##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921503_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1576720
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.914003120401848	31.0	31.0	34.0	27.0	34.0
2	30.987503171140087	31.0	31.0	34.0	27.0	34.0
3	31.068226444771422	31.0	31.0	34.0	27.0	34.0
4	34.58883695266122	37.0	35.0	37.0	31.0	37.0
5	34.22153457811152	37.0	35.0	37.0	30.0	37.0
6	34.202378355066216	37.0	35.0	37.0	30.0	37.0
7	34.0332398904054	37.0	35.0	37.0	30.0	37.0
8	34.00048328174945	37.0	35.0	37.0	30.0	37.0
9	35.53205642092445	39.0	35.0	39.0	30.0	39.0
10-11	35.388157377340306	39.0	35.0	39.0	29.0	39.0
12-13	35.289153432442035	39.0	35.0	39.0	28.0	39.0
14-15	36.402818509310464	40.0	36.0	41.0	28.0	41.0
16-17	36.37074972093967	40.0	36.0	41.0	28.0	41.0
18-19	36.380684902836265	40.0	36.0	41.0	28.0	41.0
20-21	36.35861313359379	40.0	36.0	41.0	27.5	41.0
22-23	36.29223895174793	40.0	36.0	41.0	27.0	41.0
24-25	36.208225620275	40.0	36.0	41.0	27.0	41.0
26-27	36.073089388096804	40.0	36.0	41.0	27.0	41.0
28-29	35.870273098584406	40.0	35.0	41.0	27.0	41.0
30-31	35.81849979704703	40.0	35.0	41.0	27.0	41.0
32-33	35.6841056116495	40.0	35.0	41.0	27.0	41.0
34-35	35.55723368765538	40.0	35.0	41.0	26.0	41.0
36-37	35.41453872596276	39.0	35.0	41.0	25.5	41.0
38-39	35.23097950175047	39.0	35.0	41.0	25.0	41.0
40-41	35.01670746866914	39.0	35.0	41.0	24.0	41.0
42-43	34.836087891318684	39.0	34.5	41.0	24.0	41.0
44-45	34.59896874524329	39.0	34.0	41.0	23.0	41.0
46-47	34.317456809072	39.0	34.0	41.0	21.5	41.0
48-49	34.04418064082399	38.0	33.0	41.0	19.5	41.0
50-51	33.81765437109949	38.0	33.0	40.0	18.0	41.0
52-53	33.602379940636254	38.0	33.0	40.0	16.0	41.0
54-55	33.36744412451165	38.0	33.0	40.0	14.5	41.0
56-57	33.09228588462124	37.0	33.0	40.0	13.0	41.0
58-59	32.93900438885788	37.0	32.5	40.0	13.0	41.0
60-61	32.812196839007555	36.5	33.0	40.0	12.0	41.0
62-63	32.54617401948349	36.0	32.0	39.5	9.0	41.0
64-65	32.24420696128672	35.5	32.0	39.0	8.0	41.0
66-67	31.941621213658735	35.0	32.0	39.0	7.0	41.0
68-69	31.625377048556498	35.0	32.0	38.0	7.0	40.0
70-71	31.325751560200924	35.0	31.0	37.0	3.5	40.0
72-73	31.01997881678421	35.0	31.0	37.0	2.0	39.0
74-75	30.74070665685727	35.0	31.0	36.0	2.0	39.0
76-77	30.460290983814502	35.0	31.0	36.0	2.0	38.0
78-79	30.19439120452585	35.0	31.0	35.0	2.0	37.0
80-81	29.98162863412654	35.0	31.0	35.0	2.0	37.0
82-83	29.809894908417476	35.0	30.5	35.0	2.0	36.0
84-85	29.629832817494545	35.0	30.0	35.0	2.0	36.0
86-87	29.49776688314983	35.0	30.0	35.0	2.0	36.0
88-89	29.40332684306662	34.5	30.0	35.0	2.0	35.0
90-91	29.310211705312295	34.0	30.0	35.0	2.0	35.0
92-93	29.37184122735806	35.0	30.0	35.0	2.0	35.0
94-95	29.34736414835862	35.0	30.0	35.0	2.0	35.0
96-97	29.32973387792379	35.0	30.0	35.0	2.0	35.0
98-99	29.28997095235679	35.0	30.0	35.0	2.0	35.0
100-101	29.232674793241664	35.0	30.0	35.0	2.0	35.0
102-103	28.3447952077731	33.5	28.5	35.0	2.0	35.0
104-105	29.066990017251	34.0	30.0	35.0	2.0	35.0
106-107	29.174919770155768	35.0	30.0	35.0	2.0	35.0
108-109	29.176993378659496	35.0	30.5	35.0	2.0	35.0
110-111	29.142299837637626	35.0	30.5	35.0	2.0	35.0
112-113	29.099040413009284	35.0	30.5	35.0	2.0	35.0
114-115	29.03089039271399	35.0	30.0	35.0	2.0	35.0
116-117	28.950022197980616	35.0	30.0	35.0	2.0	35.0
118-119	28.84811570855954	35.0	30.0	35.0	2.0	35.0
120-121	28.71349890912781	35.0	30.0	35.0	2.0	35.0
122-123	28.590252866710642	35.0	30.0	35.0	2.0	35.0
124-125	27.647849015678116	34.0	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	33620.0
3	12066.0
4	6717.0
5	5607.0
6	6019.0
7	6966.0
8	7885.0
9	8528.0
10	9530.0
11	10241.0
12	10415.0
13	11953.0
14	14756.0
15	12125.0
16	7148.0
17	6056.0
18	5350.0
19	4895.0
20	4750.0
21	5095.0
22	5965.0
23	8068.0
24	11569.0
25	15874.0
26	19047.0
27	21272.0
28	24842.0
29	29496.0
30	34181.0
31	41563.0
32	51641.0
33	66527.0
34	92670.0
35	146062.0
36	287394.0
37	418676.0
38	111984.0
39	167.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.7160155627396	17.54529342698409	12.780204560997774	24.95848644927854
2	17.250892845250938	19.638536412420617	37.010377998111245	26.1001927442172
3	19.846555426222594	23.734050549806053	28.728262283019284	27.69113174095207
4	14.07720136332513	15.471176562788536	34.31449507028891	36.13712700359742
5	14.64790287045736	36.85755048468982	32.94412666069937	15.550419984153457
6	34.286944915974594	34.28485157584108	16.291387871821613	15.136815636362714
7	29.96936270623085	31.272748032045467	19.84491059365307	18.91297866807061
8	28.227259119439413	33.29553123366772	18.761829012730935	19.715380634161935
9	27.871150324832445	14.400704274037695	18.310744428566988	39.41740097256287
10-11	26.876453268959256	24.576815690294033	26.124590737895925	22.422140302850785
12-13	27.174106078211498	22.14841232792608	26.70155291479138	23.975928679071043
14-15	24.622450099227553	23.062081755385847	24.292103846122135	28.02336429926447
16-17	24.43061969394768	26.16512338092956	24.768570568652457	24.635686356470305
18-19	24.523497075748917	24.980100124124487	26.866227801728105	23.630174998398495
20-21	25.543650107751613	23.879977862950096	26.805081372687383	23.77129065661091
22-23	25.38547776599068	23.574947711267885	26.686424592433315	24.353149930308117
24-25	24.79724406701824	24.039850912702992	26.670667023569543	24.49223799670922
26-27	24.430393034593934	24.14204947825737	26.820422411258722	24.607135075889982
28-29	24.6138573981659	24.196261074612003	26.763969432722973	24.42591209449913
30-31	24.80262164634417	24.103820443106947	26.6393219040176	24.45423600653128
32-33	24.522775688636848	24.2324561055756	26.540170125880213	24.704598079907342
34-35	24.74320811838197	24.199667284627058	26.67311586836651	24.384008728624465
36-37	24.88942368378821	24.09060375651834	26.51827165396036	24.501700905733095
38-39	24.917051002571966	24.258502093825854	26.348680444082017	24.47576645952016
40-41	24.87718614995944	24.216417508101433	26.335380600834853	24.571015741104283
42-43	24.89336971982297	24.225219896707614	26.226085687963124	24.65532469550629
44-45	24.886621656809726	24.341955236051188	26.015822063160755	24.75560104397833
46-47	24.92229484570611	24.391965096747676	25.824657843589993	24.861082213956227
48-49	25.10186908803259	24.70555734055311	25.28364280750477	24.908930763909527
50-51	25.07308130840677	24.940223501781638	25.182247355292347	24.80444783451924
52-53	25.2126438440412	24.763869148983748	25.25080031857326	24.772686688401794
54-55	24.939603931191776	24.782131707674463	25.37951123032271	24.89875313081105
56-57	25.011243880886546	24.773710950112694	25.246746886761272	24.96829828223949
58-59	25.05971393765012	24.6282765753585	25.327082677201567	24.98492680978982
60-61	25.016009309663346	24.721545850694593	25.568588196151232	24.693856643490832
62-63	24.91709768469811	24.787720910287245	25.434953677786176	24.86022772722847
64-65	24.977798330180693	24.53820526775848	25.521612374068958	24.962384027991867
66-67	24.978227596307605	24.67855178787431	25.524139300251118	24.81908131556697
68-69	24.955506998191733	24.77537813897295	25.339648680529937	24.929466182305376
70-71	24.81706241825215	24.587470678607087	25.528482823277475	25.066984079863293
72-73	25.065923142844998	24.833186062163445	25.20341499958451	24.897475795407043
74-75	24.878381730078168	24.97603791813119	25.139062538654915	25.006517813135726
76-77	24.97944045693089	24.697965802494547	25.119654479157717	25.20293926141685
78-79	24.89346759873398	24.795896163184104	25.156650368811146	25.153985869270773
80-81	24.904791070041483	24.965245211959008	25.08532883278784	25.04463488521167
82-83	25.016618333954938	24.80866715887381	25.120134063017733	25.05458044415352
84-85	24.85418861634115	24.922122924943054	24.962718590214035	25.26096986850176
86-87	24.767425892325633	24.99060442785961	25.05118009971834	25.190789580096418
88-89	24.735666890803213	24.783528501287726	25.148659621166946	25.33214498674211
90-91	24.720675839352097	24.776906982652076	25.126409069039983	25.37600810895585
92-93	24.80176455943892	24.895669313188687	25.0309923744012	25.271573752971193
94-95	24.709248854818515	24.73810831621851	25.207185561896882	25.34545726706609
96-97	24.66361427474191	24.699734316322772	25.217909451907705	25.418741957027613
98-99	24.684022391155164	24.763016793366376	25.001585596190512	25.55137521928795
100-101	24.59917300591861	24.76894211612296	25.20451262976887	25.427372248189563
102-103	24.58930926070276	24.63843168494672	25.28063840758356	25.49162064676696
104-105	24.501253743233008	24.81041632774215	25.105435255189946	25.5828946738349
106-107	24.576192176528824	24.67002639027803	25.08202169444485	25.671759738748296
108-109	24.520442582992242	24.652925681644096	25.150601597599636	25.676030137764027
110-111	24.59908480026384	24.722157917936453	25.10745510415709	25.571302177642615
112-113	24.527623919534562	24.749274932567538	25.15746926491086	25.565631882987038
114-115	24.339736446370573	24.864541400575003	25.128291645551638	25.667430507502786
116-117	24.307059102984883	24.816860126773165	25.124738717064716	25.75134205317724
118-119	24.326180045173988	24.669171258382907	25.16347186393762	25.84117683250548
120-121	24.175129982256767	24.670513412075863	25.257641561341	25.896715044326367
122-123	24.100991368730693	24.740012266055437	25.18280164570634	25.976194719507532
124-125	24.06190776003844	24.75171714896971	25.105280936831438	26.08109415416041
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	40.0
1	45.0
2	85.0
3	113.5
4	86.5
5	78.0
6	160.0
7	372.0
8	593.5
9	676.5
10	556.5
11	452.5
12	501.5
13	592.0
14	706.5
15	842.0
16	1017.0
17	1178.5
18	1318.5
19	1500.0
20	1692.5
21	1832.0
22	2068.0
23	2410.5
24	2677.0
25	3039.0
26	3631.5
27	4357.0
28	5217.5
29	6328.0
30	7736.0
31	9142.0
32	10664.0
33	12160.5
34	13644.5
35	15605.5
36	18165.0
37	20811.5
38	23183.0
39	26194.0
40	29443.5
41	32400.0
42	35723.0
43	39873.0
44	44244.5
45	47862.0
46	52100.0
47	56814.5
48	61583.0
49	65802.0
50	67174.5
51	68541.5
52	69077.0
53	69468.5
54	71244.0
55	70974.5
56	67414.5
57	61708.5
58	57672.5
59	53718.5
60	47894.0
61	42302.0
62	36294.5
63	28614.5
64	21780.5
65	17069.0
66	13172.0
67	10206.0
68	8091.5
69	6206.5
70	5037.0
71	4113.0
72	3162.5
73	2669.0
74	2109.0
75	1480.0
76	972.0
77	569.5
78	327.5
79	180.5
80	86.5
81	38.5
82	19.5
83	10.0
84	7.0
85	3.5
86	3.0
87	2.5
88	1.0
89	1.0
90	1.0
91	1.5
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12259627581308033
2	0.0012050332335481252
3	0.016172814450251156
4	0.05498756913085393
5	0.02226140341975747
6	0.018519458115581713
7	0.013318788370795066
8	0.005581206555380791
9	0.003868790907707139
10-11	0.006469125780100462
12-13	0.004566441727129738
14-15	0.002600334872393323
16-17	0.010115936881627683
18-19	0.0048835557359581915
20-21	0.011257547313410117
22-23	0.008720635242782486
24-25	0.012304023542544014
26-27	0.008149830026891267
28-29	0.012526003348723933
30-31	0.019407377340301384
32-33	0.011511238520472882
34-35	0.018868283525293014
36-37	0.013128519965497997
38-39	0.018995129128824395
40-41	0.009323151859556547
42-43	0.008086407225125578
44-45	0.015887411842305545
46-47	0.01572885483789132
48-49	0.019629357146481303
50-51	0.014111573392866205
52-53	0.02007331675884114
54-55	0.015950834644071238
56-57	0.019470800142067077
58-59	0.015570297833477091
60-61	0.01861459231823025
62-63	0.02007331675884114
64-65	0.01661677406261099
66-67	0.011606372723121415
68-69	0.022102846415343245
70-71	0.01433355319904612
72-73	0.016680196864376683
74-75	0.01718757927850221
76-77	0.012779694555786696
78-79	0.027842609975138263
80-81	0.020200162362372518
82-83	0.009386574661322238
84-85	0.012494291947841088
86-87	0.011384392916941499
88-89	0.01902684052970724
90-91	0.01214546653812979
92-93	0.007769293216297123
94-95	0.007230199401288752
96-97	0.0020929524582677965
98-99	0.0016489928459079608
100-101	0.010591607894870363
102-103	0.0029808716829874678
104-105	0.0026320462732761685
106-107	1.902684052970724E-4
108-109	0.018551169516464558
110-111	9.513420264853621E-4
112-113	0.00903774925161094
114-115	0.002473489268861941
116-117	0.007896138819828504
118-119	0.009449997463087929
120-121	0.003963925110355675
122-123	6.976508194225989E-4
124-125	0.021722309604749097
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1576720.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.13311935591283
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.58697134820754	47.062258759091705
2	10.514616438073936	12.435241376285383
3	3.654990119576011	6.483929009567111
4	1.8022434957282552	4.26289118965266
5	1.0816332875840418	3.1980175147017764
6	0.7242345913285408	2.5695750318426818
7	0.5137734886726747	2.12667203193095
8	0.348595055453095	1.6490810416791135
9	0.27005151052204984	1.4372089383550433
>10	1.4332320965481922	14.678552769118063
>50	0.05467456611388218	2.175570164529444
>100	0.014445050128337506	1.4140155384603865
>500	2.15580825379966E-4	0.07553170256738541
>1k	3.23371238069949E-4	0.4314549322182547
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2871	0.1820868638692983	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2487	0.15773250799127303	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.34228017656908E-5
2	0.0	0.0	0.0	0.0	6.34228017656908E-5
3	0.0	0.0	0.0	0.0	6.34228017656908E-5
4	0.0	0.0	0.0	0.0	1.268456035313816E-4
5	0.0	0.0	0.0	0.0	1.268456035313816E-4
6	0.0	0.0	0.0	0.0	1.268456035313816E-4
7	0.0	0.0	0.0	0.0	1.268456035313816E-4
8	0.0	0.0	0.0	0.0	1.268456035313816E-4
9	0.0	0.0	0.0	0.0	1.268456035313816E-4
10-11	0.0	0.0	0.0	0.0	1.268456035313816E-4
12-13	0.0	0.0	0.0	0.0	2.536912070627632E-4
14-15	0.0	0.0	0.0	0.0	2.854026079456086E-4
16-17	0.0	0.0	0.0	0.0	3.805368105941448E-4
18-19	0.0	0.0	0.0	0.0	3.805368105941448E-4
20-21	0.0	0.0	0.0	0.0	3.805368105941448E-4
22-23	0.0	0.0	0.0	0.0	4.7567101324268103E-4
24-25	0.0	0.0	0.0	3.17114008828454E-5	5.708052158912172E-4
26-27	0.0	0.0	0.0	1.902684052970724E-4	5.708052158912172E-4
28-29	0.0	0.0	0.0	7.293622203054442E-4	5.708052158912172E-4
30-31	0.0	0.0	0.0	0.002854026079456086	5.708052158912172E-4
32-33	0.0	0.0	0.0	0.008435232634836877	5.708052158912172E-4
34-35	0.0	0.0	0.0	0.01468237860875742	6.34228017656908E-4
36-37	0.0	0.0	0.0	0.025971637323050384	6.34228017656908E-4
38-39	0.0	0.0	0.0	0.04192247196712162	6.659394185397534E-4
40-41	0.0	0.0	0.0	0.06117129230300877	6.976508194225989E-4
42-43	0.0	3.17114008828454E-5	0.0	0.08010299863006748	7.293622203054442E-4
44-45	0.0	6.34228017656908E-5	0.0	0.10169846263128521	7.92785022071135E-4
46-47	0.0	6.34228017656908E-5	0.0	0.12938251560200922	8.244964229539804E-4
48-49	0.0	6.34228017656908E-5	0.0	0.15760566238774165	0.0011416104317824344
50-51	0.0	6.34228017656908E-5	0.0	0.18750951342026487	0.0011733218326652798
52-53	0.0	6.34228017656908E-5	0.0	0.21557410320158304	0.001268456035313816
54-55	0.0	1.268456035313816E-4	0.0	0.25086889238418997	0.0013953016388451975
56-57	0.0	1.268456035313816E-4	0.0	0.286544218377391	0.0015221472423765793
58-59	0.0	1.268456035313816E-4	0.0	0.3294814551727637	0.00158557004414227
60-61	0.0	1.268456035313816E-4	0.0	0.3796171799685423	0.00158557004414227
62-63	0.0	1.268456035313816E-4	0.0	0.4291820995484297	0.0017124156476736516
64-65	0.0	1.268456035313816E-4	0.0	0.48118879699629613	0.0017124156476736516
66-67	0.0	1.268456035313816E-4	0.0	0.543152874321376	0.001744127048556497
68-69	0.0	1.268456035313816E-4	0.0	0.614757217514841	0.0017758384494393424
70-71	0.0	1.268456035313816E-4	0.0	0.6921013242681009	0.0017758384494393424
72-73	0.0	1.268456035313816E-4	0.0	0.7736313359378963	0.0019343954538535695
74-75	0.0	1.268456035313816E-4	0.0	0.8566517834491857	0.001966106854736415
76-77	0.0	1.268456035313816E-4	0.0	0.9456656857273327	0.001966106854736415
78-79	0.0	1.268456035313816E-4	0.0	1.0496790806230658	0.001966106854736415
80-81	0.0	1.268456035313816E-4	0.0	1.1673918007001878	0.0019978182556192603
82-83	0.0	1.268456035313816E-4	0.0	1.2918590491653559	0.0022515094626820234
84-85	0.0	1.268456035313816E-4	0.0	1.4189583439038003	0.002283220863564869
86-87	0.0	1.268456035313816E-4	0.0	1.560137500634228	0.002283220863564869
88-89	0.0	1.268456035313816E-4	0.0	1.7191384646608148	0.002283220863564869
90-91	0.0	1.268456035313816E-4	0.0	1.895327007965904	0.002283220863564869
92-93	0.0	1.268456035313816E-4	0.0	2.0872126947080014	0.002283220863564869
94-95	0.0	1.268456035313816E-4	0.0	2.2844893196001825	0.002378355066213405
96-97	0.0	1.268456035313816E-4	0.0	2.495750672281699	0.002473489268861941
98-99	0.0	1.268456035313816E-4	0.0	2.7245167182505456	0.002473489268861941
100-101	0.0	1.268456035313816E-4	0.0	2.9827109442386726	0.002473489268861941
102-103	0.0	1.268456035313816E-4	0.0	3.2648155664924654	0.0025686234715104773
104-105	0.0	1.268456035313816E-4	0.0	3.561634278755898	0.0026003348723933227
106-107	0.0	1.268456035313816E-4	0.0	3.8702496321477495	0.0026637576741590134
108-109	0.0	1.268456035313816E-4	0.0	4.198018671672839	0.002822314678573241
110-111	0.0	1.268456035313816E-4	0.0	4.553408341366888	0.003012583083870313
112-113	0.0	1.268456035313816E-4	0.0	4.936640621036075	0.0030442944847531585
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2215	0.0	77.52641	1
GTATCAA	4570	0.0	61.451946	1
TCAACGC	6285	0.0	45.002827	4
CAACGCA	6485	0.0	43.676235	5
ATCAACG	6570	0.0	42.96002	3
TATCAAC	6625	0.0	42.693245	2
AACGCAG	6865	0.0	41.16671	6
TATACCG	200	1.8189894E-12	35.702568	5
ACGCAGA	8035	0.0	35.09492	7
GTACATG	8015	0.0	34.14602	1
CGCAGAG	8465	0.0	33.311134	8
TACATGG	8350	0.0	32.447136	2
ACATGGG	8505	0.0	31.435722	3
GCAGAGT	9095	0.0	30.480438	9
CATGGGG	7365	0.0	29.510159	4
GTGGTAT	870	0.0	29.47	1
AGAGTAC	8265	0.0	27.46062	10-11
CAGAGTA	8645	0.0	25.737436	10-11
ATGGGGG	4000	0.0	25.586842	5
GAGTACT	4800	0.0	24.540848	12-13
>>END_MODULE