FastQCFastQC Report
Mon 15 Aug 2016
SRR2921505_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921505_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1640169
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23080.1407172065805414No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22000.13413251927088002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21500.078.196771
GTATCAA41500.059.7617571
TCAACGC58850.041.69064
CAACGCA60850.040.6819845
ATCAACG62000.039.669713
AACGCAG63500.038.9759146
TATCAAC63800.038.6450232
GTACATG80050.033.663191
ACGCAGA74250.033.250787
TACATGG82800.032.0788232
CGCAGAG78450.031.4677458
ACATGGG85200.030.8247573
GTATACG1004.388192E-429.809051
GCGCGAC2502.1827873E-1128.5529989
GCAGAGT85100.028.3796449
AGAGTAC76550.027.66528310-11
CATGGGG71300.027.4785464
GTATAGG4500.026.4969331
CCTATAC3400.026.2732033
CAGAGTA82450.024.78371610-11
Produced by FastQC (version 0.11.5)