FastQCFastQC Report
Mon 15 Aug 2016
SRR2921501_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921501_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2046099
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21500.067.820171
GTATCAA40500.057.458321
TCAACGC53200.042.496634
CAACGCA54100.041.679695
AACGCAG57900.038.838636
ATCAACG59600.038.131953
GTACATG103350.038.0646821
TACATGG107800.035.4305232
TATCAAC64700.035.2172782
ACATGGG109250.034.797763
ACGCAGA67700.032.8649947
CATGGGG89050.031.4680274
CGCAGAG72650.030.3778558
GCAGAGT79550.026.7695089
GTATAGG8100.025.716641
ATGGGGG50050.025.5574135
AGAGTAC70950.025.19510510-11
CAGAGTA76550.023.58509410-11
GTATACG1802.1736982E-523.1449781
TATAGGA11450.021.8225292
Produced by FastQC (version 0.11.5)