FastQCFastQC Report
Mon 15 Aug 2016
SRR2921502_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921502_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2036980
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25970.1274926607035906No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22660.1112431148072146No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28350.079.5039751
GTATCAA55650.057.1099971
TCAACGC72750.042.5588954
CAACGCA73850.041.9839365
ATCAACG76650.040.705193
AACGCAG77000.040.33286
TATCAAC78950.039.670192
GTACATG103650.034.9771421
ACGCAGA90050.034.3531237
TACATGG107800.033.0303422
CGCAGAG96700.031.989118
ACATGGG112100.031.4446493
CATGGGG95050.029.2539634
GCAGAGT103400.029.1684069
AGAGTAC96150.026.6048310-11
CAGAGTA99450.026.2304710-11
ATGGGGG52650.025.3189435
GAGTACT52050.022.345712-13
AGTACTT55350.022.24952112-13
GTGGTAT11900.022.047321
Produced by FastQC (version 0.11.5)