
61	39260.0
62	33991.0
63	26929.5
64	20388.5
65	16182.0
66	12782.5
67	9831.0
68	7762.5
69	6236.0
70	5179.0
71	4261.5
72	3456.0
73	3053.5
74	2463.0
75	1860.0
76	1325.5
77	806.0
78	483.0
79	308.0
80	177.5
81	95.0
82	70.5
83	45.5
84	27.5
85	20.0
86	14.5
87	14.0
88	11.0
89	9.5
90	9.0
91	11.0
92	9.5
93	6.5
94	6.5
95	6.0
96	7.5
97	6.5
98	6.5
99	8.0
100	27.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.037259889664312894
2	0.026301098586573805
3	0.0277192950789871
4	0.031393713263876084
5	0.034294569725630546
6	0.04428640864945148
7	0.04093430784920188
8	0.034359033202558426
9	0.03003998024839067
10-11	0.03497143623337326
12-13	0.034359033202558426
14-15	0.03310199540246482
16-17	0.03532598535647658
18-19	0.048605461603619234
20-21	0.05927416703518288
22-23	0.05337575889628214
24-25	0.06291635348160793
26-27	0.04754181423430927
28-29	0.04812198552666016
30-31	0.04873438855747499
32-33	0.055825371019541464
34-35	0.038259073556694986
36-37	0.051635245019229455
38-39	0.059177471819791064
40-41	0.04776743640355684
42-43	0.05647000578882023
44-45	0.05679232317345961
46-47	0.06320643912778337
48-49	0.048379839434371665
50-51	0.04963687723446527
52-53	0.05911300834286319
54-55	0.04518889732644176
56-57	0.04315829780321363
58-59	0.04493104341873025
60-61	0.03323092235632058
62-63	0.04251366303393486
64-65	0.041482247403088826
66-67	0.03455242363334206
68-69	0.029717662863751285
70-71	0.04905670594211438
72-73	0.03142594500234002
74-75	0.0379367561720556
76-77	0.030781310233061257
78-79	0.03132924978694821
80-81	0.02388371820177842
82-83	0.01830762744751706
84-85	0.017179516601279213
86-87	0.01640595487814469
88-89	0.022272131278581492
90-91	0.011603425847017856
92-93	0.017985310062877673
94-95	0.013762952324101733
96-97	0.011119949770058778
98-99	0.010281924569996378
100-101	0.009927375446893054
102-103	0.006285189000468005
104-105	0.006027335092756497
106-107	0.008638105908335515
108-109	0.013118317554822964
110-111	0.006704201600499205
112-113	0.008283556785232191
114-115	0.008122398092912498
116-117	0.007219909415922221
118-119	0.24431657755665373
120-121	0.36131778818075044
122-123	0.029524272432967652
124-125	0.004093430784920188
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1551266.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.45911259122945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.80438966254003	49.458208050643144
2	9.396410093039774	11.36197231536913
3	3.1479339140685054	5.709638728212521
4	1.603890720270337	3.878792385634096
5	0.9570268438029859	2.8930496851156837
6	0.6241885605378639	2.2642731875829694
7	0.4301535565358416	1.8204691614282758
8	0.3319611196869122	1.6056059768849298
9	0.24285409707233246	1.3214468878323762
>10	1.373651013252013	14.658519927596672
>50	0.0664482013366746	2.711876170559147
>100	0.02066179388600763	2.062878176298354
>500	1.0760599267584937E-4	0.039880326127549555
>1k	3.228179780275481E-4	0.21338902071511018
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	6.446347692787697E-5	6.446347692787697E-5
6	0.0	0.0	0.0	6.446347692787697E-5	1.2892695385575395E-4
7	0.0	0.0	0.0	6.446347692787697E-5	1.2892695385575395E-4
8	0.0	0.0	0.0	6.446347692787697E-5	1.2892695385575395E-4
9	0.0	0.0	0.0	6.446347692787697E-5	1.9339043078363092E-4
10-11	0.0	0.0	0.0	6.446347692787697E-5	1.9339043078363092E-4
12-13	0.0	0.0	0.0	6.446347692787697E-5	4.1901260003120036E-4
14-15	0.0	0.0	0.0	6.446347692787697E-5	6.446347692787697E-4
16-17	0.0	0.0	0.0	9.669521539181546E-5	6.446347692787697E-4
18-19	0.0	0.0	0.0	1.9339043078363092E-4	7.090982462066467E-4
20-21	0.0	0.0	0.0	1.9339043078363092E-4	7.735617231345237E-4
22-23	0.0	0.0	0.0	2.578539077115079E-4	8.057934615984621E-4
24-25	0.0	0.0	0.0	2.900856461754464E-4	8.380252000624006E-4
26-27	0.0	0.0	0.0	4.834760769590773E-4	8.380252000624006E-4
28-29	0.0	0.0	0.0	0.0011603425847017857	8.380252000624006E-4
30-31	0.0	0.0	0.0	0.0038678086156726183	8.380252000624006E-4
32-33	0.0	0.0	0.0	0.009540594585325793	8.380252000624006E-4
34-35	0.0	0.0	0.0	0.016953894432031644	9.669521539181546E-4
36-37	0.0	0.0	0.0	0.02800938072516254	9.669521539181546E-4
38-39	0.0	0.0	0.0	0.0467360207727108	9.669521539181546E-4
40-41	0.0	0.0	0.0	0.07000733594367439	9.669521539181546E-4
42-43	0.0	0.0	0.0	0.08924968380664566	9.669521539181546E-4
44-45	0.0	0.0	0.0	0.11223091333143381	0.0010958791077739084
46-47	0.0	0.0	0.0	0.13949896407192577	0.0012892695385575395
48-49	0.0	0.0	0.0	0.16818521130483102	0.0014181964924132933
50-51	0.0	0.0	0.0	0.20189960973811066	0.0014181964924132933
52-53	0.0	0.0	0.0	0.23764460769461848	0.0015148917078051088
54-55	0.0	6.446347692787697E-5	0.0	0.27322844695880655	0.0016438186616608628
56-57	0.0	9.669521539181546E-5	0.0	0.31487185305421506	0.0016760504001248012
58-59	0.0	1.2892695385575395E-4	0.0	0.3629616068424113	0.0017082821385887396
60-61	0.0	1.2892695385575395E-4	0.0	0.4130174966769078	0.0017405138770526783
62-63	0.0	1.2892695385575395E-4	0.0	0.4665866460039735	0.0018694408309084321
64-65	0.0	1.2892695385575395E-4	0.0	0.5205748079310706	0.0018694408309084321
66-67	0.0	1.2892695385575395E-4	0.0	0.5809126223355634	0.0018694408309084321
68-69	0.0	1.2892695385575395E-4	0.0	0.6494695300483605	0.0018694408309084321
70-71	0.0	1.2892695385575395E-4	0.0	0.726761238884885	0.001998367784764186
72-73	0.0	1.2892695385575395E-4	0.0	0.8082753054601854	0.0021595264770838786
74-75	0.0	1.2892695385575395E-4	0.0	0.8936249489126945	0.0021917582155478173
76-77	0.0	1.2892695385575395E-4	0.0	0.9870969904581162	0.0021917582155478173
78-79	0.0	1.2892695385575395E-4	0.0	1.0909798835273898	0.0021917582155478173
80-81	0.0	1.2892695385575395E-4	0.0	1.2076265450283832	0.002256221692475694
82-83	0.0	1.2892695385575395E-4	0.0	1.3348129850070847	0.002385148646331448
84-85	0.0	1.2892695385575395E-4	0.0	1.4656416114322108	0.002385148646331448
86-87	0.0	1.2892695385575395E-4	0.0	1.6000801925652983	0.002385148646331448
88-89	0.0	1.2892695385575395E-4	0.0	1.7507635698842106	0.002449612123259325
90-91	0.0	1.2892695385575395E-4	0.0	1.9208826854968781	0.002481843861723263
92-93	0.0	1.2892695385575395E-4	0.0	2.1034432521566258	0.002578539077115079
94-95	0.0	1.2892695385575395E-4	0.0	2.2890658339704473	0.002643002554042956
96-97	0.0	1.2892695385575395E-4	0.0	2.4851637307850494	0.002707466030970833
98-99	0.0	1.2892695385575395E-4	0.0	2.7020510989088913	0.002707466030970833
100-101	0.0	1.2892695385575395E-4	0.0	2.949655313788867	0.002707466030970833
102-103	0.0	1.2892695385575395E-4	0.0	3.2189514886550725	0.00277192950789871
104-105	0.0	1.2892695385575395E-4	0.0	3.4984006611374197	0.0028363929848265867
106-107	0.0	1.9339043078363092E-4	0.0	3.7905813703130216	0.0028363929848265867
108-109	0.0	1.9339043078363092E-4	0.0	4.094623359243354	0.0028363929848265867
110-111	0.0	1.9339043078363092E-4	0.0	4.435377298284111	0.0028686247232905253
112-113	0.0	1.9339043078363092E-4	0.0	4.801336456803669	0.002900856461754464
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1660	0.0	72.05809	1
GTATCAA	3205	0.0	55.332848	1
TCAACGC	4045	0.0	42.94425	4
CAACGCA	4200	0.0	41.642685	5
AACGCAG	4420	0.0	39.303326	6
ATCAACG	4490	0.0	38.820576	3
GTACATG	8085	0.0	37.76001	1
TATCAAC	4715	0.0	37.094227	2
TACATGG	8305	0.0	36.24529	2
ACATGGG	8485	0.0	35.616608	3
CATGGGG	6905	0.0	33.16941	4
ACGCAGA	5240	0.0	33.039265	7
CGCAGAG	5670	0.0	30.320856	8
ATGGGGG	3850	0.0	28.58589	5
ATGGGTA	940	0.0	28.479042	5
GCAGAGT	6125	0.0	27.195223	9
GGTACCT	825	0.0	26.679302	8
TACCGTA	140	1.0569507E-4	25.497166	7
CATGGGT	1485	0.0	25.237993	4
AGAGTAC	5335	0.0	25.145065	10-11
>>END_MODULE