FastQCFastQC Report
Mon 15 Aug 2016
SRR2921504_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921504_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1739448
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18400.059.513881
GTATCAA33250.054.0547141
TCAACGC41600.041.6143464
CAACGCA42800.040.5865825
AACGCAG44950.038.7776346
GTACATG85950.038.5680431
ATCAACG45400.038.261143
TATCAAC47550.036.6562462
TACATGG90300.036.2331282
ACATGGG91200.035.288513
CATGGGG74800.032.8467064
ACGCAGA54550.031.7343147
GTATAGG4350.030.0989741
CGCAGAG59100.029.3901168
ATGGGGG38400.028.1957445
GCAGAGT61950.026.5016969
ATAGGAC4300.024.902043
AGAGTAC56750.024.89451410-11
ATGGGGA32300.023.943315
CAGAGTA60150.022.79508610-11
Produced by FastQC (version 0.11.5)