##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921503_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1576720
Sequences flagged as poor quality	0
Sequence length	125
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.080618626008423	31.0	30.0	34.0	26.0	34.0
2	30.094958521487644	31.0	30.0	34.0	26.0	34.0
3	30.25223311685017	31.0	31.0	34.0	26.0	34.0
4	33.67551499315034	37.0	35.0	37.0	28.0	37.0
5	33.65943921558679	37.0	35.0	37.0	28.0	37.0
6	33.67447041960526	37.0	35.0	37.0	28.0	37.0
7	33.49570120249632	37.0	35.0	37.0	27.0	37.0
8	33.43086280379522	37.0	35.0	37.0	27.0	37.0
9	34.907586001319196	39.0	35.0	39.0	27.0	39.0
10-11	34.825280328783805	39.0	35.0	39.0	27.0	39.0
12-13	34.701944860216145	39.0	35.0	39.0	26.5	39.0
14-15	35.8786208077528	40.0	36.0	41.0	27.0	41.0
16-17	35.81126008422548	40.0	36.0	41.0	26.5	41.0
18-19	35.82479958394642	40.0	36.0	41.0	26.0	41.0
20-21	35.857076716221016	40.0	36.0	41.0	26.5	41.0
22-23	35.81633073722665	40.0	36.0	41.0	26.0	41.0
24-25	35.778412463849	40.0	36.0	41.0	26.0	41.0
26-27	35.714628786341265	40.0	36.0	41.0	26.0	41.0
28-29	35.68142092445076	40.0	36.0	41.0	26.0	41.0
30-31	35.585960094373135	40.0	35.0	41.0	26.0	41.0
32-33	35.48732273326907	40.0	35.0	41.0	25.0	41.0
34-35	35.33347582322797	40.0	35.0	41.0	25.0	41.0
36-37	35.19997304530925	40.0	35.0	41.0	25.0	41.0
38-39	35.07124822416155	39.5	35.0	41.0	24.0	41.0
40-41	34.93340574103202	39.0	35.0	41.0	24.0	41.0
42-43	34.74102884469025	39.0	34.0	41.0	23.0	41.0
44-45	34.53766267948653	39.0	34.0	41.0	23.0	41.0
46-47	34.27937046526967	39.0	33.5	41.0	21.0	41.0
48-49	34.12982520675833	38.0	33.0	40.5	20.5	41.0
50-51	33.94138115835405	38.0	33.0	40.0	19.0	41.0
52-53	33.734831802729715	38.0	33.0	40.0	18.5	41.0
54-55	33.52104939367801	38.0	33.0	40.0	18.0	41.0
56-57	33.27820443705921	37.0	33.0	40.0	17.0	41.0
58-59	33.019363298493076	37.0	32.5	40.0	15.0	41.0
60-61	32.70457468669136	36.0	32.0	40.0	13.0	41.0
62-63	32.38799374651175	36.0	32.0	39.0	11.5	41.0
64-65	32.070727205845046	35.0	32.0	39.0	8.0	41.0
66-67	32.02684401796134	35.0	32.0	39.0	8.0	41.0
68-69	31.776441600284134	35.0	32.0	38.0	7.5	40.0
70-71	31.481469760008117	35.0	32.0	37.0	7.0	40.0
72-73	31.15175459181085	35.0	31.0	37.0	3.5	39.0
74-75	30.85456612461312	35.0	31.0	36.0	2.0	39.0
76-77	30.58539689989345	35.0	31.0	36.0	2.0	38.5
78-79	30.32567133035669	35.0	31.0	35.0	2.0	37.0
80-81	30.0937284362474	35.0	31.0	35.0	2.0	37.0
82-83	29.904410738748794	35.0	31.0	35.0	2.0	36.0
84-85	29.71142530062408	35.0	30.0	35.0	2.0	36.0
86-87	29.564593903800294	35.0	30.0	35.0	2.0	36.0
88-89	29.447863602922524	34.0	30.0	35.0	2.0	35.0
90-91	29.346961730681414	34.0	30.0	35.0	2.0	35.0
92-93	29.249169478410877	34.0	30.0	35.0	2.0	35.0
94-95	29.18628894160028	34.0	30.0	35.0	2.0	35.0
96-97	29.08421913846466	34.0	30.0	35.0	2.0	35.0
98-99	29.034058995890202	34.0	30.0	35.0	2.0	35.0
100-101	28.964192120351107	34.0	30.0	35.0	2.0	35.0
102-103	27.97639688720889	33.0	28.0	34.5	2.0	35.0
104-105	28.819341100512457	34.0	29.5	35.0	2.0	35.0
106-107	29.15466347861383	34.5	30.0	35.0	2.0	35.0
108-109	29.19485958191689	35.0	31.0	35.0	2.0	35.0
110-111	29.138902595261047	35.0	30.5	35.0	2.0	35.0
112-113	29.068179511898116	35.0	30.0	35.0	2.0	35.0
114-115	28.95316701760617	35.0	30.0	35.0	2.0	35.0
116-117	28.893275597442795	35.0	30.0	35.0	2.0	35.0
118-119	28.739189583439035	35.0	30.0	35.0	2.0	35.0
120-121	28.564670011162413	35.0	30.0	35.0	2.0	35.0
122-123	28.441482634836877	34.5	30.0	35.0	2.0	35.0
124-125	27.5761603201583	34.0	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	54041.0
3	9801.0
4	6488.0
5	5708.0
6	5972.0
7	6869.0
8	7613.0
9	8036.0
10	8324.0
11	8729.0
12	8693.0
13	9251.0
14	9932.0
15	8561.0
16	6577.0
17	5772.0
18	5401.0
19	4919.0
20	4857.0
21	5049.0
22	5911.0
23	7895.0
24	11406.0
25	16115.0
26	19524.0
27	20612.0
28	24325.0
29	28917.0
30	32634.0
31	40424.0
32	51146.0
33	67519.0
34	96120.0
35	152605.0
36	297084.0
37	411667.0
38	102133.0
39	90.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.967363305290135	16.735632621467637	12.206656769068736	25.090347304173488
2	16.848276006442653	18.963072732336148	38.280694431316526	25.90795682990467
3	19.574310976793402	23.38885357551121	28.459262529799627	28.57757291789576
4	13.734299556749344	14.791769887756779	34.75454368401119	36.71938687148268
5	14.816316250589207	36.77398971875993	32.951038179397045	15.458655851253816
6	34.52039236586975	33.564603319628446	16.945269275671286	14.969735038830517
7	30.46107111225031	30.75831326340794	20.00011420189372	18.780501422448033
8	28.098080341678628	33.35287377761198	19.066398175886835	19.482647704822558
9	27.841112388376715	14.757683260123175	18.441727505957047	38.959476845543065
10-11	26.803537350400454	24.60293945976673	26.358418432085106	22.23510475774771
12-13	26.982124486861338	22.10557436478025	26.61113883238015	24.301162315978267
14-15	24.414995057815315	23.246254027024044	23.926993971676648	28.41175694348399
16-17	24.698211953008173	26.330344901531475	24.3402599561347	24.631183189325647
18-19	24.83969757719754	25.487696584815218	25.756382195366704	23.916223642620537
20-21	25.87785678250854	24.44793461327343	25.531060916137587	24.14314768808044
22-23	25.784978441369727	24.08700231925809	25.35440865264113	24.773610586731053
24-25	25.13329652686555	24.490579448029905	25.429810700528616	24.946313324575932
26-27	24.795363789423856	24.673189602852993	25.534024289535374	24.997422318187773
28-29	24.85119113714019	24.748590590825597	25.523202507590348	24.877015764443865
30-31	25.094842557230717	24.59891385523019	25.354037637536774	24.952205950002316
32-33	24.766746102176423	24.72959300062218	25.349202667789406	25.154458229411997
34-35	25.048798090268136	24.779817750672134	25.39128090030216	24.780103258757563
36-37	25.1545954122814	24.56111908328117	25.361642697807728	24.922642806629703
38-39	25.124790112739444	24.76948889675768	25.287908843884495	24.817812146618387
40-41	25.159080172853848	24.680086813977166	25.31964351828205	24.841189494886944
42-43	25.044054946052125	24.715443439921874	25.221226583614285	25.019275030411713
44-45	24.962758702089605	24.711976833136458	25.289879282669936	25.035385182103997
46-47	25.051609197955305	24.728849430601255	25.174722439926768	25.044818931516673
48-49	25.164879023149687	24.880266801353297	24.93045702929688	25.02439714620014
50-51	25.070401265001912	25.094164468015173	24.91916069255301	24.916273574429905
52-53	25.24109532189089	24.910634339131974	24.972696910641954	24.875573428335183
54-55	25.025895804483106	24.87221828299316	25.104416007969405	24.997469904554332
56-57	24.994836800053296	24.93652674474123	24.984748335641306	25.08388811956417
58-59	25.09633242029297	24.799673615037058	24.963341968829727	25.14065199584024
60-61	25.08366593516469	24.949197726951187	25.130804353991127	24.836331983892997
62-63	24.9285821013184	25.036474282735888	25.04881478804944	24.986128827896266
64-65	24.952470020344386	24.839311710695412	25.10896285507901	25.099255413881195
66-67	24.97968208248345	24.873223173528654	25.132391613981774	25.014703130006122
68-69	24.90781977592529	24.971419689645106	25.090879677210616	25.029880857218988
70-71	24.97495245884684	24.714324782376686	25.193353058338086	25.117369700438385
72-73	25.04489284242668	24.989793837095366	24.994710558929768	24.970602761548193
74-75	24.83292992034312	25.182519263895543	25.034403778864124	24.95014703689722
76-77	25.026185849427087	24.821584118591606	25.011847867669314	25.140382164312
78-79	24.88217175738389	24.937112803611136	25.02155452708511	25.15916091191986
80-81	24.92409699851683	25.182033984752156	24.827798831988247	25.066070184742767
82-83	25.00953876162611	25.041984823616165	24.92434802603931	25.024128388718413
84-85	24.95254370498788	25.116519675054818	24.821140911179896	25.109795708777412
86-87	24.75427493423544	25.186763736657582	24.97147083618519	25.087490492921788
88-89	24.770803993960847	25.049258427536508	24.932757330085387	25.247180248417262
90-91	24.76178968226784	24.994164465655345	24.98398399541276	25.260061856664052
92-93	24.84194614386017	25.072243449721064	24.981691348325548	25.104119058093215
94-95	24.86413832058948	24.92588858374963	25.005621589185996	25.204351506474897
96-97	24.76257560395153	24.93675290007418	25.079500898954016	25.221170597020276
98-99	24.78102682861389	24.970521317575002	24.91467197948956	25.333779874321543
100-101	24.706678950815306	25.065562150444876	25.041268713637148	25.186490185102667
102-103	24.66283424418643	24.86329275219288	25.179060093471193	25.294812910149496
104-105	24.612213314390097	25.047506088897908	25.02908083011975	25.311199766592246
106-107	24.646691128666525	24.993419362613732	25.028368097069954	25.33152141164979
108-109	24.62660011531677	24.999318121414984	25.01168708179435	25.362394681473898
110-111	24.61574517180074	25.098200394133535	25.110473461315735	25.175580972749984
112-113	24.628105096879622	25.13025478848284	25.07878396939278	25.162856145244756
114-115	24.393522791060537	25.32926763520349	25.033505538800142	25.243704034935828
116-117	24.37738524955649	25.313662100560887	25.0675655533335	25.24138709654913
118-119	24.582869112876693	25.09727570801235	25.01437560295681	25.305479576154138
120-121	24.413757198356407	25.122550267567554	25.191256065458585	25.27243646861746
122-123	24.40301130221629	25.249218436772043	25.037143284900097	25.31062697611157
124-125	24.35852550929802	25.29175228962123	25.019724991754828	25.329997209325928
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	145.0
1	164.5
2	197.0
3	202.5
4	185.5
5	163.5
6	160.5
7	203.5
8	256.0
9	280.5
10	284.5
11	308.5
12	363.5
13	439.0
14	483.0
15	519.5
16	598.0
17	676.0
18	742.0
19	799.0
20	899.5
21	1013.0
22	1194.5
23	1479.5
24	1784.0
25	2254.0
26	2942.5
27	3692.5
28	4567.0
29	5793.5
30	7268.0
31	8758.0
32	10582.5
33	12191.5
34	13738.0
35	15844.5
36	18469.0
37	21211.0
38	23931.0
39	27048.0
40	29849.5
41	32793.0
42	36365.5
43	40676.5
44	45094.5
45	48770.5
46	53142.5
47	57659.5
48	61857.5
49	66060.0
50	67672.0
51	68631.0
52	68813.0
53	69299.0
54	71163.0
55	70674.5
56	66845.0
57	61532.5
58	57757.5
59	53405.0
60	47876.5
61	42638.5
62	36706.5
63	29227.5
64	22218.0
65	17293.5
66	13419.0
67	10426.0
68	8283.5
69	6515.0
70	5321.5
71	4249.5
72	3291.5
73	2800.5
74	2234.5
75	1604.0
76	1064.0
77	635.0
78	379.0
79	230.5
80	127.0
81	67.5
82	54.5
83	36.5
84	26.0
85	17.5
86	15.0
87	14.0
88	8.5
89	8.0
90	7.0
91	7.0
92	10.0
93	10.0
94	6.0
95	5.5
96	8.5
97	7.5
98	6.0
99	7.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.036785225024100664
2	0.02226140341975747
3	0.022705363032117305
4	0.026066771525698917
5	0.02860368359632655
6	0.04059059313004211
7	0.03577046019584961
8	0.03183824648637679
9	0.02765234156984119
10-11	0.03247247450403369
12-13	0.03256760870668223
14-15	0.03133086407225126
16-17	0.03367750773758182
18-19	0.04531559186158608
20-21	0.05486072352732254
22-23	0.04978689938606727
24-25	0.05771474960677863
26-27	0.04385686742097519
28-29	0.044713075244812016
30-31	0.043571464813029585
32-33	0.05127733522756102
34-35	0.03694378202851489
36-37	0.04766223552691663
38-39	0.05543152874321376
40-41	0.044808209447460556
42-43	0.0537191130955401
44-45	0.05381424729818864
46-47	0.059300319650920905
48-49	0.04541072606423461
50-51	0.04782079253133086
52-53	0.056509716373230504
54-55	0.04392029022274088
56-57	0.042017606169770155
58-59	0.04230300877771576
60-61	0.032409051702268
62-63	0.038656197676188546
64-65	0.038656197676188546
66-67	0.03329697092698767
68-69	0.029174488812217767
70-71	0.045474148866000305
72-73	0.02958673702369476
74-75	0.037102339032929116
76-77	0.030886904459891423
78-79	0.030760058856360035
80-81	0.02321274544624283
82-83	0.01633137145466538
84-85	0.017092445075853672
86-87	0.016680196864376683
88-89	0.021563752600334874
90-91	0.010908721903698819
92-93	0.01772667309351058
94-95	0.013223654168146533
96-97	0.010496473692221828
98-99	0.009957379877213455
100-101	0.010306205286924755
102-103	0.005359226749200872
104-105	0.005073824141255264
106-107	0.00792785022071135
108-109	0.012240600740778325
110-111	0.006152011771272007
112-113	0.006247145973920544
114-115	0.007293622203054442
116-117	0.006532548581866152
118-119	0.2373598356080978
120-121	0.35060124816073873
122-123	0.024861738292150794
124-125	0.002536912070627632
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1576720.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.04840359272768
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.94288588104328	48.00442675752258
2	10.448673463064887	12.548523222374877
3	3.548193865430782	6.391901317698843
4	1.758778975584915	4.224474790253085
5	1.0363874720432815	3.111670659985087
6	0.6901285045245649	2.4864668982322
7	0.4834034100102646	2.0319322143678034
8	0.35438307717746154	1.7024110435827977
9	0.2746796942108069	1.4844669423027803
>10	1.3942411530959706	14.42666097467312
>50	0.05475628747500545	2.2012827773063486
>100	0.013169631852909874	1.2276370194585156
>500	2.123896572626143E-4	0.0899133020004973
>1k	1.0619482863130715E-4	0.06823208024151213
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.34228017656908E-5
2	0.0	0.0	0.0	0.0	6.34228017656908E-5
3	0.0	0.0	0.0	0.0	6.34228017656908E-5
4	0.0	0.0	0.0	0.0	6.34228017656908E-5
5	0.0	0.0	0.0	0.0	6.34228017656908E-5
6	0.0	0.0	0.0	0.0	6.34228017656908E-5
7	0.0	0.0	0.0	0.0	6.34228017656908E-5
8	0.0	0.0	0.0	0.0	6.34228017656908E-5
9	0.0	0.0	0.0	0.0	6.34228017656908E-5
10-11	0.0	0.0	0.0	0.0	6.34228017656908E-5
12-13	0.0	0.0	0.0	0.0	6.34228017656908E-5
14-15	0.0	0.0	0.0	0.0	9.51342026485362E-5
16-17	0.0	0.0	0.0	0.0	1.268456035313816E-4
18-19	0.0	0.0	0.0	0.0	1.268456035313816E-4
20-21	0.0	0.0	0.0	0.0	1.268456035313816E-4
22-23	0.0	0.0	0.0	0.0	2.536912070627632E-4
24-25	0.0	0.0	0.0	0.0	4.122482114769902E-4
26-27	0.0	0.0	0.0	1.268456035313816E-4	5.073824141255264E-4
28-29	0.0	0.0	0.0	7.610736211882896E-4	5.073824141255264E-4
30-31	0.0	0.0	0.0	0.0028857374803389316	5.073824141255264E-4
32-33	0.0	0.0	0.0	0.008466944035719721	5.073824141255264E-4
34-35	0.0	0.0	0.0	0.014872647014054493	5.708052158912172E-4
36-37	0.0	0.0	0.0	0.026193617129230302	6.025166167740626E-4
38-39	0.0	0.0	0.0	0.04182733776447309	7.293622203054442E-4
40-41	0.0	0.0	0.0	0.060885889695063165	7.610736211882896E-4
42-43	0.0	0.0	0.0	0.07969075041859049	7.610736211882896E-4
44-45	0.0	0.0	0.0	0.10157161702775382	7.610736211882896E-4
46-47	0.0	0.0	0.0	0.12925566999847785	7.92785022071135E-4
48-49	0.0	0.0	0.0	0.15732025977979602	8.244964229539804E-4
50-51	0.0	0.0	0.0	0.18681186260084226	8.244964229539804E-4
52-53	0.0	0.0	0.0	0.21516185499010604	8.244964229539804E-4
54-55	0.0	0.0	0.0	0.25105916078948703	8.562078238368258E-4
56-57	0.0	0.0	0.0	0.28641737277385965	8.879192247196712E-4
58-59	0.0	0.0	0.0	0.32881551575422396	8.879192247196712E-4
60-61	0.0	0.0	0.0	0.37910979755441676	8.879192247196712E-4
62-63	0.0	0.0	0.0	0.4280404891166472	9.513420264853621E-4
64-65	0.0	0.0	0.0	0.48052285757775637	9.830534273682075E-4
66-67	0.0	0.0	0.0	0.5428040489116648	0.0010147648282510528
68-69	0.0	0.0	0.0	0.6144718149068953	0.0011416104317824344
70-71	0.0	0.0	0.0	0.6910865594398499	0.0013318788370795067
72-73	0.0	0.0	0.0	0.7717286518849257	0.00158557004414227
74-75	0.0	6.34228017656908E-5	0.0	0.854717387995332	0.0016172814450251154
76-77	0.0	6.34228017656908E-5	0.0	0.9440801156831904	0.0016489928459079608
78-79	0.0	6.34228017656908E-5	0.0	1.0487911613983458	0.0016489928459079608
80-81	0.0	6.34228017656908E-5	0.0	1.1671698208940078	0.0016807042467908062
82-83	0.0	6.34228017656908E-5	0.0	1.2915736465574104	0.0018392612512050331
84-85	0.0	6.34228017656908E-5	0.0	1.4178167334720178	0.0018392612512050331
86-87	0.0	6.34228017656908E-5	0.0	1.5583616621847887	0.0018709726520878787
88-89	0.0	6.34228017656908E-5	0.0	1.71707722360343	0.0019026840529707241
90-91	6.34228017656908E-5	6.34228017656908E-5	0.0	1.8921558678776194	0.0019343954538535695
92-93	6.34228017656908E-5	6.34228017656908E-5	0.0	2.0818217565579173	0.001966106854736415
94-95	6.34228017656908E-5	6.34228017656908E-5	0.0	2.2786227104368564	0.0021563752600334872
96-97	6.34228017656908E-5	6.34228017656908E-5	0.0	2.4882667816733472	0.002219798061799178
98-99	6.34228017656908E-5	6.34228017656908E-5	0.0	2.7148764523821605	0.002283220863564869
100-101	6.34228017656908E-5	6.34228017656908E-5	0.0	2.97097772591202	0.002283220863564869
102-103	6.34228017656908E-5	6.34228017656908E-5	0.0	3.250101476482825	0.0024100664670962503
104-105	6.34228017656908E-5	6.34228017656908E-5	0.0	3.5452394844994672	0.002473489268861941
106-107	9.51342026485362E-5	6.34228017656908E-5	0.0	3.853188898472779	0.002536912070627632
108-109	1.268456035313816E-4	6.34228017656908E-5	0.0	4.176137805063677	0.0026003348723933227
110-111	1.268456035313816E-4	1.268456035313816E-4	0.0	4.528261200466792	0.002695469075041859
112-113	1.268456035313816E-4	1.268456035313816E-4	0.0	4.905182911360292	0.0027271804759247046
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1505	0.0	78.296165	1
GTATCAA	3000	0.0	61.496765	1
TCAACGC	4045	0.0	45.594997	4
CAACGCA	4150	0.0	44.441387	5
AACGCAG	4365	0.0	41.977146	6
TATCAAC	4390	0.0	41.876266	2
ATCAACG	4410	0.0	41.82126	3
GTACATG	7835	0.0	37.447235	1
TACATGG	8240	0.0	35.378784	2
ACGCAGA	5345	0.0	34.39089	7
ACATGGG	8555	0.0	33.658855	3
GTATACG	110	2.0372023E-5	32.461636	1
CGCAGAG	5640	0.0	32.274624	8
CATGGGG	7455	0.0	31.602509	4
GCAGAGT	6175	0.0	28.804018	9
GTATATA	510	0.0	26.839195	1
ATGGGGG	4020	0.0	26.343164	5
CAGAGTA	5920	0.0	25.07157	10-11
AGAGTAC	5550	0.0	24.867245	10-11
ATGGGGA	2910	0.0	24.329208	5
>>END_MODULE