##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921504_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1739448
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25241225952141	31.0	31.0	34.0	28.0	34.0
2	31.302772488743557	33.0	31.0	34.0	28.0	34.0
3	31.38949195376924	33.0	31.0	34.0	28.0	34.0
4	34.9314891850748	37.0	35.0	37.0	32.0	37.0
5	34.59500312742893	37.0	35.0	37.0	31.0	37.0
6	34.57453341519839	37.0	35.0	37.0	31.0	37.0
7	34.41184789657409	37.0	35.0	37.0	30.0	37.0
8	34.37290163316178	37.0	35.0	37.0	30.0	37.0
9	35.953672084477375	39.0	37.0	39.0	30.0	39.0
10-11	35.824112304593186	39.0	36.0	39.0	30.0	39.0
12-13	35.7412345755665	39.0	35.0	39.0	30.0	39.0
14-15	36.91257197685702	40.0	37.0	41.0	30.0	41.0
16-17	36.890205111046726	40.0	37.0	41.0	30.0	41.0
18-19	36.89731857462827	40.0	37.0	41.0	30.0	41.0
20-21	36.87230316744163	40.0	37.0	41.0	30.0	41.0
22-23	36.8133229622271	40.0	37.0	41.0	30.0	41.0
24-25	36.74821063923728	40.0	36.5	41.0	30.0	41.0
26-27	36.62184066439468	40.0	36.0	41.0	30.0	41.0
28-29	36.43155213607995	40.0	36.0	41.0	29.0	41.0
30-31	36.3895718641776	40.0	36.0	41.0	29.0	41.0
32-33	36.27404038522566	40.0	35.5	41.0	29.0	41.0
34-35	36.15447774236424	40.0	35.0	41.0	29.0	41.0
36-37	36.032840015913095	40.0	35.0	41.0	28.0	41.0
38-39	35.85797304662169	40.0	35.0	41.0	27.5	41.0
40-41	35.650798126762055	39.5	35.0	41.0	27.0	41.0
42-43	35.49056022370315	39.0	35.0	41.0	26.0	41.0
44-45	35.28678494556894	39.0	35.0	41.0	25.5	41.0
46-47	35.02458883507872	39.0	35.0	41.0	24.5	41.0
48-49	34.77969361544582	39.0	34.0	41.0	24.0	41.0
50-51	34.5667412880408	39.0	34.0	41.0	23.0	41.0
52-53	34.3669741780151	38.0	34.0	41.0	23.0	41.0
54-55	34.14927408005298	38.0	34.0	40.0	22.5	41.0
56-57	33.88292377811811	38.0	33.0	40.0	22.0	41.0
58-59	33.73636607705433	37.0	33.0	40.0	21.5	41.0
60-61	33.61533227782607	37.0	33.0	40.0	22.0	41.0
62-63	33.34186535038702	36.5	33.0	40.0	21.0	41.0
64-65	33.04785598649686	36.0	33.0	39.0	20.0	41.0
66-67	32.74313920278158	35.5	33.0	39.0	20.0	41.0
68-69	32.42397818158405	35.0	32.5	39.0	20.0	41.0
70-71	32.122829196388736	35.0	32.0	37.5	18.5	40.0
72-73	31.80791607452479	35.0	32.0	37.0	18.0	39.5
74-75	31.525757884110362	35.0	32.0	36.5	18.0	39.0
76-77	31.233159312609516	35.0	32.0	36.0	16.0	39.0
78-79	30.956150456926565	35.0	31.5	36.0	14.0	37.0
80-81	30.73115292897517	35.0	31.0	35.0	12.5	37.0
82-83	30.5458516149951	35.0	31.0	35.0	11.0	36.5
84-85	30.354409272366865	35.0	31.0	35.0	10.0	36.0
86-87	30.214126837939393	35.0	31.0	35.0	8.0	36.0
88-89	30.115005737452343	35.0	31.0	35.0	7.0	36.0
90-91	30.013453118460568	35.0	31.0	35.0	7.0	35.0
92-93	30.072750378280926	35.0	31.0	35.0	7.0	35.0
94-95	30.044234435292115	35.0	31.0	35.0	7.0	35.0
96-97	30.021715797195434	35.0	31.0	35.0	5.0	35.0
98-99	29.982324565034425	35.0	31.0	35.0	2.0	35.0
100-101	29.93539703400159	35.0	31.0	35.0	2.0	35.0
102-103	29.04195152715114	33.5	29.5	35.0	2.0	35.0
104-105	29.752461413045978	34.5	31.0	35.0	2.0	35.0
106-107	29.862092456917367	35.0	31.0	35.0	2.0	35.0
108-109	29.86917200169249	35.0	31.0	35.0	2.0	35.0
110-111	29.837716332997594	35.0	31.0	35.0	2.0	35.0
112-113	29.791273151022622	35.0	31.0	35.0	2.0	35.0
114-115	29.728268680638916	35.0	31.0	35.0	2.0	35.0
116-117	29.65046123827789	35.0	31.0	35.0	2.0	35.0
118-119	29.555598385234855	35.0	31.0	35.0	2.0	35.0
120-121	29.42456112513855	35.0	31.0	35.0	2.0	35.0
122-123	29.30750588692505	35.0	31.0	35.0	2.0	35.0
124-125	28.3835205191532	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	26683.0
3	10104.0
4	5969.0
5	4882.0
6	5353.0
7	6326.0
8	7157.0
9	8129.0
10	9124.0
11	9734.0
12	10362.0
13	11689.0
14	14351.0
15	12106.0
16	7160.0
17	6052.0
18	5403.0
19	5034.0
20	4960.0
21	5340.0
22	6186.0
23	8301.0
24	11727.0
25	15927.0
26	20074.0
27	23230.0
28	26772.0
29	31904.0
30	37281.0
31	45300.0
32	55861.0
33	71741.0
34	98649.0
35	155142.0
36	309219.0
37	490494.0
38	155418.0
39	304.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.03812004432229	17.56296498827186	12.245556978601545	25.153357988804302
2	17.24668625935942	19.73486625457623	37.054852640874216	25.96359484519013
3	19.1258096883724	24.31195535609038	29.03383656442	27.52839839111722
4	13.474791784227543	15.496637543206319	35.29282509069804	35.7357455818681
5	14.33511120056355	37.128538363116206	33.48282752771029	15.053522908609956
6	33.879295028039834	34.93246347461638	16.604883007797564	14.583358489546228
7	29.674599012884023	31.355479045634567	20.554125785989946	18.415796155491464
8	28.22880626070079	33.21461136810248	19.0965862116942	19.45999615950253
9	27.891649246746557	14.004063498195919	18.62223954632319	39.482047708734335
10-11	26.50947298703158	24.69013399044063	26.828501952320433	21.97189107020736
12-13	26.858923629686203	22.359544974095556	27.157134450996296	23.624396945221942
14-15	24.290633866178375	23.367070923677925	24.568602481883172	27.773692728260524
16-17	24.292501578958415	26.339958586299673	25.067218925997803	24.30032090874411
18-19	24.277646072662705	25.25308797949597	27.024394815235787	23.444871132605538
20-21	25.39341715500032	24.220093971647515	26.802905623579846	23.583583249772317
22-23	25.064558925201336	23.960553152772448	26.662354593292427	24.31253332873379
24-25	24.52761572541499	24.496452608056813	26.725449198800277	24.25048246772792
26-27	24.247809539853165	24.548418375516405	26.798112002879314	24.405660081751115
28-29	24.37577638948023	24.52682106725398	26.85349557459793	24.24390696866786
30-31	24.643475080775517	24.50296967532815	26.629325062288718	24.22423018160762
32-33	24.291506239379313	24.547394530527924	26.65383727505562	24.50726195503714
34-35	24.48375825542592	24.581740899732534	26.703346906574644	24.231153938266907
36-37	24.699551141494357	24.4635216576457	26.468996046721767	24.367931154138176
38-39	24.687415634781516	24.693625777381914	26.33468471019766	24.284273877638913
40-41	24.652772387607033	24.580644716166546	26.449467221149938	24.317115675076483
42-43	24.609821816401585	24.606688374614752	26.31711638263981	24.46637342634385
44-45	24.636640756551966	24.643339417710973	26.16675296343882	24.55326686229824
46-47	24.742282267795296	24.831118232043565	25.88435627419091	24.542243225970232
48-49	24.877212623775684	24.97082515014374	25.44446543251086	24.707496793569717
50-51	24.75109188383598	25.193508954712406	25.384804242880044	24.67059491857157
52-53	24.96505322088262	25.104064061715636	25.42782850403635	24.503054213365395
54-55	24.758625943883747	25.058886271082407	25.53707757923017	24.64541020580367
56-57	24.77131665714059	25.134467185636595	25.407196653874507	24.68701950334831
58-59	24.706540882455933	24.9822760180293	25.530813854202783	24.780369245311984
60-61	24.78993601910859	25.134421972580274	25.602275412811014	24.47336659550012
62-63	24.655065985189164	25.14248321439487	25.653045071071258	24.549405729344713
64-65	24.709064469554047	24.831999429600184	25.741879133889285	24.717056966956484
66-67	24.66469453524107	25.08910526754579	25.554194804037405	24.692005393175737
68-69	24.823514156388608	25.035242540269074	25.456399162632064	24.684844140710243
70-71	24.621110084133875	24.86883999327274	25.759971941001353	24.75007798159203
72-73	24.888573024595154	25.101407675674835	25.38485179630088	24.62516750342913
74-75	24.695262721800265	25.256695767574538	25.33627619929838	24.711765311326815
76-77	24.688106236495337	25.00779087564842	25.390377492361495	24.913725395494744
78-79	24.66718115811181	25.055062469305906	25.340380946027853	24.93737542655443
80-81	24.613797570729147	25.225614785736035	25.207013012328634	24.953574631206184
82-83	24.757034968508766	25.139345372129547	25.218084239376502	24.88553541998518
84-85	24.62668717388492	25.201885897454325	25.131021000733085	25.040405927927665
86-87	24.479513442093605	25.20276120921921	25.325084893499337	24.992640455187846
88-89	24.583202038765055	25.055632479477797	25.252516830621957	25.10864865113519
90-91	24.461850669651906	25.039435679522683	25.31665693531801	25.1820567155074
92-93	24.47673648113135	25.283583831546075	25.246011255674333	24.99366843164824
94-95	24.4830808830695	24.995148964868157	25.417929253366044	25.1038408986963
96-97	24.472162064427707	25.00927040077452	25.37977023203885	25.13879730275893
98-99	24.516362331855067	25.00304698917227	25.248185891635266	25.232404787337405
100-101	24.395794385672186	25.033800556735347	25.391886658964097	25.17851839862837
102-103	24.410331561925354	24.839102937612843	25.514541460978958	25.236024039482842
104-105	24.336980071098882	25.060041962540712	25.343155446233588	25.25982252012681
106-107	24.28773217188941	24.998763972855695	25.380092719282715	25.333411135972185
108-109	24.36241149575516	24.936670247997323	25.310223065053624	25.390695191193895
110-111	24.351356214683257	25.010822467269584	25.29904791032961	25.338773407717554
112-113	24.274000987757667	25.00990344927508	25.374448123461658	25.341647439505593
114-115	24.087771266940035	25.130885085869757	25.381803861372337	25.399539785817872
116-117	24.04432016700986	25.084682129030757	25.347632968119044	25.523364735840342
118-119	24.07695416867661	24.861249179971587	25.424503052719277	25.637293598632528
120-121	23.890727981860245	24.944463097280416	25.4946291330724	25.67017978778694
122-123	23.89435985911505	24.99221729373385	25.4048516370416	25.708571210109497
124-125	23.869178268487538	25.031037542344176	25.380141608247847	25.71964258092044
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	21.5
2	48.5
3	75.5
4	57.0
5	50.0
6	94.0
7	191.0
8	327.0
9	392.0
10	353.5
11	332.5
12	380.0
13	449.5
14	533.5
15	682.0
16	791.0
17	911.0
18	1095.0
19	1312.0
20	1507.5
21	1697.5
22	1942.0
23	2293.0
24	2695.5
25	3246.5
26	4271.5
27	5437.5
28	6285.0
29	7584.0
30	9594.5
31	11477.5
32	13758.0
33	16169.5
34	18882.5
35	21913.5
36	25426.0
37	28796.5
38	31148.5
39	34062.0
40	36650.5
41	38584.0
42	40973.5
43	45645.5
44	51268.0
45	55082.5
46	58554.5
47	62548.0
48	67378.5
49	71309.0
50	71949.0
51	72032.0
52	71964.5
53	72575.5
54	74463.5
55	74032.5
56	70281.0
57	64938.5
58	61142.5
59	57074.0
60	50607.5
61	44149.0
62	37509.0
63	29557.5
64	22449.5
65	17459.5
66	13499.0
67	10427.5
68	8382.5
69	6653.5
70	5449.5
71	4422.0
72	3487.0
73	3108.0
74	2544.0
75	1882.0
76	1293.5
77	788.0
78	490.5
79	278.5
80	136.0
81	54.0
82	31.5
83	16.0
84	11.5
85	8.5
86	4.0
87	2.5
88	1.5
89	1.0
90	0.5
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1249246887518339
2	0.0013797480580046084
3	0.020293794353151116
4	0.056742138885439525
5	0.027192534643174154
6	0.018109193261310486
7	0.013797480580046083
8	0.005174055217517281
9	0.003449370145011521
10-11	0.006180121509812308
12-13	0.004282967930055972
14-15	0.0024720486039249232
16-17	0.009686981157240688
18-19	0.004742883949390841
20-21	0.011497900483371737
22-23	0.008364722601652937
24-25	0.01221651926358247
26-27	0.008307233099236078
28-29	0.013078861799835351
30-31	0.021213626391820853
32-33	0.01221651926358247
34-35	0.020954923630944988
36-37	0.014861036374757969
38-39	0.020552497114026978
40-41	0.009830704913282834
42-43	0.008422212104069796
44-45	0.01693065846176488
46-47	0.016528231944846872
48-49	0.020954923630944988
50-51	0.014487354609048387
52-53	0.021558563406322007
54-55	0.016700700452097445
56-57	0.020811199874902844
58-59	0.01603957117430357
60-61	0.01925898330964766
62-63	0.022047224176865304
64-65	0.018224172266144204
66-67	0.0122452640147909
68-69	0.024576762283207085
70-71	0.014688567867507394
72-73	0.017505553485933467
74-75	0.018799067290312787
76-77	0.013452543565544933
78-79	0.02954960424226536
80-81	0.021069902635778705
82-83	0.008882128123404666
84-85	0.012820159038959486
86-87	0.012072795507540323
88-89	0.020466262860401693
90-91	0.012848903790167916
92-93	0.008479701606486655
94-95	0.00738740106056634
96-97	0.0023570695990912057
98-99	0.0014947270628383256
100-101	0.011584134736997025
102-103	0.0034206253938030913
104-105	0.002270835345465918
106-107	2.2995800966743474E-4
108-109	0.019345217563272947
110-111	6.323845265854455E-4
112-113	0.008910872874613096
114-115	0.0023283248478827767
116-117	0.008594680611320373
118-119	0.010290620932617704
120-121	0.0036793281546789558
122-123	8.335977850444509E-4
124-125	0.02443303852716494
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1739448.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.093400669274715
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.56115293222003	44.62855629136459
2	10.363376577580095	11.626340693055544
3	3.591793751480251	6.0442777806953725
4	1.8016317427274042	4.042386048131679
5	1.0377727302891195	2.910610078187737
6	0.6877339176770515	2.314640051886531
7	0.5112496395179666	2.00744116100525
8	0.3702589838085656	1.6615268424137897
9	0.2987759679301057	1.5083424071508411
>10	1.6552106342157695	16.78791672758602
>50	0.09212315817260397	3.481946763576957
>100	0.028508360446911865	2.617641896519678
>500	1.0290098353711756E-4	0.05373793537992089
>1k	3.087029506113527E-4	0.31463532304613184
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2269	0.13044368098385234	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2108	0.12118787109473811	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	5.7489502416858684E-5
3	0.0	0.0	0.0	0.0	5.7489502416858684E-5
4	0.0	0.0	0.0	0.0	5.7489502416858684E-5
5	0.0	0.0	0.0	0.0	5.7489502416858684E-5
6	0.0	0.0	0.0	0.0	1.1497900483371737E-4
7	0.0	0.0	0.0	0.0	1.1497900483371737E-4
8	0.0	0.0	0.0	0.0	1.1497900483371737E-4
9	0.0	0.0	0.0	0.0	1.1497900483371737E-4
10-11	0.0	0.0	0.0	0.0	1.1497900483371737E-4
12-13	0.0	0.0	0.0	0.0	3.449370145011521E-4
14-15	0.0	0.0	0.0	0.0	4.024265169180108E-4
16-17	0.0	0.0	0.0	0.0	4.5991601933486947E-4
18-19	0.0	0.0	0.0	0.0	4.5991601933486947E-4
20-21	0.0	0.0	0.0	0.0	5.461502729601575E-4
22-23	0.0	0.0	0.0	8.623425362528802E-5	6.611292777938748E-4
24-25	0.0	0.0	0.0	2.012132584590054E-4	7.186187802107336E-4
26-27	0.0	0.0	0.0	2.874475120842934E-4	7.473635314191628E-4
28-29	0.0	0.0	0.0	0.001092300545920315	7.473635314191628E-4
30-31	0.0	0.0	0.0	0.004110499422805396	7.473635314191628E-4
32-33	0.0	0.0	0.0	0.009859449664491263	8.335977850444508E-4
34-35	0.0	0.0	0.0	0.017649277241975615	9.773215410865976E-4
36-37	0.0	0.0	0.0	0.02975081750072437	9.773215410865976E-4
38-39	0.0	0.0	0.0	0.04941222732729004	9.773215410865976E-4
40-41	0.0	0.0	0.0	0.07030966145581817	9.773215410865976E-4
42-43	0.0	0.0	0.0	0.09103462707709573	0.0010348110435034561
44-45	0.0	0.0	0.0	0.11546766560426067	0.0011210452971287441
46-47	0.0	0.0	0.0	0.1453622068610272	0.001178534799545603
48-49	0.0	2.8744751208429342E-5	0.0	0.17787252047776075	0.001264769053170891
50-51	0.0	5.7489502416858684E-5	0.0	0.21136015563558094	0.0013222585555877496
52-53	0.0	5.7489502416858684E-5	0.0	0.24366925599385553	0.0014947270628383256
54-55	0.0	5.7489502416858684E-5	0.0	0.2778755099318864	0.0015522165652551844
56-57	0.0	5.7489502416858684E-5	0.0	0.31806067212127065	0.0015809613164636136
58-59	0.0	5.7489502416858684E-5	0.0	0.36330491052333846	0.0016384508188804726
60-61	0.0	5.7489502416858684E-5	0.0	0.4148155046888438	0.0016671955700889019
62-63	0.0	5.7489502416858684E-5	0.0	0.46782082591718754	0.0017246850725057604
64-65	0.0	5.7489502416858684E-5	0.0	0.5239305802760416	0.0017246850725057604
66-67	0.0	5.7489502416858684E-5	0.0	0.5888937180070919	0.0017246850725057604
68-69	0.0	5.7489502416858684E-5	0.0	0.6619916203301277	0.0017246850725057604
70-71	0.0	5.7489502416858684E-5	0.0	0.7401485988658472	0.0017246850725057604
72-73	0.0	5.7489502416858684E-5	0.0	0.8259804259742172	0.0018109193261310486
74-75	0.0	5.7489502416858684E-5	0.0	0.9110648895511679	0.0018396640773394779
76-77	0.0	5.7489502416858684E-5	0.0	1.002990603915725	0.0018684088285479072
78-79	0.0	5.7489502416858684E-5	0.0	1.1111858474642529	0.0018971535797563364
80-81	0.0	5.7489502416858684E-5	0.0	1.2303328412231926	0.0019833878333816246
82-83	0.0	5.7489502416858684E-5	0.0	1.3568672360426985	0.0021846010918406297
84-85	0.0	5.7489502416858684E-5	0.0	1.4854712529492113	0.0021846010918406297
86-87	0.0	5.7489502416858684E-5	0.0	1.6237047615105482	0.0022708353454659177
88-89	0.0	5.7489502416858684E-5	0.0	1.7808523163670316	0.002357069599091206
90-91	0.0	5.7489502416858684E-5	0.0	1.964272573828019	0.002357069599091206
92-93	0.0	5.7489502416858684E-5	0.0	2.155913830134617	0.0024145591015080647
94-95	0.0	5.7489502416858684E-5	0.0	2.3566384278230794	0.002529538106341782
96-97	0.0	5.7489502416858684E-5	0.0	2.5625945702314756	0.0025870276087586407
98-99	0.0	5.7489502416858684E-5	0.0	2.7898218285341096	0.0025870276087586407
100-101	0.0	5.7489502416858684E-5	0.0	3.0533249628617813	0.0025870276087586407
102-103	0.0	5.7489502416858684E-5	0.0	3.3357421434846	0.002702006613592358
104-105	0.0	5.7489502416858684E-5	0.0	3.6285074345424526	0.002702006613592358
106-107	0.0	5.7489502416858684E-5	0.0	3.9244059034820244	0.0027594961160092167
108-109	0.0	5.7489502416858684E-5	0.0	4.238787247448617	0.0028744751208429342
110-111	0.0	5.7489502416858684E-5	0.0	4.583436814437684	0.0031619226329272273
112-113	0.0	5.7489502416858684E-5	0.0	4.972612001048608	0.0031619226329272273
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2285	0.0	76.42347	1
GTATCAA	4755	0.0	62.294712	1
TCAACGC	6285	0.0	46.507336	4
CAACGCA	6390	0.0	45.630276	5
ATCAACG	6670	0.0	43.912132	3
AACGCAG	6715	0.0	43.591522	6
TATCAAC	7010	0.0	41.700977	2
ACGCAGA	8055	0.0	35.8956	7
GTACATG	8900	0.0	35.29116	1
CGCAGAG	8475	0.0	34.25513	8
TACATGG	9230	0.0	33.60615	2
ACATGGG	9165	0.0	33.451843	3
GCAGAGT	8985	0.0	31.64866	9
CATGGGG	7665	0.0	30.98889	4
AGAGTAC	8470	0.0	27.9896	10-11
ATGGGGG	3855	0.0	27.63036	5
TACCGTA	180	7.3006777E-7	26.441614	7
CAGAGTA	8730	0.0	26.065674	10-11
ACCGTAT	245	1.6249032E-8	24.281717	8
GAGTACT	4735	0.0	23.370926	12-13
>>END_MODULE