FastQCFastQC Report
Mon 15 Aug 2016
SRR2921503_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921503_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1576720
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15050.078.2961651
GTATCAA30000.061.4967651
TCAACGC40450.045.5949974
CAACGCA41500.044.4413875
AACGCAG43650.041.9771466
TATCAAC43900.041.8762662
ATCAACG44100.041.821263
GTACATG78350.037.4472351
TACATGG82400.035.3787842
ACGCAGA53450.034.390897
ACATGGG85550.033.6588553
GTATACG1102.0372023E-532.4616361
CGCAGAG56400.032.2746248
CATGGGG74550.031.6025094
GCAGAGT61750.028.8040189
GTATATA5100.026.8391951
ATGGGGG40200.026.3431645
CAGAGTA59200.025.0715710-11
AGAGTAC55500.024.86724510-11
ATGGGGA29100.024.3292085
Produced by FastQC (version 0.11.5)