FastQCFastQC Report
Mon 15 Aug 2016
SRR2921508_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921508_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1520702
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17750.069.749651
GTATCAA34100.058.125531
TCAACGC44600.042.961924
CAACGCA46300.041.5130125
AACGCAG47450.041.0017856
ATCAACG48850.039.343423
TATCAAC50100.038.599332
GTACATG84100.035.2460441
ACGCAGA56650.034.130777
TACATGG88550.032.9262052
CGCAGAG59450.032.2198758
ACATGGG89000.032.0911563
CATGGGG70800.029.3328914
GCAGAGT64400.029.2814949
AGAGTAC58600.025.98650610-11
CAGAGTA61050.024.94363810-11
TACACCG2451.61981E-824.288275
ATGGGGG40350.024.0384675
ACCGTAT2001.8300525E-623.7946768
GTCCTAA5250.022.6750241
Produced by FastQC (version 0.11.5)