FastQCFastQC Report
Mon 15 Aug 2016
SRR2921506_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921506_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1701751
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23820.13997347438021193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21110.12404870042679571No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24650.078.1226351
GTATCAA49300.058.2896421
TCAACGC64150.044.736974
CAACGCA65000.043.9416125
ATCAACG67950.042.1474953
AACGCAG68300.041.812376
TATCAAC69300.040.983982
GTACATG90050.037.407271
TACATGG94800.035.3613622
ACGCAGA81900.034.6502157
ACATGGG95300.034.4250763
CGCAGAG85500.033.0520948
CATGGGG78000.032.2895434
GCAGAGT90650.030.5171439
ATGGGGG41250.029.860325
GAGTACT47450.026.39291612-13
AGAGTAC85150.026.34036810-11
CAGAGTA89250.026.2635310-11
GTGGTAT9750.025.0709321
GTATAGG4050.025.0256371
Produced by FastQC (version 0.11.5)