FastQCFastQC Report
Mon 15 Aug 2016
SRR2921505_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921505_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1640169
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17300.066.737551
GTATCAA33000.060.0544131
TCAACGC43250.045.391054
CAACGCA43800.044.4136055
AACGCAG46500.041.9626856
ATCAACG48750.040.6360973
TATCAAC50550.038.4830062
GTACATG84050.037.598581
TACATGG88750.035.392232
ACATGGG89400.035.068363
ACGCAGA55150.035.0574387
CGCAGAG59050.032.5365878
CATGGGG74750.032.2319224
GCAGAGT64600.029.0967129
TGTACGC2252.055458E-1029.083892
ATGGGGG41500.028.956475
AGAGTAC58400.026.22771310-11
ATAGGAC4450.025.4001313
CAGAGTA62650.024.02124210-11
TTAGCGC1350.002558293322.0332534
Produced by FastQC (version 0.11.5)