FastQCFastQC Report
Mon 15 Aug 2016
SRR2921503_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921503_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1576720
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28710.1820868638692983No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24870.15773250799127303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22150.077.526411
GTATCAA45700.061.4519461
TCAACGC62850.045.0028274
CAACGCA64850.043.6762355
ATCAACG65700.042.960023
TATCAAC66250.042.6932452
AACGCAG68650.041.166716
TATACCG2001.8189894E-1235.7025685
ACGCAGA80350.035.094927
GTACATG80150.034.146021
CGCAGAG84650.033.3111348
TACATGG83500.032.4471362
ACATGGG85050.031.4357223
GCAGAGT90950.030.4804389
CATGGGG73650.029.5101594
GTGGTAT8700.029.471
AGAGTAC82650.027.4606210-11
CAGAGTA86450.025.73743610-11
ATGGGGG40000.025.5868425
GAGTACT48000.024.54084812-13
Produced by FastQC (version 0.11.5)