##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921508_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1520702 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.326032976875155 31.0 31.0 34.0 28.0 34.0 2 31.378222031666954 33.0 31.0 34.0 28.0 34.0 3 31.461183058876756 33.0 31.0 34.0 28.0 34.0 4 34.98974552542181 37.0 35.0 37.0 32.0 37.0 5 34.66097105152752 37.0 35.0 37.0 32.0 37.0 6 34.63627193230495 37.0 35.0 37.0 31.0 37.0 7 34.48560862022934 37.0 35.0 37.0 30.0 37.0 8 34.44846722105975 37.0 35.0 37.0 30.0 37.0 9 36.03752345956013 39.0 37.0 39.0 30.0 39.0 10-11 35.90556137888949 39.0 37.0 39.0 30.0 39.0 12-13 35.82928443574086 39.0 37.0 39.0 30.0 39.0 14-15 37.00623725095383 40.0 37.0 41.0 30.0 41.0 16-17 36.979721865296426 40.0 37.0 41.0 30.0 41.0 18-19 37.00637172832021 40.0 37.0 41.0 30.0 41.0 20-21 36.98918229870152 40.0 37.0 41.0 30.0 41.0 22-23 36.931147259620886 40.0 37.0 41.0 30.0 41.0 24-25 36.86419890287512 40.0 37.0 41.0 30.0 41.0 26-27 36.73383213805203 40.0 36.5 41.0 30.0 41.0 28-29 36.54468824266688 40.0 36.0 41.0 29.5 41.0 30-31 36.4862221526637 40.0 36.0 41.0 30.0 41.0 32-33 36.35627591730662 40.0 35.5 41.0 29.5 41.0 34-35 36.22718619427081 40.0 35.0 41.0 29.0 41.0 36-37 36.08832006533825 40.0 35.0 41.0 28.5 41.0 38-39 35.91223494149413 40.0 35.0 41.0 27.5 41.0 40-41 35.711750559938764 40.0 35.0 41.0 27.0 41.0 42-43 35.536180329873964 39.0 35.0 41.0 26.5 41.0 44-45 35.31127959324049 39.0 35.0 41.0 25.5 41.0 46-47 35.017670128664264 39.0 35.0 41.0 24.0 41.0 48-49 34.75405174715362 39.0 34.0 41.0 23.0 41.0 50-51 34.52041885918477 39.0 34.0 41.0 23.0 41.0 52-53 34.32444818248415 38.5 34.0 41.0 22.5 41.0 54-55 34.10953428087817 38.0 34.0 40.0 22.0 41.0 56-57 33.85233037110493 38.0 33.0 40.0 21.5 41.0 58-59 33.6999941474398 37.0 33.0 40.0 21.5 41.0 60-61 33.57467538018626 37.0 33.0 40.0 21.0 41.0 62-63 33.30277365322068 36.5 33.0 40.0 20.0 41.0 64-65 33.00360688681938 36.0 33.0 39.0 20.0 41.0 66-67 32.700437692591976 35.5 33.0 39.0 19.0 41.0 68-69 32.38241976403003 35.0 33.0 39.0 18.0 41.0 70-71 32.076383144100554 35.0 32.0 37.5 18.0 40.0 72-73 31.76013084746387 35.0 32.0 37.0 16.5 39.5 74-75 31.47175712269728 35.0 32.0 36.5 14.5 39.0 76-77 31.186387273772244 35.0 32.0 36.0 13.0 39.0 78-79 30.91202155320372 35.0 32.0 36.0 11.0 37.0 80-81 30.69196824887453 35.0 31.0 35.0 9.0 37.0 82-83 30.500832181453042 35.0 31.0 35.0 7.5 36.5 84-85 30.31517154577294 35.0 31.0 35.0 7.0 36.0 86-87 30.178147329325533 35.0 31.0 35.0 7.0 36.0 88-89 30.071083289165134 35.0 31.0 35.0 7.0 36.0 90-91 29.974960577417534 35.0 31.0 35.0 5.5 35.0 92-93 30.03676295552975 35.0 31.0 35.0 2.0 35.0 94-95 30.005854532972272 35.0 31.0 35.0 2.0 35.0 96-97 29.988219585428308 35.0 31.0 35.0 2.0 35.0 98-99 29.94819596475838 35.0 31.0 35.0 2.0 35.0 100-101 29.90210277884819 35.0 31.0 35.0 2.0 35.0 102-103 29.01866868064881 33.5 29.5 35.0 2.0 35.0 104-105 29.72033869883777 34.5 31.0 35.0 2.0 35.0 106-107 29.82518994517006 35.0 31.0 35.0 2.0 35.0 108-109 29.828901060168263 35.0 31.0 35.0 2.0 35.0 110-111 29.79383797746041 35.0 31.0 35.0 2.0 35.0 112-113 29.74752121059879 35.0 31.0 35.0 2.0 35.0 114-115 29.680770788754142 35.0 31.0 35.0 2.0 35.0 116-117 29.598620571288787 35.0 31.0 35.0 2.0 35.0 118-119 29.50147135993771 35.0 31.0 35.0 2.0 35.0 120-121 29.376113466017667 35.0 31.0 35.0 2.0 35.0 122-123 29.253672974718256 35.0 31.0 35.0 2.0 35.0 124-125 28.33102935354856 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22846.0 3 8588.0 4 5081.0 5 4081.0 6 4503.0 7 5503.0 8 6345.0 9 7136.0 10 8262.0 11 9185.0 12 9546.0 13 11050.0 14 14110.0 15 11622.0 16 6506.0 17 5385.0 18 4937.0 19 4483.0 20 4260.0 21 4524.0 22 5406.0 23 7006.0 24 9800.0 25 13400.0 26 17319.0 27 19145.0 28 22367.0 29 27331.0 30 31663.0 31 38023.0 32 47344.0 33 60633.0 34 84694.0 35 134283.0 36 266375.0 37 436723.0 38 140990.0 39 247.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.64299235254032 17.80748680706213 12.761019354031625 24.788501486365927 2 16.838410235434456 20.018439143592527 37.68503259730199 25.458118023671023 3 18.736335149612845 24.780309986555498 29.52015491434721 26.96319994948445 4 13.410995272513135 15.807439623380171 35.62005086078423 35.161514243322465 5 14.137787682568417 37.5318697163365 33.350918137256194 14.979424463838889 6 32.94202765098199 35.26881437535353 17.00837948407635 14.780778489588128 7 29.06051767058104 32.07518752486904 20.6951820660127 18.169112738537223 8 27.89972445268675 33.196020018282134 19.49947718349873 19.404778345532385 9 28.180174137205388 14.041587752525253 18.916377314814813 38.86186079545455 10-11 26.360105735308576 24.848619564977923 26.876385770124024 21.91488892958948 12-13 26.773195862729317 22.680906948236768 27.260564139795978 23.28533304923794 14-15 24.304854559192663 23.522197371786987 24.985844896896268 27.187103172124083 16-17 23.928824630423946 26.56801001758602 25.379572595272954 24.123592756717084 18-19 23.960821024962655 25.34746712095759 27.573874063164723 23.117837790915036 20-21 25.11667963929196 24.278967017305714 27.494862741732295 23.109490601670032 22-23 24.980525119718312 23.8874984914983 27.193476564542333 23.938499824241056 24-25 24.333320398846944 24.37383384095712 27.265908267307164 24.026937492888774 26-27 23.95317483805202 24.550787543980796 27.430765183650653 24.065272434316533 28-29 24.12695447890866 24.610978623883742 27.391445607379765 23.87062128982783 30-31 24.41884594982039 24.503529924027788 27.106797508613923 23.970826617537902 32-33 24.142410061450324 24.5793397091016 27.11420249898635 24.16404773046173 34-35 24.36706029995409 24.692870006301114 27.197562955235778 23.742506738509018 36-37 24.551475165517726 24.43733181449124 26.97081435739858 24.040378662592452 38-39 24.479498276723934 24.70063011392039 26.823877239601146 23.99599436975453 40-41 24.425818684153498 24.57132672754219 26.889294521104258 24.113560067200055 42-43 24.469336753148532 24.607806385847226 26.657755417447632 24.26510144355661 44-45 24.36089310701466 24.69043966397498 26.56893067551947 24.37973655349089 46-47 24.529540366265287 24.770952652805658 26.229489328005005 24.470017652924046 48-49 24.739117351561383 25.074069262859144 25.75462840398785 24.43218498159162 50-51 24.604666687493733 25.314617475332263 25.647839548293856 24.432876288880156 52-53 24.739015944668058 25.1122191151191 25.743849366412963 24.404915573799872 54-55 24.528512850160332 25.10223269295465 25.898652912649293 24.47060154423572 56-57 24.48258364019465 25.17452206425227 25.642402670671842 24.700491624881238 58-59 24.538216492434902 24.92928839843032 25.864874988202402 24.667620120932376 60-61 24.683007713840713 25.105795641101196 25.947326464893077 24.26387018016501 62-63 24.51215309066178 25.101325086131254 25.8821629699399 24.504358853267068 64-65 24.522036819015923 24.868193447819998 25.995685374339818 24.614084358824265 66-67 24.47217285075164 25.07871575350912 25.87197324568687 24.57713815005237 68-69 24.514485916301258 25.07334797278076 25.741759226584644 24.670406884333346 70-71 24.41888799406236 24.872728372866654 25.940129637674175 24.76825399539681 72-73 24.69709067614123 25.180936608388986 25.60792332655013 24.514049388919656 74-75 24.521037138716302 25.340627054359903 25.487233740979725 24.65110206594407 76-77 24.46714125426346 25.0366332166595 25.547294995389606 24.948930533687435 78-79 24.477730833466534 25.08197713388864 25.60271693764344 24.837575095001384 80-81 24.494455112573334 25.2712754079696 25.41305062042688 24.821218859030186 82-83 24.542714506405254 25.146443817331765 25.522294907957587 24.788546768305395 84-85 24.37537940246619 25.245168804912648 25.358488059386392 25.02096373323477 86-87 24.19364650830893 25.34612734934668 25.490224448825305 24.970001693519084 88-89 24.287735866120354 25.055602857311047 25.507726519775808 25.14893475679279 90-91 24.232543074036865 25.06086021470017 25.608906324759907 25.097690386503057 92-93 24.356338688600708 25.13989920812017 25.514465403841612 24.98929669943751 94-95 24.28073172889085 24.968688151083413 25.552967745096396 25.197612374929346 96-97 24.225622288794348 24.965171292793606 25.556396201245974 25.252810217166072 98-99 24.0834449619805 24.975454849746853 25.477438518755257 25.46366166951739 100-101 24.097232201548895 25.052079753792245 25.593939379988683 25.25674866467018 102-103 24.137195188113513 24.78599626081198 25.66894047352088 25.407868077553626 104-105 24.049842946122283 25.059341146406172 25.457228506240558 25.433587401230984 106-107 24.102072931030968 24.861560192464378 25.509453888578726 25.526912987925932 108-109 24.080189136864092 24.868116895737614 25.491842338151532 25.55985162924676 110-111 24.036709736030403 25.01255189679135 25.49302568280839 25.457712684369856 112-113 23.94748195382705 25.042550560851907 25.535663551991917 25.474303933329125 114-115 23.74207213881028 25.24858359711587 25.46256885554384 25.546775408530014 116-117 23.727161076374443 25.211146496291683 25.346951056102405 25.714741371231465 118-119 23.635332892915713 24.877238246094365 25.599553838752563 25.88787502223736 120-121 23.538833144596268 24.948442557512195 25.701385080170507 25.81133921772103 122-123 23.569898483416218 24.991295055690273 25.55827545107065 25.88053100982286 124-125 23.433920950413047 25.01756997321997 25.53567452746885 26.012834548898134 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 56.0 1 51.5 2 88.5 3 121.5 4 91.0 5 88.5 6 148.5 7 327.0 8 556.5 9 640.5 10 514.0 11 397.0 12 436.0 13 520.0 14 605.0 15 742.5 16 920.0 17 1093.5 18 1269.0 19 1385.0 20 1558.5 21 1782.0 22 1995.0 23 2297.5 24 2649.5 25 3186.0 26 4200.0 27 5277.5 28 6107.0 29 7298.0 30 9111.5 31 10940.0 32 13131.5 33 15435.5 34 17516.0 35 19885.5 36 22761.0 37 25645.5 38 27724.5 39 30191.0 40 32621.5 41 34460.0 42 36558.0 43 40393.5 44 44635.5 45 47594.0 46 51165.0 47 54885.0 48 59165.5 49 62918.5 50 63773.5 51 63899.5 52 63364.0 53 63583.5 54 64476.0 55 63684.0 56 60108.0 57 54709.5 58 51366.0 59 47677.0 60 42254.0 61 37157.0 62 32176.5 63 25495.0 64 19163.0 65 14973.0 66 11366.0 67 8522.0 68 6596.0 69 5211.5 70 4291.5 71 3478.0 72 2720.0 73 2373.5 74 1872.0 75 1355.0 76 908.0 77 469.0 78 244.0 79 146.0 80 81.5 81 43.5 82 22.5 83 9.0 84 5.5 85 3.0 86 2.5 87 3.0 88 2.0 89 1.5 90 1.0 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12474501907671588 2 0.0015782184806753725 3 0.02485694107063711 4 0.05701314261439783 5 0.029591596512663233 6 0.02117443128239458 7 0.015584907496669301 8 0.006049837509255593 9 0.0040770644084113785 10-11 0.007397899128165808 12-13 0.005655282889086751 14-15 0.0032221960647122183 16-17 0.011573602191619396 18-19 0.005951198854213384 20-21 0.012658627397083715 22-23 0.010094022365986235 24-25 0.014401243636162771 26-27 0.009995383710944024 28-29 0.014467002739524247 30-31 0.022588252004666267 32-33 0.013513495740782875 34-35 0.022095058729455212 36-37 0.01614385987524183 38-39 0.022489613349624054 40-41 0.011014649813046869 42-43 0.009995383710944024 44-45 0.01837966938953194 46-47 0.018017994321043833 48-49 0.021996420074413003 50-51 0.016078100771880356 52-53 0.023048565728196583 54-55 0.017985114769363097 56-57 0.022062179177774473 58-59 0.017459041942471304 60-61 0.020911394868948684 62-63 0.0228841679697929 64-65 0.019201658181550365 66-67 0.013118941120614033 68-69 0.025021338829040795 70-71 0.015354750634904142 72-73 0.018412548941212675 74-75 0.020220924283653206 76-77 0.01473003915297014 78-79 0.030314946649639442 80-81 0.022193697384497422 82-83 0.010061142814305498 84-85 0.014401243636162771 86-87 0.013118941120614033 88-89 0.02114155173071384 90-91 0.01377653215422877 92-93 0.00940355178069076 94-95 0.007332140024804334 96-97 0.00299203920294706 98-99 0.0023015686176515843 100-101 0.012987422913891085 102-103 0.0033537142714351663 104-105 0.00299203920294706 106-107 3.2879551680736925E-4 108-109 0.02114155173071384 110-111 0.0010192661021028446 112-113 0.010389938331112868 114-115 0.0024002072726937956 116-117 0.008844599402118233 118-119 0.01154072263993866 120-121 0.004471619028580221 122-123 9.535069987413708E-4 124-125 0.02318008393491953 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1520702.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.51870584760593 #Duplication Level Percentage of deduplicated Percentage of total 1 78.37346299001955 43.51193238001126 2 10.942825059469108 12.150629712369527 3 3.8863511208737753 6.472955541009143 4 1.9077575945054444 4.236649308715362 5 1.1165460470379 3.099459577540218 6 0.7293940674234586 2.429700880576311 7 0.5273741359776962 2.0495390668886735 8 0.39108274841080987 1.7369926456874425 9 0.31081716289046285 1.5530549975012726 >10 1.7084975370560394 16.910566123607417 >50 0.07938498852819019 2.970952636986569 >100 0.02603111518153686 2.3746686922335987 >500 1.1885815650131215E-4 0.04592800315756002 >1k 3.5657446950393645E-4 0.4569704337156654 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2868 0.18859710844070698 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2596 0.1707106323263861 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 6.575910336147384E-5 0.0 0.0 0.0 6 0.0 6.575910336147384E-5 0.0 0.0 0.0 7 0.0 6.575910336147384E-5 0.0 0.0 0.0 8 0.0 6.575910336147384E-5 0.0 0.0 0.0 9 0.0 6.575910336147384E-5 0.0 0.0 6.575910336147384E-5 10-11 0.0 6.575910336147384E-5 0.0 0.0 6.575910336147384E-5 12-13 0.0 6.575910336147384E-5 0.0 0.0 1.6439775840368463E-4 14-15 0.0 6.575910336147384E-5 0.0 0.0 2.630364134458954E-4 16-17 0.0 6.575910336147384E-5 0.0 0.0 3.6167506848810613E-4 18-19 0.0 6.575910336147384E-5 0.0 0.0 3.9455462016884307E-4 20-21 0.0 1.315182067229477E-4 0.0 0.0 4.6031372353031694E-4 22-23 0.0 1.315182067229477E-4 0.0 0.0 5.589523785725276E-4 24-25 0.0 1.315182067229477E-4 0.0 3.6167506848810613E-4 5.918319302532646E-4 26-27 0.0 1.315182067229477E-4 0.0 4.931932752110539E-4 5.918319302532646E-4 28-29 0.0 1.315182067229477E-4 0.0 0.0014467002739524245 5.918319302532646E-4 30-31 0.0 1.315182067229477E-4 0.0 0.005425126027321592 5.918319302532646E-4 32-33 0.0 1.315182067229477E-4 0.0 0.014006689015993928 5.918319302532646E-4 34-35 0.0 1.315182067229477E-4 0.0 0.027585943860138275 6.575910336147385E-4 36-37 0.0 1.315182067229477E-4 0.0 0.04573545638790506 6.575910336147385E-4 38-39 0.0 1.315182067229477E-4 0.0 0.07733270555309324 6.575910336147385E-4 40-41 0.0 1.315182067229477E-4 0.0 0.11248094629980102 6.575910336147385E-4 42-43 0.0 1.315182067229477E-4 0.0 0.14506458201541128 6.575910336147385E-4 44-45 0.0 1.315182067229477E-4 0.0 0.18583522609952507 6.575910336147385E-4 46-47 0.0 1.315182067229477E-4 0.0 0.22874304104288679 6.904705852954754E-4 48-49 0.0 1.315182067229477E-4 0.0 0.2758923181530635 7.891092403376861E-4 50-51 0.0 1.315182067229477E-4 0.0 0.3261322731212295 7.891092403376861E-4 52-53 0.0 1.315182067229477E-4 0.0 0.3774901328465406 8.5486834369916E-4 54-55 0.0 1.315182067229477E-4 0.0 0.43121532029286475 9.206274470606339E-4 56-57 0.0 1.315182067229477E-4 0.0 0.4887545357341544 9.535069987413709E-4 58-59 0.0 1.9727731008442153E-4 0.0 0.5556973029561347 9.863865504221078E-4 60-61 0.0 1.9727731008442153E-4 0.0 0.6277692802403101 0.0010521456537835815 62-63 0.0 1.9727731008442153E-4 0.0 0.7047074311732344 0.0011836638605065293 64-65 0.0 1.9727731008442153E-4 0.0 0.7861829602381005 0.0011836638605065293 66-67 0.0 1.9727731008442153E-4 0.0 0.8744974360525599 0.0011836638605065293 68-69 0.0 1.9727731008442153E-4 0.0 0.974845827782169 0.0011836638605065293 70-71 0.0 1.9727731008442153E-4 0.0 1.0839073007071733 0.0011836638605065293 72-73 0.0 1.9727731008442153E-4 0.0 1.2041478212036283 0.0016439775840368463 74-75 0.0 1.9727731008442153E-4 0.0 1.320804470566883 0.00170973668739832 76-77 0.0 1.9727731008442153E-4 0.0 1.454887282320928 0.001742616239079057 78-79 0.0 1.9727731008442153E-4 0.0 1.6058044245355105 0.0017754957907597938 80-81 0.0 1.9727731008442153E-4 0.0 1.7687554826652427 0.0018083753424405309 82-83 0.0 1.9727731008442153E-4 0.0 1.945351554742481 0.0019070139974827415 84-85 0.0 1.9727731008442153E-4 0.0 2.1234600861970327 0.0019070139974827415 86-87 0.0 1.9727731008442153E-4 0.0 2.321362107763388 0.0019727731008442155 88-89 0.0 1.9727731008442153E-4 0.0 2.5360655802386 0.0020385322042056893 90-91 0.0 1.9727731008442153E-4 0.0 2.7758561506462147 0.0020385322042056893 92-93 0.0 1.9727731008442153E-4 0.0 3.0294232532080576 0.0022358095142901106 94-95 0.0 1.9727731008442153E-4 0.0 3.296602490165726 0.002334448169332322 96-97 0.0 1.9727731008442153E-4 0.0 3.5726263265255125 0.002400207272693795 98-99 0.0 1.9727731008442153E-4 0.0 3.8744606109546775 0.0024330868243745323 100-101 0.0 1.9727731008442153E-4 0.0 4.215520200538961 0.00256460503109748 102-103 0.0 1.9727731008442153E-4 0.0 4.582719033709432 0.002663243686139691 104-105 0.0 1.9727731008442153E-4 0.0 4.963398483069003 0.0026961232378204278 106-107 0.0 1.9727731008442153E-4 0.0 5.361109540199198 0.0027290027895011644 108-109 3.287955168073692E-5 1.9727731008442153E-4 0.0 5.781310210679015 0.0028605209962241124 110-111 6.575910336147384E-5 1.9727731008442153E-4 0.0 6.237119435629071 0.003024918754627797 112-113 6.575910336147384E-5 1.9727731008442153E-4 0.0 6.7167005764443 0.003024918754627797 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2460 0.0 83.15331 1 GTATCAA 4985 0.0 59.93673 1 TCAACGC 6695 0.0 44.378174 4 CAACGCA 6795 0.0 43.788197 5 TATCAAC 7005 0.0 42.502056 2 ATCAACG 6995 0.0 42.474895 3 AACGCAG 7125 0.0 41.996857 6 GTACATG 8510 0.0 35.600384 1 ACGCAGA 8395 0.0 35.28808 7 CGTATAC 85 1.7037525E-4 35.024387 3 TACATGG 8845 0.0 33.86244 2 CGCAGAG 8805 0.0 33.64491 8 ACATGGG 9165 0.0 32.09327 3 GCAGAGT 9380 0.0 30.820417 9 CATGGGG 7540 0.0 30.244534 4 TACCGTA 150 5.305119E-6 27.760426 7 AGAGTAC 8755 0.0 27.517183 10-11 CAGAGTA 9195 0.0 26.52389 10-11 GAGTACT 4855 0.0 25.668465 12-13 ATGGGGG 4160 0.0 25.605673 5 >>END_MODULE