FastQCFastQC Report
Mon 15 Aug 2016
SRR2921506_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921506_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1701751
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20150.068.8100051
GTATCAA36050.055.6282421
TCAACGC46350.042.357744
CAACGCA46850.041.905685
ATCAACG49700.039.618853
AACGCAG49750.038.981166
TATCAAC52950.037.5241432
GTACATG87900.036.0158351
ACATGGG91350.033.9932143
TACATGG93200.033.63762
ACGCAGA58850.032.9525227
CATGGGG76300.032.670674
CGCAGAG65050.029.718588
ATGGGGG41200.029.1690795
GCAGAGT68100.027.0773759
AGAGTAC62900.024.82393610-11
CAGAGTA65600.024.57295810-11
GTATAGA5400.023.1417521
ATGGGGA30550.022.9794355
GTGGTAT9450.020.1506421
Produced by FastQC (version 0.11.5)