FastQCFastQC Report
Mon 15 Aug 2016
SRR2921508_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921508_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1520702
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28680.18859710844070698No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25960.1707106323263861No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24600.083.153311
GTATCAA49850.059.936731
TCAACGC66950.044.3781744
CAACGCA67950.043.7881975
TATCAAC70050.042.5020562
ATCAACG69950.042.4748953
AACGCAG71250.041.9968576
GTACATG85100.035.6003841
ACGCAGA83950.035.288087
CGTATAC851.7037525E-435.0243873
TACATGG88450.033.862442
CGCAGAG88050.033.644918
ACATGGG91650.032.093273
GCAGAGT93800.030.8204179
CATGGGG75400.030.2445344
TACCGTA1505.305119E-627.7604267
AGAGTAC87550.027.51718310-11
CAGAGTA91950.026.5238910-11
GAGTACT48550.025.66846512-13
ATGGGGG41600.025.6056735
Produced by FastQC (version 0.11.5)