FastQCFastQC Report
Mon 15 Aug 2016
SRR2921504_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921504_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1739448
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22690.13044368098385234No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21080.12118787109473811No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22850.076.423471
GTATCAA47550.062.2947121
TCAACGC62850.046.5073364
CAACGCA63900.045.6302765
ATCAACG66700.043.9121323
AACGCAG67150.043.5915226
TATCAAC70100.041.7009772
ACGCAGA80550.035.89567
GTACATG89000.035.291161
CGCAGAG84750.034.255138
TACATGG92300.033.606152
ACATGGG91650.033.4518433
GCAGAGT89850.031.648669
CATGGGG76650.030.988894
AGAGTAC84700.027.989610-11
ATGGGGG38550.027.630365
TACCGTA1807.3006777E-726.4416147
CAGAGTA87300.026.06567410-11
ACCGTAT2451.6249032E-824.2817178
GAGTACT47350.023.37092612-13
Produced by FastQC (version 0.11.5)