##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921506_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1701751
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.244521672089512	31.0	31.0	34.0	28.0	34.0
2	31.298615954978136	33.0	31.0	34.0	28.0	34.0
3	31.379032537662678	33.0	31.0	34.0	28.0	34.0
4	34.9236127964667	37.0	35.0	37.0	32.0	37.0
5	34.58637250690612	37.0	35.0	37.0	31.0	37.0
6	34.56467426785705	37.0	35.0	37.0	31.0	37.0
7	34.40969624815851	37.0	35.0	37.0	30.0	37.0
8	34.3751829733022	37.0	35.0	37.0	30.0	37.0
9	35.95023126180035	39.0	37.0	39.0	30.0	39.0
10-11	35.81804417920131	39.0	36.0	39.0	30.0	39.0
12-13	35.73455576050786	39.0	35.0	39.0	30.0	39.0
14-15	36.90054391036057	40.0	37.0	41.0	30.0	41.0
16-17	36.87191134308134	40.0	37.0	41.0	30.0	41.0
18-19	36.882931756761124	40.0	37.0	41.0	30.0	41.0
20-21	36.86022602601673	40.0	37.0	41.0	30.0	41.0
22-23	36.79951267841182	40.0	37.0	41.0	30.0	41.0
24-25	36.73658014597905	40.0	36.5	41.0	30.0	41.0
26-27	36.608601082061945	40.0	36.0	41.0	30.0	41.0
28-29	36.41335130697735	40.0	36.0	41.0	29.0	41.0
30-31	36.36461062752424	40.0	36.0	41.0	29.0	41.0
32-33	36.24908520694273	40.0	35.5	41.0	29.0	41.0
34-35	36.12673769546779	40.0	35.0	41.0	28.5	41.0
36-37	35.99545291878776	40.0	35.0	41.0	28.0	41.0
38-39	35.82281426601189	40.0	35.0	41.0	27.0	41.0
40-41	35.622274351535566	39.0	35.0	41.0	27.0	41.0
42-43	35.45367800577171	39.0	35.0	41.0	26.0	41.0
44-45	35.23386265088136	39.0	35.0	41.0	25.5	41.0
46-47	34.95934746035113	39.0	34.5	41.0	24.0	41.0
48-49	34.71054372819525	39.0	34.0	41.0	23.5	41.0
50-51	34.48714118575514	39.0	34.0	41.0	23.0	41.0
52-53	34.285156582837324	38.0	34.0	40.5	22.5	41.0
54-55	34.06755953132979	38.0	33.5	40.0	22.0	41.0
56-57	33.80817757709559	37.5	33.0	40.0	21.5	41.0
58-59	33.66509524601425	37.0	33.0	40.0	21.5	41.0
60-61	33.54330862740789	37.0	33.0	40.0	21.5	41.0
62-63	33.28137283304079	36.0	33.0	40.0	21.0	41.0
64-65	32.985504048477125	36.0	33.0	39.0	20.0	41.0
66-67	32.68491365658078	35.0	33.0	39.0	20.0	41.0
68-69	32.37131137281541	35.0	32.0	39.0	19.0	40.5
70-71	32.07040336688505	35.0	32.0	37.5	18.0	40.0
72-73	31.763725421639244	35.0	32.0	37.0	18.0	39.0
74-75	31.469843120409507	35.0	32.0	36.5	17.0	39.0
76-77	31.182124470618792	35.0	32.0	36.0	14.5	39.0
78-79	30.910941436203064	35.0	31.0	35.5	13.0	37.0
80-81	30.689631444318234	35.0	31.0	35.0	11.5	37.0
82-83	30.503652120668654	35.0	31.0	35.0	10.5	36.5
84-85	30.316956182191166	35.0	31.0	35.0	8.0	36.0
86-87	30.17217589412317	35.0	31.0	35.0	7.0	36.0
88-89	30.070794434673463	35.0	31.0	35.0	7.0	36.0
90-91	29.974526237974885	35.0	31.0	35.0	7.0	35.0
92-93	30.03712411510262	35.0	31.0	35.0	7.0	35.0
94-95	30.0069266890397	35.0	31.0	35.0	6.0	35.0
96-97	29.988955199673747	35.0	31.0	35.0	2.0	35.0
98-99	29.950939355992737	35.0	31.0	35.0	2.0	35.0
100-101	29.903128894885327	35.0	31.0	35.0	2.0	35.0
102-103	29.006751869104235	33.5	29.5	35.0	2.0	35.0
104-105	29.71751448948759	34.5	31.0	35.0	2.0	35.0
106-107	29.822887425951272	35.0	31.0	35.0	2.0	35.0
108-109	29.833678370102323	35.0	31.0	35.0	2.0	35.0
110-111	29.799095754901863	35.0	31.0	35.0	2.0	35.0
112-113	29.751530923149154	35.0	31.0	35.0	2.0	35.0
114-115	29.686311628434478	35.0	31.0	35.0	2.0	35.0
116-117	29.608402169295037	35.0	31.0	35.0	2.0	35.0
118-119	29.512498597033293	35.0	31.0	35.0	2.0	35.0
120-121	29.38777970455137	35.0	31.0	35.0	2.0	35.0
122-123	29.2624987439408	35.0	31.0	35.0	2.0	35.0
124-125	28.340597713766584	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	26202.0
3	9864.0
4	5767.0
5	4840.0
6	5018.0
7	6218.0
8	7001.0
9	8020.0
10	9032.0
11	9679.0
12	10192.0
13	11854.0
14	14718.0
15	12012.0
16	6786.0
17	6108.0
18	5410.0
19	4967.0
20	4767.0
21	5118.0
22	6255.0
23	8271.0
24	11360.0
25	15742.0
26	19660.0
27	22520.0
28	26540.0
29	31689.0
30	36991.0
31	44167.0
32	55229.0
33	70983.0
34	97986.0
35	154309.0
36	301101.0
37	474993.0
38	150126.0
39	256.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.89635510246825	17.715470429111175	12.475569879264313	24.912604589156263
2	17.16588941019397	19.866463890482056	37.20024304935265	25.76740364997132
3	19.116212257641337	24.437993562963296	29.16420791299421	27.28158626640116
4	13.461363204748821	15.558375142065413	35.43201115239231	35.54825050079345
5	14.336826340266054	37.22891382139817	33.306628327447655	15.127631510888119
6	33.60103255413739	35.00645346335867	16.725460970064866	14.66705301243908
7	29.6256614720589	31.572992898064538	20.433873951812156	18.367471678064398
8	28.210649167025625	33.22841697552937	19.192406531689695	19.3685273257553
9	27.996262549986923	14.058453483144747	18.694478152665432	39.2508058142029
10-11	26.538527764049213	24.692018243544624	26.71679664888581	22.05265734352035
12-13	26.902126964389183	22.377582310125206	27.131665642185283	23.588625083300325
14-15	24.391480104436063	23.40685345514015	24.612228515887832	27.589437924535954
16-17	24.22542942239837	26.42308243828706	25.112248088256383	24.23924005105819
18-19	24.234957424177423	25.271997508330053	27.117331797587074	23.375713269905447
20-21	25.397658435271982	24.243360997252804	26.869577201317973	23.48940336615724
22-23	25.090884835719706	23.864894534803632	26.767596926194344	24.276623703282315
24-25	24.580249126529125	24.392860606096225	26.80925276733792	24.21763750003673
26-27	24.18522089207789	24.483849574359365	26.927797561311735	24.40313197225101
28-29	24.340918253763956	24.558868644411454	26.92300006934919	24.1772130324754
30-31	24.624582440811434	24.47085659473888	26.736248354669122	24.16831260978056
32-33	24.251725047853512	24.5893883624725	26.670523940860875	24.48836264881311
34-35	24.504046328388245	24.627384553716634	26.823228140746046	24.045340977149074
36-37	24.692464882724963	24.407630150342666	26.57913868421811	24.320766282714267
38-39	24.650626571129827	24.66602562708541	26.447555341092027	24.235792460692736
40-41	24.682030818415825	24.52494152493565	26.546298146428615	24.246729510219915
42-43	24.568819103834848	24.62435487416006	26.346433898328993	24.460392123676105
44-45	24.575872975385042	24.640552511937504	26.221513160272718	24.562061352404736
46-47	24.749129579259417	24.704932658468294	25.971832691145806	24.574105071126482
48-49	24.942789946312036	24.955485332567694	25.484753634568598	24.61697108655168
50-51	24.825257023365868	25.229900223273717	25.338422179501723	24.6064205738587
52-53	25.00338693755898	25.021342849476852	25.475295339485754	24.499974873478415
54-55	24.79068099937113	25.036556624565815	25.592810922320115	24.57995145374294
56-57	24.67561320733924	25.120393322567708	25.379179823704266	24.824813646388787
58-59	24.758136607194807	24.871685012492087	25.576513817113018	24.79366456320009
60-61	24.792483503591978	25.11765096556957	25.698015376468398	24.391850154370058
62-63	24.636832746017877	25.144889268187605	25.67137194950821	24.54690603628631
64-65	24.676762707770212	24.850848595235032	25.72107232675016	24.7513163702446
66-67	24.707525139482588	25.051768777586876	25.590628345282685	24.650077737647855
68-69	24.706858047851956	25.0403576215732	25.5026371681572	24.750147162417644
70-71	24.5828936515087	24.86837461567327	25.75423247935883	24.794499253459197
72-73	24.892099734609573	25.161633671876814	25.394052327551325	24.552214265962288
74-75	24.66863168041001	25.30165801707975	25.352409443820783	24.677300858689456
76-77	24.720478606097064	24.950213764186174	25.391729593079614	24.937578036637145
78-79	24.612291009806757	25.079528542106043	25.464798186772754	24.84338226131445
80-81	24.67287176361543	25.227768710065906	25.255451843396926	24.84390768292174
82-83	24.795964512296784	25.138376831030264	25.290733123198205	24.774925533474747
84-85	24.538844494490583	25.196659968816505	25.2355071079686	25.028988428724315
86-87	24.42497712367789	25.24361740328326	25.36556568428266	24.965839788756192
88-89	24.490165959263823	25.12604975339593	25.291089192708526	25.092695094631722
90-91	24.448738563382136	25.080867349337545	25.378801235577875	25.091592851702444
92-93	24.540577643487488	25.224484542822562	25.27379048383585	24.9611473298541
94-95	24.44062589252448	25.02092106763264	25.406873628524114	25.131579411318768
96-97	24.462180214766512	25.019825663443534	25.40767117282425	25.110322948965702
98-99	24.38707637738501	24.996195028444735	25.255697026715897	25.36103156745436
100-101	24.349263393283486	25.05943836584531	25.39051836484622	25.20077987602498
102-103	24.457285202234587	24.878951894855703	25.503739056161205	25.1600238467485
104-105	24.348457420661493	25.077253106651742	25.308020777905067	25.266268694781697
106-107	24.401658297806076	24.98996618177224	25.30094109281943	25.307434427602253
108-109	24.409725569115533	24.93802206637781	25.26771881237466	25.384533552131995
110-111	24.403287223665192	25.06399332447965	25.30498195964131	25.227737492213855
112-113	24.276331564026496	25.04340012505818	25.385076938989474	25.295191371925853
114-115	24.069787531115796	25.198388912786445	25.410910685029393	25.320912871068362
116-117	24.09826291850964	25.245082843187237	25.249725481945575	25.40692875635755
118-119	24.142860543067293	24.85235007203618	25.376981509195453	25.62780787570108
120-121	23.927419212665058	25.014720660049715	25.49309803783298	25.564762089452252
122-123	23.92387192501781	25.013757997340953	25.453279368880338	25.609090708760903
124-125	23.883274874768382	25.13016888084298	25.37059595408933	25.615960290299306
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	29.0
1	26.5
2	45.0
3	68.5
4	56.0
5	59.5
6	111.0
7	242.5
8	394.0
9	453.5
10	384.5
11	329.5
12	385.5
13	457.0
14	549.5
15	669.0
16	831.0
17	969.5
18	1072.5
19	1241.0
20	1461.0
21	1684.5
22	1960.0
23	2271.5
24	2581.0
25	3054.0
26	3936.5
27	5062.0
28	6041.5
29	7338.5
30	9425.0
31	11384.0
32	13441.5
33	16078.0
34	18728.0
35	21423.0
36	25006.0
37	28383.0
38	30684.0
39	33490.5
40	36045.0
41	38198.0
42	40726.0
43	45352.0
44	50494.5
45	54178.5
46	58527.0
47	62354.0
48	66910.0
49	71588.5
50	71743.0
51	71029.0
52	70743.5
53	71003.5
54	71968.5
55	70460.5
56	66245.5
57	60642.5
58	57311.0
59	53993.0
60	48113.0
61	42427.5
62	36961.0
63	29155.0
64	21988.0
65	17546.0
66	13653.0
67	10295.0
68	7991.0
69	6632.5
70	5756.5
71	4669.5
72	3701.0
73	3400.5
74	2811.0
75	2097.0
76	1414.0
77	804.0
78	483.0
79	288.0
80	139.5
81	46.5
82	28.5
83	19.0
84	7.5
85	4.5
86	1.5
87	3.0
88	2.5
89	1.5
90	1.5
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11781982205387276
2	0.0022917571372075
3	0.020390762220794934
4	0.05582485334223397
5	0.026267062572609037
6	0.019920658192649807
7	0.014279409854908267
8	0.004583514274415
9	0.003290728197015897
10-11	0.006346404379959231
12-13	0.004524751270896858
14-15	0.002350520140725641
16-17	0.009578369573456987
18-19	0.004759803284969422
20-21	0.01154693019131471
22-23	0.00863816151716673
24-25	0.01210517872473705
26-27	0.008197438990780672
28-29	0.012751571763436602
30-31	0.020302617715517723
32-33	0.011987652717700768
34-35	0.01950931716802282
36-37	0.013985594837317563
38-39	0.020449525224313075
40-41	0.010048473601602114
42-43	0.008520635510130447
44-45	0.01677683750442926
46-47	0.016159825967488783
48-49	0.020302617715517723
50-51	0.014808276886571539
52-53	0.02124282577180798
54-55	0.01651240398859763
56-57	0.02047890672607215
58-59	0.01610106296397064
60-61	0.018804161125805127
62-63	0.021477877785880544
64-65	0.01715879702729718
66-67	0.011429404184278428
68-69	0.02347581990549734
70-71	0.013897450332040352
72-73	0.017364467539610674
74-75	0.018862924129323267
76-77	0.013309820296858942
78-79	0.027912426671116985
80-81	0.02080210324542192
82-83	0.0086675430189258
84-85	0.012340230738809615
86-87	0.012340230738809615
88-89	0.019950039694408877
90-91	0.011693837700110064
92-93	0.007668571959117403
94-95	0.0068752714116225
96-97	0.0024974276495209934
98-99	0.0017628901055442307
100-101	0.011664456198350994
102-103	0.0030262946811842623
104-105	0.0023211386389665706
106-107	3.525780211088461E-4
108-109	0.019920658192649807
110-111	6.463930386995511E-4
112-113	0.008403109503094166
114-115	0.002526809151280064
116-117	0.0077860979661536845
118-119	0.01031290711743375
120-121	0.0035845432146066024
122-123	7.933005474949038E-4
124-125	0.0237108719195699
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1701751.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.340153016483065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.85272650192012	43.63721950383148
2	10.719162310085089	11.864001648972538
3	3.7422300884417607	6.212867571517619
4	1.8506350965608038	4.096577176853952
5	1.1018788307878735	3.048907155071219
6	0.7421585652263994	2.464270113727348
7	0.5162110352736952	1.99970383765904
8	0.3857229256516941	1.7076772582024218
9	0.29905311537392676	1.4894680648364163
>10	1.6696383674547894	16.58319737188908
>50	0.08908319114950164	3.370201433779922
>100	0.030435923165176692	2.9281709901165103
>500	6.384293454919119E-4	0.20132654808804298
>1k	4.256195636612746E-4	0.39641132545443264
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2382	0.13997347438021193	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2111	0.12404870042679571	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.876300351814102E-5
2	0.0	0.0	0.0	0.0	5.876300351814102E-5
3	0.0	0.0	0.0	0.0	5.876300351814102E-5
4	0.0	0.0	0.0	0.0	5.876300351814102E-5
5	0.0	0.0	0.0	0.0	5.876300351814102E-5
6	0.0	0.0	0.0	0.0	1.1752600703628204E-4
7	0.0	0.0	0.0	0.0	1.1752600703628204E-4
8	0.0	0.0	0.0	0.0	1.7628901055442305E-4
9	0.0	0.0	0.0	0.0	2.3505201407256408E-4
10-11	0.0	0.0	0.0	0.0	2.3505201407256408E-4
12-13	0.0	0.0	0.0	0.0	4.7010402814512815E-4
14-15	0.0	0.0	0.0	0.0	6.170115369404807E-4
16-17	0.0	0.0	0.0	0.0	8.226820492539743E-4
18-19	0.0	0.0	0.0	0.0	9.989710598083974E-4
20-21	0.0	0.0	0.0	0.0	0.0010283525615674678
22-23	0.0	0.0	0.0	0.0	0.001087115565085609
24-25	0.0	0.0	0.0	1.1752600703628204E-4	0.0011752600703628204
26-27	0.0	5.876300351814102E-5	0.0	2.644335158316346E-4	0.0012046415721218909
28-29	0.0	5.876300351814102E-5	0.0	0.0011164970668446795	0.0012340230738809614
30-31	0.0	5.876300351814102E-5	0.0	0.0038489767304382364	0.0012634045756400318
32-33	0.0	5.876300351814102E-5	0.0	0.011076826163169583	0.0012927860773991025
34-35	0.0	5.876300351814102E-5	0.0	0.020273236213758653	0.0013515490809172435
36-37	0.0	5.876300351814102E-5	0.0	0.033847490026449226	0.0013515490809172435
38-39	0.0	5.876300351814102E-5	0.0	0.055266604808811626	0.001439693586194455
40-41	0.0	1.1752600703628204E-4	0.0	0.077919742665055	0.0014690750879535256
42-43	0.0	1.1752600703628204E-4	0.0	0.09998525048611695	0.001498456589712596
44-45	0.0	1.1752600703628204E-4	0.0	0.12651674657455764	0.001557219593230737
46-47	0.0	1.1752600703628204E-4	0.0	0.15816062396907657	0.001615982596748878
48-49	0.0	1.1752600703628204E-4	0.0	0.19300708505533418	0.0017628901055442307
50-51	0.0	1.1752600703628204E-4	0.0	0.23164375986851188	0.0017628901055442307
52-53	0.0	1.1752600703628204E-4	0.0	0.26960466014123097	0.0018216531090623717
54-55	0.0	1.1752600703628204E-4	0.0	0.31026865857578456	0.001909797614339583
56-57	0.0	1.1752600703628204E-4	0.0	0.3565445238463206	0.0019391791160986538
58-59	0.0	1.1752600703628204E-4	0.0	0.40684565485784935	0.0019979421196167947
60-61	0.0	1.1752600703628204E-4	0.0	0.4602024620523214	0.0020567051231349357
62-63	0.0	1.1752600703628204E-4	0.0	0.5173788644754727	0.002232994133689359
64-65	0.0	1.1752600703628204E-4	0.0	0.576700116527036	0.0022623756354484296
66-67	0.0	1.1752600703628204E-4	0.0	0.6445420040887297	0.00232113863896657
68-69	0.0	1.1752600703628204E-4	0.0	0.722549891259062	0.002350520140725641
70-71	0.0	1.1752600703628204E-4	0.0	0.8094309919606335	0.0024092831442437818
72-73	0.0	1.1752600703628204E-4	0.0	0.9052146876952034	0.002820624168870769
74-75	0.0	1.1752600703628204E-4	0.0	1.0007633314157007	0.002996913179425192
76-77	0.0	1.1752600703628204E-4	0.0	1.104685703137533	0.003055676182943333
78-79	0.0	1.1752600703628204E-4	0.0	1.2230931552265871	0.003055676182943333
80-81	0.0	1.1752600703628204E-4	0.0	1.3558681616758268	0.003114439186461474
82-83	0.0	1.1752600703628204E-4	0.0	1.4937555494311447	0.003261346695256827
84-85	0.0	1.1752600703628204E-4	0.0	1.6359913994468052	0.0032907281970158972
86-87	0.0	1.1752600703628204E-4	0.0	1.7897447981520211	0.003349491200534038
88-89	0.0	1.1752600703628204E-4	0.0	1.9670621612680117	0.00346701720757032
90-91	0.0	1.1752600703628204E-4	0.0	2.166033691180437	0.00346701720757032
92-93	0.0	1.1752600703628204E-4	0.0	2.375553180224369	0.0035845432146066024
94-95	0.0	1.1752600703628204E-4	0.0	2.5944747498312033	0.0037020692216428843
96-97	0.0	1.1752600703628204E-4	0.0	2.821094272898914	0.004113410246269871
98-99	0.0	1.1752600703628204E-4	0.0	3.072629309458317	0.0044072252638605765
100-101	0.0	1.1752600703628204E-4	0.0	3.3658273155120813	0.0044072252638605765
102-103	0.0	1.1752600703628204E-4	0.0	3.67565525156148	0.004524751270896858
104-105	0.0	1.1752600703628204E-4	0.0	3.997705892342652	0.0046716587796922105
106-107	0.0	1.1752600703628204E-4	0.0	4.337003474656399	0.004730421783210353
108-109	0.0	1.1752600703628204E-4	0.0	4.684880455483793	0.004847947790246634
110-111	0.0	1.1752600703628204E-4	0.0	5.068602868457254	0.005024236800801057
112-113	0.0	1.1752600703628204E-4	0.0	5.481912453702099	0.005053618302560128
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2465	0.0	78.122635	1
GTATCAA	4930	0.0	58.289642	1
TCAACGC	6415	0.0	44.73697	4
CAACGCA	6500	0.0	43.941612	5
ATCAACG	6795	0.0	42.147495	3
AACGCAG	6830	0.0	41.81237	6
TATCAAC	6930	0.0	40.98398	2
GTACATG	9005	0.0	37.40727	1
TACATGG	9480	0.0	35.361362	2
ACGCAGA	8190	0.0	34.650215	7
ACATGGG	9530	0.0	34.425076	3
CGCAGAG	8550	0.0	33.052094	8
CATGGGG	7800	0.0	32.289543	4
GCAGAGT	9065	0.0	30.517143	9
ATGGGGG	4125	0.0	29.86032	5
GAGTACT	4745	0.0	26.392916	12-13
AGAGTAC	8515	0.0	26.340368	10-11
CAGAGTA	8925	0.0	26.26353	10-11
GTGGTAT	975	0.0	25.070932	1
GTATAGG	405	0.0	25.025637	1
>>END_MODULE