##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921505_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1640169
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.310000981606162	31.0	31.0	34.0	28.0	34.0
2	31.365904367171918	33.0	31.0	34.0	28.0	34.0
3	31.447870920618545	33.0	31.0	34.0	28.0	34.0
4	34.989324880545844	37.0	35.0	37.0	32.0	37.0
5	34.660435601453266	37.0	35.0	37.0	32.0	37.0
6	34.64614865907111	37.0	35.0	37.0	31.0	37.0
7	34.48566946454908	37.0	35.0	37.0	30.0	37.0
8	34.45226254123813	37.0	35.0	37.0	30.0	37.0
9	36.03947702950123	39.0	37.0	39.0	30.0	39.0
10-11	35.91252608725077	39.0	37.0	39.0	30.0	39.0
12-13	35.831016194062926	39.0	36.0	39.0	30.0	39.0
14-15	37.01180640531555	40.0	37.0	41.0	30.0	41.0
16-17	36.98076935974281	40.0	37.0	41.0	30.0	41.0
18-19	36.98926695968525	40.0	37.0	41.0	30.0	41.0
20-21	36.964209480852276	40.0	37.0	41.0	30.0	41.0
22-23	36.91024949258278	40.0	37.0	41.0	30.0	41.0
24-25	36.84484220833341	40.0	37.0	41.0	30.0	41.0
26-27	36.723458375325954	40.0	36.0	41.0	30.0	41.0
28-29	36.52720695245429	40.0	36.0	41.0	29.5	41.0
30-31	36.48269141777463	40.0	36.0	41.0	30.0	41.0
32-33	36.365492214521794	40.0	35.5	41.0	29.5	41.0
34-35	36.250727516493725	40.0	35.0	41.0	29.0	41.0
36-37	36.12426859671168	40.0	35.0	41.0	28.5	41.0
38-39	35.95461260394508	40.0	35.0	41.0	28.0	41.0
40-41	35.751188444605404	39.5	35.0	41.0	27.0	41.0
42-43	35.587553782567525	39.0	35.0	41.0	27.0	41.0
44-45	35.3796486825443	39.0	35.0	41.0	26.0	41.0
46-47	35.11785431867082	39.0	35.0	41.0	24.5	41.0
48-49	34.86429142362769	39.0	34.0	41.0	24.0	41.0
50-51	34.64266548142295	39.0	34.0	41.0	23.5	41.0
52-53	34.439390392087645	38.0	34.0	41.0	23.0	41.0
54-55	34.22053916395201	38.0	34.0	40.0	23.0	41.0
56-57	33.95775282912919	37.5	33.0	40.0	22.0	41.0
58-59	33.80701988636537	37.0	33.0	40.0	22.0	41.0
60-61	33.68258941609066	37.0	33.0	40.0	22.0	41.0
62-63	33.40721931703379	36.0	33.0	40.0	22.0	41.0
64-65	33.10170110519099	36.0	33.0	39.0	21.5	41.0
66-67	32.800465378872545	35.0	33.0	39.0	20.5	41.0
68-69	32.478801269869145	35.0	33.0	38.5	20.0	40.5
70-71	32.17329738581817	35.0	32.0	37.5	20.0	40.0
72-73	31.85677939285525	35.0	32.0	37.0	19.5	39.0
74-75	31.56433087078222	35.0	32.0	36.5	18.0	39.0
76-77	31.27432234117338	35.0	32.0	36.0	18.0	38.5
78-79	31.00269514909744	35.0	32.0	35.5	17.0	37.0
80-81	30.79044049729022	35.0	31.0	35.0	15.5	37.0
82-83	30.615108869878654	35.0	31.0	35.0	15.0	36.5
84-85	30.42891006963307	35.0	31.0	35.0	12.5	36.0
86-87	30.291170909826974	35.0	31.0	35.0	11.0	36.0
88-89	30.191841206607368	35.0	31.0	35.0	10.0	35.5
90-91	30.0925864956599	35.0	31.0	35.0	8.0	35.0
92-93	30.15813400936123	35.0	31.0	35.0	9.0	35.0
94-95	30.12822428664363	35.0	31.0	35.0	7.0	35.0
96-97	30.11291580318857	35.0	31.0	35.0	7.0	35.0
98-99	30.07890894170052	35.0	31.0	35.0	7.0	35.0
100-101	30.031206235455006	35.0	31.0	35.0	6.0	35.0
102-103	29.13591831085699	33.5	29.5	35.0	2.0	35.0
104-105	29.84719288073363	34.5	31.0	35.0	2.0	35.0
106-107	29.957330616540126	35.0	31.0	35.0	2.0	35.0
108-109	29.963785134336767	35.0	31.0	35.0	2.0	35.0
110-111	29.929885883710764	35.0	31.0	35.0	2.0	35.0
112-113	29.887077185338825	35.0	31.0	35.0	2.0	35.0
114-115	29.820166702333722	35.0	31.0	35.0	2.0	35.0
116-117	29.746627024410287	35.0	31.0	35.0	2.0	35.0
118-119	29.649217245296065	35.0	31.0	35.0	2.0	35.0
120-121	29.52596653149767	35.0	31.0	35.0	2.0	35.0
122-123	29.402343599958296	35.0	31.0	35.0	2.0	35.0
124-125	28.473574369470462	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	23372.0
3	8902.0
4	5309.0
5	4460.0
6	4699.0
7	5569.0
8	6652.0
9	7402.0
10	8372.0
11	8939.0
12	9511.0
13	10975.0
14	13690.0
15	11587.0
16	6632.0
17	5614.0
18	5112.0
19	4732.0
20	4566.0
21	4863.0
22	6027.0
23	7809.0
24	10915.0
25	15002.0
26	18618.0
27	21595.0
28	25203.0
29	30620.0
30	35725.0
31	42604.0
32	53156.0
33	67873.0
34	94027.0
35	148285.0
36	293661.0
37	466854.0
38	140980.0
39	257.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.40051558128646	17.328378153173915	12.26266687258989	25.008439392949732
2	17.361280472934084	19.503409456730626	36.897674838306884	26.237635232028406
3	19.370251118997963	23.9600331735636	28.826790091837108	27.84292561560133
4	13.61104383627997	15.231699602187145	34.934203181648755	36.22305337988413
5	14.50335871298631	36.96871198007031	33.280012684744456	15.247916622198913
6	34.38580486692368	34.522277768088756	16.391444283322134	14.700473081665432
7	30.077307046417157	31.12081192521254	20.157211726670383	18.644669301699928
8	28.386847770880447	33.320427513906274	18.80961192912327	19.483112786090008
9	27.946381481673427	13.945326671158249	18.3980293944595	39.710262452708825
10-11	26.71049037349944	24.508278985595613	26.553178671947787	22.228051968957157
12-13	27.034191228700216	22.161985343567675	26.916362975649637	23.887460452082465
14-15	24.497050223031916	23.21614097470679	24.329197060710044	27.957611741551247
16-17	24.478990886970816	26.22836566384017	24.80246363675443	24.490179812434583
18-19	24.54983319477357	25.123309044980253	26.763009146163935	23.563848614082243
20-21	25.49388634905052	24.08670240902695	26.671676898006226	23.747734343916306
22-23	25.296044789178794	23.730624698558998	26.539643264580903	24.433687247681306
24-25	24.7248497302538	24.289231341474753	26.540678397590195	24.445240530681247
26-27	24.471632335433398	24.31117770891037	26.63534605364849	24.58184390200774
28-29	24.59015693704164	24.376919638746624	26.593288726472707	24.439634697739027
30-31	24.845778201123764	24.27011438879017	26.493402429252505	24.390704980833565
32-33	24.488422703489135	24.406866681666877	26.402839795923967	24.70187081892002
34-35	24.666837210564495	24.46175766525201	26.52764594842887	24.343759175754634
36-37	24.90413559222816	24.29314005060531	26.324578271303363	24.478146085863166
38-39	24.892886283224154	24.49053142795116	26.21523606484999	24.40134622397469
40-41	24.86366777955994	24.355807964478473	26.341455086357207	24.439069169604384
42-43	24.809455634564564	24.45284804376484	26.13323593721609	24.60446038445451
44-45	24.75593113263809	24.502410504016694	26.015606992108086	24.72605137123713
46-47	24.87619056911025	24.56178489249015	25.801540868317012	24.760483670082586
48-49	25.009124324060622	24.798098603681133	25.32445852434449	24.868318547913752
50-51	24.958176759785054	25.06848584168604	25.200320134151454	24.773017264377454
52-53	25.112525741730785	24.927203574479815	25.268271979032193	24.69199870475721
54-55	24.87770001326282	24.86425425591758	25.41443269422793	24.84361303659167
56-57	24.862495171056647	24.921890793160234	25.231004148851284	24.984609886931835
58-59	24.907586617347008	24.729406542050373	25.439291328691628	24.92371551191099
60-61	25.003201465478448	24.886058319114763	25.492446370879712	24.618293844527077
62-63	24.83000612560322	24.99072317182454	25.434821782754824	24.74444891981741
64-65	24.865937274377035	24.619459431771613	25.581741370226553	24.932861923624795
66-67	24.800518292682927	24.923719512195124	25.46359756097561	24.81216463414634
68-69	24.935419111064494	24.83421799190635	25.30454859239275	24.925814304636408
70-71	24.746533297478777	24.660647003979978	25.54518131245964	25.047638386081605
72-73	25.102589815443523	24.96834419321369	25.14506180085487	24.78400419048792
74-75	24.871850066712362	25.092689935092654	25.113087818835083	24.922372179359904
76-77	24.87583531051691	24.745710106167287	25.271180560001806	25.107274023313998
78-79	24.844858757510067	24.86416088644646	25.26005273475795	25.03092762128552
80-81	24.774567108476717	25.05358738173425	25.065051879903283	25.106793629885754
82-83	24.855870923761437	24.97099126048842	25.138610860945242	25.034526954804903
84-85	24.716764147768018	25.041037185910035	25.03228705785938	25.209911608462566
86-87	24.59200821471429	25.132745197514122	25.126037815003073	25.149208772768517
88-89	24.69863317766983	24.871879463027987	25.121961482124718	25.307525877177472
90-91	24.68940188941213	24.815897257346275	25.208645709919807	25.286055143321782
92-93	24.697751274359163	25.05304748664667	25.117070315358163	25.13213092363601
94-95	24.65499007201864	24.798338354994616	25.220974945146875	25.32569662783987
96-97	24.639335761387716	24.85858620654965	25.20523481777551	25.29684321428713
98-99	24.543019890040043	24.801137706022494	25.13278571831411	25.523056685623352
100-101	24.528298435329166	24.910373120381273	25.15775214962742	25.403576294662145
102-103	24.587531186666375	24.68667003226585	25.308147432755085	25.41765134831269
104-105	24.542788184971755	24.888736055615052	25.11633975690364	25.452136002509558
106-107	24.48017393382613	24.842485263668753	25.14858270270534	25.528758099799774
108-109	24.545137168512603	24.803238076363108	25.101342338153355	25.550282416970937
110-111	24.565026396863228	24.89621420388414	25.10241397622301	25.43634542302963
112-113	24.46489464353494	24.830589730964878	25.179150959358914	25.52536466614127
114-115	24.292700236597316	25.001912959868694	25.176838278220277	25.528548525313717
116-117	24.27125208607844	24.973308561564693	25.143456954377303	25.61198239797956
118-119	24.280142023437648	24.71388911415838	25.22047108220616	25.785497780197815
120-121	24.069430645416485	24.805203792547033	25.35406891543654	25.77129664659994
122-123	24.114552308648125	24.83850651064927	25.221091369800124	25.82584981090248
124-125	24.12837127044869	24.84975072799622	25.196810538031134	25.82506746352396
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	26.0
1	26.5
2	39.5
3	68.5
4	63.0
5	51.5
6	96.0
7	211.5
8	373.5
9	441.5
10	368.0
11	333.0
12	384.5
13	454.5
14	559.0
15	668.5
16	809.0
17	941.5
18	1074.0
19	1234.0
20	1412.0
21	1618.5
22	1823.5
23	2118.0
24	2414.5
25	2854.0
26	3659.0
27	4586.0
28	5376.5
29	6427.5
30	8065.0
31	9702.5
32	11626.0
33	13976.0
34	16382.0
35	18716.0
36	21388.5
37	24409.0
38	26799.5
39	29599.0
40	32535.0
41	35032.0
42	37569.0
43	41861.0
44	46978.5
45	50762.0
46	54648.0
47	58618.0
48	63711.0
49	68588.0
50	69670.5
51	69740.0
52	70162.5
53	70926.5
54	72085.5
55	71590.0
56	68367.5
57	63203.0
58	59222.5
59	55226.0
60	49237.5
61	43071.0
62	36754.0
63	28989.0
64	21989.5
65	17125.5
66	13104.0
67	9866.0
68	7842.0
69	6240.5
70	5257.0
71	4465.5
72	3560.5
73	3244.0
74	2659.0
75	1978.0
76	1367.5
77	750.5
78	438.5
79	286.5
80	153.0
81	65.0
82	34.0
83	10.0
84	5.0
85	4.5
86	3.5
87	2.0
88	2.0
89	1.0
90	0.0
91	0.0
92	1.0
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12303610176756176
2	0.0015242331735327273
3	0.01883952202486451
4	0.0582866765558915
5	0.024631608084288877
6	0.017254319524390476
7	0.013108405292381456
8	0.004999484809187346
9	0.0039020369242437824
10-11	0.006889533944367928
12-13	0.004267852885891637
14-15	0.0027436197123589094
16-17	0.009419761012432255
18-19	0.005029969472658
20-21	0.011675626109260692
22-23	0.007926012502370182
24-25	0.012285319378673783
26-27	0.008627159762195238
28-29	0.012925497311557529
30-31	0.019693092602042838
32-33	0.011706110772731346
34-35	0.01935776130386564
36-37	0.013565675244441274
38-39	0.019693092602042838
40-41	0.00911491437772571
42-43	0.007986981829311492
44-45	0.01603493298556429
46-47	0.01521184707185662
48-49	0.019601638611630876
50-51	0.014114399186913058
52-53	0.01963212327510153
54-55	0.015028939091032693
56-57	0.01935776130386564
58-59	0.01554717837003382
60-61	0.017985951447686183
62-63	0.020150362554102658
64-65	0.016156871639446912
66-67	0.010303816253081237
68-69	0.02225380433357782
70-71	0.012803558657674909
72-73	0.016126386975976258
74-75	0.017864012793803567
76-77	0.012803558657674909
78-79	0.027649589767883672
80-81	0.019754061928984146
82-83	0.008901521733431129
84-85	0.011767080099672655
86-87	0.011279325484142183
88-89	0.018717583370981894
90-91	0.011462233464966111
92-93	0.007865043175428873
94-95	0.006554202646190728
96-97	0.002164411106416473
98-99	0.0016156871639446911
100-101	0.010700116878199746
102-103	0.0027436197123589094
104-105	0.002286349760299091
106-107	3.048466347065455E-4
108-109	0.018687098707511236
110-111	7.316319232957091E-4
112-113	0.008627159762195238
114-115	0.0024692577411230183
116-117	0.007926012502370182
118-119	0.009633153656726836
120-121	0.003505736299125273
122-123	6.09693269413091E-4
124-125	0.024021914814875783
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1640169.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.2325147239081
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.95819555671494	44.40017894216189
2	10.833438486276393	12.1838297838018
3	3.65721325729556	6.169628950180335
4	1.829708632708101	4.115564705168802
5	1.123933260160637	3.1600796800336535
6	0.7225636433736937	2.4378942428983152
7	0.4869789054523761	1.916883392975837
8	0.37400012263321175	1.6824773922172414
9	0.2899953639653724	1.4676451716643102
>10	1.6125212259548143	16.242037601124153
>50	0.08379607636602429	3.190376605750301
>100	0.027002265084254396	2.5577943593692614
>500	2.177346714966801E-4	0.06581025364952767
>1k	4.354693429933602E-4	0.4097989190045937
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2308	0.1407172065805414	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2200	0.13413251927088002	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	3.0484663470654547E-5	0.0
14-15	0.0	0.0	0.0	6.0969326941309094E-5	3.0484663470654547E-5
16-17	0.0	0.0	0.0	6.0969326941309094E-5	1.2193865388261819E-4
18-19	0.0	0.0	0.0	6.0969326941309094E-5	3.0484663470654546E-4
20-21	0.0	0.0	0.0	6.0969326941309094E-5	3.0484663470654546E-4
22-23	0.0	0.0	0.0	9.145399041196364E-5	3.658159616478546E-4
24-25	0.0	0.0	0.0	2.4387730776523637E-4	4.8775461553047275E-4
26-27	0.0	0.0	0.0	3.353312981772E-4	4.8775461553047275E-4
28-29	0.0	0.0	0.0	0.0010364785580022546	4.8775461553047275E-4
30-31	0.0	0.0	0.0	0.003597190289537237	5.182392790011273E-4
32-33	0.0	0.0	0.0	0.00951121500284422	6.096932694130909E-4
34-35	0.0	0.0	0.0	0.017467712168685055	6.096932694130909E-4
36-37	0.0	0.0	0.0	0.03170405000948073	6.096932694130909E-4
38-39	0.0	0.0	0.0	0.05240313650605517	7.316319232957091E-4
40-41	0.0	0.0	0.0	0.07429112487798514	7.926012502370183E-4
42-43	0.0	0.0	0.0	0.0941671254608519	7.926012502370183E-4
44-45	0.0	0.0	0.0	0.11754886234284394	7.926012502370183E-4
46-47	0.0	0.0	0.0	0.14495457480296237	7.926012502370183E-4
48-49	0.0	0.0	0.0	0.1758660235622061	8.535705771783274E-4
50-51	0.0	0.0	0.0	0.2096125460242207	8.535705771783274E-4
52-53	0.0	0.0	0.0	0.2437248844478831	9.45024567590291E-4
54-55	0.0	0.0	0.0	0.2784774008044293	0.0010364785580022546
56-57	0.0	0.0	0.0	0.31935733451857706	0.0010669632214729092
58-59	0.0	0.0	0.0	0.36450512111861644	0.0011584172118848728
60-61	0.0	0.0	0.0	0.41514014714337366	0.0012193865388261818
62-63	0.0	0.0	0.0	0.4671469830243103	0.0012193865388261818
64-65	0.0	0.0	0.0	0.5204036901075437	0.0012193865388261818
66-67	0.0	0.0	0.0	0.5800316918561441	0.0012193865388261818
68-69	0.0	0.0	0.0	0.6537131234647161	0.001280355865767491
70-71	0.0	0.0	0.0	0.732790340507594	0.0013413251927088
72-73	0.0	0.0	0.0	0.8174767356290724	0.0017376258178273094
74-75	0.0	0.0	0.0	0.8993890263747212	0.0017681104812979638
76-77	0.0	0.0	0.0	0.9907210781328022	0.0017985951447686184
78-79	0.0	0.0	0.0	1.0976307929243876	0.001829079808239273
80-81	0.0	0.0	0.0	1.2162161338252337	0.001829079808239273
82-83	0.0	0.0	0.0	1.3426970025649796	0.001890049135180582
84-85	0.0	0.0	0.0	1.4702448345261983	0.001890049135180582
86-87	0.0	0.0	0.0	1.6077916361057916	0.0019205337986512366
88-89	0.0	0.0	0.0	1.7641779597102494	0.0020119877890632
90-91	0.0	0.0	0.0	1.9366906702906834	0.0020119877890632
92-93	0.0	0.0	0.0	2.1266406083763316	0.0021339264429458184
94-95	0.0	0.0	0.0	2.317444117039159	0.0021339264429458184
96-97	0.0	0.0	0.0	2.5192221045514214	0.002164411106416473
98-99	6.0969326941309094E-5	0.0	0.0	2.7406017306753148	0.0021948957698871277
100-101	6.0969326941309094E-5	0.0	0.0	2.9923745662794508	0.0021948957698871277
102-103	6.0969326941309094E-5	0.0	0.0	3.2736260714597094	0.0022558650968284365
104-105	6.0969326941309094E-5	0.0	0.0	3.562498742507632	0.002286349760299091
106-107	6.0969326941309094E-5	0.0	0.0	3.85954130336569	0.0023168344237697457
108-109	6.0969326941309094E-5	0.0	0.0	4.169143545573657	0.0024082884141817095
110-111	6.0969326941309094E-5	0.0	0.0	4.513041034186111	0.0026216810584762914
112-113	6.0969326941309094E-5	0.0	0.0	4.878674087853142	0.0027436197123589094
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2150	0.0	78.19677	1
GTATCAA	4150	0.0	59.761757	1
TCAACGC	5885	0.0	41.6906	4
CAACGCA	6085	0.0	40.681984	5
ATCAACG	6200	0.0	39.66971	3
AACGCAG	6350	0.0	38.975914	6
TATCAAC	6380	0.0	38.645023	2
GTACATG	8005	0.0	33.66319	1
ACGCAGA	7425	0.0	33.25078	7
TACATGG	8280	0.0	32.078823	2
CGCAGAG	7845	0.0	31.467745	8
ACATGGG	8520	0.0	30.824757	3
GTATACG	100	4.388192E-4	29.80905	1
GCGCGAC	250	2.1827873E-11	28.552998	9
GCAGAGT	8510	0.0	28.379644	9
AGAGTAC	7655	0.0	27.665283	10-11
CATGGGG	7130	0.0	27.478546	4
GTATAGG	450	0.0	26.496933	1
CCTATAC	340	0.0	26.273203	3
CAGAGTA	8245	0.0	24.783716	10-11
>>END_MODULE