FastQCFastQC Report
Mon 15 Aug 2016
SRR2921502_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921502_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2036980
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20850.069.354791
GTATCAA41300.055.9059371
TCAACGC52850.043.565854
CAACGCA53700.042.7654655
ATCAACG57250.040.3214763
AACGCAG56550.040.1844146
TATCAAC57950.039.7317542
GTACATG104100.038.3001371
TACATGG107300.036.8170132
ACATGGG111950.034.8626143
ACGCAGA65600.034.5499957
CATGGGG97700.032.7009164
CGCAGAG72800.031.0512498
GCAGAGT78300.028.490269
ATGGGGG55350.026.7646715
AGAGTAC70450.025.3740810-11
GTATATA6850.025.193221
CAGAGTA75100.024.23864610-11
ATGGGGA36300.023.2735125
GTATAGG6250.022.851121
Produced by FastQC (version 0.11.5)