##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921509_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1551266
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.163552865852793	31.0	31.0	34.0	28.0	34.0
2	31.215375054955114	31.0	31.0	34.0	28.0	34.0
3	31.29872697525763	33.0	31.0	34.0	28.0	34.0
4	34.84228945906118	37.0	35.0	37.0	32.0	37.0
5	34.48693389786149	37.0	35.0	37.0	30.0	37.0
6	34.46513363923402	37.0	35.0	37.0	30.0	37.0
7	34.29857935389546	37.0	35.0	37.0	30.0	37.0
8	34.261431630681	37.0	35.0	37.0	30.0	37.0
9	35.81581817689552	39.0	37.0	39.0	30.0	39.0
10-11	35.681465009869356	39.0	35.0	39.0	30.0	39.0
12-13	35.59937786298417	39.0	35.0	39.0	30.0	39.0
14-15	36.75633998295586	40.0	36.5	41.0	30.0	41.0
16-17	36.729725591871414	40.0	36.0	41.0	30.0	41.0
18-19	36.750079934711394	40.0	36.5	41.0	30.0	41.0
20-21	36.727964771999126	40.0	37.0	41.0	30.0	41.0
22-23	36.66933298351154	40.0	36.5	41.0	30.0	41.0
24-25	36.59980171034497	40.0	36.0	41.0	29.5	41.0
26-27	36.47357674312465	40.0	36.0	41.0	29.0	41.0
28-29	36.28042031476227	40.0	36.0	41.0	28.5	41.0
30-31	36.22288376074768	40.0	36.0	41.0	29.0	41.0
32-33	36.09235940193365	40.0	35.0	41.0	28.0	41.0
34-35	35.962331089574576	40.0	35.0	41.0	28.0	41.0
36-37	35.82384097891658	40.0	35.0	41.0	27.0	41.0
38-39	35.65140246740404	39.5	35.0	41.0	26.5	41.0
40-41	35.442841201960206	39.0	35.0	41.0	26.0	41.0
42-43	35.26946603612791	39.0	35.0	41.0	25.5	41.0
44-45	35.04898482916534	39.0	35.0	41.0	24.5	41.0
46-47	34.765479937032076	39.0	34.0	41.0	23.5	41.0
48-49	34.500709098246205	39.0	34.0	41.0	22.5	41.0
50-51	34.2730585856971	38.5	34.0	41.0	22.0	41.0
52-53	34.074129452975825	38.0	33.5	40.5	21.0	41.0
54-55	33.854758629403335	38.0	33.0	40.0	20.0	41.0
56-57	33.59260597473289	37.5	33.0	40.0	19.5	41.0
58-59	33.44942646844577	37.0	33.0	40.0	19.5	41.0
60-61	33.3290821819082	37.0	33.0	40.0	19.5	41.0
62-63	33.05868110304745	36.0	33.0	40.0	18.0	41.0
64-65	32.76023261001015	36.0	33.0	39.0	18.0	41.0
66-67	32.4579514409521	35.0	32.0	39.0	16.5	41.0
68-69	32.144245409878124	35.0	32.0	38.5	14.5	40.5
70-71	31.846171127324393	35.0	32.0	37.5	13.0	40.0
72-73	31.532655908142125	35.0	32.0	37.0	12.0	39.0
74-75	31.247805018610606	35.0	32.0	36.5	10.5	39.0
76-77	30.963188131500335	35.0	31.0	36.0	8.0	39.0
78-79	30.689148411684393	35.0	31.0	35.5	7.0	37.0
80-81	30.467470440272656	35.0	31.0	35.0	7.0	37.0
82-83	30.28060887043228	35.0	31.0	35.0	6.5	36.5
84-85	30.08878232359892	35.0	31.0	35.0	3.5	36.0
86-87	29.94738394317931	35.0	31.0	35.0	2.0	36.0
88-89	29.844209503721476	35.0	31.0	35.0	2.0	36.0
90-91	29.74469562280099	35.0	31.0	35.0	2.0	35.0
92-93	29.802020091976487	35.0	31.0	35.0	2.0	35.0
94-95	29.768538729012306	35.0	31.0	35.0	2.0	35.0
96-97	29.746341053049576	35.0	31.0	35.0	2.0	35.0
98-99	29.70824055964612	35.0	31.0	35.0	2.0	35.0
100-101	29.65910681984908	35.0	31.0	35.0	2.0	35.0
102-103	28.768194816362893	33.5	29.0	35.0	2.0	35.0
104-105	29.475862940333897	34.5	30.5	35.0	2.0	35.0
106-107	29.581262014380513	35.0	31.0	35.0	2.0	35.0
108-109	29.588140267368715	35.0	31.0	35.0	2.0	35.0
110-111	29.552902274658248	35.0	31.0	35.0	2.0	35.0
112-113	29.503879734358904	35.0	31.0	35.0	2.0	35.0
114-115	29.4408415449059	35.0	31.0	35.0	2.0	35.0
116-117	29.35923207238475	35.0	31.0	35.0	2.0	35.0
118-119	29.263314286524682	35.0	31.0	35.0	2.0	35.0
120-121	29.13929010240668	35.0	31.0	35.0	2.0	35.0
122-123	29.013429031513617	35.0	31.0	35.0	2.0	35.0
124-125	28.092731033878138	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	26159.0
3	9692.0
4	5579.0
5	4781.0
6	5000.0
7	5941.0
8	6615.0
9	7648.0
10	8751.0
11	9407.0
12	9923.0
13	11285.0
14	14340.0
15	11789.0
16	6816.0
17	5844.0
18	5329.0
19	4752.0
20	4556.0
21	4769.0
22	5826.0
23	7678.0
24	10692.0
25	14538.0
26	18570.0
27	20580.0
28	24133.0
29	29144.0
30	33573.0
31	40257.0
32	50508.0
33	64110.0
34	89074.0
35	138999.0
36	271592.0
37	427186.0
38	135528.0
39	302.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.86047116515952	17.82898205942585	12.410021214759587	24.900525560655044
2	17.206728543966708	19.88509852105603	37.07016700166705	25.83800593331021
3	18.989138949872817	24.694204538494024	29.302462031458038	27.014194480175117
4	13.465779250641278	15.686082278403452	35.47299149455728	35.37514697639799
5	14.240504900769176	37.25558215695744	33.44251872647705	15.06139421579633
6	33.345691230943316	35.23947993668458	16.73197009603698	14.682858736335119
7	29.287636029082293	31.648363976075515	20.719036882828178	18.344963112014014
8	28.148000590517043	33.17970864817369	19.28121955952328	19.39107120178599
9	27.903285697340397	14.062717572369404	18.803409987570976	39.23058674271922
10-11	26.47206245884143	24.774274569670354	26.83311355235789	21.920549419130325
12-13	26.802776564557384	22.526306289173366	27.225288849421176	23.44562829684808
14-15	24.27697558126567	23.51061450065013	24.7639449287564	27.448464989327803
16-17	24.078655600659268	26.459069966698184	25.19046764056729	24.271806792075257
18-19	24.085749705548455	25.27644922701231	27.343766620315122	23.29403444712411
20-21	25.168148587745275	24.26249134878941	27.162786529545315	23.40657353392
22-23	24.942469490859427	23.898781117163676	27.041837469179796	24.1169119227971
24-25	24.357843620794256	24.442076176145004	27.062859471214257	24.137220731846483
26-27	24.062705395078996	24.499259086863646	27.17338012083476	24.264655397222597
28-29	24.206607094503113	24.560139517491265	27.15536471832166	24.077888669683958
30-31	24.488384006251678	24.43412671061196	27.01265391034554	24.06483537279082
32-33	24.16231241584441	24.54769141699233	26.980333904874087	24.309662262289176
34-35	24.36548485184545	24.610786435135157	27.02901709148211	23.994711621537277
36-37	24.574092221992984	24.38067361254384	26.821680936661853	24.223553228801322
38-39	24.515562885832196	24.696645506510688	26.697194202648124	24.090597405008996
40-41	24.569358726646666	24.545053661329224	26.77460823393722	24.11097937808689
42-43	24.4501829150951	24.676501865253677	26.593943316789474	24.279371902861755
44-45	24.40725491359145	24.708446915675268	26.472143205304434	24.412154965428844
46-47	24.558226769188316	24.799615995130953	26.144600141068274	24.497557094612453
48-49	24.797132337319816	25.029635071934962	25.635038407117705	24.538194183627514
50-51	24.680446940701152	25.246553346526685	25.53781075650526	24.5351889562669
52-53	24.88002460438585	25.14902224001785	25.573119063692218	24.397834091904087
54-55	24.64035357776664	25.141061990551272	25.770203465178167	24.448380966503922
56-57	24.602196198079504	25.218881552181145	25.565603857757136	24.61331839198222
58-59	24.62692052275614	24.981560402576385	25.753347818518257	24.638171256149217
60-61	24.680239914762883	25.160198392293843	25.85141210244573	24.308149590497543
62-63	24.61487276979216	25.223693759012626	25.798309872958384	24.36312359823682
64-65	24.612668246726617	24.89242396498766	25.84955086924789	24.64535691903783
66-67	24.53630353514661	25.12740189111502	25.76630516947538	24.569989404262987
68-69	24.642338552641423	25.164452050024888	25.61934686003296	24.57386253730073
70-71	24.50833179673588	24.92733935679535	25.8876268015262	24.676702044942576
72-73	24.761619843854472	25.258464115477285	25.48012913050963	24.499786910158612
74-75	24.593646638598575	25.426114933042765	25.388106951044172	24.59213147731449
76-77	24.60682976833846	25.077309160889765	25.46416926298009	24.85169180779168
78-79	24.542156310542197	25.21515098855161	25.498483231070857	24.744209469835337
80-81	24.604364092388387	25.382273151498836	25.28017460238144	24.733188153731337
82-83	24.796051910542637	25.20107921321875	25.326084854267535	24.67678402197108
84-85	24.535613296043138	25.294472618398302	25.24167063165102	24.92824345390754
86-87	24.40084470202849	25.38206455568525	25.39428185338018	24.82280888890608
88-89	24.458953798421422	25.168871922981573	25.38328840935592	24.988885869241084
90-91	24.446917275006623	25.136727400732006	25.47671653087947	24.939638793381896
92-93	24.532955804061277	25.308998941422363	25.340653120483154	24.817392134033206
94-95	24.476291783515457	25.061373819424297	25.40534442188054	25.056989975179704
96-97	24.410545743584255	25.068832785486034	25.443859457988953	25.076762012940755
98-99	24.354524236908613	25.078139126807947	25.287810167288804	25.279526468994636
100-101	24.349135124331923	25.179357741975	25.38756616314978	25.083940970543296
102-103	24.408665333733452	24.907242094989382	25.53916589925771	25.14492667201946
104-105	24.338339362421873	25.13886565463073	25.310181375483126	25.212613607464274
106-107	24.38667638779032	25.0457208683774	25.384026285712075	25.1835764581202
108-109	24.40546173449239	24.98906331350169	25.27385237326903	25.33162257873689
110-111	24.38445786942117	25.138202661136518	25.309805708863376	25.167533760578937
112-113	24.323221383051767	25.03827842192975	25.406718306272502	25.231781888745985
114-115	24.131868004355244	25.246239604237402	25.411624172513093	25.21026821889426
116-117	24.164315436693755	25.22582606259854	25.286297774183286	25.32356072652442
118-119	24.1411167335971	24.8972189647047	25.39341105386906	25.568253247829137
120-121	23.861151858989672	25.04339367027773	25.597317699041167	25.49813677169144
122-123	23.984756848330445	25.098887738565058	25.395196402394447	25.521159010710043
124-125	23.977149850375945	25.116304411604457	25.359358870117553	25.547186867902045
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	20.0
1	26.0
2	66.0
3	97.0
4	75.5
5	66.5
6	128.0
7	263.0
8	404.0
9	485.5
10	444.0
11	398.0
12	456.5
13	549.0
14	656.0
15	800.0
16	960.5
17	1094.5
18	1265.5
19	1476.0
20	1637.5
21	1848.0
22	2155.5
23	2498.5
24	2879.0
25	3482.0
26	4433.0
27	5398.0
28	6215.5
29	7439.5
30	9264.5
31	11016.5
32	13072.0
33	15431.5
34	18034.0
35	20536.5
36	23278.0
37	26286.5
38	28428.5
39	30534.0
40	32636.5
41	34684.0
42	36796.0
43	40695.5
44	44923.0
45	47967.5
46	51674.5
47	54933.0
48	59104.0
49	63052.5
50	63864.5
51	63959.0
52	63714.5
53	64196.0
54	65505.0
55	64675.5
56	61595.0
57	56978.5
58	53145.5
59	49588.5
60	44498.5
61	39059.5
62	33400.5
63	26261.5
64	19774.5
65	15570.5
66	11976.0
67	9080.0
68	7133.0
69	5750.0
70	4874.5
71	3940.0
72	3128.0
73	2797.0
74	2290.5
75	1748.0
76	1189.5
77	673.5
78	388.5
79	225.0
80	117.0
81	47.0
82	29.5
83	17.0
84	6.5
85	4.5
86	3.0
87	2.5
88	1.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12074009228591356
2	0.0015471234462690474
3	0.020048141324569738
4	0.054600564957911796
5	0.024947365571088388
6	0.019403506555290967
7	0.014117501447205057
8	0.005350468585013789
9	0.0037388816618168642
10-11	0.006478579431251635
12-13	0.004415748169559573
14-15	0.0025140756001872018
16-17	0.009637289800717607
18-19	0.004963687723446527
20-21	0.011764584539337548
22-23	0.008541410692943698
24-25	0.013086085816359025
26-27	0.008992655031438836
28-29	0.013279476247142655
30-31	0.020789471309240325
32-33	0.012602609739399947
34-35	0.020499385663064876
36-37	0.014568745785700197
38-39	0.020402690447673063
40-41	0.009605058062253669
42-43	0.008799264600655207
44-45	0.016889430955103767
46-47	0.015729088370401982
48-49	0.020402690447673063
50-51	0.014987758385731395
52-53	0.02082170304770426
54-55	0.016986126170495584
56-57	0.020177068278425494
58-59	0.016502650093536506
60-61	0.019242347862971278
62-63	0.02046715392460094
64-65	0.017405138770526785
66-67	0.010991022816203025
68-69	0.023077924740179955
70-71	0.014181964924132935
72-73	0.01785638310902192
74-75	0.017791919632094044
76-77	0.012151365400904808
78-79	0.0281383076790183
80-81	0.02020930001688943
82-83	0.008767032862191269
84-85	0.012119133662440872
86-87	0.012022438447049056
88-89	0.020144836539961554
90-91	0.012054670185512994
92-93	0.008251325046768253
94-95	0.0074777633236337284
96-97	0.00277192950789871
98-99	0.0017082821385887396
100-101	0.011345571939306347
102-103	0.003255405584857787
104-105	0.0023529169078675095
106-107	3.223173846393849E-4
108-109	0.018920030478331892
110-111	7.413299846705852E-4
112-113	0.008444715477551884
114-115	0.00212729473861994
116-117	0.008219093308304313
118-119	0.009862911969965176
120-121	0.0039000403541365565
122-123	7.735617231345237E-4
124-125	0.024431657755665373
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1551266.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.14706669963746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.95166970917134	45.69003401617398
2	10.240494940201613	11.70428494770003
3	3.511143981063366	6.019547378335763
4	1.764482112278754	4.033399078428445
5	1.0308623352991935	2.9455379316743526
6	0.6944902578018658	2.3812848650910983
7	0.4878784688246091	1.951657637946585
8	0.3680907268080378	1.6828244253133575
9	0.2798376023152585	1.439270831211903
>10	1.5638215024557862	15.99254298724538
>50	0.07970926695297326	3.056822560104983
>100	0.02695289607533394	2.6060530961870723
>500	2.264803007155472E-4	0.09402860544357079
>1k	3.397204510733208E-4	0.40271163914338676
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2615	0.1685719921663983	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2369	0.15271397684214055	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.446347692787697E-5
2	0.0	0.0	0.0	0.0	6.446347692787697E-5
3	0.0	0.0	0.0	0.0	6.446347692787697E-5
4	0.0	0.0	0.0	0.0	6.446347692787697E-5
5	0.0	0.0	0.0	6.446347692787697E-5	6.446347692787697E-5
6	0.0	0.0	0.0	6.446347692787697E-5	6.446347692787697E-5
7	0.0	0.0	0.0	6.446347692787697E-5	6.446347692787697E-5
8	0.0	0.0	0.0	6.446347692787697E-5	6.446347692787697E-5
9	0.0	0.0	0.0	6.446347692787697E-5	6.446347692787697E-5
10-11	0.0	0.0	0.0	6.446347692787697E-5	6.446347692787697E-5
12-13	0.0	0.0	0.0	6.446347692787697E-5	2.578539077115079E-4
14-15	0.0	0.0	0.0	6.446347692787697E-5	4.1901260003120036E-4
16-17	0.0	0.0	0.0	9.669521539181546E-5	4.512443384951388E-4
18-19	0.0	0.0	0.0	1.6115869231969244E-4	4.512443384951388E-4
20-21	0.0	0.0	0.0	2.256221692475694E-4	5.479395538869542E-4
22-23	0.0	0.0	0.0	3.223173846393849E-4	5.801712923508927E-4
24-25	0.0	0.0	0.0	3.5454912310332334E-4	5.801712923508927E-4
26-27	0.0	0.0	0.0	5.479395538869543E-4	7.090982462066467E-4
28-29	0.0	0.0	0.0	0.001192574323165724	7.735617231345237E-4
30-31	0.0	0.0	0.0	0.003964503831064434	7.735617231345237E-4
32-33	0.0	0.0	0.0	0.00983068023150124	7.735617231345237E-4
34-35	0.0	0.0	0.0	0.01724398007820709	7.735617231345237E-4
36-37	0.0	0.0	0.0	0.02916972330986433	7.735617231345237E-4
38-39	0.0	0.0	0.0	0.04963687723446527	8.380252000624006E-4
40-41	0.0	0.0	0.0	0.07371398586702732	8.380252000624006E-4
42-43	0.0	0.0	0.0	0.09405221283777251	8.380252000624006E-4
44-45	0.0	0.0	0.0	0.11793593103955093	8.702569385263391E-4
46-47	0.0	0.0	0.0	0.14642878784167254	9.024886769902776E-4
48-49	0.0	0.0	0.0	0.17604975549003202	9.024886769902776E-4
50-51	0.0	0.0	0.0	0.21073110607722984	9.024886769902776E-4
52-53	0.0	0.0	0.0	0.2476364466184394	0.00106364736930997
54-55	0.0	0.0	0.0	0.28467071411350475	0.0011603425847017854
56-57	0.0	0.0	0.0	0.32740999931668713	0.0011603425847017854
58-59	0.0	0.0	0.0	0.37659563221265724	0.0011925743231657238
60-61	0.0	0.0	0.0	0.42893997547809337	0.0013537330154854165
62-63	0.0	0.0	0.0	0.4844107973745315	0.0013537330154854165
64-65	0.0	0.0	0.0	0.5400427779632894	0.0014181964924132933
66-67	0.0	0.0	0.0	0.6020888745063709	0.001450428230877232
68-69	0.0	0.0	0.0	0.6727086134808602	0.0014826599693411704
70-71	0.0	0.0	0.0	0.7523210074867882	0.0014826599693411704
72-73	0.0	0.0	0.0	0.8361879909699561	0.0016438186616608628
74-75	0.0	0.0	0.0	0.9241484052380442	0.0016760504001248012
76-77	0.0	0.0	0.0	1.0200700589067253	0.0016760504001248012
78-79	0.0	0.0	0.0	1.128562090576342	0.0016760504001248012
80-81	0.0	0.0	0.0	1.2495278050315033	0.0017082821385887396
82-83	0.0	0.0	0.0	1.380098577548918	0.0017405138770526783
84-85	0.0	0.0	0.0	1.514375999989686	0.0017405138770526783
86-87	0.0	0.0	0.0	1.6539071958000755	0.001772745615516617
88-89	0.0	0.0	0.0	1.8097798830116822	0.0018049773539805553
90-91	0.0	0.0	0.0	1.9862486511017452	0.0018372090924444937
92-93	0.0	0.0	0.0	2.173547283315692	0.001998367784764186
94-95	0.0	0.0	0.0	2.365293895437662	0.001998367784764186
96-97	0.0	3.223173846393849E-5	0.0	2.568934019052825	0.0020950630001560014
98-99	0.0	6.446347692787697E-5	0.0	2.792428893561775	0.00212729473861994
100-101	0.0	6.446347692787697E-5	0.0	3.0491869221655086	0.00212729473861994
102-103	0.0	6.446347692787697E-5	0.0	3.32621871426306	0.0021917582155478173
104-105	0.0	6.446347692787697E-5	0.0	3.614499383084526	0.0021917582155478173
106-107	0.0	6.446347692787697E-5	0.0	3.915447125122319	0.0021917582155478173
108-109	0.0	6.446347692787697E-5	0.0	4.230705759038102	0.0021917582155478173
110-111	0.0	6.446347692787697E-5	0.0	4.584223466510579	0.002256221692475694
112-113	0.0	6.446347692787697E-5	0.0	4.961850514354083	0.002256221692475694
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2425	0.0	76.45006	1
GTATCAA	4640	0.0	57.427345	1
TCAACGC	6160	0.0	42.430702	4
CAACGCA	6210	0.0	42.063286	5
AACGCAG	6465	0.0	40.493603	6
ATCAACG	6720	0.0	39.07201	3
TATCAAC	6790	0.0	38.58152	2
GTACATG	7740	0.0	36.12111	1
ACGCAGA	7725	0.0	33.80962	7
TACATGG	8180	0.0	33.69955	2
ACATGGG	8080	0.0	33.527138	3
CGCAGAG	8280	0.0	31.542377	8
CATGGGG	6740	0.0	31.00585	4
GCAGAGT	8805	0.0	28.918427	9
ATGGGGG	3815	0.0	27.918287	5
GTGGTAT	935	0.0	26.777306	1
AGAGTAC	8365	0.0	25.461521	10-11
CAGAGTA	8730	0.0	25.18068	10-11
GAGTACT	4895	0.0	23.638893	12-13
GTACTTT	5165	0.0	22.347023	14-15
>>END_MODULE