FastQCFastQC Report
Mon 15 Aug 2016
SRR2921507_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921507_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1582001
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18600.067.178671
GTATCAA35650.060.5859761
TCAACGC43300.048.2299464
CAACGCA43900.047.162695
AACGCAG46900.044.778756
TATCAAC47900.044.0909272
ATCAACG47800.043.9342463
ACGCAGA56000.037.1823167
GTACATG79700.035.536421
CGCAGAG59700.034.578938
TACATGG82950.033.6366582
ACATGGG85150.032.208663
GCAGAGT64750.031.421659
CATGGGG73250.030.0532674
AGAGTAC58250.026.45168310-11
CAGAGTA62350.026.28661310-11
ATGGGGG41900.024.9909345
GTATAGA4850.022.0828841
GTGTACG3651.1277734E-1021.1921751
CCTATAC3401.0713848E-920.997073
Produced by FastQC (version 0.11.5)