##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921506_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1701751
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.116420674940105	31.0	30.0	34.0	22.0	34.0
2	29.118519689425774	31.0	30.0	34.0	22.0	34.0
3	29.27782795485356	31.0	30.0	34.0	22.0	34.0
4	32.63917400371735	37.0	33.0	37.0	23.0	37.0
5	32.65004809751838	37.0	33.0	37.0	24.0	37.0
6	32.64078411001375	37.0	33.0	37.0	23.0	37.0
7	32.5008552955162	37.0	33.0	37.0	22.0	37.0
8	32.40260884230419	37.0	33.0	37.0	19.0	37.0
9	33.81036062267629	39.0	34.0	39.0	19.0	39.0
10-11	33.677795693964626	39.0	34.0	39.0	18.0	39.0
12-13	33.512108704504946	38.0	34.0	39.0	17.0	39.0
14-15	34.567770490512416	39.0	34.0	41.0	17.0	41.0
16-17	34.474282371510284	39.0	34.0	41.0	17.0	41.0
18-19	34.472837095438756	39.0	34.0	41.0	17.5	41.0
20-21	34.4913395085415	39.0	34.0	41.0	18.0	41.0
22-23	34.44196683298556	39.0	34.0	41.0	18.0	41.0
24-25	34.38335690709158	39.0	34.0	41.0	18.0	41.0
26-27	34.28594400708447	39.0	34.0	41.0	17.0	41.0
28-29	34.23650316644445	39.0	34.0	41.0	17.0	41.0
30-31	34.12581394105248	39.0	33.0	41.0	16.0	41.0
32-33	34.014060517666806	39.0	33.0	41.0	16.0	41.0
34-35	33.84220987676811	39.0	33.0	41.0	15.0	41.0
36-37	33.69004219771283	39.0	33.0	41.0	10.5	41.0
38-39	33.550248244308364	39.0	33.0	41.0	9.0	41.0
40-41	33.39473871324301	38.0	33.0	41.0	8.5	41.0
42-43	33.193504513880114	38.0	32.5	41.0	7.5	41.0
44-45	32.98767681053221	38.0	32.0	40.0	4.5	41.0
46-47	32.7355720666537	38.0	32.0	40.0	2.0	41.0
48-49	32.59416918221291	38.0	32.0	40.0	2.0	41.0
50-51	32.39739803296722	38.0	31.0	40.0	2.0	41.0
52-53	32.19544163629109	37.0	31.0	40.0	2.0	41.0
54-55	31.986183348797795	37.0	31.0	40.0	2.0	41.0
56-57	31.751817392791306	36.5	31.0	40.0	2.0	41.0
58-59	31.500748346849804	36.0	30.5	40.0	2.0	41.0
60-61	31.193363776486688	35.5	30.0	39.5	2.0	41.0
62-63	30.88008439542565	35.0	30.0	39.0	2.0	41.0
64-65	30.583423485574563	35.0	30.0	39.0	2.0	40.5
66-67	30.55842188428272	35.0	30.0	38.5	2.0	40.0
68-69	30.321525593344738	35.0	30.0	37.5	2.0	40.0
70-71	30.029847198561953	35.0	30.0	37.0	2.0	40.0
72-73	29.71861717724861	35.0	29.0	36.5	2.0	39.0
74-75	29.426562993058326	35.0	29.0	36.0	2.0	39.0
76-77	29.15967377130967	35.0	29.0	36.0	2.0	38.5
78-79	28.897651007697363	34.5	29.0	35.0	2.0	37.0
80-81	28.66058195352904	34.0	29.0	35.0	2.0	37.0
82-83	28.46387779410736	34.0	29.0	35.0	2.0	36.0
84-85	28.26121682901905	34.0	29.0	35.0	2.0	36.0
86-87	28.112551424973454	34.0	28.5	35.0	2.0	36.0
88-89	27.98707272685604	34.0	28.0	35.0	2.0	35.0
90-91	27.877452400498075	34.0	28.0	35.0	2.0	35.0
92-93	27.776592462704592	34.0	27.5	35.0	2.0	35.0
94-95	27.708591621218382	34.0	27.0	35.0	2.0	35.0
96-97	27.603713763059343	34.0	27.0	35.0	2.0	35.0
98-99	27.542489471138843	34.0	27.0	35.0	2.0	35.0
100-101	27.482068469476438	34.0	27.0	35.0	2.0	35.0
102-103	26.500458939057474	32.5	25.0	34.5	2.0	35.0
104-105	27.31840674693301	34.0	26.5	35.0	2.0	35.0
106-107	27.648665697860615	34.0	28.0	35.0	2.0	35.0
108-109	27.693257121635305	34.0	28.0	35.0	2.0	35.0
110-111	27.63329652810546	34.0	28.0	35.0	2.0	35.0
112-113	27.557648856971436	34.0	27.5	35.0	2.0	35.0
114-115	27.434242142358077	34.0	27.0	35.0	2.0	35.0
116-117	27.36510952542411	34.0	27.0	35.0	2.0	35.0
118-119	27.19736612465631	34.0	27.0	35.0	2.0	35.0
120-121	27.012292338890944	34.0	27.0	35.0	2.0	35.0
122-123	26.87588254685909	34.0	27.0	35.0	2.0	35.0
124-125	26.01345819688074	33.0	23.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	90536.0
3	15311.0
4	9888.0
5	8525.0
6	8715.0
7	9920.0
8	10586.0
9	11051.0
10	11520.0
11	11686.0
12	11685.0
13	11611.0
14	12235.0
15	10549.0
16	8422.0
17	7203.0
18	6749.0
19	6237.0
20	6168.0
21	6382.0
22	7617.0
23	10618.0
24	15024.0
25	20321.0
26	23965.0
27	25445.0
28	29779.0
29	34765.0
30	39628.0
31	47032.0
32	58553.0
33	75224.0
34	104319.0
35	159041.0
36	287848.0
37	388401.0
38	99117.0
39	75.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.787319692956316	17.079863572715954	12.164354668613612	24.96846206571412
2	16.82769906084356	19.486467518635596	37.94255498984902	25.743278430671822
3	19.0547368371555	24.24704814659814	28.89307260783493	27.80514240841143
4	13.412287925625622	15.10482703407121	35.57729667517227	35.905588365130896
5	14.438145578266482	37.21787362713586	33.35251600646137	14.991464788136291
6	33.597143280430245	34.58975221226452	17.28570487932782	14.527399627977417
7	29.866714949406	31.18498657626194	20.6947188598376	18.253579614494463
8	28.067702396788995	33.1979348100198	19.466349548041297	19.268013245149902
9	27.984975708966083	14.412151101105367	18.742038918556702	38.86083427137185
10-11	26.45820560103789	24.75044807950391	26.911516863014352	21.87982945644385
12-13	26.657069243118336	22.46217413974421	26.96277402770242	23.917982589435034
14-15	24.20085217745536	23.648615086721122	24.227040013308475	27.923492722515043
16-17	24.373006476856027	26.597698946702614	24.735999633185862	24.2932949432555
18-19	24.478669231173992	25.76639055000804	26.199158510010662	23.55578170880731
20-21	25.599781737188525	24.78758771377937	25.747397251252718	23.865233297779387
22-23	25.411497984658656	24.368888456419917	25.67116555255277	24.548448006368663
24-25	24.889005445870144	24.83964186560817	25.66409303755954	24.60725965096215
26-27	24.48520597346264	24.960153986982363	25.763423741109975	24.791216298445022
28-29	24.55158680815526	25.018276932791156	25.850064773978936	24.580071485074647
30-31	24.913062317048723	24.90198015087032	25.64133994853407	24.543617583546894
32-33	24.549717333082466	24.980582267391437	25.59180721616449	24.877893183361603
34-35	24.826286012585005	25.058757066132415	25.736858874874198	24.378098046408383
36-37	24.941772428748944	24.834797731395618	25.575448322166988	24.647981517688454
38-39	24.903386080833766	25.075225824470625	25.518334227566385	24.503053867129218
40-41	24.90006176049451	24.888156477164934	25.685722272962575	24.52605948937799
42-43	24.733413611075505	25.026311735563233	25.52694027735803	24.713334376003225
44-45	24.67313046264747	25.015331426337127	25.550211523705645	24.761326587309753
46-47	24.818826465316025	25.01051804649814	25.472253672643113	24.698401815542717
48-49	24.90247221292173	25.177498955715578	25.180203365755876	24.739825465606813
50-51	24.85599328110729	25.347828221407156	25.122620673882363	24.673557823603197
52-53	25.00723225656989	25.146791288835157	25.2735321753099	24.57244427928505
54-55	24.83206463204135	25.09618143251544	25.424879714418747	24.64687422102446
56-57	24.766694140927672	25.224605023204187	25.099060192147377	24.90964064372076
58-59	24.84879180418656	25.063287567182414	25.211497466733608	24.876423161897414
60-61	24.87083760249666	25.193412938166055	25.41244085628982	24.52330860304746
62-63	24.69829973204407	25.370217739176237	25.283711124932832	24.647771403846853
64-65	24.73629259397084	24.975764726492667	25.4257559965033	24.86218668303319
66-67	24.673109587756706	25.21836047341097	25.323142932974203	24.785387005858116
68-69	24.71805461505182	25.249498006132082	25.265192662189868	24.767254716626226
70-71	24.599455524666745	24.994774835844595	25.51696789382819	24.88880174566047
72-73	24.821406843234797	25.279844440408112	25.257977690036466	24.640771026320632
74-75	24.648369206748207	25.407683616350614	25.235380706541864	24.708566470359315
76-77	24.66855787848827	25.050229190889734	25.313866313347855	24.96734661727414
78-79	24.586605188078806	25.17178431431908	25.38451794142742	24.8570925561747
80-81	24.666160006912204	25.38341858971081	25.149250090663884	24.801171312713105
82-83	24.811189567437502	25.23647588339773	25.24011985127909	24.712214697885678
84-85	24.549821444944154	25.321522525678308	25.14963830552545	24.97901772385209
86-87	24.38477800522963	25.397967273968984	25.361557345783826	24.855697375017556
88-89	24.579843354681945	25.184124813127383	25.260886955402427	24.97514487678824
90-91	24.5224648114954	25.168258352678443	25.340953836208165	24.968322999617996
92-93	24.583195826330638	25.347465379826424	25.219484685956534	24.8498541078864
94-95	24.539607601598696	25.085975079201685	25.339396965225898	25.03502035397372
96-97	24.51297861736939	25.130409128521457	25.33231113890247	25.024301115206683
98-99	24.401076063464824	25.16513401574132	25.2059492028697	25.227840717924156
100-101	24.368317185463273	25.224352155602546	25.380913817036777	25.026416841897404
102-103	24.426021851086695	24.99817089779388	25.51628429860468	25.059522952514747
104-105	24.31672834708716	25.229432065101616	25.376701108778406	25.07713847903282
106-107	24.30661685088027	25.194838394983556	25.35906593931309	25.139478814823086
108-109	24.285346548153303	25.08989000449009	25.400993462331417	25.22376998502519
110-111	24.295315068283845	25.32132104954788	25.386346286862082	24.997017595306197
112-113	24.237869737637016	25.32428074085044	25.46350166783027	24.974347853682275
114-115	24.036562890804976	25.53643125123412	25.445135682786248	24.98187017517466
116-117	24.072589649863072	25.509543846452203	25.344936002162644	25.072930501522077
118-119	24.208452907719206	25.124492839673724	25.450760092549395	25.21629416005768
120-121	23.88312437694178	25.233102277816812	25.712324530146656	25.17144881509475
122-123	23.963097208249902	25.31222805410395	25.547403852568696	25.177270885077448
124-125	23.93153226742435	25.366704325584603	25.55031726046798	25.151446146523064
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	109.0
1	128.0
2	149.5
3	158.0
4	141.0
5	115.0
6	130.0
7	174.5
8	231.0
9	279.0
10	291.0
11	310.0
12	369.5
13	423.0
14	492.0
15	566.0
16	620.5
17	689.5
18	749.5
19	869.5
20	1009.5
21	1110.5
22	1311.5
23	1526.5
24	1831.5
25	2441.0
26	3350.5
27	4485.0
28	5495.0
29	6849.5
30	8917.5
31	10916.5
32	13245.5
33	15974.0
34	18725.5
35	21395.0
36	25146.5
37	28872.0
38	31110.0
39	33978.5
40	36776.0
41	38829.0
42	41188.0
43	45791.0
44	51053.5
45	54655.5
46	58375.5
47	62130.0
48	66695.0
49	71181.0
50	71276.0
51	70605.0
52	70383.0
53	70402.0
54	71552.0
55	70605.5
56	66501.0
57	60689.5
58	57351.5
59	53707.0
60	48041.5
61	43050.0
62	37479.5
63	29596.0
64	22528.5
65	18098.5
66	14180.0
67	10958.5
68	8696.0
69	7076.0
70	5991.5
71	4928.0
72	3958.0
73	3530.5
74	2957.5
75	2278.0
76	1593.0
77	926.0
78	565.0
79	352.5
80	196.5
81	97.0
82	57.5
83	35.5
84	29.0
85	24.5
86	15.0
87	9.5
88	11.0
89	9.5
90	8.5
91	8.0
92	7.5
93	8.5
94	7.0
95	5.0
96	3.5
97	4.5
98	5.5
99	6.5
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03672687719883814
2	0.025679432537427625
3	0.026267062572609037
4	0.03055676182943333
5	0.03308357098071339
6	0.04577637974063186
7	0.03878358232197307
8	0.03525780211088461
9	0.02973407978017936
10-11	0.03537532811792089
12-13	0.03525780211088461
14-15	0.03405316053876272
16-17	0.03625677317069301
18-19	0.049331541453479386
20-21	0.06164239069052993
22-23	0.05420887074548509
24-25	0.06490373738578677
26-27	0.048655766913020766
28-29	0.049155252442924964
30-31	0.04818566288487564
32-33	0.05794032146888704
34-35	0.039900079388817754
36-37	0.0517995876012413
38-39	0.06102537915358945
40-41	0.04850885940422541
42-43	0.05811661047944147
44-45	0.058527951504068455
46-47	0.06478621137875047
48-49	0.04850885940422541
50-51	0.05144700958013246
52-53	0.060937234648312244
54-55	0.04695163981099468
56-57	0.04495369769137788
58-59	0.0461289577617407
60-61	0.03514027610384833
62-63	0.04260317755065224
64-65	0.042191836526025255
66-67	0.035698524637270676
68-69	0.03143820688220545
70-71	0.04980164548162451
72-73	0.03161449589275987
74-75	0.039782553381781466
76-77	0.03226088893145942
78-79	0.033054189478954325
80-81	0.0242103574494741
82-83	0.018157768087105577
84-85	0.01851034610821442
86-87	0.01715879702729718
88-89	0.023005715877352208
90-91	0.011811363707146345
92-93	0.018951068634600478
94-95	0.014132502346112914
96-97	0.010753629643819807
98-99	0.010489196127988173
100-101	0.010871155650856088
102-103	0.005670629839500609
104-105	0.006081970864127596
106-107	0.008726306022443942
108-109	0.013339201798618012
110-111	0.006111352365886666
112-113	0.008168057489021602
114-115	0.007933005474949038
116-117	0.007697953460876474
118-119	0.23875408329420694
120-121	0.35154966854727865
122-123	0.02764799315528535
124-125	0.004319080758583365
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1701751.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.56503727726705
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.78314101450529	47.31067664889223
2	9.857031908538245	11.54554882333506
3	3.3956078432293078	5.965916997531143
4	1.6753883256162756	3.924767189744608
5	0.9831377997020548	2.8788750944120567
6	0.6448696427131904	2.266008879868552
7	0.4828731771952281	1.9795639935841638
8	0.3484592639642416	1.6326023826939877
9	0.25425747991110803	1.3401538910116217
>10	1.474679460433614	15.353524983282052
>50	0.07566410890036336	3.0191808183342337
>100	0.024181822619127477	2.4560176720254643
>500	5.058233371124277E-4	0.18650995008994115
>1k	2.0232933484497107E-4	0.14065267519489208
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.876300351814102E-5
2	0.0	0.0	0.0	0.0	5.876300351814102E-5
3	0.0	0.0	0.0	0.0	5.876300351814102E-5
4	0.0	0.0	0.0	0.0	5.876300351814102E-5
5	0.0	0.0	0.0	0.0	1.1752600703628204E-4
6	0.0	0.0	0.0	0.0	1.7628901055442305E-4
7	0.0	0.0	0.0	5.876300351814102E-5	2.3505201407256408E-4
8	0.0	0.0	0.0	5.876300351814102E-5	2.3505201407256408E-4
9	0.0	0.0	0.0	5.876300351814102E-5	2.3505201407256408E-4
10-11	0.0	0.0	0.0	5.876300351814102E-5	2.3505201407256408E-4
12-13	0.0	0.0	0.0	5.876300351814102E-5	2.938150175907051E-4
14-15	0.0	0.0	0.0	8.814450527721153E-5	4.407225263860576E-4
16-17	0.0	0.0	0.0	1.1752600703628204E-4	7.051560422176922E-4
18-19	0.0	0.0	0.0	1.4690750879535255E-4	7.639190457358333E-4
20-21	0.0	0.0	0.0	1.7628901055442305E-4	8.226820492539743E-4
22-23	0.0	0.0	0.0	1.7628901055442305E-4	9.695895580493269E-4
24-25	0.0	0.0	0.0	2.3505201407256408E-4	0.0010577340633265383
26-27	0.0	0.0	0.0	3.8195952286791663E-4	0.0011164970668446795
28-29	0.0	0.0	0.0	0.0012340230738809614	0.0011164970668446795
30-31	0.0	0.0	0.0	0.0038195952286791666	0.00114587856860375
32-33	0.0	0.0	0.0	0.010753629643819807	0.0012927860773991025
34-35	0.0	0.0	0.0	0.01968560617857724	0.0012927860773991025
36-37	0.0	0.0	0.0	0.032760374461363616	0.0012927860773991025
38-39	0.0	0.0	0.0	0.05329804419095391	0.0013515490809172435
40-41	0.0	0.0	0.0	0.075422315015534	0.0013515490809172435
42-43	0.0	0.0	0.0	0.09619503675919686	0.0013515490809172435
44-45	0.0	0.0	0.0	0.12187446929662447	0.0014103120844353844
46-47	0.0	0.0	0.0	0.15196112709791268	0.0015278380914716665
48-49	0.0	0.0	0.0	0.18548542060501214	0.001615982596748878
50-51	0.0	0.0	0.0	0.22253549432320005	0.0016453640985079486
52-53	0.0	0.0	0.0	0.2592036085185201	0.0018216531090623717
54-55	0.0	0.0	0.0	0.2981340983492885	0.0019979421196167947
56-57	0.0	0.0	0.0	0.343352229556498	0.002027323621375865
58-59	0.0	0.0	0.0	0.39194923346600063	0.002174231130171218
60-61	0.0	0.0	0.0	0.44427768809890517	0.002350520140725641
62-63	0.0	0.0	0.0	0.49969120041651216	0.002350520140725641
64-65	0.0	0.0	0.0	0.5571907993590132	0.002350520140725641
66-67	0.0	0.0	0.0	0.6225940222747042	0.0023799016424847115
68-69	0.0	0.0	0.0	0.698780256335974	0.0024092831442437818
70-71	0.0	0.0	0.0	0.7831345478862655	0.002526809151280064
72-73	0.0	0.0	0.0	0.8757744229326148	0.0028500056706298395
74-75	0.0	0.0	0.0	0.9682967719719278	0.002996913179425192
76-77	0.0	0.0	0.0	1.0691047045072986	0.002996913179425192
78-79	0.0	0.0	0.0	1.1837219428694326	0.002996913179425192
80-81	0.0	0.0	0.0	1.312941787605825	0.003055676182943333
82-83	0.0	0.0	0.0	1.4469214356271864	0.0032025836917386856
84-85	0.0	0.0	0.0	1.5848382048842633	0.0032319651934977563
86-87	0.0	0.0	0.0	1.73383180030451	0.003261346695256827
88-89	0.0	0.0	0.0	1.9050378110546138	0.0033201096987749675
90-91	0.0	0.0	0.0	2.097545410580044	0.003349491200534038
92-93	0.0	0.0	0.0	2.298896842134954	0.00346701720757032
94-95	0.0	0.0	0.0	2.5112369553477567	0.00346701720757032
96-97	0.0	0.0	0.0	2.7308930624985677	0.0034963987093293908
98-99	0.0	0.0	0.0	2.973525504024972	0.0035257802110884614
100-101	0.0	0.0	0.0	3.257380192519352	0.0035257802110884614
102-103	0.0	0.0	0.0	3.5583642965392706	0.0036139247163656726
104-105	0.0	0.0	0.0	3.8701607932065265	0.003672687719883814
106-107	0.0	0.0	0.0	4.198058352837753	0.0038783582321973075
108-109	0.0	0.0	0.0	4.53262551336829	0.004084028744510801
110-111	0.0	0.0	0.0	4.90277367252906	0.004319080758583365
112-113	0.0	0.0	0.0	5.30074611385567	0.0044072252638605765
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2015	0.0	68.810005	1
GTATCAA	3605	0.0	55.628242	1
TCAACGC	4635	0.0	42.35774	4
CAACGCA	4685	0.0	41.90568	5
ATCAACG	4970	0.0	39.61885	3
AACGCAG	4975	0.0	38.98116	6
TATCAAC	5295	0.0	37.524143	2
GTACATG	8790	0.0	36.015835	1
ACATGGG	9135	0.0	33.993214	3
TACATGG	9320	0.0	33.6376	2
ACGCAGA	5885	0.0	32.952522	7
CATGGGG	7630	0.0	32.67067	4
CGCAGAG	6505	0.0	29.71858	8
ATGGGGG	4120	0.0	29.169079	5
GCAGAGT	6810	0.0	27.077375	9
AGAGTAC	6290	0.0	24.823936	10-11
CAGAGTA	6560	0.0	24.572958	10-11
GTATAGA	540	0.0	23.141752	1
ATGGGGA	3055	0.0	22.979435	5
GTGGTAT	945	0.0	20.150642	1
>>END_MODULE