##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921505_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1640169 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.95479063437975 31.0 30.0 34.0 22.0 34.0 2 29.051655652557756 31.0 30.0 34.0 22.0 34.0 3 29.137708979989256 31.0 30.0 34.0 22.0 34.0 4 32.496795757022596 35.0 33.0 37.0 22.0 37.0 5 32.48335384951185 35.0 33.0 37.0 22.0 37.0 6 32.57252758709621 37.0 33.0 37.0 23.0 37.0 7 32.4458705170016 37.0 33.0 37.0 22.0 37.0 8 32.41563643746468 37.0 33.0 37.0 21.0 37.0 9 33.8309588828956 39.0 34.0 39.0 19.0 39.0 10-11 33.686068630732564 39.0 34.0 39.0 18.0 39.0 12-13 33.51912699240139 38.5 34.0 39.0 17.0 39.0 14-15 34.589997738037965 39.5 34.0 41.0 17.0 41.0 16-17 34.4984004697077 39.0 34.0 41.0 17.0 41.0 18-19 34.49762951256852 39.5 34.0 41.0 17.5 41.0 20-21 34.50687337707273 39.5 34.0 41.0 18.0 41.0 22-23 34.46591113476721 39.0 34.0 41.0 18.0 41.0 24-25 34.41247578755604 39.0 34.0 41.0 18.0 41.0 26-27 34.315158986665395 39.0 34.0 41.0 17.0 41.0 28-29 34.2570619247163 39.0 34.0 41.0 17.0 41.0 30-31 34.14644801846639 39.0 33.0 41.0 16.0 41.0 32-33 34.0380652847359 39.0 33.0 41.0 15.5 41.0 34-35 33.87136691401923 39.0 33.0 41.0 15.0 41.0 36-37 33.72565936802854 39.0 33.0 41.0 10.0 41.0 38-39 33.59564807041225 39.0 33.0 41.0 9.0 41.0 40-41 33.44135634803487 38.5 33.0 41.0 8.5 41.0 42-43 33.2404365037993 38.0 32.5 41.0 7.5 41.0 44-45 33.04562731035644 38.0 32.0 40.5 4.5 41.0 46-47 32.79374198634409 38.0 32.0 40.0 2.0 41.0 48-49 32.656668306741565 38.0 32.0 40.0 2.0 41.0 50-51 32.463576009545356 38.0 32.0 40.0 2.0 41.0 52-53 32.25928059852369 37.5 31.0 40.0 2.0 41.0 54-55 32.04886478161701 37.0 31.0 40.0 2.0 41.0 56-57 31.802765446731406 37.0 31.0 40.0 2.0 41.0 58-59 31.55034359264198 36.0 31.0 40.0 2.0 41.0 60-61 31.24015238673576 35.5 30.0 39.5 2.0 41.0 62-63 30.926785898282432 35.0 30.0 39.0 2.0 41.0 64-65 30.62188134271529 35.0 30.0 39.0 2.0 40.5 66-67 30.583142346916688 35.0 30.0 38.5 2.0 40.0 68-69 30.342978680855445 35.0 30.0 37.5 2.0 40.0 70-71 30.04852762123903 35.0 30.0 37.0 2.0 39.5 72-73 29.729379716358498 35.0 29.5 36.5 2.0 39.0 74-75 29.442316614934192 35.0 29.0 36.0 2.0 39.0 76-77 29.16732940325052 35.0 29.0 36.0 2.0 38.0 78-79 28.902182031241903 34.5 29.0 35.0 2.0 37.0 80-81 28.670919277220822 34.0 29.0 35.0 2.0 37.0 82-83 28.484444285924194 34.0 29.0 35.0 2.0 36.0 84-85 28.292158003230156 34.0 29.0 35.0 2.0 36.0 86-87 28.14208261465739 34.0 29.0 35.0 2.0 36.0 88-89 28.020818891224014 34.0 28.5 35.0 2.0 35.0 90-91 27.912459325837766 34.0 28.0 35.0 2.0 35.0 92-93 27.816454584862903 34.0 28.0 35.0 2.0 35.0 94-95 27.754743566059354 34.0 27.5 35.0 2.0 35.0 96-97 27.651395069654406 34.0 27.0 35.0 2.0 35.0 98-99 27.59149849802063 34.0 27.0 35.0 2.0 35.0 100-101 27.526893874960443 34.0 27.0 35.0 2.0 35.0 102-103 26.565847482789884 32.5 25.0 34.5 2.0 35.0 104-105 27.370005468948627 34.0 27.0 35.0 2.0 35.0 106-107 27.697886010526965 34.0 28.0 35.0 2.0 35.0 108-109 27.742921918412065 34.0 29.0 35.0 2.0 35.0 110-111 27.685432415805934 34.0 28.5 35.0 2.0 35.0 112-113 27.607899856661113 34.0 28.0 35.0 2.0 35.0 114-115 27.48209666199032 34.0 27.0 35.0 2.0 35.0 116-117 27.414821887256743 34.0 27.0 35.0 2.0 35.0 118-119 27.252976065271323 34.0 27.0 35.0 2.0 35.0 120-121 27.073534495530645 34.0 27.0 35.0 2.0 35.0 122-123 26.939918996152226 34.0 27.0 35.0 2.0 35.0 124-125 26.088114395528756 33.0 23.5 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 88959.0 3 14382.0 4 9384.0 5 7992.0 6 8486.0 7 9280.0 8 10112.0 9 10449.0 10 10679.0 11 11035.0 12 10903.0 13 11150.0 14 11908.0 15 10144.0 16 7920.0 17 6969.0 18 6454.0 19 5958.0 20 5754.0 21 6227.0 22 7319.0 23 10108.0 24 14302.0 25 19230.0 26 22658.0 27 24319.0 28 28413.0 29 33323.0 30 37292.0 31 45286.0 32 55827.0 33 71120.0 34 98622.0 35 152953.0 36 280008.0 37 380855.0 38 94318.0 39 71.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.28402672185231 16.88590271640449 11.852225936640203 24.977844625103003 2 17.13449689675087 19.150068944151275 37.763953033817096 25.95148112528076 3 19.47984863223182 23.84469252278597 28.482449694001698 28.193009150980508 4 13.452394892587224 14.916421493288837 35.14560371199813 36.485579902125814 5 14.619989326827781 37.1125714721354 33.14202942746054 15.125409773576276 6 34.28152295905919 34.16026204069683 16.98878885473088 14.5694261455131 7 30.28612241337982 30.899708028395718 20.317429917971044 18.496739640253413 8 28.3368351716714 33.20261158869613 19.096800163453008 19.363753076179467 9 27.915124954183163 14.334495551268814 18.48316837238463 39.267211122163395 10-11 26.72384060540986 24.570013780661515 26.669925558812075 22.036220055116548 12-13 26.85497561768481 22.2368056315814 26.76663146521689 24.1415872855169 14-15 24.334722276435457 23.380626927280534 23.990251375931926 28.294399420352086 16-17 24.616702021119966 26.40931918179345 24.492036404311822 24.481942392774762 18-19 24.68782462670407 25.65928767103233 25.910937224553383 23.741950477710212 20-21 25.762854966735716 24.61075248875914 25.595122803796073 24.031269740709075 22-23 25.52125716614226 24.216284208073414 25.477061634618554 24.785396991165772 24-25 24.996629323845415 24.740732470806286 25.50583020211245 24.75680800323585 26-27 24.68822454054865 24.722535853034127 25.601515431550915 24.987724174866308 28-29 24.72325139655263 24.887000928391835 25.585429738061748 24.80431793699379 30-31 25.15200590456323 24.716361373908907 25.433509616264388 24.698123105263477 32-33 24.71557496254435 24.787497132873618 25.44730221708173 25.049625687500303 34-35 25.034788753054592 24.83497642781795 25.549323788370938 24.58091103075652 36-37 25.07228492337798 24.681427837686492 25.415714559342568 24.83057267959296 38-39 25.100971787684873 24.82034632232702 25.349782866046176 24.728899023941928 40-41 25.101752568861595 24.689130461966574 25.54764917635699 24.661467792814843 42-43 24.93009749900488 24.795157534173455 25.386739952325687 24.88800501449598 44-45 24.86402168820521 24.814852321086946 25.388322092296878 24.932803898410963 46-47 24.947525852725867 24.84921213282205 25.267022564569665 24.93623944988242 48-49 25.072587354916788 25.017475863179545 25.007045243271335 24.902891538632332 50-51 24.993831451150736 25.187107565157106 24.986145546033235 24.832915437658922 52-53 25.111151916974034 25.06530632663046 25.05127507476217 24.772266681633333 54-55 24.997643990314895 24.946284504104945 25.24221151995716 24.813859985623004 56-57 24.93958398649316 25.030284251091512 24.98639801008377 25.043733752331555 58-59 25.027852608843236 24.92546968205786 25.067835932351024 24.97884177674788 60-61 25.07947728116188 25.02964864714039 25.205299156298157 24.68557491539958 62-63 24.934446517568457 25.127889522482754 25.127554054556846 24.810109905391943 64-65 24.899673349417313 24.87064045288879 25.193539936548483 25.036146261145415 66-67 24.84570790779374 25.10552975295578 25.13602527468841 24.91273706456207 68-69 24.895893685924605 25.07281953075924 25.115785493422454 24.915501289893697 70-71 24.807748731083677 24.832727518938434 25.304274575131863 25.055249174846022 72-73 25.0387885766422 25.121702208631312 25.046168164336706 24.793341050389778 74-75 24.778167321734845 25.232323528048383 25.060749339442424 24.92875981077435 76-77 24.85506917128932 24.93886695516117 25.093990436440695 25.11207343710882 78-79 24.85338352284509 24.991309131449842 25.1737868767275 24.981520468977564 80-81 24.82369414135205 25.184203336577703 24.969385838449924 25.022716683620327 82-83 24.949874136517973 25.132327401163018 25.01991009054365 24.89788837177536 84-85 24.76562166513199 25.190868788379568 24.961246928110157 25.082262618378287 86-87 24.61567591036483 25.28733599529725 25.066040934403 25.030947159934925 88-89 24.735509306761028 25.045729592435674 25.097504120160814 25.121256980642485 90-91 24.726634135633372 25.01090719509016 25.142372814178287 25.12008585509818 92-93 24.77264086021899 25.23274069182062 25.023271782403572 24.97134666555682 94-95 24.769876339497912 24.91198576039821 25.125043180104562 25.193094719999316 96-97 24.680983912534334 25.012660033957246 25.170129210090103 25.136226843418324 98-99 24.673413041039225 25.033323333284553 25.01756106127347 25.275702564402753 100-101 24.645719872888733 25.11520716252419 25.117036452567575 25.122036512019502 102-103 24.602455023151506 24.920582464364237 25.313127642424142 25.163834870060107 104-105 24.5345217447771 25.095994934333216 25.12059768526832 25.24888563562137 106-107 24.48185797904109 25.060845350220134 25.2279471237672 25.229349546971573 108-109 24.539329624676895 25.05105347916731 25.1690755859793 25.24054131017649 110-111 24.519579897260797 25.154428913311893 25.18107403624834 25.14491715317897 112-113 24.506010674736388 25.20306663158623 25.191512038879527 25.099410654797854 114-115 24.288678953640773 25.351281790967782 25.262015103996603 25.09802415139484 116-117 24.276808135906567 25.355471838008683 25.166147014231676 25.201573011853075 118-119 24.42975818813457 24.980441867117158 25.2896742837445 25.300125661003776 120-121 24.117923663879253 25.06321469315978 25.481035897991294 25.33782574496967 122-123 24.275859923963463 25.170819972459014 25.28013621688343 25.273183886694095 124-125 24.171015462996667 25.21683010092622 25.320847577813428 25.29130685826369 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 92.0 1 111.5 2 138.5 3 142.0 4 119.0 5 116.0 6 133.0 7 153.5 8 197.0 9 239.0 10 263.5 11 281.5 12 319.0 13 397.5 14 452.5 15 501.0 16 577.5 17 656.5 18 712.0 19 775.5 20 883.0 21 1025.0 22 1224.0 23 1516.0 24 1834.0 25 2255.0 26 3107.5 27 4099.5 28 4866.5 29 6034.0 30 7745.5 31 9498.0 32 11529.0 33 13955.5 34 16595.5 35 18886.0 36 21861.0 37 25040.5 38 27270.0 39 30129.0 40 33057.0 41 35521.5 42 38388.5 43 42968.5 44 47603.0 45 51358.5 46 55132.0 47 58708.0 48 63411.5 49 68285.5 50 69592.5 51 69460.0 52 69693.5 53 70433.0 54 71521.0 55 70860.0 56 67725.5 57 62520.5 58 58624.0 59 54717.0 60 48859.0 61 43288.0 62 37598.0 63 29753.5 64 22561.0 65 17840.5 66 13721.5 67 10416.5 68 8256.0 69 6643.5 70 5653.5 71 4696.0 72 3740.5 73 3375.0 74 2775.0 75 2000.0 76 1388.0 77 826.5 78 506.0 79 339.0 80 210.5 81 110.5 82 66.5 83 40.0 84 36.0 85 28.5 86 14.5 87 10.0 88 10.0 89 12.5 90 11.5 91 9.0 92 9.0 93 11.0 94 9.5 95 8.0 96 7.0 97 6.5 98 9.5 99 8.5 100 19.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03731322808808117 2 0.026582626546410765 3 0.027680074431354333 4 0.03127726472089157 5 0.03316731385607215 6 0.04444663934021433 7 0.038166798665259495 8 0.03377700712548524 9 0.029753031547358838 10-11 0.03368555313507328 12-13 0.03405136909672113 14-15 0.03371603779854393 16-17 0.0355451176067832 18-19 0.04792189097586895 20-21 0.05782940660383168 22-23 0.052616529150349756 24-25 0.06292034540343099 26-27 0.046824443090925386 28-29 0.04725122837951455 30-31 0.0462757191484536 32-33 0.05468948626635426 34-35 0.038441160636495386 36-37 0.05017775607269739 38-39 0.05935363977736441 40-41 0.0472817130429852 42-43 0.05618323477641633 44-45 0.05712825934400663 46-47 0.06270695275913642 48-49 0.04664153511010146 50-51 0.049049823524283165 52-53 0.059201216460011134 54-55 0.04539166390780462 56-57 0.042861436839740294 58-59 0.04484293996533284 60-61 0.03350264515424935 62-63 0.04072751039679448 64-65 0.03959957784838026 66-67 0.035606086933724516 68-69 0.03042369414371324 70-71 0.047434136360338476 72-73 0.031155326067008948 74-75 0.03932521587714437 76-77 0.030941933422714365 78-79 0.03152114202865681 80-81 0.024692577411230182 82-83 0.018107890101568803 84-85 0.01822982875545142 86-87 0.01688850356274262 88-89 0.022345258323989786 90-91 0.011980472743967237 92-93 0.018748068034452548 94-95 0.014358276494678293 96-97 0.010090423608786654 98-99 0.01079157086861171 100-101 0.011492718128436765 102-103 0.006279840674954837 104-105 0.006615171973132037 106-107 0.009175883704667018 108-109 0.013535190580970618 110-111 0.005731116732483056 112-113 0.007834558511958219 114-115 0.00850522110831262 116-117 0.007956497165840837 118-119 0.24515766363100389 120-121 0.35712783255871805 122-123 0.02627777991170422 124-125 0.003658159616478546 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1640169.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.400220739214674 #Duplication Level Percentage of deduplicated Percentage of total 1 80.90916395399844 48.060221986928184 2 9.786265987493392 11.626127197395522 3 3.3926512556733313 6.045727004345091 4 1.6670473299310307 3.9609191752248663 5 1.0312324299521465 3.062771698629711 6 0.6456993409669822 2.3012810030762507 7 0.465155366216986 1.9341232001923443 8 0.32571299594702496 1.547793908550737 9 0.2626513518019696 1.404139344704111 >10 1.4233331055751157 14.950974590087979 >50 0.07039514341598753 2.8256867727101778 >100 0.0200710565753712 1.996038084226058 >500 4.137883014918034E-4 0.15122301394581447 >1k 2.068941507459017E-4 0.13297301998323546 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.0969326941309094E-5 2 0.0 0.0 0.0 0.0 1.2193865388261819E-4 3 0.0 0.0 0.0 0.0 1.2193865388261819E-4 4 0.0 0.0 0.0 0.0 1.2193865388261819E-4 5 6.0969326941309094E-5 0.0 0.0 6.0969326941309094E-5 1.2193865388261819E-4 6 6.0969326941309094E-5 0.0 0.0 6.0969326941309094E-5 1.2193865388261819E-4 7 6.0969326941309094E-5 0.0 0.0 6.0969326941309094E-5 1.2193865388261819E-4 8 6.0969326941309094E-5 0.0 0.0 6.0969326941309094E-5 1.2193865388261819E-4 9 6.0969326941309094E-5 0.0 0.0 1.2193865388261819E-4 1.2193865388261819E-4 10-11 6.0969326941309094E-5 0.0 0.0 1.2193865388261819E-4 1.2193865388261819E-4 12-13 6.0969326941309094E-5 0.0 0.0 1.2193865388261819E-4 2.1339264429458184E-4 14-15 6.0969326941309094E-5 0.0 0.0 1.5242331735327273E-4 3.658159616478546E-4 16-17 6.0969326941309094E-5 0.0 0.0 1.829079808239273E-4 4.267852885891637E-4 18-19 6.0969326941309094E-5 0.0 0.0 1.829079808239273E-4 4.572699520598182E-4 20-21 6.0969326941309094E-5 0.0 0.0 1.829079808239273E-4 5.487239424717819E-4 22-23 6.0969326941309094E-5 0.0 0.0 2.1339264429458184E-4 5.487239424717819E-4 24-25 6.0969326941309094E-5 0.0 0.0 3.0484663470654546E-4 5.487239424717819E-4 26-27 6.0969326941309094E-5 0.0 0.0 3.9630062511850913E-4 5.487239424717819E-4 28-29 6.0969326941309094E-5 0.0 0.0 0.0010364785580022546 5.487239424717819E-4 30-31 6.0969326941309094E-5 0.0 0.0 0.0034752516356546187 5.487239424717819E-4 32-33 6.0969326941309094E-5 0.0 0.0 0.00911491437772571 6.706625963544E-4 34-35 6.0969326941309094E-5 0.0 0.0 0.016736080245389348 7.621165867663638E-4 36-37 9.145399041196364E-5 0.0 0.0 0.03008836284553604 7.926012502370183E-4 38-39 1.2193865388261819E-4 0.0 0.0 0.04984242477452019 7.926012502370183E-4 40-41 1.2193865388261819E-4 0.0 0.0 0.07118168920397837 7.926012502370183E-4 42-43 1.2193865388261819E-4 0.0 0.0 0.09008218055578418 8.535705771783274E-4 44-45 1.2193865388261819E-4 0.0 0.0 0.11212259224506743 0.0010059938945316002 46-47 1.2193865388261819E-4 0.0 0.0 0.1388271574453608 0.0010974478849435638 48-49 1.2193865388261819E-4 0.0 0.0 0.16854970432924898 0.0013413251927088 50-51 1.2193865388261819E-4 0.0 0.0 0.20144265621408525 0.0013413251927088 52-53 1.2193865388261819E-4 0.0 0.0 0.23457948540668674 0.001402294519650109 54-55 1.2193865388261819E-4 0.0 0.0 0.2680821305609361 0.0014632638465914183 56-57 1.2193865388261819E-4 0.0 0.0 0.3074378311015511 0.0014632638465914183 58-59 1.2193865388261819E-4 0.0 0.0 0.3509089612107045 0.001493748510062073 60-61 1.2193865388261819E-4 0.0 0.0 0.4004160546870475 0.0015242331735327275 62-63 1.2193865388261819E-4 0.0 0.0 0.4507767187405688 0.0017071411543566548 64-65 1.2193865388261819E-4 0.0 0.0 0.502204346015563 0.0017071411543566548 66-67 1.2193865388261819E-4 0.0 0.0 0.560003267955924 0.0017071411543566548 68-69 1.2193865388261819E-4 0.0 0.0 0.6318251350927861 0.0017071411543566548 70-71 1.2193865388261819E-4 0.0 0.0 0.7087989103561889 0.0017681104812979638 72-73 1.2193865388261819E-4 0.0 0.0 0.7912599250443095 0.0021339264429458184 74-75 1.2193865388261819E-4 0.0 0.0 0.8704285960775993 0.0021339264429458184 76-77 1.2193865388261819E-4 0.0 0.0 0.9596267213927345 0.0021339264429458184 78-79 1.2193865388261819E-4 0.0 0.0 1.0647073563760807 0.0021339264429458184 80-81 1.2193865388261819E-4 0.0 0.0 1.1803356849202735 0.002225380433357782 82-83 1.2193865388261819E-4 0.0 0.0 1.3042863265919549 0.0022558650968284365 84-85 1.2193865388261819E-4 0.0 0.0 1.4285722995618135 0.0022558650968284365 86-87 1.2193865388261819E-4 0.0 0.0 1.5609367083513956 0.0022558650968284365 88-89 1.2193865388261819E-4 0.0 0.0 1.712841786425667 0.002377803750711055 90-91 1.2193865388261819E-4 0.0 0.0 1.8794709569562649 0.002377803750711055 92-93 1.2193865388261819E-4 0.0 0.0 2.0643604409057845 0.002377803750711055 94-95 1.2193865388261819E-4 0.0 0.0 2.249219440191834 0.0026826503854176 96-97 1.2193865388261819E-4 0.0 0.0 2.4440774090962574 0.002774104375829564 98-99 1.2193865388261819E-4 0.0 0.0 2.659695433824197 0.0028045890393002186 100-101 1.2193865388261819E-4 0.0 0.0 2.905157944089908 0.0028045890393002186 102-103 1.2193865388261819E-4 0.0 0.0 3.180282031912565 0.0029265276931828366 104-105 1.2193865388261819E-4 3.0484663470654547E-5 0.0 3.460923843823411 0.0030789510105361092 106-107 1.2193865388261819E-4 6.0969326941309094E-5 0.0 3.7510463860736305 0.0031399203374774184 108-109 1.2193865388261819E-4 6.0969326941309094E-5 0.0 4.050466750682399 0.003292343654830691 110-111 1.2193865388261819E-4 6.0969326941309094E-5 0.0 4.382780067176004 0.0034752516356546187 112-113 1.2193865388261819E-4 6.0969326941309094E-5 0.0 4.737773973291777 0.0034752516356546187 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1730 0.0 66.73755 1 GTATCAA 3300 0.0 60.054413 1 TCAACGC 4325 0.0 45.39105 4 CAACGCA 4380 0.0 44.413605 5 AACGCAG 4650 0.0 41.962685 6 ATCAACG 4875 0.0 40.636097 3 TATCAAC 5055 0.0 38.483006 2 GTACATG 8405 0.0 37.59858 1 TACATGG 8875 0.0 35.39223 2 ACATGGG 8940 0.0 35.06836 3 ACGCAGA 5515 0.0 35.057438 7 CGCAGAG 5905 0.0 32.536587 8 CATGGGG 7475 0.0 32.231922 4 GCAGAGT 6460 0.0 29.096712 9 TGTACGC 225 2.055458E-10 29.08389 2 ATGGGGG 4150 0.0 28.95647 5 AGAGTAC 5840 0.0 26.227713 10-11 ATAGGAC 445 0.0 25.400131 3 CAGAGTA 6265 0.0 24.021242 10-11 TTAGCGC 135 0.0025582933 22.033253 4 >>END_MODULE