##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921504_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1739448
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.179388518656495	31.0	30.0	34.0	23.0	34.0
2	29.182361875721494	31.0	30.0	34.0	22.0	34.0
3	29.32337155235454	31.0	30.0	34.0	23.0	34.0
4	32.71040525500044	37.0	33.0	37.0	24.0	37.0
5	32.681615661980125	37.0	33.0	37.0	24.0	37.0
6	32.67938506928635	37.0	33.0	37.0	23.0	37.0
7	32.46972315355216	37.0	33.0	37.0	22.0	37.0
8	32.39430152554144	37.0	33.0	37.0	19.0	37.0
9	33.802796059439544	39.0	34.0	39.0	19.0	39.0
10-11	33.69673252664064	38.5	34.0	39.0	18.5	39.0
12-13	33.56621612143623	39.0	34.0	39.0	17.0	39.0
14-15	34.639103037285395	39.5	34.0	41.0	17.0	41.0
16-17	34.54442357575506	39.0	34.0	41.0	17.0	41.0
18-19	34.549493000078186	39.5	34.0	41.0	17.5	41.0
20-21	34.572673342347684	40.0	34.0	41.0	18.0	41.0
22-23	34.5280876461958	39.0	34.0	41.0	18.0	41.0
24-25	34.4688771955241	39.0	34.0	41.0	18.0	41.0
26-27	34.37492296406676	39.0	34.0	41.0	17.5	41.0
28-29	34.32128525831183	39.0	34.0	41.0	17.0	41.0
30-31	34.22055071493945	39.0	34.0	41.0	16.0	41.0
32-33	34.115990820076256	39.0	33.0	41.0	16.0	41.0
34-35	33.94261282889744	39.0	33.0	41.0	15.0	41.0
36-37	33.79351265458927	39.0	33.0	41.0	13.0	41.0
38-39	33.66268293159669	39.0	33.0	41.0	9.0	41.0
40-41	33.51100435310512	39.0	33.0	41.0	9.0	41.0
42-43	33.30731990838473	38.0	33.0	41.0	8.0	41.0
44-45	33.10761344978407	38.0	32.0	40.5	6.5	41.0
46-47	32.85573929200528	38.0	32.0	40.0	2.0	41.0
48-49	32.71802462620326	38.0	32.0	40.0	2.0	41.0
50-51	32.52847598778463	38.0	32.0	40.0	2.0	41.0
52-53	32.324601252811235	37.5	31.0	40.0	2.0	41.0
54-55	32.121104798763746	37.0	31.0	40.0	2.0	41.0
56-57	31.877540748559312	37.0	31.0	40.0	2.0	41.0
58-59	31.62427706950711	36.0	31.0	40.0	2.0	41.0
60-61	31.320742269961507	36.0	30.5	39.5	2.0	41.0
62-63	31.008228759928436	35.0	30.0	39.0	2.0	41.0
64-65	30.705647998675442	35.0	30.0	39.0	2.0	40.5
66-67	30.67665460536906	35.0	30.0	39.0	2.0	40.0
68-69	30.436900959384815	35.0	30.0	38.0	2.0	40.0
70-71	30.145818673510217	35.0	30.0	37.0	2.0	40.0
72-73	29.82944589317991	35.0	30.0	37.0	2.0	39.0
74-75	29.536605290873887	35.0	29.0	36.0	2.0	39.0
76-77	29.271491300688496	35.0	29.0	36.0	2.0	38.5
78-79	28.99946448528499	34.5	29.0	35.0	2.0	37.0
80-81	28.765405749410156	34.0	29.0	35.0	2.0	37.0
82-83	28.56950912013466	34.0	29.0	35.0	2.0	36.0
84-85	28.37079636758328	34.0	29.0	35.0	2.0	36.0
86-87	28.224476385611986	34.0	29.0	35.0	2.0	36.0
88-89	28.09766000478313	34.0	29.0	35.0	2.0	35.0
90-91	27.987460389732835	34.0	28.5	35.0	2.0	35.0
92-93	27.885784168310867	34.0	28.0	35.0	2.0	35.0
94-95	27.81870886626102	34.0	28.0	35.0	2.0	35.0
96-97	27.716468672820344	34.0	27.0	35.0	2.0	35.0
98-99	27.658673613698138	34.0	27.0	35.0	2.0	35.0
100-101	27.593064868854945	34.0	27.0	35.0	2.0	35.0
102-103	26.624107188027466	32.5	25.0	34.5	2.0	35.0
104-105	27.437780548771794	34.0	27.0	35.0	2.0	35.0
106-107	27.766792683655964	34.0	28.5	35.0	2.0	35.0
108-109	27.80913255239593	34.0	29.0	35.0	2.0	35.0
110-111	27.75111845826952	34.0	29.0	35.0	2.0	35.0
112-113	27.675175400471872	34.0	28.5	35.0	2.0	35.0
114-115	27.547630052752368	34.0	27.5	35.0	2.0	35.0
116-117	27.48137541334952	34.0	27.0	35.0	2.0	35.0
118-119	27.319159296512456	34.0	27.0	35.0	2.0	35.0
120-121	27.142545508690112	34.0	27.0	35.0	2.0	35.0
122-123	27.01278279086239	34.0	27.0	35.0	2.0	35.0
124-125	26.164120744052134	33.0	23.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	92343.0
3	15250.0
4	9865.0
5	8430.0
6	8748.0
7	9881.0
8	10759.0
9	10969.0
10	11332.0
11	11715.0
12	11404.0
13	11683.0
14	12571.0
15	10727.0
16	8433.0
17	7252.0
18	6846.0
19	6294.0
20	6137.0
21	6489.0
22	7737.0
23	10479.0
24	14699.0
25	20054.0
26	23554.0
27	25573.0
28	29717.0
29	34786.0
30	40027.0
31	47882.0
32	59280.0
33	75895.0
34	104261.0
35	161253.0
36	297945.0
37	405191.0
38	103882.0
39	105.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.094345319396716	16.96858510775955	11.9386942331747	24.99837533966903
2	16.93575980066579	19.311795531771622	37.814097731498016	25.938346936064576
3	19.142781893231295	24.103638122249098	28.81106905566156	27.94251092885805
4	13.355128658552365	15.025317656801263	35.49440596237287	36.125147722273496
5	14.390923635560595	37.267130039117895	33.373800785118405	14.968145540203102
6	33.88483655634685	34.51486850081846	17.146888203551967	14.453406739282729
7	30.086187957151022	30.928628365764528	20.64496611949449	18.34021755758996
8	28.09162781362271	33.39632635291696	19.330268797694064	19.18177703576627
9	27.904418339984048	14.37376610993892	18.58433237813781	39.137483171939216
10-11	26.482530086530044	24.722972732051314	26.91535284313827	21.87914433828037
12-13	26.632685806827837	22.44933638980873	27.027982162636544	23.889995640726884
14-15	24.126582839015885	23.632145581651734	24.184897393126647	28.056374186205733
16-17	24.42467167947762	26.587355788709704	24.668717684464358	24.31925484734832
18-19	24.495416326826422	25.812251734479414	26.123342164386433	23.568989774307735
20-21	25.636142634931154	24.77010046204256	25.68872068514837	23.905036217877917
22-23	25.38237117873286	24.437156527431213	25.578575479696795	24.601896814139135
24-25	24.773162584575946	24.936338829014133	25.653560696644345	24.636937889765573
26-27	24.535090083081716	24.946112926599405	25.78305574720197	24.73574124311691
28-29	24.5680150997355	24.968285781417926	25.827524691938535	24.636174426908035
30-31	24.891463272943124	24.92666437361022	25.606269937224702	24.575602416221955
32-33	24.466742807574708	24.998051417834404	25.660137933732102	24.87506784085879
34-35	24.77638241748256	25.08407676024888	25.70291622345741	24.43662459881115
36-37	24.922052975504172	24.822773156830767	25.566365193853045	24.688808673812016
38-39	24.908837171309035	25.059955487113527	25.46145159870753	24.569755742869916
40-41	24.886172389873657	24.959334956867448	25.666803175439462	24.487689477819433
42-43	24.77233624344879	24.95356412924617	25.54795477960147	24.726144847703573
44-45	24.70014855592994	24.985065311399403	25.54816850527999	24.766617627390662
46-47	24.85290327628182	25.08174596965332	25.41963122845611	24.64571952560875
48-49	24.84027102733856	25.211581932277678	25.212646025181613	24.73550101520215
50-51	24.90704801522204	25.36746519477352	25.089385757639203	24.636101032365236
52-53	24.945810869580225	25.234560699879655	25.19673760725651	24.62289082328361
54-55	24.776400809385933	25.145289146107046	25.33831779786288	24.739992246644142
56-57	24.745176441086038	25.27161815979488	25.203490746289948	24.779714652829142
58-59	24.832734730007324	25.079135561006204	25.211366072319564	24.876763636666904
60-61	24.83258896478905	25.209364436168148	25.334822070421904	24.623224528620906
62-63	24.66305130523405	25.26813259622751	25.3732691011404	24.695546997398043
64-65	24.753048112410486	25.019626743904492	25.388293200835797	24.83903194284922
66-67	24.639674443922697	25.28356326555612	25.292678697695177	24.78408359282601
68-69	24.792483038069022	25.185779006655956	25.25409829466316	24.76763966061186
70-71	24.63882110518513	25.033597869522637	25.456874945177884	24.870706080114346
72-73	24.834152876384767	25.22434757405001	25.23906973213436	24.70242981743086
74-75	24.626128085979975	25.35674735101271	25.264294840402236	24.752829722605085
76-77	24.680450825140205	25.09250334702816	25.31141164075691	24.91563418707472
78-79	24.677222107363455	25.171514169849562	25.308644992769647	24.84261873001733
80-81	24.674484520224425	25.33554991889068	25.121232700655717	24.868732860229176
82-83	24.7902026227655	25.200985441762597	25.21211176811701	24.7967001673549
84-85	24.643490775941057	25.24689922291181	25.14334113133621	24.966268869810918
86-87	24.483358896697656	25.369858996603213	25.292522033128396	24.85426007357074
88-89	24.6430052138231	25.192075356908532	25.256507790832938	24.908411638435428
90-91	24.52062989744632	25.175314096337154	25.307958582952107	24.99609742326442
92-93	24.577735265477145	25.42107159173011	25.187273105210185	24.81392003758256
94-95	24.55550748115875	25.09403016612499	25.364240219295915	24.98622213342035
96-97	24.56612926270507	25.177769926402462	25.31288513223898	24.943215678653495
98-99	24.475527088951115	25.192149579996205	25.22270837765269	25.10961495339999
100-101	24.463520917265246	25.204095771930195	25.33432379458585	24.998059516218706
102-103	24.473873256988217	24.998296766270624	25.5181208258197	25.009709150921456
104-105	24.382738230415647	25.26068871149031	25.35469015457057	25.001882903523477
106-107	24.30983649725349	25.213246953656014	25.37049430287392	25.106422246216574
108-109	24.2842456419969	25.23254757608897	25.350186923518326	25.133019858395805
110-111	24.348225291606873	25.294941494646206	25.351975015638224	25.004858198108693
112-113	24.298549853893	25.270862110351388	25.406145836776993	25.02444219897862
114-115	24.163096532051295	25.457170314609755	25.433684036834077	24.946049116504874
116-117	24.085497756163974	25.404691084823067	25.407738251805807	25.102072907207152
118-119	24.253765964178463	25.135991950267776	25.438577281290538	25.171664804263223
120-121	23.974104815435794	25.263555998149105	25.610886801314088	25.15145238510101
122-123	24.01162188894512	25.315907208906474	25.465939412062383	25.206531490086025
124-125	23.986644781662587	25.342892212522173	25.516630782542205	25.15383222327304
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	68.0
1	89.5
2	116.5
3	130.0
4	131.0
5	113.5
6	111.0
7	152.5
8	211.0
9	255.0
10	270.0
11	301.0
12	356.5
13	402.5
14	474.5
15	566.0
16	646.5
17	696.0
18	763.0
19	860.0
20	955.0
21	1111.5
22	1345.0
23	1676.0
24	2067.5
25	2635.5
26	3646.5
27	4856.0
28	5738.0
29	7018.5
30	9153.0
31	11132.0
32	13411.0
33	16063.5
34	19034.5
35	22138.0
36	25603.0
37	29080.5
38	31602.5
39	34554.5
40	37182.0
41	39239.0
42	41921.0
43	46375.0
44	51481.0
45	55638.5
46	59292.0
47	62799.0
48	67374.0
49	71296.0
50	71737.5
51	71848.0
52	71745.5
53	71915.5
54	73646.5
55	73304.0
56	69978.0
57	64833.5
58	60729.5
59	56485.0
60	50347.0
61	43971.5
62	37918.5
63	30362.0
64	23048.0
65	18175.0
66	14103.5
67	11119.0
68	8997.5
69	7117.0
70	5853.0
71	4694.0
72	3679.5
73	3195.0
74	2603.5
75	2046.0
76	1497.5
77	906.0
78	554.0
79	339.0
80	190.5
81	103.0
82	67.0
83	40.0
84	24.0
85	18.5
86	21.5
87	18.5
88	13.0
89	8.5
90	9.5
91	9.5
92	8.0
93	6.5
94	5.0
95	8.0
96	6.5
97	3.0
98	3.5
99	4.5
100	16.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03581596000570296
2	0.02638768160933814
3	0.027250024145591013
4	0.031216799812354267
5	0.03460868045494893
6	0.0463365389479881
7	0.03966775666763249
8	0.03627587602503783
9	0.030871862797853113
10-11	0.03621838652262097
12-13	0.03598842851295354
14-15	0.034637425206157355
16-17	0.03659206828833055
18-19	0.05093569914133679
20-21	0.06208866261020738
22-23	0.05484498530568318
24-25	0.06573924601367791
26-27	0.04935473782487318
28-29	0.05027456986354292
30-31	0.04978590909299962
32-33	0.057748205177734546
34-35	0.040788801964761236
36-37	0.05332151349163643
38-39	0.06180121509812308
40-41	0.04909603506399732
42-43	0.05915669798694758
44-45	0.06010527477682575
46-47	0.06530807474555146
48-49	0.050475783122001926
50-51	0.05266038421384256
52-53	0.06277853663920968
54-55	0.048636119044662446
56-57	0.045646664918985794
58-59	0.046997668225781976
60-61	0.03529855448395123
62-63	0.04346206382714516
64-65	0.043059637310227154
66-67	0.03670704729316427
68-69	0.03184918433893971
70-71	0.05004461185387549
72-73	0.0329127401336516
74-75	0.0412487179840961
76-77	0.03403378543078034
78-79	0.03434997769407307
80-81	0.025122912556167245
82-83	0.018942791046354934
84-85	0.018827812041521216
86-87	0.017563042988350326
88-89	0.02397312250783007
90-91	0.01221651926358247
92-93	0.019747644080190956
94-95	0.014113672843338806
96-97	0.011009239712828437
98-99	0.010233131430200847
100-101	0.010980494961620008
102-103	0.006295100514646026
104-105	0.0064963137731050315
106-107	0.008623425362528803
108-109	0.013452543565544933
110-111	0.006898740290023042
112-113	0.008077275089568644
114-115	0.007732338075067493
116-117	0.007329911558149482
118-119	0.2434680427353965
120-121	0.35815960005702957
122-123	0.028054877179427035
124-125	0.0030469436280935102
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1739448.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.066414585715336
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.27131883588251	48.004054122880916
2	9.584569074635072	11.322522611756423
3	3.278752380028935	5.809924422080699
4	1.6187154505589954	3.8244687159608244
5	0.9657447613685094	2.852154022948755
6	0.642017171211955	2.2752991443572115
7	0.4575189099851354	1.8916801132590608
8	0.34426050108747835	1.6267386786175282
9	0.2564402121245499	1.3632303497217706
>10	1.4800328066970978	15.659458999555673
>50	0.07922443162938939	3.1552165281637374
>100	0.021012770181701217	2.017456394711837
>500	2.945209564939477E-4	0.13580709129233598
>1k	9.817365216464923E-5	0.06198880469321399
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.7489502416858684E-5
2	0.0	0.0	0.0	0.0	5.7489502416858684E-5
3	0.0	0.0	0.0	0.0	5.7489502416858684E-5
4	0.0	0.0	0.0	0.0	5.7489502416858684E-5
5	0.0	0.0	0.0	0.0	5.7489502416858684E-5
6	0.0	0.0	0.0	0.0	1.1497900483371737E-4
7	0.0	0.0	0.0	0.0	1.1497900483371737E-4
8	0.0	0.0	0.0	0.0	1.7246850725057605E-4
9	0.0	0.0	0.0	0.0	1.7246850725057605E-4
10-11	0.0	0.0	0.0	0.0	1.7246850725057605E-4
12-13	0.0	0.0	0.0	0.0	2.012132584590054E-4
14-15	0.0	0.0	0.0	5.7489502416858684E-5	3.1619226329272277E-4
16-17	0.0	0.0	0.0	5.7489502416858684E-5	4.3117126812644015E-4
18-19	0.0	0.0	0.0	5.7489502416858684E-5	5.174055217517282E-4
20-21	0.0	0.0	0.0	5.7489502416858684E-5	5.174055217517282E-4
22-23	0.0	0.0	0.0	8.623425362528802E-5	7.473635314191628E-4
24-25	0.0	0.0	0.0	2.012132584590054E-4	8.048530338360216E-4
26-27	0.0	0.0	0.0	2.874475120842934E-4	8.048530338360216E-4
28-29	0.0	0.0	0.0	0.0010348110435034561	9.198320386697389E-4
30-31	0.0	0.0	0.0	0.003765562408304244	0.001006066292295027
32-33	0.0	0.0	0.0	0.009054596630655241	0.0010348110435034563
34-35	0.0	0.0	0.0	0.016614466198472157	0.0010348110435034563
36-37	0.0	0.0	0.0	0.028543537949970336	0.0010348110435034563
38-39	0.0	0.0	0.0	0.0473138604890747	0.0010635557947118856
40-41	0.0	2.8744751208429342E-5	0.0	0.06706150456926566	0.0010923005459203149
42-43	0.0	5.7489502416858684E-5	0.0	0.08735529892241678	0.0011210452971287441
44-45	0.0	5.7489502416858684E-5	0.0	0.11037984464036868	0.0012360243019624616
46-47	0.0	5.7489502416858684E-5	0.0	0.13912459584879802	0.0014084928092130376
48-49	0.0	5.7489502416858684E-5	0.0	0.170255161407527	0.0016384508188804726
50-51	0.0	5.7489502416858684E-5	0.0	0.20236304850734255	0.0016671955700889019
52-53	0.0	5.7489502416858684E-5	0.0	0.23332114555882094	0.0018109193261310486
54-55	0.0	5.7489502416858684E-5	0.0	0.26657882270697375	0.0019833878333816246
56-57	0.0	5.7489502416858684E-5	0.0	0.3057579186040629	0.0020121325845900537
58-59	0.0	5.7489502416858684E-5	0.0	0.35011106971866934	0.0020121325845900537
60-61	0.0	5.7489502416858684E-5	0.0	0.39958078654837625	0.0020121325845900537
62-63	0.0	5.7489502416858684E-5	0.0	0.45051648568971303	0.0020696220870069127
64-65	0.0	5.7489502416858684E-5	0.0	0.5053614709953962	0.0020983668382153417
66-67	0.0	5.7489502416858684E-5	0.0	0.568542434151524	0.002127111589423771
68-69	0.0	5.7489502416858684E-5	0.0	0.6391682878706348	0.002127111589423771
70-71	0.0	5.7489502416858684E-5	0.0	0.714766983548804	0.0022420905942574887
72-73	0.0	5.7489502416858684E-5	0.0	0.798586678072584	0.0023858143502996356
74-75	0.0	5.7489502416858684E-5	0.0	0.8818314775721954	0.0024145591015080647
76-77	0.0	5.7489502416858684E-5	0.0	0.9717450593521623	0.0024145591015080647
78-79	0.0	5.7489502416858684E-5	0.0	1.075571100717009	0.0024145591015080647
80-81	0.0	5.7489502416858684E-5	0.0	1.191383703335771	0.0024145591015080647
82-83	0.0	5.7489502416858684E-5	0.0	1.3144687280102652	0.0024145591015080647
84-85	0.0	5.7489502416858684E-5	0.0	1.4398820775326424	0.0024145591015080647
86-87	0.0	5.7489502416858684E-5	0.0	1.574206299929633	0.0024720486039249232
88-89	0.0	5.7489502416858684E-5	0.0	1.7279619741435215	0.0025582828575502117
90-91	0.0	5.7489502416858684E-5	0.0	1.904282278056027	0.0025870276087586407
92-93	0.0	5.7489502416858684E-5	0.0	2.0892547520822697	0.0026445171111754992
94-95	0.0	5.7489502416858684E-5	0.0	2.2847190602995893	0.0027594961160092167
96-97	0.0	5.7489502416858684E-5	0.0	2.4839201861740046	0.002960709374468222
98-99	0.0	5.7489502416858684E-5	0.0	2.7034438511527794	0.0031044331305103688
100-101	0.0	5.7489502416858684E-5	0.0	2.96001950043922	0.0031044331305103688
102-103	0.0	5.7489502416858684E-5	0.0	3.2357966435328906	0.003133177881718798
104-105	0.0	5.7489502416858684E-5	0.0	3.521375746788636	0.0031619226329272273
106-107	0.0	5.7489502416858684E-5	0.0	3.8096281119067656	0.0031906673841356568
108-109	0.0	5.7489502416858684E-5	0.0	4.114121261457658	0.0032481568865525157
110-111	0.0	1.1497900483371737E-4	0.0	4.448307739006857	0.0034206253938030918
112-113	0.0	1.1497900483371737E-4	0.0	4.8256400881199095	0.003449370145011521
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1840	0.0	59.51388	1
GTATCAA	3325	0.0	54.054714	1
TCAACGC	4160	0.0	41.614346	4
CAACGCA	4280	0.0	40.586582	5
AACGCAG	4495	0.0	38.777634	6
GTACATG	8595	0.0	38.568043	1
ATCAACG	4540	0.0	38.26114	3
TATCAAC	4755	0.0	36.656246	2
TACATGG	9030	0.0	36.233128	2
ACATGGG	9120	0.0	35.28851	3
CATGGGG	7480	0.0	32.846706	4
ACGCAGA	5455	0.0	31.734314	7
GTATAGG	435	0.0	30.098974	1
CGCAGAG	5910	0.0	29.390116	8
ATGGGGG	3840	0.0	28.195744	5
GCAGAGT	6195	0.0	26.501696	9
ATAGGAC	430	0.0	24.90204	3
AGAGTAC	5675	0.0	24.894514	10-11
ATGGGGA	3230	0.0	23.94331	5
CAGAGTA	6015	0.0	22.795086	10-11
>>END_MODULE