FastQCFastQC Report
Mon 15 Aug 2016
SRR2921507_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921507_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1582001
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25600.16182037811606947No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22860.1445005407708339No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23650.078.3740161
GTATCAA44100.053.3828051
TCAACGC58000.039.926014
CAACGCA59650.038.9066355
ATCAACG61400.038.0059853
TATCAAC62600.037.3748972
AACGCAG62650.037.225316
GTACATG78600.034.5768281
TACATGG82000.032.3804442
ACGCAGA74800.031.017657
ACATGGG84700.030.5731473
CGCAGAG78350.029.685378
CATGGGG70450.029.4902694
GCAGAGT84350.026.657019
GTGGTAT9150.026.0544411
TAACCCG1150.001004271725.8726925
AGAGTAC76550.025.64408310-11
CAGAGTA80900.025.0740410-11
GAGTACT42500.023.72536712-13
ATGGGGA28350.023.5090145
Produced by FastQC (version 0.11.5)