FastQCFastQC Report
Mon 15 Aug 2016
SRR2921509_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921509_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1551266
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26150.1685719921663983No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23690.15271397684214055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24250.076.450061
GTATCAA46400.057.4273451
TCAACGC61600.042.4307024
CAACGCA62100.042.0632865
AACGCAG64650.040.4936036
ATCAACG67200.039.072013
TATCAAC67900.038.581522
GTACATG77400.036.121111
ACGCAGA77250.033.809627
TACATGG81800.033.699552
ACATGGG80800.033.5271383
CGCAGAG82800.031.5423778
CATGGGG67400.031.005854
GCAGAGT88050.028.9184279
ATGGGGG38150.027.9182875
GTGGTAT9350.026.7773061
AGAGTAC83650.025.46152110-11
CAGAGTA87300.025.1806810-11
GAGTACT48950.023.63889312-13
GTACTTT51650.022.34702314-15
Produced by FastQC (version 0.11.5)